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21. 发明申请

US20170137794A1 A Method for Modifying A Lipase 审中-公开
公开(公告)号：US20170137794A1
公开(公告)日：2017-05-18
申请号：US15310159
申请日：2015-05-26
申请人： Novozymes A/S
发明人： Anna Verena Reiser , Vikram Kjoeller Bhatia , Kim Borch , Christian I. Joergensen , Birgitte Andersen , Lone Baunsgaard
IPC分类号： C12N9/20 , C11D3/386 , C12N9/50
CPC分类号： C12N9/20 , C11D3/386 , C11D3/38627 , C11D3/38681 , C12N9/50 , C12Y301/01003 , C12Y304/21064
摘要： The present invention relates to a method for modifying one or more characteristics of a lipase, comprising the step of associating a peptide to the lipase, wherein the peptide has at least 50% sequence identity with the amino acid sequence of at least one major lipase contact zone of the propeptide of said lipase.

22. 发明授权

US09631164B2 Polypeptides with lipase activity and polynucleotides encoding same 有权
公开(公告)号：US09631164B2
公开(公告)日：2017-04-25
申请号：US14770006
申请日：2014-03-19
申请人： Novozymes A/S
发明人： Marc Dominique Morant , Anna Verana Reiser , Carsten Hoerslev Hansen , Kim Borch , Lone Baunsgaard , Vikram Kjoeller Bhatia
IPC分类号： C12N9/20 , C12Q1/34 , C12Q1/44 , C07H21/04 , C11D3/00 , C11D7/44 , C12S11/00 , C11D3/386 , C12P13/00
CPC分类号： C11D3/38627 , C11D3/38681 , C12N9/20 , C12P13/008
摘要： The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

23. 发明申请

US20170096620A1 Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same 审中-公开
公开(公告)号：US20170096620A1
公开(公告)日：2017-04-06
申请号：US15128010
申请日：2015-03-27
申请人： Novozymes A/S
发明人： Tianqi Sun , Ming Li , Kim Borch , Allan Noergaard , Jesper Brask , Marianne Linde Damstrup , Hanna Maria Lilbaek
IPC分类号： C11B3/00 , C12N15/52 , C12N9/20
CPC分类号： C11B3/003 , C07H21/00 , C12N9/20 , C12N15/52 , C12Y301/04003
摘要： The present invention provides polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. Furthermore, the present invention provides a method of reducing the phospholipid content in an oil composition using the polypeptide having phospholipase C activity as well as other relevant uses of the polypeptide.

24. 发明申请

US20170096619A1 Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same 审中-公开
公开(公告)号：US20170096619A1
公开(公告)日：2017-04-06
申请号：US15126098
申请日：2015-03-19
申请人： Novozymes A/S
发明人： Dorotea Raventos Segura , Robert Piotr Olinski , Kim Borch , Allan Noergaard , Jesper Brask , Rune Falkenberg Haller , Marianne Linde Damstrup
IPC分类号： C11B3/00 , C12N9/20
CPC分类号： C11B3/003 , C12N9/16 , C12N9/20 , C12P7/6445 , C12Y301/04003 , C12Y301/04011
摘要： The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

25. 发明申请

US20170015982A1 POLYPEPTIDES WITH LIPASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有脂酶活性的多肽和编码其的多核苷酸
公开(公告)号：US20170015982A1
公开(公告)日：2017-01-19
申请号：US15121186
申请日：2015-03-10
申请人： NOVOZYMES A/S
发明人： Ming Li , tianqi Sun , Xianshi Jiang , Lone Baunsgaard , Carsten Hoerslev Hansen , Kim Borch , Han Bucong
IPC分类号： C12N9/20 , C11D11/00 , C11D3/386
CPC分类号： C12N9/20 , C11D3/38627 , C11D3/38681 , C11D11/0017 , C12Y301/01
摘要： Provided are isolated polypeptides with lipase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the polypeptides.
摘要翻译：提供具有脂肪酶活性的分离的多肽和编码多肽的多核苷酸。还提供了包含多核苷酸的核酸构建体，载体和宿主细胞，以及使用多肽的方法。

26. 发明申请

US20160160196A1 Polypeptides with Lipase Activity And Polynucleotides Encoding Same 审中-公开
标题翻译：具有脂肪酶活性的多肽和编码相同的多核苷酸
公开(公告)号：US20160160196A1
公开(公告)日：2016-06-09
申请号：US14902679
申请日：2014-07-08
申请人： NOVOZYMES A/S
发明人： Marc Dominique Morant , Anna Verena Reiser , Carsten Hoerslev Hansen , Kim Borch , Lone Baunsgaard , Vikram Kjoeller Bhatia
IPC分类号： C12N9/20 , C11D3/386
CPC分类号： C12N9/20 , C11D3/38627 , C11D3/38681 , C12Y301/01 , C12Y301/01003 , D06L4/40
摘要： The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 1 or the full-length complement of (i); (c)a polypeptide encoded by a polynucleotide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 1; (d) a polypeptide which is a variant of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
摘要翻译：本发明涉及具有脂肪酶活性的分离的多肽，其选自：（a）多肽，其具有至少80％，至少85％，至少90％，至少91％，至少92％，在与SEQ ID NO：2的至少93％，至少94％，至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （b）由多核苷酸编码的多肽，其在低严格条件，中等严格条件，中等严格条件，高严格条件或非常高严格条件下与（i）SEQ ID NO：1或全长补体的（i）; （c）由多核苷酸编码的多肽，所述多肽具有至少80％，至少85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％与SEQ ID NO：1的至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （d）在一个或多个（例如多个）位置上包含取代，缺失和/或插入的SEQ ID NO：2的变体的多肽; 和（e）作为（a），（b），（c）或（d）的多肽的片段的多肽。本发明还涉及编码多肽的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。

27. 发明申请

US20160143309A1 POLYPEPTIDES HAVING PHOSPHOLIPASE C ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：具有磷脂酶C活性的多肽和编码其的多核苷酸
公开(公告)号：US20160143309A1
公开(公告)日：2016-05-26
申请号：US15011920
申请日：2016-02-01
申请人： Novozymes A/S
发明人： Lan Tang , Ye Liu , Kim Borch , Jesper Brask
IPC分类号： A23D9/04 , C12N9/16
CPC分类号： A23D9/04 , C11B3/003 , C12N9/16 , C12N9/20 , C12Y301/04003
摘要： The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译：本发明涉及具有磷脂酶C活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

28. 发明申请

US20150368627A1 Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same 审中-公开
标题翻译：具有磷脂酶C活性的多肽和编码相同的多核苷酸
公开(公告)号：US20150368627A1
公开(公告)日：2015-12-24
申请号：US14649084
申请日：2013-12-11
申请人： NOVOZYMES A/S
发明人： Tianqi Sun , Ye Liu , Ming Li , Kim Borch
IPC分类号： C12N9/16 , C12N15/82
CPC分类号： C12N9/16 , A23D9/013 , C11B3/003 , C11B3/04 , C12N15/8247 , C12Y301/04003
摘要： Provided are isolated polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译：提供具有磷脂酶C活性的分离的多肽和编码多肽的多核苷酸。还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

29. 发明申请

US20130337111A1 Screening Method 审中-公开
公开(公告)号：US20130337111A1
公开(公告)日：2013-12-19
申请号：US13964166
申请日：2013-08-12
申请人： Novozymes A/S
发明人： Christel Thea Jorgensen , Luise Erlandsen , Lone Dybdal Nilsson , Kim Borch , Jesper Vind
IPC分类号： A21D8/04 , C12Q1/44
CPC分类号： A21D8/042 , C12Q1/44 , G01N2333/918
摘要： Lipolytic enzymes which improve the properties of dough or baked products generally have a high activity towards lipids which are capable of forming a hexagonal phase, and a screening method was developed on this basis. The improved properties may include a larger loaf volume, an improved shape factor, an improved crumb structure, reduced dough stickiness, improved dough stability and/or improved tolerance towards extended proofing.

30. 发明公开

US20240124805A1 LIPASE WITH LOW MALODOR GENERATION 审中-公开
公开(公告)号：US20240124805A1
公开(公告)日：2024-04-18
申请号：US18262051
申请日：2022-01-27
申请人： Novozymes A/S
发明人： Iben Damager , Mary Ann Stringer , Kim Borch
IPC分类号： C11D3/386
CPC分类号： C11D3/38627 , C11D2111/12
摘要： The present invention concerns detergent compositions comprising a lipase with low malodor generation during lipid stain removal.

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