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21. 发明申请

US20070243582A1 Process for Producing Protein a-Like Protein with Use of Brevibacillus Genus Bacterium 有权
标题翻译：使用短杆菌属细菌产生蛋白质样蛋白的方法
公开(公告)号：US20070243582A1
公开(公告)日：2007-10-18
申请号：US11630334
申请日：2005-07-01
申请人： Akihiko Kosugi , Kazuyoshi Yajima
发明人： Akihiko Kosugi , Kazuyoshi Yajima
IPC分类号： C12P21/02 , C07H21/04 , C12N1/21 , C12N15/00
CPC分类号： C07K16/1271 , C07K14/31 , C12P21/02
摘要： The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.
摘要翻译：本发明涉及用于生产蛋白质A样蛋白质的有效和经济的方法。宿主如大肠杆菌和枯草芽孢杆菌已经用于使用遗传重组技术生产蛋白质A样蛋白质，然而，它们的低生产率已成为高成本的主要原因。因此，迫切需要立即建立一种能够使用除大肠杆菌和枯草芽孢杆菌之外的重组DNA技术廉价大规模生产蛋白A样蛋白的技术。本发明提供了大量生产蛋白质A样蛋白质的方法，例如包括使重组短杆菌属细菌大量表达和分泌蛋白质并分离和收集积累的蛋白质的方法来自培养液的A样蛋白质。

22. 发明授权

US06709378B2 Supernatant liquid collector with balance weight forming function and centrifugal apparatus using the same 失效
标题翻译：具有平衡重成型功能的上清液收集器及使用其的离心装置
公开(公告)号：US06709378B2
公开(公告)日：2004-03-23
申请号：US10113864
申请日：2002-04-01
申请人： Shintaro Nishimura , Kazuyoshi Yajima , Satoshi Minoshima , Ichiro Matsunari , Hiroshi Myogan
发明人： Shintaro Nishimura , Kazuyoshi Yajima , Satoshi Minoshima , Ichiro Matsunari , Hiroshi Myogan
IPC分类号： B01D17038
CPC分类号： B01D21/262 , B01D17/00 , B01D17/0214 , B01D17/0217 , B01D17/12 , B01D2221/10
摘要： Primaiy supernatant liquid collector 1 allows blood A to separate into a primary supernatant liquid B and red blood cells by leaving the blood A stationary for a prescribed period of time, and then it discharges the primary supernatant liquid B and physiological saline W in the same amount into a first treatment container 13 and a first balance container 14, respectively. Centrifuge 61 centrifuges the above primary supernatant liquid B using the first balance container 14 as a balance weight, and a secondary supernatant liquid in the first treatment container 13 is transferred to a second treatment container 109 by take-out device 91 and at the same time the physiological saline W in the same amount as the transferred secondary supernatant liquid is transferred from the first balance container 14 to a second balance container 110.
摘要翻译： Primaiy上清液收集器1使血液A通过使血液A静止一段规定时间分离成初级上清液B和红细胞，然后以相同的量排出初级上清液B和生理盐水W 分别进入第一处理容器13和第一平衡容器14。离心机61使用第一平衡容器14作为平衡重将上述初级上清液B离心，将第一处理容器13中的第二上清液通过取出装置91转移到第二处理容器109，同时与转移的二次上清液相同量的生理盐水W从第一平衡容器14转移到第二平衡容器110。

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