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21. 发明申请

US20160009759A1 PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES 审中-公开
标题翻译：非离子有机聚合物和电镀表面存在的蛋白质纯化
公开(公告)号：US20160009759A1
公开(公告)日：2016-01-14
申请号：US14769101
申请日：2014-02-26
申请人： Agency for Science, Technology and Research
发明人： Peter Stanley GAGNON
IPC分类号： C07K1/16
CPC分类号： C07K1/165 , B01D15/305 , B01D15/363 , B01D15/3847 , C07K1/30 , C07K1/303 , B01D15/327 , B01D15/34
摘要： A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.
摘要翻译：从制剂中纯化所需蛋白质的方法包括（a）提供具有少于约5％染色质残留在原始生产培养基中的形式的制剂，（b）使制剂与非离子有机聚合物和盐接触，非离子有机聚合物的浓度足以沉淀所需蛋白质或使其在亲水表面上的沉积，或将其保持在沉淀状态或在亲水表面上增加，盐浓度足以产生大于生理电导率，和（ c）任选地在足以产生大于生理电导率的盐浓度的情况下任选地将制剂与至少一个正电性表面接触，所需蛋白质基本上不吸附至至少一个正电面，同时不阻止酸性污染物的吸附所述至少一个正电面。

22. 发明申请

US20160002290A1 MATERIALS AND METHODS FOR REMOVING ENDOTOXINS FROM PROTEIN PREPARATIONS 有权
标题翻译：从蛋白质制备中去除内毒素的材料和方法
公开(公告)号：US20160002290A1
公开(公告)日：2016-01-07
申请号：US14769245
申请日：2014-02-20
申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
发明人： Peter Stanley GAGNON , Vincent VAGENENDE
IPC分类号： C07K1/36 , C07K1/34 , C07K1/30 , C07K1/18
CPC分类号： C07K1/36 , B01D15/34 , B01D15/363 , B01D15/3847 , C07K1/18 , C07K1/30 , C07K1/34
摘要： A method includes (i) adding allantoin in a supersaturating amount to a protein preparation including a desired protein and at least one endotoxin as a contaminant, (ii) removing solids after the adding step to provide a sample for further purification by void exclusion chromatography on a packed particle bed of electropositive particles in a column, the packed particle bed having an interparticle volume, (iii) applying a sample volume to the packed particle bed, wherein the electropositive particles support void exclusion chromatography, and wherein the sample volume is not greater than the interparticle volume, and (iv) eluting a purified sample including the desired protein and a reduced amount of the endotoxin. The method is optionally carried out with only the allantoin treatment or only the void exclusion chromatography.
摘要翻译：一种方法包括（i）将超滤量的尿囊素加入到包含所需蛋白质和至少一种作为污染物的内毒素的蛋白质制剂中，（ii）在加入步骤后除去固体，以提供样品进一步通过空隙排阻色谱法进行纯化在柱中的正电性颗粒的填充颗粒床，所述填充颗粒床具有颗粒间体积，（iii）将样品体积施加到所述填充颗粒床，其中所述正电荷颗粒支持空隙排阻层析，并且其中所述样品体积不大（iv）洗脱含有所需蛋白质和减少量内毒素的纯化样品。该方法任选仅通过尿囊素处理或仅进行空白排阻色谱进行。

23. 发明申请

US20150376231A1 PROTEIN PURIFICATION METHODS 有权
标题翻译：蛋白纯化方法
公开(公告)号：US20150376231A1
公开(公告)日：2015-12-31
申请号：US14766131
申请日：2014-02-05
申请人： AGENCY FOR SCIENCE TECHNOLOGY AND RESEARCH
发明人： Peter Stanley GAGNON
IPC分类号： C07K1/30 , C07K16/00 , B01D15/36 , C07K16/28
CPC分类号： C07K1/303 , B01D15/36 , C07K1/30 , C07K16/00 , C07K16/2863 , C07K16/32
摘要： A method for the purification of a desired protein from a protein preparation includes conditioning the protein preparation by treatment with soluble organic multivalent ions, immobilized organic multivalent ions, or both, optionally in the presence of supersaturated allantoin, thereby removing at least 90% of chromatin, then (1) precipitating the desired protein with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration to provide a precipitate of the desired protein; or (2) precipitating the desired protein with a nonionic organic polymer in the absence of non-precipitating salts at greater than physiological concentration to provide a precipitate and subsequently washing the precipitate with a nonionic organic polymer in the presence of non-protein-precipitating salts at greater than physiological concentration.
摘要翻译：用于从蛋白质制剂中纯化所需蛋白质的方法包括通过用可溶性有机多价离子，固定的有机多价离子或两者处理，任选地在过饱和尿囊素存在下调节蛋白质制剂，由此除去至少90％的染色质，然后（1）在非蛋白质沉淀盐存在下，用非离子有机聚合物在大于生理浓度下沉淀所需蛋白质以提供所需蛋白质的沉淀物; 或（2）在不存在非沉淀盐的情况下，用非离子有机聚合物在大于生理浓度下沉淀所需蛋白质以提供沉淀物，随后在非蛋白质沉淀盐存在下用非离子有机聚合物洗涤沉淀物大于生理浓度。

24. 发明申请

US20140343255A1 TWO-STAGE ULTRAFILTRATION/DIAFILTRATION 有权
标题翻译：两级超声波/双相
公开(公告)号：US20140343255A1
公开(公告)日：2014-11-20
申请号：US14294460
申请日：2014-06-03
申请人： GRIFOLS THERAPEUTICS INC.
发明人： Martin Gonzalez , Woody D. Wood , Fred H. Earp
IPC分类号： C07K16/06
CPC分类号： C07K16/065 , C07K1/30 , C07K1/34 , C07K1/36
摘要： The present invention provides a method for concentrating a protein, in particular a method for concentrating a plasma product, in particular IgG, using glycine in a two-stage ultrafiltration/diafiltration approach.
摘要翻译：本发明提供一种浓缩蛋白质的方法，特别是使用甘氨酸在两级超滤/渗滤方法中浓缩血浆产物，特别是IgG的方法。

25. 发明申请

US20140342466A1 Nanoporous substrates for analytical methods 审中-公开
标题翻译：用于分析方法的纳米多孔基材
公开(公告)号：US20140342466A1
公开(公告)日：2014-11-20
申请号：US14288532
申请日：2014-05-28
申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION , THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
发明人： Mauro FERRARI , Mark Ming-Cheng CHENG
IPC分类号： G01N1/40 , B01L3/00
CPC分类号： G01N1/405 , B01D15/00 , B01D15/08 , B01J20/103 , B01J20/2808 , B01J2220/54 , B01L3/5023 , B82Y5/00 , C07K1/30 , C07K1/34 , G01N33/6803 , Y10T436/2525 , Y10T436/255
摘要： Nanoporous materials can be used to enrich samples for subsequent analysis of substances contained in the sample. The method is shown to enrich the yield of species in the low molecular weight proteome, allowing detection of small peptides in the low nanomolar range.
摘要翻译：可以使用纳米多孔材料来富集样品，以便随后分析样品中所含的物质。该方法显示为丰富低分子量蛋白质组中物种的产量，允许检测低纳摩尔范围内的小肽。

26. 发明申请

US20140141021A1 METHOD FOR PREPARING A CONCENTRATE OF POLYVALENT IMMUNOGLOBULIN 有权
标题翻译：制备多价免疫球蛋白浓度的方法
公开(公告)号：US20140141021A1
公开(公告)日：2014-05-22
申请号：US14131944
申请日：2012-07-11
申请人： Abdessatar Chtourou , Damien Bataille , Georges Michaux
发明人： Abdessatar Chtourou , Damien Bataille , Georges Michaux
IPC分类号： C07K1/36 , C07K16/06
CPC分类号： C07K1/36 , C07K1/165 , C07K1/30 , C07K16/06 , C07K16/065
摘要： The present invention relates to a method for preparing a concentrate of polyvalent immunoglobulins with view to therapeutic use, from an initial solution of blood plasma or a plasma fraction enriched with immunoglobulins, comprising the steps for removing the protein contaminants by precipitation with caprylic acid in order to obtain a solution free of proteases, and for separating by chromatography on a fluidized bed the solution free of proteases, said method allowing a concentrate of human polyvalent immunoglobulins with a yield of more than 4.5 g of immunoglobulins per liter of blood plasma applied to be obtained.
摘要翻译：本发明涉及从血浆的初始溶液或富含免疫球蛋白的血浆级分获得治疗用途的多价免疫球蛋白浓缩物的制备方法，包括以下步骤：用辛酸沉淀除去蛋白质污染物以获得不含蛋白酶的溶液，并且在流化床上通过色谱分离不含蛋白酶的溶液，所述方法允许人多价免疫球蛋白的浓缩物，每升血浆中产生超过4.5g的免疫球蛋白，其产生量为获得。

27. 发明授权

US08691918B2 Stimulus responsive polymers for the purification of biomolecules 有权
标题翻译：用于生物分子纯化的刺激响应聚合物
公开(公告)号：US08691918B2
公开(公告)日：2014-04-08
申请号：US13108576
申请日：2011-05-16
申请人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
发明人： Jad Jaber , Wilson Moya , James Hamzik , Arezki Boudif , Yu Zhang , Neil Soice , John Charkoudian , Nripen Singh
IPC分类号： C08F16/00
CPC分类号： B01J41/14 , B01J41/13 , C07K1/30 , C07K1/32 , C07K16/00 , C07K2317/10 , C08F8/00 , C08F8/02 , C08F126/02 , C08F26/02
摘要： The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.
摘要翻译：本发明提供了新的和改进的刺激响应聚合物以及使用其进行生物分子纯化的方法。

28. 发明申请

US20130224183A1 FRACTION I-IV-1 PRECIPITATION OF IMMUNOGLOBINS FROM PLASMA 有权
标题翻译：从等离子体中分离I-IV-1免疫球蛋白
公开(公告)号：US20130224183A1
公开(公告)日：2013-08-29
申请号：US13776448
申请日：2013-02-25
申请人： Baxter International Inc. , Baxter Healthcare S.A.
发明人： Leopold Bruckschwaiger , Thomas Gundinger , Julia Nuernberger , Wolfgang Teschner , Hans-Peter Schwarz
IPC分类号： A61K39/395
CPC分类号： A61K39/395 , A61K35/16 , A61K39/39591 , C07K1/145 , C07K1/30 , C07K16/065
摘要： Among other aspects, the present invention provides methods for the manufacture of blood protein compositions from pooled plasma. In one embodiment, the invention provides an alcohol fractionation scheme that allows for significant increases in the yield of blood proteins purified from the starting plasma sample. In a specific embodiment, a method for fractionating pooled plasma is provided, the method comprising an initial low pH, high alcohol precipitation step. The present invention also provides pharmaceutical compositions of therapeutic blood proteins.
摘要翻译：在其它方面中，本发明提供了从合并血浆制造血液蛋白质组合物的方法。在一个实施方案中，本发明提供了允许从起始血浆样品纯化的血液蛋白的产量显着提高的醇分馏方案。在一个具体实施方案中，提供了用于分级汇集的等离子体的方法，该方法包括初始低pH，高醇沉淀步骤。本发明还提供治疗性血液蛋白质的药物组合物。

29. 发明申请

US20130195897A1 REMOVAL OF SERINE PROTEASES BY TREATMENT WITH FINELY DIVIDED SILICON DIOXIDE 有权
标题翻译：用精细二氧化硅二氧化硅处理去除丝氨酸蛋白
公开(公告)号：US20130195897A1
公开(公告)日：2013-08-01
申请号：US13830815
申请日：2013-03-14
申请人： Baxter International Inc. , Baxter Healthcare S.A.
发明人： Wolfgang Teschner , Hans-Peter Schwarz , Ruth Madlener , Sonja Svatos , Azra Pljevljakovic , Alfred Weber
IPC分类号： A61K39/395
CPC分类号： C07K16/065 , A61K9/0019 , A61K9/08 , A61K35/16 , A61K38/1709 , A61K39/39525 , A61K47/183 , B01D15/12 , B01D15/362 , B01D15/363 , B01D15/424 , C07K1/30 , C07K1/36
摘要： The present invention provides novel methods for reducing the serine protease and/or serine protease zymogen content of a plasma-derived protein composition. Also provided are methods for manufacturing plasma-derived protein compositions having reduced serine protease and\or serine protease zymogen content. Among yet other aspects, the present invention provides aqueous and lyophilized compositions of plasma-derived proteins having reduced serine protease and/or serine protease zymogen content. Yet other aspects include methods for treating, managing, and/or preventing a disease comprising the administration of a plasma-derived protein composition having a reduced serine protease or serine protease zymogen content.
摘要翻译：本发明提供了用于降低血浆来源的蛋白质组合物的丝氨酸蛋白酶和/或丝氨酸蛋白酶酶原含量的新方法。还提供了制备具有降低的丝氨酸蛋白酶和/或丝氨酸蛋白酶酶原含量的血浆衍生蛋白质组合物的方法。在其它方面中，本发明提供具有降低的丝氨酸蛋白酶和/或丝氨酸蛋白酶酶原含量的血浆衍生蛋白质的水性和冻干组合物。还有其它方面包括用于治疗，控制和/或预防疾病的方法，所述疾病包括施用具有降低的丝氨酸蛋白酶或丝氨酸蛋白酶酶原含量的血浆衍生的蛋白质组合物。

30. 发明授权

US08362217B2 Purification of proteins 有权
标题翻译：蛋白质的纯化
公开(公告)号：US08362217B2
公开(公告)日：2013-01-29
申请号：US12004319
申请日：2007-12-20
申请人： Wilson Moya , Jad Jaber
发明人： Wilson Moya , Jad Jaber
IPC分类号： A61K39/395 , C07K1/32
CPC分类号： C07K1/32 , C07K1/30
摘要： The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
摘要翻译：本发明涉及能够在含有各种生物材料的混合物中结合所需生物分子的选择性可溶性聚合物以及使用这种聚合物从这种混合物中纯化生物分子的方法。聚合物在一定的工艺条件例如pH或温度和/或盐浓度下可溶于混合物中，并且在工艺条件改变时变得不溶解并沉淀出溶液。聚合物能够结合所需的生物分子（蛋白质，多肽等），并且即使在聚合物从溶液中沉淀出来之后仍然能够结合该生物分子。然后可以从流的其余部分过滤出沉淀物，并回收所需的生物分子，例如通过洗脱和进一步处理。

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