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    • 8. 发明申请
    • Polypeptides with Lipase Activity And Polynucleotides Encoding Same
    • 具有脂肪酶活性的多肽和编码相同的多核苷酸
    • US20160160196A1
    • 2016-06-09
    • US14902679
    • 2014-07-08
    • NOVOZYMES A/S
    • Marc Dominique MorantAnna Verena ReiserCarsten Hoerslev HansenKim BorchLone BaunsgaardVikram Kjoeller Bhatia
    • C12N9/20C11D3/386
    • C12N9/20C11D3/38627C11D3/38681C12Y301/01C12Y301/01003D06L4/40
    • The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 1 or the full-length complement of (i); (c)a polypeptide encoded by a polynucleotide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 1; (d) a polypeptide which is a variant of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
    • 本发明涉及具有脂肪酶活性的分离的多肽,其选自:(a)多肽,其具有至少80%,至少85%,至少90%,至少91%,至少92%,在 与SEQ ID NO:2的至少93%,至少94%,至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性; (b)由多核苷酸编码的多肽,其在低严格条件,中等严格条件,中等严格条件,高严格条件或非常高严格条件下与(i)SEQ ID NO:1或全长补体 的(i); (c)由多核苷酸编码的多肽,所述多肽具有至少80%,至少85%,至少90%,至少91%,至少92%,至少93%,至少94%,至少95% 与SEQ ID NO:1的至少96%,至少97%,至少98%,至少99%或100%的序列同一性; (d)在一个或多个(例如多个)位置上包含取代,缺失和/或插入的SEQ ID NO:2的变体的多肽; 和(e)作为(a),(b),(c)或(d)的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。