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21. 发明申请

US20100162433A1 Plants with improved nitrogen utilization and stress tolerance 审中-公开
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US20100162433A1
公开(公告)日：2010-06-24
申请号：US11977768
申请日：2007-10-26
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
IPC分类号： A01H5/00 , C12N15/82 , C12N15/74 , C07K16/00 , C12N1/21 , C12N5/10
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。在各种实施方案中，载体构建体包含一个或多个选自SEQ ID NO： 32,34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

22. 发明申请

US20090004698A1 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
标题翻译：提高木糖醇降解酶活性的方法
公开(公告)号：US20090004698A1
公开(公告)日：2009-01-01
申请号：US12145092
申请日：2008-06-24
申请人： Brian Vande Berg , Brian Carr , Nicholas B. Duck
发明人： Brian Vande Berg , Brian Carr , Nicholas B. Duck
IPC分类号： C12P19/00 , C12N9/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译：提供水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理接触。还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。还提供了用于从植物材料释放诸如酶，药物或营养品的物质的方法。这些方法比目前的方法更有效，更经济，毒性更低。

23. 发明申请

US20070218530A1 METHODS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSE 审中-公开
标题翻译：纤维素酶水解的方法
公开(公告)号：US20070218530A1
公开(公告)日：2007-09-20
申请号：US11748727
申请日：2007-05-15
申请人： Nicholas Duck , Brian Carr , Michael Koziel , Nadine Carozzi , Brian Vande Berg
发明人： Nicholas Duck , Brian Carr , Michael Koziel , Nadine Carozzi , Brian Vande Berg
IPC分类号： C12P19/04 , C12P19/02 , C12P19/12
CPC分类号： C12P19/14
摘要： Compositions and methods for biomass conversion are provided. Compositions comprise novel enzyme mixtures that can be used directly on lignocellulose substrate. Methods involve converting lignocellulosic biomass to free sugars and small oligosaccharides with enzymes that break down lignocellulose. Novel combinations of enzymes are provided that provide a synergistic release of sugars from plant biomass. Also provided are methods to identify enzymes, strains producing enzymes, or genes that encode enzymes capable of degrading lignocellulosic material to generate sugars.
摘要翻译：提供了生物质转化的组合物和方法。组合物包含可直接用于木质纤维素底物的新型酶混合物。方法涉及将木质纤维素生物质转化为游离糖和小分子糖，以分解木质纤维素酶。提供了新的酶组合，其提供来自植物生物质的糖的协同释放。还提供了鉴定酶，产生酶的菌株或编码能够降解木质纤维素材料以产生糖的酶的基因的方法。

24. 发明授权

US07834249B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US07834249B2
公开(公告)日：2010-11-16
申请号：US11943801
申请日：2007-11-21
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N15/52 , C12N15/82 , C12N1/21
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

25. 发明申请

US20090004692A1 METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES 审中-公开
公开(公告)号：US20090004692A1
公开(公告)日：2009-01-01
申请号：US12144993
申请日：2008-06-24
申请人： Brian Vande Berg , Jill Hinson , Paresma R. Patel , Brian Carr , Nicholas B. Duck
发明人： Brian Vande Berg , Jill Hinson , Paresma R. Patel , Brian Carr , Nicholas B. Duck
IPC分类号： C12P21/06 , C12P19/04 , C12P13/04 , C12P7/02 , C12P7/62 , C08B1/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.

26. 发明申请

US20070300325A1 GRG32: A NOVEL EPSP SYNTHASE GENE CONFERRING HERBICIDE RESISTANCE 有权
标题翻译： GRG32：一种新的EPSP合成酶基因，其抗除草剂抗性
公开(公告)号：US20070300325A1
公开(公告)日：2007-12-27
申请号：US11769255
申请日：2007-06-27
申请人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
发明人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。本发明的核苷酸序列可以用于DNA构建体或表达盒中用于转化和生物体，包括微生物和植物。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1或14所示的核苷酸序列，编码SEQ ID NO：2所示氨基酸序列的核苷酸序列，作为登录号NRRL B-30931的细菌宿主，以及其变体和片段。

27. 发明申请

US20070169218A1 EPSP synthase domains conferring glyphosate resistance 审中-公开
标题翻译：赋予草甘膦抗性的EPSP合酶结构域
公开(公告)号：US20070169218A1
公开(公告)日：2007-07-19
申请号：US11651752
申请日：2007-01-10
申请人： Brian Carr , Philip Hammer , Todd Hinson , Brian Vande Berg
发明人： Brian Carr , Philip Hammer , Todd Hinson , Brian Vande Berg
IPC分类号： A01H1/00 , C07H21/04 , C12N9/10 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括新型EPSP合成酶和编码这种酶的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。新型蛋白质包含选自本文提供的结构域的至少一个序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合成酶。

28. 发明授权

US09994863B2 Glyphosate tolerant corn event VCO-O1981-5 and kit and method for detecting the same 有权
公开(公告)号：US09994863B2
公开(公告)日：2018-06-12
申请号：US14235219
申请日：2012-07-26
申请人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
发明人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
IPC分类号： A01H5/10 , C12N15/82 , C12N9/10 , C12Q1/68
CPC分类号： C12N15/8275 , A01H5/10 , C12N9/1092 , C12Q1/6895 , C12Y205/01019 , Y10T436/143333
摘要： The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds.The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.

29. 发明申请

US20140325697A1 Glyphosate tolerant corn event VCO-O1981-5 and kit and method for detecting the same 有权
标题翻译：草甘膦耐受性玉米事件VCO-O1981-5及其检测方法
公开(公告)号：US20140325697A1
公开(公告)日：2014-10-30
申请号：US14235219
申请日：2012-07-26
申请人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
发明人： Lori Artim Artim Mann , Vadim Beilinson , Nadine Carozzi , Rebekah Deter , Brian Vande Berg , Alain Toppan , Laurent Beuf , Georges Freyssinet
IPC分类号： C12N15/82 , C12Q1/68
CPC分类号： C12N15/8275 , A01H5/10 , C12N9/1092 , C12Q1/6895 , C12Y205/01019 , Y10T436/143333
摘要： The present invention relates to the field of plant transformation with genes conferring tolerance to glyphosate. The invention particularly relates to a maize (corn) plant transformed with a gene encoding an EPSPS providing the plant tolerance to an application of glyphosate under conditions where this herbicide is effective in killing weeds.The invention particularly concerns an elite transformation event VCO-Ø1981-5 comprising the gene construct and means, kits and methods for detecting the presence of the said elite event.
摘要翻译：本发明涉及具有赋予对草甘膦耐受性的基因的植物转化领域。本发明特别涉及用编码EPSPS的基因转化的玉米（玉米）植物，其在该除草剂有效杀死杂草的条件下提供植物对施用草甘膦的耐受性。本发明特别涉及包含基因构建的精英变换事件VCO-Ø1981-5以及用于检测所述精英事件的存在的手段，工具箱和方法。

30. 发明申请

US20120144520A1 Plants with Improved Nitrogen Utilization and Stress Tolerance 审中-公开
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US20120144520A1
公开(公告)日：2012-06-07
申请号：US13309308
申请日：2011-12-01
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N15/82 , A01H5/10
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。在各种实施方案中，载体构建体包含选自SEQ ID NO： 34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

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