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    • 21. 发明授权
    • Diffusion resistant lenticular element
    • 防扩散透镜元件
    • US06482769B1
    • 2002-11-19
    • US09712424
    • 2000-11-14
    • Lee W. TuttChristine M. VargasSteven Evans
    • Lee W. TuttChristine M. VargasSteven Evans
    • B41M5035
    • B41M5/38207B41M7/00B41M7/0072B41M7/009G02B3/0012G03C8/30G03C8/40G03C9/00Y10S430/146Y10S430/147
    • A diffusion resistant lenticular element comprising a support having a lenticular array thereon, the element having thereon an acidic mordanting layer containing a laser-induced, cationic dye image, the mordanting layer being on the side of said support which does not contain said lenticular array. Another embodiment of the invention relates to a process of forming a diffusion resistant lenticular element comprising: a) contacting at least one dye-donor element comprising a support having thereon a dye layer comprising an image dye in a binder having an infrared-absorbing material associated therewith, said image dye comprising a nonionic dye capable of being converted to a cationic dye by means of an acid, with a lenticular element comprising a support having a lenticular array thereon on the opposite side thereof; b) imagewise-heating the dye-donor element by means of a laser; c) transferring a dye image to the support of the lenticular element; d) contacting the dye image with an acidic mordanting layer; and e) heating the element to cause the nonionic dye to convert to a cationic dye which is mordanted in the acidic mordanting layer.
    • 一种防扩散透镜元件,包括其上具有透镜阵列的支撑体,所述元件上具有包含激光诱导的阳离子染料图像的酸性媒染层,所述媒染层位于不包含所述透镜阵列的所述支撑体侧。 本发明的另一个实施方案涉及形成防扩散透镜元件的方法,包括:a)使至少一种染料供体元件与含有红外线吸收材料的粘合剂中的至少一种染料供体元件接触, 所述图像染料包含能够通过酸转化成阳离子染料的非离子染料,透镜元件包括在其相对侧上具有透镜阵列的支撑体; b)成像加热染料供体 元素; c)将染料图像转移到双凸透镜元件的支撑体上; d)使染料图像与酸性物质接触 媒染层 和)加热该元件以使非离子性染料转变成在酸性媒染层中发光的阳离子染料。
    • 22. 发明授权
    • Ink jet printing method
    • 喷墨打印方式
    • US06481841B1
    • 2002-11-19
    • US09920105
    • 2001-08-01
    • James W. BleaseSteven EvansKaren J. Klingman
    • James W. BleaseSteven EvansKaren J. Klingman
    • B41J201
    • C09D11/40C09B5/14
    • An ink jet printing method, having the steps of: A) providing an ink jet printer that is responsive to digital data signals; B) loading the printer with an ink-receiving element having a support having thereon an ink-receiving layer, C) loading the printer with an ink jet ink set for color printing having: (a) a yellow ink having a carrier and a yellow azoaniline dye; (b) a magenta ink having a carrier and a magenta anthrapyridone dye; and (c) a cyan ink having a carrier and Direct Blue 307; and D) printing on the ink-receiving layer using the ink jet ink in response to the digital data signals.
    • 一种喷墨打印方法,具有以下步骤:A)提供响应于数字数据信号的喷墨打印机; B)用具有其上具有油墨接收层的支撑件的油墨接收元件装载打印机,C)用彩色打印用喷墨油墨装置装载打印机,其具有:(a)黄色油墨,其具有载体和黄色 偶氮苯胺染料; (b)具有载体和品红蒽吡啶酮染料的洋红色油墨; 和(c)具有载体和直接蓝307的青色油墨; 和D)响应于数字数据信号,使用喷墨油墨在油墨接受层上进行印刷。
    • 24. 发明授权
    • Dideoxynucleotide-triphosphate utilization by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
    • 来自古细菌火球菌的嗜热DNA聚合酶的双脱氧核苷酸 - 三磷酸盐利用
    • US06333183B1
    • 2001-12-25
    • US09715524
    • 2000-11-17
    • Steven EvansJoseph Anthony MamoneMaria DavisBernard A. Connolly
    • Steven EvansJoseph Anthony MamoneMaria DavisBernard A. Connolly
    • C12N912
    • C12N9/1252
    • Polymerases from the Pol I family which are able to efficiently use ddNTPs have demonstrated a much improved performance when used to sequence DNA. A number of mutations have been made to the gene coding for the Pol II family DNA polymerase from the archaeon Pyrococcus furiosus with the aim of improving ddNTP utilisation. “Rational” alterations to amino acids likely to be near the dNTP binding site (based on sequence homologies and structural information) did not yield the desired level of selectivity for ddNTPs. However, alteration at four positions (Q472, A486, L490 and Y497) gave rise to variants which incorporated ddNTPs better than the wild type, allowing sequencing reactions to be carried out at lowered ddNTP:dNTP ratios. Wild type Pfu-Pol required a ddNTP:dNTP ratio of 30:1; values of 5:1 (Q472H), 1:3 (L490Y), 1:5 (A486Y) and 5:1 (Y497A) were found with the four mutants; A486Y representing a 150-fold improvement over the wild type. A486, L490 and Y407 are on an &agr;-helix that lines the dNTP binding groove, but the side chains of the three amino acids point away from this groove; Q472 is in a loop that connects this &agr;-helix to a second long helix. None of the four amino acids can contact the dNTP directly. Therefore, the increased selectivity for ddNTPs is likely to arise from two factors: 1) Small overall changes in conformation that subtly alter the nucleotide triphosphate binding site such that ddNTPs become favoured; 2) interference with a conformational change that may be critical both for the polymerisation step and discrimination between different nucleotide triphosphates.
    • 能够有效使用ddNTP的Pol I家族的聚合酶在用于DNA序列测序时表现出非常好的表现。 已经对来自古细菌火球菌(Pyrococcus furiosus)的Pol II家族DNA聚合酶编码基因进行了许多突变,目的是改善ddNTP的利用。 可能靠近dNTP结合位点(基于序列同源性和结构信息)的氨基酸的“合理”改变没有产生对ddNTPs的期望水平的选择性。 然而,在四个位置(Q472,A486,L490和Y497)的改变产生了比野生型更好地掺入ddNTP的变体,允许在降低的ddNTP:dNTP比例下进行测序反应。 野生型Pfu-Pol需要ddNTP:dNTP比为30:1; 发现4种突变体的5:1(Q472H),1:3(L490Y),1:5(A486Y)和5:1(Y497A)的值; A486Y比野生型提高了150倍。 A486,L490和Y407位于对dNTP结合槽进行排列的α-螺旋上,但是三个氨基酸的侧链指向远离该凹槽; Q472是一个将该α-螺旋连接到第二个长螺旋的环。 四种氨基酸都不能直接与dNTP接触。 因此,对ddNTPs的选择性增加可能是由两个因素引起的:1)微小的构象改变,巧妙地改变了核苷酸三磷酸结合位点,使得ddNTPs变得有利; 2)干扰对于聚合步骤和不同核苷酸三磷酸盐之间的区别可能是关键的构象变化。