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21. 发明授权

US07553626B2 Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species 有权
标题翻译：能够特异性扩增在10种细菌物种中发现的靶序列的引物组和与10种细菌物种的每个靶序列特异性杂交的探针寡核苷酸
公开(公告)号：US07553626B2
公开(公告)日：2009-06-30
申请号：US11317601
申请日：2005-12-23
申请人： Ji-young Oh , Nam Huh , Sang-hyun Peak , Sung-young Jeong
发明人： Ji-young Oh , Nam Huh , Sang-hyun Peak , Sung-young Jeong
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/689
摘要： Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.
摘要翻译：本发明提供一种引物组，其用于扩增百日盛博氏杆菌，肺炎衣原体，流感嗜血杆菌，肺炎克雷伯杆菌，嗜肺军团杆菌，卡他莫拉菌，肺炎支原体，铜绿假单胞菌，金黄色葡萄球菌和肺炎链球菌特异性的靶序列，所述引物组包括至少一种长度为10至100个核苷酸的寡核苷酸，选自寡核苷酸，每个寡核苷酸包含存在于如SEQ ID NO：1所示的序列中的至少10个连续核苷酸的片段和10至100个的至少一个寡核苷酸长度的核苷酸，选自寡核苷酸，每个寡核苷酸包含存在于如SEQ ID NO：2所示的序列中的至少10个连续核苷酸的片段; 并探测10种细菌物种的特定物种。

22. 发明申请

US20070170059A1 METHOD FOR CONTROLLING THE CONTACT ANGLE OF A DROPLET IN ELECTROWETTING AND AN APPARATUS USING THE DROPLET FORMED THEREBY 有权
标题翻译：用于控制电镀中的滴液接触角的方法和使用其形成的液滴的装置
公开(公告)号：US20070170059A1
公开(公告)日：2007-07-26
申请号：US11536319
申请日：2006-09-28
申请人： Jeong Lee , Hye Cho , Nam Huh , Sung Kim
发明人： Jeong Lee , Hye Cho , Nam Huh , Sung Kim
IPC分类号： C07K1/26
CPC分类号： B01L3/502792 , B01L2300/089 , B01L2300/161 , B01L2400/0427 , F04B19/006
摘要： A method for controlling the hydrophilic droplet using the electrowetting principle is disclosed. More specifically, the present invention provides a method for increasing the change of the contact angle in the interface that is formed between the hydrophilic droplet and the nonpolar fluid by adding a constant concentration of two-element electrolyte or three-element electrolyte to the hydrophilic droplet; and an apparatus for controlling the droplet having the change of the contact angle and velocity scope increased by the method.
摘要翻译：公开了一种使用电润湿原理控制亲水性液滴的方法。更具体地说，本发明提供了一种通过向亲水液滴中加入恒定浓度的双元素电解质或三元素电解质来增加形成在亲水性液滴和非极性流体之间的界面中的接触角变化的方法 ; 并且通过该方法增加了具有接触角和速度范围的变化的液滴的控制装置。

23. 发明申请

US20050181407A1 Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method 有权
标题翻译：用于非固定地将生物分子固定在固体基质上的方法和根据该方法制备的微阵列
公开(公告)号：US20050181407A1
公开(公告)日：2005-08-18
申请号：US11052345
申请日：2005-02-07
申请人： Nam Huh , Jong-myeon Park
发明人： Nam Huh , Jong-myeon Park
IPC分类号： C12Q1/68 , B05D3/00 , C12M1/00 , C12M1/34 , C12N15/09 , C40B60/14 , G01N33/53 , G01N33/543 , G01N33/547 , G01N37/00
CPC分类号： C12Q1/6837 , B01J2219/00387 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , C40B60/14 , G01N33/54393
摘要： Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.
摘要翻译：本发明提供一种在固体基质上非共价固定生物分子的方法，包括：提供具有第一官能团的固体基质，具有氢键赋予能力的第一官能团; 并使具有氢键接受能力的化合物和用第二官能团官能化的生物分子的混合物与底物的表面，第二官能团具有氢键供体能力反应，以将生物分子非常固定地固定在基质。

24. 发明申请

US20050064488A1 Method for detecting a target nucleic acid by using a detection probe capable of hybridizing with a tag sequence 审中-公开
标题翻译：通过使用能够与标签序列杂交的检测探针来检测靶核酸的方法
公开(公告)号：US20050064488A1
公开(公告)日：2005-03-24
申请号：US10945245
申请日：2004-09-20
申请人： Nam Huh , Sung-young Jeong , Kui-hyun Kim
发明人： Nam Huh , Sung-young Jeong , Kui-hyun Kim
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6853 , C12Q2537/143 , C12Q2537/125 , C12Q2525/161
摘要： Provided is a method for detecting a target nucleic acid, comprising amplifying a target nucleic acid from a nucleic acid sample using a set of primers comprising at least one primer having a tag sequence at its 5′ end and a target binding sequence at its 3′ end; hybridizing the resulting amplified products with detection probes labeled with a label, the detection probes being capable of specifically binding to the tag sequence; hybridizing the hybridization products with capture probes immobilized on a microarray, the capture probes being capable of specifically binding to the target nucleic acid and not to the tag sequence; and determining the results of hybridization.
摘要翻译：提供了一种用于检测靶核酸的方法，其包括使用一组引物扩增来自核酸样品的靶核酸，所述引物包含至少一个其5'末端具有标签序列的引物和其3'端的靶结合序列结束; 将得到的扩增产物与用标记物标记的检测探针杂交，检测探针能够特异性结合标签序列; 将杂交产物与固定在微阵列上的捕获探针杂交，所述捕获探针能够特异性结合靶核酸，而不能与标记序列结合; 并确定杂交结果。

25. 发明授权

US07732191B2 Hybridization system using the control of pump and valves in closed system 失效
标题翻译：混合系统使用封闭系统中的泵和阀的控制
公开(公告)号：US07732191B2
公开(公告)日：2010-06-08
申请号：US11295843
申请日：2005-12-06
申请人： Soo-suk Lee , Sung-ouk Jung , Chang-eun Yoo , Hun-joo Lee , Christopher Hansung Ko , Nam Huh
发明人： Soo-suk Lee , Sung-ouk Jung , Chang-eun Yoo , Hun-joo Lee , Christopher Hansung Ko , Nam Huh
IPC分类号： C12M1/34 , C12M1/36
CPC分类号： B01L7/00 , B01F13/0059 , B01F13/0222 , B01L3/5027 , B01L2200/0689 , B01L2300/0636 , B01L2300/14 , B01L2300/1822 , B01L2400/0487 , G01N2035/00544
摘要： Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed in the air channels; an integrated air channel to which the two air channels are connected; and an air pump disposed in the integrated air channel; and a washing and drying device including: a flow channel connected to one of the two air channels through a branched valve; a flow pump disposed in the flow channel; and a buffer inlet disposed opposite the flow channel.
摘要翻译：提供了一种用于杂交生物芯片的杂交系统，包括：至少包括一个包括用于生物芯片的支撑物的杂交室的腔室装置和具有样品入口的第一盖子; 一种搅拌装置，包括：两个连接到杂交室端部的空气通道; 设置在空气通道中的两个阀; 两个空气通道连接的集成空气通道; 以及设置在所述集成空气通道中的空气泵; 以及洗涤干燥装置，包括：流路，其通过分支阀连接到所述两个空气通道中的一个; 设置在流路中的流量泵; 以及与流动通道相对设置的缓冲器入口。

26. 发明授权

US07611889B2 Method for noncovalently immobilizing a biomolecule on a solid substrate and microarray produced according to the method 有权
标题翻译：用于非固定地将生物分子固定在固体基质上的方法和根据该方法制备的微阵列
公开(公告)号：US07611889B2
公开(公告)日：2009-11-03
申请号：US11052345
申请日：2005-02-07
申请人： Nam Huh , Jong-myeon Park
发明人： Nam Huh , Jong-myeon Park
IPC分类号： C12M1/34 , C12Q1/68 , G01N33/53 , C07K1/10
CPC分类号： C12Q1/6837 , B01J2219/00387 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , C40B60/14 , G01N33/54393
摘要： Provided is a method for noncovalently immobilizing a biomolecule on a solid substrate, including: providing a solid substrate having a first functional group attached thereto, the first functional group having a hydrogen bond donating ability; and reacting a mixture of a compound having a hydrogen bond accepting ability and a biomolecule functionalized with a second functional group, with the surface of the substrate, the second functional group having a hydrogen bond donating ability, in order to noncovalently immobilize the biomolecule on the substrate.
摘要翻译：本发明提供一种在固体基质上非共价固定生物分子的方法，包括：提供具有第一官能团的固体基质，具有氢键赋予能力的第一官能团; 并使具有氢键接受能力的化合物和用第二官能团官能化的生物分子的混合物与底物的表面，第二官能团具有氢键供体能力反应，以将生物分子非常固定地固定在基质。

27. 发明申请

US20090131649A1 METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐分离小RNA分子的方法
公开(公告)号：US20090131649A1
公开(公告)日：2009-05-21
申请号：US12110373
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H1/00
CPC分类号： C12N15/1006
摘要： Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations.
摘要翻译：公开了一种使用不同浓度的可变性盐分离从固体支持物上的较大RNA分离200个核苷酸或更少的小RNA的方法。

28. 发明申请

US20090048437A1 METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT 有权
标题翻译：使用KOSMOTROPIC盐净化RNA的方法
公开(公告)号：US20090048437A1
公开(公告)日：2009-02-19
申请号：US12110391
申请日：2008-04-28
申请人： Myo-yong LEE , Nam Huh , Joon-ho Kim
发明人： Myo-yong LEE , Nam Huh , Joon-ho Kim
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C12N15/1003 , C12N15/1006
摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
摘要翻译：本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。本发明在纯化200个或更少核苷酸的RNA中特别有效。

29. 发明申请

US20050271557A1 Microarray holding device 审中-公开
标题翻译：微阵列保持装置
公开(公告)号：US20050271557A1
公开(公告)日：2005-12-08
申请号：US11106209
申请日：2005-04-14
申请人： Hun-joo Lee , Nam Huh , Sang-hyun Paek , Chang-eun Yoo
发明人： Hun-joo Lee , Nam Huh , Sang-hyun Paek , Chang-eun Yoo
IPC分类号： G01N33/52 , B01L9/00
CPC分类号： B01L9/52 , B01J2219/00608 , B01J2219/00621 , B01J2219/00659 , B01J2219/00722 , B01L2300/0819
摘要： Provided is a microarray holding device. The microarray holding device includes: a solid substrate in the form of a plate in which an engaging-receiving portion for receiving a microarray substrate to be engaged therewith is formed, the engaging-receiving portion being composed of an opening; sidewalls to be aligned with sidewalls of the microarray substrate to be engaged with the engaging-receiving portion; and a base surface on which a top or bottom surface of the microarray substrate to be engaged with the engaging-receiving portion is to be placed, wherein the base surface is depressed from a top or bottom surface of the solid substrate such that the other surface of the microarray substrate is lower than the top or bottom surface of the solid substrate when the microarray substrate is engaged with the engaging-receiving portion.
摘要翻译：提供了一种微阵列保持装置。微阵列保持装置包括：形成板状的固体基板，其中形成用于接收与其接合的微阵列基板的接合部分，所述接合部分由开口构成; 与微阵列基板的侧壁对准以与接合部接合的侧壁; 以及要在其上放置要与所述接合部分接合的所述微阵列基板的顶表面或底表面的基面，其中所述基底表面从所述固体基底的顶表面或底表面压下，使得所述另一表面的微阵列基板与接合部接合时，低于固体基板的顶面或底面。

30. 发明申请

US20050202492A1 Microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other and a method of producing the same 审中-公开
标题翻译：具有能够结合相互最大限度分离的相同靶多核苷酸片段的探针多核苷酸点的微阵列及其制备方法
公开(公告)号：US20050202492A1
公开(公告)日：2005-09-15
申请号：US11078601
申请日：2005-03-11
申请人： Yun-sun Nam , Nam Huh , Hyeong-sop Shim , Ji-young Oh
发明人： Yun-sun Nam , Nam Huh , Hyeong-sop Shim , Ji-young Oh
IPC分类号： C12Q1/68 , A61L2/00 , C12M1/34
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00693 , B01J2219/00722 , C12Q2565/507 , C12Q2565/513
摘要： The present invention relates to a microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other, and a method of producing the same.
摘要翻译：本发明涉及具有能够结合相互最大限度分离的相同靶多核苷酸片段的探针多核苷酸斑点的微阵列及其制造方法。

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