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21. 发明授权

US5681717A DNA encoding novel cell surface protein 失效
标题翻译：编码新型细胞表面蛋白的DNA
公开(公告)号：US5681717A
公开(公告)日：1997-10-28
申请号：US633485
申请日：1996-04-17
申请人： Hisashi Kawasaki , Makoto Tsuchiya , Kiyoshi Miwa , Yoshio Kawahara
发明人： Hisashi Kawasaki , Makoto Tsuchiya , Kiyoshi Miwa , Yoshio Kawahara
IPC分类号： C07K14/345 , C12N15/31 , C12N15/77 , C12P13/04 , C12P21/06 , C07H21/04 , C12P1/04
CPC分类号： C12P13/04 , C07K14/345 , C12N15/77
摘要： A DNA fragment containing a gene coding for a novel cell surface layer protein derived from Brevibacterium lactofermentum and having two sequences of: ##STR1## in the molecule, and having a molecular weight of about 63,000 dalton is introduced into Coryneform bacteria to obtain a transformant, or originate a mutant strain being sufficient in the novel cell surface layer protein, which is used for producing a useful substance such as an L-amino acid by an L-amino acid fermentation method.
摘要翻译：一种含有编码来自乳发酵短杆菌的新型细胞表面蛋白质的基因的DNA片段，具有以下两个序列：（1）Thr-Leu-Arg-Gln-His-Tyr-Ser-Ser-Leu-Ile-Pro-Asn Leu-Phe-Ile-Ala-Ala-Val-Gly-Asn-Ile-Asn-Glu-Leu-Asn-Ala-Asp-Gln-Ala-Ala-Arg-Glu-Leu-Phe-Leu- -Trp-Asp-Thr（SEQ ID NO：1）和：（2）Asn-Lys-Thr-Asp-Phe-Ala-Glu-Ile-Glu-Leu-Tyr-Asp-Val-Leu-Tyr-Thr- 分子中的Asp-Ala-Asp-Ile-Ser-Gly-Asp-Ala-Pro-Leu-Leu-Ala-Pro-Ala-Tyr-Lys（SEQ ID NO：2），分子量约为63,000 导入棒状细菌以获得转化体，或产生新的细胞表面层蛋白中足够的突变菌株，其通过L-氨基酸发酵法用于生产有用物质如L-氨基酸。

22. 发明授权

US5179010A Fermentation process for producing L-lysine 失效
标题翻译：发酵L-赖氨酸的发酵工艺
公开(公告)号：US5179010A
公开(公告)日：1993-01-12
申请号：US469687
申请日：1990-01-26
申请人： Yasuhiko Yoshihara , Yoshio Kawahara , Shigeho Ikeda
发明人： Yasuhiko Yoshihara , Yoshio Kawahara , Shigeho Ikeda
IPC分类号： C12N9/02 , C12P13/08
CPC分类号： C12N9/0089 , C12P13/08 , Y10S435/84 , Y10S435/843
摘要： A fermentative process for producing L-lysine is disclosed. The process is based on growing in a culture medium a mutant strain of the genus Brevibacterium or Corynebacterium (1) capable of producing L-lysine, and (2) having an intensified superoxide dismutase activity.
摘要翻译：公开了生产L-赖氨酸的发酵方法。该方法基于在培养基中生长能够产生L-赖氨酸的短杆菌属或棒状杆菌属（1）的突变菌株，和（2）具有增强的超氧化物歧化酶活性。

23. 发明授权

US07227565B2 Electronic apparatus equipped with image capturing device and microphone 失效
标题翻译：配有摄像装置和麦克风的电子设备
公开(公告)号：US07227565B2
公开(公告)日：2007-06-05
申请号：US10790192
申请日：2004-03-02
申请人： Yoshio Kawahara
发明人： Yoshio Kawahara
IPC分类号： H04N7/14
CPC分类号： H04N7/142 , H04M1/03 , H04M2250/52 , H04N7/147 , H04N2007/145
摘要： In a mobile telephone including a camera 2a and a microphone 4 loaded in front, and a camera 2b on the back, the sensitivity of the microphone 4 is set at a normal level when the camera 2a is selected, and the sensitivity of the microphone 4 is enhanced when the camera 2b is selected so that the conventional problems can be solved. To attain this, an electronic apparatus such as a mobile telephone, a PHS, a PDA, etc. capable of capturing a moving picture and a still image is loaded with a camera and a microphone capable of easily and clearly collecting voice from all directions when a user who is taking a picture captures an image in front of the user, the user himself or herself, or a target in a different direction.
摘要翻译：在包括摄像机2a和装在前面的麦克风4的移动电话和背面的相机2b之间，当选择相机2a时，麦克风4的灵敏度被设置在正常水平，并且灵敏度当选择相机2b时，麦克风4被增强，从而可以解决传统的问题。为了实现这一点，能够捕获运动图像和静止图像的诸如移动电话，PHS，PDA等的电子设备被加载有能够容易且清楚地从各个方向收集语音的相机和麦克风正在拍摄照片的用户在用户面前，用户自身或不同方向上的目标捕获图像。

24. 发明授权

US5164307A Process for producing L-amino acids by fermentation 失效
标题翻译：通过发酵生产L-氨基酸的方法
公开(公告)号：US5164307A
公开(公告)日：1992-11-17
申请号：US675961
申请日：1991-03-27
申请人： Yasuhiko Yoshihara , Yoshio Kawahara , Yasutsugu Yamada , Sigeho Ikeda
发明人： Yasuhiko Yoshihara , Yoshio Kawahara , Yasutsugu Yamada , Sigeho Ikeda
IPC分类号： C12P19/32
CPC分类号： C12P19/32
摘要： A process is provided for producing an L-amino acid, which entails culturing bacteria producing the L-amino acid in a medium containing cane molasses, sucrose or glucose as a main carbon source and containing at least one substance selected from the group consisting of N-methylglycine, N,N-dimethylglycine, N,N,N-trimethylglycine and (2-hydroxyethyl)trimethyl ammonium in an amount effective to enhance the yield of the L-amino acid; and harvesting the L-amino acid, and wherein the L-amino acid is selected from the group consisting of L-glutamic acid, L-lysine, L-glutamine, L-arginine, L-isoleucine, L-valine, L-threonine, L-histidine, L-phenylalanine, L-tryptophan, L-serine, L-ornithine, L-citrulline, L-tyrosine and L-leucine.
摘要翻译：提供了一种生产L-氨基酸的方法，其需要在含有甘蔗糖浆，蔗糖或葡萄糖作为主要碳源的培养基中培养产生L-氨基酸的细菌并含有至少一种选自N - 甲基甘氨酸，N，N-二甲基甘氨酸，N，N，N-三甲基甘氨酸和（2-羟乙基）三甲基铵，其量有效提高L-氨基酸的产率; 收集L-氨基酸，其中L-氨基酸选自L-谷氨酸，L-赖氨酸，L-谷氨酰胺，L-精氨酸，L-异亮氨酸，L-缬氨酸，L-苏氨酸，L-组氨酸，L-苯丙氨酸，L-色氨酸，L-丝氨酸，L-鸟氨酸，L-瓜氨酸，L-酪氨酸和L-亮氨酸。

25. 发明授权

US5164306A Process for producing 5'-inosinic acid by fermentation 失效
标题翻译：通过发酵生产5'-肌苷酸的方法
公开(公告)号：US5164306A
公开(公告)日：1992-11-17
申请号：US472415
申请日：1990-01-31
申请人： Yasuhiko Yoshihara , Yoshio Kawahara , Yasutsugu Yamada , Sigeho Ikeda
发明人： Yasuhiko Yoshihara , Yoshio Kawahara , Yasutsugu Yamada , Sigeho Ikeda
IPC分类号： C12P19/32
CPC分类号： C12P19/32
摘要： A process for producing 5'-inosinic acid by culturing 5'-inosinic acid-producing bacteria in a medium containing inosine, and cane molasses, sucrose or glucose as the main carbon source and containing at least one of L-methylglycine, N,N-dimethylglycine, N,N,N-trimethylglycine and (2-hydroxyethyl)trimethylammonium in an amount effective to enhance the yield of 5'-inosinic acid, and harvesting the 5'-inosinic acid produced.
摘要翻译：一种通过在含有肌苷的培养基中培养5'-肌苷酸产生细菌，以蔗糖，蔗糖或葡萄糖为主要碳源并含有L-甲基甘氨酸，N，N中的至少一种来生产5'-肌苷酸的方法 - 二甲基甘氨酸，N，N，N-三甲基甘氨酸和（2-羟乙基）三甲基铵，其量可以有效提高5'-肌苷酸的产率，并收获所产生的5'-肌苷酸。

26. 发明授权

US4386462A Assembly apparatus having operating units and a power unit interconnected by bowden cables 失效
标题翻译：具有操作单元的组装装置和通过弓形电缆互连的动力单元
公开(公告)号：US4386462A
公开(公告)日：1983-06-07
申请号：US276082
申请日：1981-06-22
申请人： Noriaki Nakanishi , Tadayoshi Nishizono , Masaaki Nakanishi , Toshiaki Nakanishi , Yoshio Kawahara
发明人： Noriaki Nakanishi , Tadayoshi Nishizono , Masaaki Nakanishi , Toshiaki Nakanishi , Yoshio Kawahara
IPC分类号： B23P19/04 , H05K13/04 , B23P19/00
CPC分类号： H05K13/04 , B23P19/04 , Y10T29/53404
摘要： An apparatus for an assembly line for assembling articles comprising a moving table, an operating unit disposed near the table having a member movable in two directions, at least one Bowden cable having a wire therein, one end of the wire being connected to the member, and a pulling mechanism to which the other end of the wire is connected. The return of the wire can be accomplished by a return spring on the member or by a second cable wire pulled by the pulling mechanism. Multiple units can be powered and controlled around the same table by one pulling mechanism.
摘要翻译：一种用于组装物品的装配线的装置，包括移动台，设置在桌子附近的操作单元，其具有可在两个方向上移动的构件，至少一根具有导线的鲍登缆线，所述线的一端连接到构件，以及连接另一端的牵引机构。线的返回可以通过构件上的复位弹簧或由拉动机构拉动的第二电缆线来实现。多个单元可以通过一个拉动机构在同一张桌子上供电和控制。

27. 发明授权

US5547864A Coryneform bacteria deficient in a cell surface protein 失效
标题翻译：棒状细菌缺乏细胞表面蛋白质
公开(公告)号：US5547864A
公开(公告)日：1996-08-20
申请号：US295670
申请日：1994-09-08
申请人： Hisashi Kawasaki , Makoto Tsuchiya , Kiyoshi Miwa , Yoshio Kawahara
发明人： Hisashi Kawasaki , Makoto Tsuchiya , Kiyoshi Miwa , Yoshio Kawahara
IPC分类号： C07K14/345 , C12N15/31 , C12N15/77 , C12P13/04 , C12P1/04 , C07K14/34 , C12N1/20
CPC分类号： C12P13/04 , C07K14/345 , C12N15/77
摘要： A DNA fragment containing a gene coding for a novel cell surface layer protein derived from Brevibacterium lactofermentum and having two sequences of:(1)Thr-Leu-Arg-Gln-His-Tyr-Ser-Ser-Leu-Ile-Pro-Asn-Leu-Phe-Ile-Ala-Ala-Val-Gly-Asn-Ile-Asn-Glu-Leu-Asn-Asn-Ala-Asp-Gln-Ala-Ala-Arg-Glu-Leu-Phe-Leu-Asp-Trp-Asp-Thr (SEQ ID NO: 1) and:(2)Asn-Lys-Thr-Asp-Phe-Ala-Glu-Ile-Glu-Leu-Tyr-Asp-Val-Leu-Tyr-Thr-Asp-Ala-Asp-Ile-Ser-Gly-Asp-Ala-Pro-Leu-Leu-Ala-Pro-Ala-Tyr-Lys (SEQ ID NO: 2)in the molecule, and having a molecular weight of about 63,000 dalton is introduced into Coryneform bacteria to obtain a transformant, or originate a mutant strain being sufficient in the novel cell surface layer protein, which is used for producing a useful substance such as an L-amino acid by an L-amino acid fermentation method.
摘要翻译： PCT No.PCT / JP94 / 00039 Sec。 371日期1994年9月8日 102（e）1994年9月8日PCT PCT 1994年1月13日PCT公布。公开号WO94 / 15963 日期1994年7月21日包含编码来自乳发酵短杆菌的新型细胞表面蛋白质的基因的DNA片段，具有以下两个序列：（1）Thr-Leu-Arg-Gln-His-Tyr-Ser-Ser-Leu- Ile-Pro-Asn-Leu-Phe-Ile-Ala-Ala-Val-Gly-Asn-Ile-Asn-Glu-Leu-Asn-Asn-Ala-Asp-Gln-Ala-Ala-Arg-Glu-Leu- Phe-Leu-Asp-Trp-Asp-Thr（SEQ ID NO：1）和（2）Asn-Lys-Thr-Asp-Phe-Ala-Glu-Ile-Glu-Leu-Tyr-Asp-Val-Leu -Tyr-Thr-Asp-Ala-Asp-Ala-Ser-Gly-Asp-Ala-Pro-Leu-Leu-Ala-Pro-Ala-Tyr-Lys（SEQ ID NO：2）将约63,000道尔顿的分子量引入棒状细菌以获得转化体，或产生在新型细胞表面层蛋白中足够的突变菌株，其用于通过L-乳酸生产有用物质如L-氨基酸，氨基酸发酵法。

28. 发明授权

US5492818A Method of producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US5492818A
公开(公告)日：1996-02-20
申请号：US354014
申请日：1994-12-05
申请人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
发明人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
IPC分类号： C12P13/14 , C12R1/13 , C12R1/15 , C12P13/18
CPC分类号： C12P13/14 , Y10S435/843
摘要： The present invention provides a method of producing L-glutamic acid by fermentation, comprising the steps ofculturing a mutant of an L-glutamic acid-producing microorganism of the genus Brevibacterium or Corynebacterium which has lower .alpha.-ketoglutaric acid dehydrogenase activity compared with the wild strains from which said mutant is derived, in a liquid nutrient culture medium containing biotin at a concentration of 10 to 1000 .mu.g/l without adding a biotin activity-suppressing substance thereto;producing and accumulating L-glutamic acid in the culture solution; andrecovering L-glutamic acid from said culture solution.According to the method of the present invention, it is possible to industrially produce L-glutamic acid by fermentation in a more economical and efficient manner.
摘要翻译：本发明提供通过发酵生产L-谷氨酸的方法，包括以下步骤：与野生型相比，培养具有较低α-酮戊二酸脱氢酶活性的短杆菌属或棒状杆菌属的L-谷氨酸生产微生物的突变体在不含生物素活性抑制物质的情况下，在含有浓度为10〜1000μg/ l的生物素的液体营养培养基中，所述突变体得自其中的菌株; 在培养液中产生和积累L-谷氨酸; 从所述培养液中回收L-谷氨酸。根据本发明的方法，可以以更经济和有效的方式通过发酵工业生产L-谷氨酸。

29. 发明授权

US5362636A Process for producing L-lysine by fermentation with a bacteria having selenalysine resistance 失效
标题翻译：通过用具有硒酸赖氨酸抗性的细菌发酵生产L-赖氨酸的方法
公开(公告)号：US5362636A
公开(公告)日：1994-11-08
申请号：US793950
申请日：1991-10-22
申请人： Manabu Yokomori , Takeshi Niwa , Kazuhiko Totsuka , Yoshio Kawahara , Shigeru Nakamori , Nobuyoshi Esaki , Kenji Soda
发明人： Manabu Yokomori , Takeshi Niwa , Kazuhiko Totsuka , Yoshio Kawahara , Shigeru Nakamori , Nobuyoshi Esaki , Kenji Soda
IPC分类号： C12N1/21 , C12P13/08 , C12R1/13 , C12R1/15 , C12N1/20
CPC分类号： C12P13/08 , Y10S435/84 , Y10S435/843
摘要： A process for producing L-lysine, which comprises culturing a mutant L-lysine-producing strain belonging to the genus Brevibacterium or the genus Corynebacterium and having selenalysine resistance in a nutrient medium, producing and accumulating L-lysine in the culture broth and collecting L-lysine from the culture broth.
摘要翻译：一种生产L-赖氨酸的方法，其包括培养属于短杆菌属或棒杆菌属的突变体L-赖氨酸生产菌株并在营养培养基中具有硒酸盐耐性，在培养液中产生和积累L-赖氨酸并收集L - 赖氨酸来自培养液。

30. 发明授权

US5304476A Process for producing l-lysine by fermentation employing a microorganism resistant to acyl-lysine or methylated acyl-lysine 失效
标题翻译：通过使用对酰基赖氨酸或甲基化酰基赖氨酸有抗性的微生物的发酵来生产l-赖氨酸的方法
公开(公告)号：US5304476A
公开(公告)日：1994-04-19
申请号：US845139
申请日：1992-03-03
申请人： Manabu Yokomori , Kazuhiko Totsuka , Yoshio Kawahara , Harufumi Miwa , Tsuyoshi Osumi
发明人： Manabu Yokomori , Kazuhiko Totsuka , Yoshio Kawahara , Harufumi Miwa , Tsuyoshi Osumi
IPC分类号： C12N1/20 , C12P13/08 , C12R1/13 , C12R1/15
CPC分类号： C12P13/08 , Y10S435/84 , Y10S435/843
摘要： The present invention relates to a fermentation process using a L-lysine-producing microorganism having a resistance to an acyl-lysine, a methylated acyl-lysine or both an acyl-lysine and a methylated acyl-lysine. By the process of the present invention, L-lysine may be produced in high yields providing an improved process that reduces the cost of industrially produced L-lysine.
摘要翻译：本发明涉及使用具有对酰基赖氨酸，甲基化酰基赖氨酸或酰基赖氨酸和甲基化酰基赖氨酸两者均具有抗性的L-赖氨酸生产微生物的发酵方法。通过本发明的方法，可以高产率生产L-赖氨酸，提供降低工业生产的L-赖氨酸成本的改进方法。

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