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21. 发明授权

US5776890A Hemoglobins with intersubunit disulfide bonds 失效
标题翻译：血红蛋白与亚基间二硫键
公开(公告)号：US5776890A
公开(公告)日：1998-07-07
申请号：US453666
申请日：1995-05-30
申请人： Stephen J. Hoffman , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Kiyoshi Nagai
IPC分类号： A61K38/00 , C07K14/805
CPC分类号： C07K14/805 , A61K38/00 , C07K2319/00 , Y10S514/832 , Y10S514/833
摘要： Cysteine substitution mutants of alpha and/or beta globin mutants are produced by recombinant DNA techniques and used in the construction, intracellularly or otherwise, of mutant hemoglobins in which alpha- and beta-globin like subunits are crosslinked by disulfide bonds. Solutions of these mutant hemoglobins are used as blood substitutes. Preferably, these mutant hemoglobins contain further mutations which reduce their affinity for oxygen. Hemoglobins are preferably obtained by recombinant DNA techniques. Both alpha and beta globin chains can now be readily expressed, making possible the commercial production of wholly artificial hemoglobin, whether conventional or mutant in form. Solutions of wholly artificial hemoglobins are also used as blood substitutes. Expression of the alpha globin gene was substantially improved by means of a beta globin gene "header".
摘要翻译： α和/或β球蛋白突变体的半胱氨酸取代突变体通过重组DNA技术产生并用于构建，细胞内或其它突变体血红蛋白，其中α和β-珠蛋白样亚基通过二硫键交联。这些突变体血红蛋白的溶液用作血液代用品。优选地，这些突变体血红蛋白含有进一步降低其对氧的亲和力的突变。血红蛋白优选通过重组DNA技术获得。现在可以容易地表达α和β球蛋白链，使得完全人造血红蛋白的商业生产成为可能，无论是常规的还是突变形式。完全人造血红蛋白的溶液也用作血液代用品。通过β球蛋白基因“头”，α球蛋白基因的表达显着改善。

22. 发明授权

US5373471A Semiconductor memory device having redundancy memory cells for replacing defective 失效
标题翻译：具有用于替换有缺陷的冗余存储单元的半导体存储器件
公开(公告)号：US5373471A
公开(公告)日：1994-12-13
申请号：US934332
申请日：1992-08-25
申请人： Makoto Saeki , Kiyoshi Nagai , Hisae Yamamura , Tadashi Abe , Takeshi Fukazawa
发明人： Makoto Saeki , Kiyoshi Nagai , Hisae Yamamura , Tadashi Abe , Takeshi Fukazawa
IPC分类号： G11C11/413 , G11C11/401 , G11C11/407 , G11C29/00 , G11C29/04 , G11C7/00
CPC分类号： G11C29/846
摘要： In case an address is to access a defective memory cell, a defective memory cell in a memory cell area contained in one of paired memory mats is selected in parallel with a redundant memory cell in a redundant memory cell area contained in the other memory mat. At this time of selecting the redundant main word line for selecting the redundant memory cell, there is not required the logical operation for deciding whether or not the redundant use of the access address fed from the outside is proper. For example, the redundant main word line is set to the select level on the basis of a chip select signal. As a result, the drive start timing of the redundant main word line is not delayed in the least from the drive start timing of the main word line. Thus, it is possible to prevent the event that the select drive timing of the redundant sub word line is delayed on account of the delay in the drive timing of the redundant main word line.
摘要翻译：在地址要访问有缺陷的存储单元的情况下，在包含在其中一个存储器堆中的存储单元区域中的有缺陷的存储器单元与冗余存储器单元并联地选择在另一存储器堆中所包含的冗余存储单元区域中。在选择用于选择冗余存储单元的冗余主字线时，不需要用于判断从外部馈送的访问地址的冗余使用是否合适的逻辑操作。例如，基于片选信号将冗余主字线设定为选择电平。结果，冗余主字线的驱动开始定时至少不会从主字线的驱动开始定时延迟。因此，可以防止由于冗余主字线的驱动定时的延迟而使冗余子字线的选择驱动定时被延迟的事件。

23. 发明授权

US4616902A Composite filter, observation system and filamentary display device 失效
标题翻译：复合过滤器，观察系统和丝状显示装置
公开(公告)号：US4616902A
公开(公告)日：1986-10-14
申请号：US629317
申请日：1984-07-10
申请人： Kiyoshi Nagai
发明人： Kiyoshi Nagai
IPC分类号： G02B5/20 , G02B5/22
CPC分类号： G02B5/208 , G02B5/223 , G02B5/226
摘要： A composite filter (i) which transmits starlight radiation having a wavelength of 400 up to but not including 600 nm and (ii) does not transmit radiation having a wavelength of 600 to 900 nm comprising at least one organic film filter and at least one glass filter, in which said organic film filter absorbs light radiation having a wavelength of 600-700 nm and said glass filter comprises a glass plate colored with a cobalt-containing salt which absorbs light radiation having a wavelength of 700-900 nm.
摘要翻译：传输波长为400，但不包括600nm的星光辐射的复合滤光片（i）和（ii）不透射包括至少一个有机膜滤光片和至少一个玻璃的波长为600-900nm的辐射过滤器，其中所述有机膜过滤器吸收波长为600-700nm的光辐射，并且所述玻璃过滤器包括用吸收波长为700-900nm的光辐射的含钴盐着色的玻璃板。

24. 发明授权

US4405875A Hermetically sealed flat-type piezo-electric oscillator assembly 失效
标题翻译：密封平型压电振荡器组件
公开(公告)号：US4405875A
公开(公告)日：1983-09-20
申请号：US253043
申请日：1981-04-10
申请人： Kiyoshi Nagai
发明人： Kiyoshi Nagai
IPC分类号： H03H3/02 , H03H9/10 , H01L41/08
CPC分类号： H03H9/1021
摘要： A hermetically sealed flat-type piezo-electric oscillator assembly comprising a flat-type package and a piezo-electric oscillator encased in the package.The flat-type package comprises metal frame, an insulating material portion united to the frame to form the package, and a plurality of lead wires passing through the insulating material portion to provide terminals inside of the package, and having opening for insertion of the piezo-electric oscillator element therein such that the piezo-electric oscillator element is mounted on the terminals of the lead wires. A lid is placed over the opening of the package so as to hermetically seal the package with the oscillator element encased therein. At least two side faces of the insulating material portion are, at least in part, positioned within the space defined by the metal frame to engage with an inwardly facing surface of the metal frame.
摘要翻译：一种气密密封的扁平型压电振荡器组件，包括平面型封装和封装在封装中的压电振荡器。扁平型封装包括金属框架，与框架结合以形成封装的绝缘材料部分，以及穿过绝缘材料部分提供端子的多个引线，并具有用于插入压电元件的开口其中压电振荡器元件安装在引线的端子上。将盖子放置在包装的开口上方，以便封装有封装在其中的振荡器元件的封装。绝缘材料部分的至少两个侧面至少部分地定位在由金属框架限定的空间内，以与金属框架的面向内的表面接合。

25. 发明申请

US20110086494A1 METHOD OF REMOVING HEAVY METAL IN SEMICONDUCTOR SUBSTRATE 有权
标题翻译：在半导体衬底中去除重金属的方法
公开(公告)号：US20110086494A1
公开(公告)日：2011-04-14
申请号：US12899338
申请日：2010-10-06
申请人： Noritomo Mitsugi , Masataka Hourai , Shuichi Samata , Kiyoshi Nagai , Kei Matsumoto
发明人： Noritomo Mitsugi , Masataka Hourai , Shuichi Samata , Kiyoshi Nagai , Kei Matsumoto
IPC分类号： H01L21/322
CPC分类号： H01L21/3221
摘要： To provide a method of removing a heavy metal contained in a thinned semiconductor substrate.A method of removing a heavy metal in a semiconductor substrate of the present invention comprises: attaching, to a rear surface of the semiconductor substrate, a material that lowers a potential barrier of the rear surface of the semiconductor substrate, on a front surface of which a circuit is to be formed or is formed; applying a thermal treatment to the semiconductor substrate under a condition based on a thickness and a resistivity of the semiconductor substrate; and, depositing the heavy metal in the semiconductor substrate on the rear surface.
摘要翻译：提供一种除去减薄的半导体衬底中所含的重金属的方法。本发明的半导体衬底中的去除重金属的方法包括：将半导体衬底的后表面的势垒降低到半导体衬底的背面的材料的前表面要形成或形成电路; 在基于半导体衬底的厚度和电阻率的条件下对半导体衬底进行热处理; 并且将重金属沉积在半导体衬底的后表面上。

26. 发明授权

US07767969B2 Method and apparatus for measuring spectroscopic absorbance 有权
标题翻译：用于测量光谱吸光度的方法和装置
公开(公告)号：US07767969B2
公开(公告)日：2010-08-03
申请号：US12319332
申请日：2009-01-06
申请人： Kiyoshi Nagai , Harumi Shibata , Sayaka Hamaguchi
发明人： Kiyoshi Nagai , Harumi Shibata , Sayaka Hamaguchi
IPC分类号： G01J5/06
CPC分类号： G01J3/45 , G01N21/3563 , G01N2021/3568 , G01N2021/3595
摘要： An object of the present invention is to provide a spectroscopic method and an apparatus which can measure a trace element accurately with high sensitivity. In order to achieve this object, for example, in Fourier transformation infrared spectroscopy (FT-IR), a reference spectrum and a measurement spectrum including an impurity spectrum are measured in order to obtain a differential spectrum comprising the impurity spectrum and a flat baseline, correction including a frequency shift of the reference spectrum before calculating a differential spectrum, is performed on the reference spectrum. This makes it possible to remove baseline deformation due to phonon absorbance of silicon included in the conventional differential spectrum, and to obtain an infrared absorption spectrum of the substitutional carbon with high accuracy and high sensitivity.
摘要翻译：本发明的目的是提供一种能够以高灵敏度精确地测量微量元素的分光方法和装置。为了实现该目的，例如在傅里叶变换红外光谱（FT-IR）中，测量参考光谱和包含杂质光谱的测量光谱，以获得包含杂质光谱和平坦基线的差分光谱，在参考光谱上执行包括计算差分光谱之前的参考光谱的频移的校正。这使得可以消除由常规差分光谱中包含的硅的声子吸收引起的基线变形，并以高精度和高灵敏度获得取代碳的红外吸收光谱。

27. 发明申请

US20090173884A1 Method and apparatus for measuring spectroscopic absorbance 有权
标题翻译：用于测量光谱吸光度的方法和装置
公开(公告)号：US20090173884A1
公开(公告)日：2009-07-09
申请号：US12319332
申请日：2009-01-06
申请人： Kiyoshi Nagai , Harumi Shibata , Sayaka Hamaguchi
发明人： Kiyoshi Nagai , Harumi Shibata , Sayaka Hamaguchi
IPC分类号： G01J5/02 , G01J3/45
CPC分类号： G01J3/45 , G01N21/3563 , G01N2021/3568 , G01N2021/3595
摘要： An object of the present invention is to provide a spectroscopic method and an apparatus which can measure a trace element accurately with high sensitivity. In order to achieve this object, for example, in Fourier transformation infrared spectroscopy (FT-IR), a reference spectrum and a measurement spectrum including an impurity spectrum are measured in order to obtain a differential spectrum comprising the impurity spectrum and a flat baseline, correction including a frequency shift of the reference spectrum before calculating a differential spectrum, is performed on the reference spectrum. This makes it possible to remove baseline deformation due to phonon absorbance of silicon included in the conventional differential spectrum, and to obtain an infrared absorption spectrum of the substitutional carbon with high accuracy and high sensitivity.
摘要翻译：本发明的目的是提供一种能够以高灵敏度精确地测量微量元素的分光方法和装置。为了实现该目的，例如在傅里叶变换红外光谱（FT-IR）中，测量参考光谱和包含杂质光谱的测量光谱，以获得包含杂质光谱和平坦基线的差分光谱，在参考光谱上执行包括计算差分光谱之前的参考光谱的频移的校正。这使得可以消除由常规差分光谱中包含的硅的声子吸收引起的基线变形，并以高精度和高灵敏度获得取代碳的红外吸收光谱。

28. 发明授权

US06828125B1 DNA encoding fused di-alpha globins and use thereof 失效
标题翻译：编码融合二-α球蛋白的DNA及其用途
公开(公告)号：US06828125B1
公开(公告)日：2004-12-07
申请号：US08453485
申请日：1995-05-30
申请人： Stephen J. Hoffman , Douglas L. Looker , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Douglas L. Looker , Kiyoshi Nagai
IPC分类号： C12P2106
CPC分类号： C07K14/805 , A61K38/00 , A61K47/6445 , C07K2319/00 , C12N15/81
摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.
摘要翻译：在本文中，血红蛋白的α亚基本质上被形成为与β-亚基非共价结合的分离的多肽链，这里以新分子二-α球蛋白的形式提供，单链多肽链通过将两个α亚基连接而定义直接通过肽键或通过柔性氨基酸或肽接头间接。 Di-α珠蛋白可以在体内或体外与β球蛋白和血红素组合以形成血红蛋白。 Di-α球蛋白通过重组DNA技术表达。可以类似地获得二β-珠蛋白。

29. 发明授权

US6010883A Recombinant fusion proteins 失效
标题翻译：重组融合蛋白
公开(公告)号：US6010883A
公开(公告)日：2000-01-04
申请号：US854749
申请日：1992-03-19
申请人： Kiyoshi Nagai , Hans Christian Th.o slashed.gersen
发明人： Kiyoshi Nagai , Hans Christian Th.o slashed.gersen
IPC分类号： C12N15/09 , A61K35/74 , A61K38/00 , C07H21/04 , C07K1/22 , C07K14/00 , C07K14/195 , C07K14/41 , C07K14/47 , C07K14/575 , C07K14/585 , C07K14/805 , C07K19/00 , C12N1/20 , C12N1/21 , C12N9/00 , C12N9/10 , C12N15/00 , C12N15/62 , C12P21/00 , C12P21/02 , C12R1/125 , C12R1/19 , C12N15/12 , C12N15/13 , C12N15/14 , C12N15/74
CPC分类号： C12N9/1033 , C07K14/4716 , C07K14/585 , C07K14/805 , C12N15/62 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/61 , C07K2319/75
摘要： This invention provides a DNA sequence coding for a cleavage site which is specifically cleaved by blood coagulation Factor Xa, a vector containing such a sequence, and a host organism transformed with such a vector. Preferably, in the vector, the Factor Xa cleavage site coding sequence is fused at one end to a product and at its other end to an ATG codon or a sequence coding for at least part of a host protein.This invention also provides a process, for the production of a desired protein or peptide product in native form, comprising:transforming a host organism with a vector as described above;expressing the desired protein or peptide product as a fusion protein comprising the desired protein or peptide product fused to a Factor Xa cleavage site; anda cleaving the fusion protein with Factor Xa to yield the foreign gene product in native form.
摘要翻译：本发明提供编码由凝血因子Xa特异性切割的切割位点的DNA序列，含有该序列的载体和用该载体转化的宿主生物体。优选地，在载体中，因子Xa切割位点编码序列在一端与产物融合，而在另一端与ATG密码子或编码至少部分宿主蛋白的序列融合。本发明还提供了一种用于生产天然形式的所需蛋白质或肽产物的方法，其包括：用如上所述的载体转化宿主生物; 表达所需的蛋白质或肽产物作为融合蛋白，其包含与因子Xa切割位点融合的所需蛋白质或肽产物; 并用因子Xa切割融合蛋白以产生天然形式的外源基因产物。

30. 发明授权

US5844088A Hemoglobin-like protein comprising genetically fused globin-like polypeptides 失效
公开(公告)号：US5844088A
公开(公告)日：1998-12-01
申请号：US444991
申请日：1995-05-19
申请人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
发明人： Stephen J. Hoffman , Douglas L. Looker , Mary S. Rosendahl , Gary L. Stetler , Michael Wagenbach , David C. Anderson , Antony James Mathews , Kiyoshi Nagai
IPC分类号： A61K38/16 , A61K38/00 , A61K47/48 , A61P7/08 , C07K20060101 , C07K1/113 , C07K14/805 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/91
CPC分类号： C07K14/805 , A61K47/48307 , C12N15/81 , A61K38/00 , C07K2319/00 , C07K2319/50 , C07K2319/92
摘要： The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained. We further describe the production of tetrameric human hemoglobin and di-alpha/beta.sub.2 hemoglobin in the yeast Saccharomyces cerevisiae. The synthesis of the protein is directed by a synthetic promotor consisting of two functional parts, an upstream activator sequence (UAS) that confers inducible transcription by galactose from a consensus yeast transcriptional initiation site. The expression construct is designed such that translation is expected to initiate at the same position as the human wild-type genes for .alpha.- and .beta.-globin. Three different types of expression vectors have been used: (1) .alpha.-globin and .beta.-globin contained on two separate plasmids (pGS4688 and pGS4988) in a diploid yeast strain; (2) .alpha.-globin and .beta.-globin each contained on a single plasmid (pGS289 and pGS389) and expressed in either haploid or diploid strains; and (3) di-alpha-globin and beta globin contained on a single plasmid (pGS 3089)and expressed in haploid strains. Finally, we describe the co-expression of alpha and beta globin chains. The chains are folded together and combined intracellularly with heme to form active tetrameric hemoglobin. The hemoglobin may be recovered from the cells' soluble fraction. The invention thus obviates the need to express alpha and beta globin separately, solubilize, renature and purify them, and combine them in vitro with heme to obtain an artificial hemoglobin. By way of comparison, the separately expressed beta globin known in the art is deposited in inclusion bodies. Polycistronic co-expression of alpha and beta globins is particularly preferred.

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