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21. 发明授权

US07432102B2 Transformants producing substance pf1022 derivatives, methods for producing the same, and novel biosynthesis genes 失效
标题翻译：产生物质pf1022衍生物的转化体，其制备方法和新的生物合成基因
公开(公告)号：US07432102B2
公开(公告)日：2008-10-07
申请号：US10472587
申请日：2002-03-22
申请人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
发明人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
IPC分类号： C12N1/00 , C12P1/00 , C12P17/00 , C12P15/00 , C12P13/00 , C12P7/00 , C12P7/24 , C12P1/02
CPC分类号： C12P17/14 , C12N15/52
摘要： An objective of the present invention is to provide a method for producing substance PF1022 derivatives, in particular PF1022-220 and PF1022-260, by a direct fermentation method, and a transformant to be used for this method. According to the present invention, there is provided a transformant producing substance FF1022 derivatives, which can be obtained by introducing a genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a papA gene encoding 4-amino-4-deoxychorismate synthase. which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 2; a papB gene encoding 4-amino-4-deoxyclaismate mutase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 4; and a papC gene encoding 4-amino-4-deoxyprepbenate dehydrogenase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 6, into a phenylalanine auxotrophic host induced from an organism that produces a substance PF1022. According to the present invention, there is also provided a process of producing substance PF1022 derivative, comprising steps of culturing the above-mentioned transformant and collecting the substance PF1022 derivatives.
摘要翻译：本发明的目的是提供通过直接发酵方法生产物质PF1022衍生物，特别是PF1022-220和PF1022-260的方法，以及用于该方法的转化体。根据本发明，提供了转化体生产物质FF1022衍生物，其可以通过将参与生物合成途径的基因从氯磺酸引入对氨基苯基丙酮酸而获得，包括编码4-氨基-4-脱氧胸苷酸的papA基因合酶。该基因包含编码SEQ ID NO：2的氨基酸序列的DNA序列; 编码4-氨基-4-脱氧胆酸酯变位酶的papB基因，该基因包含编码SEQ ID NO：4的氨基酸序列的DNA序列; 和编码4-氨基-4-脱氧降磷酸脱氢酶的papC基因，该基因包含编码SEQ ID NO：6的氨基酸序列的DNA序列，由产生PF1022的生物体诱导的苯丙氨酸营养缺陷型宿主中。根据本发明，还提供了生产物质PF1022衍生物的方法，包括培养上述转化体并收集物质PF1022衍生物的步骤。

22. 发明申请

US20070108401A1 Actuator for controlling brake hydraulic pressure and solenoid valve thereof 有权
标题翻译：用于控制制动液压的致动器及其电磁阀
公开(公告)号：US20070108401A1
公开(公告)日：2007-05-17
申请号：US11589896
申请日：2006-10-31
申请人： Takayuki Shibata , Koji Yanai , Toru Fujita , Tomoaki Kawabata
发明人： Takayuki Shibata , Koji Yanai , Toru Fujita , Tomoaki Kawabata
IPC分类号： F16K31/02 , B60T8/36
CPC分类号： B60T8/363 , B60T8/368 , F16K27/003 , F16K31/0675 , Y10S251/903
摘要： A solenoid valve for use in an actuator for controlling brake hydraulic pressure includes a valve housing, a movable core having an integral valve rod and axially slidably received in the valve housing, and a valve body configured to be moved into and out of contact with a valve seat when the valve body moves. The solenoid valve further includes a yoke having a U-shaped section. Even with such a U-shaped yoke, it is possible to form a magnetic path. Compared to tubular yokes, it is possible to reduce its width and thus the width of the solenoid valve by an amount equal to the thickness of tubular yokes. Thus, by arranging such solenoid valves in an actuator for controlling brake hydraulic pressure such that the width directions of the respective solenoid valves coincide with the height direction of the actuator, in which it is required to reduce the size of the actuator, it is possible to reduce its size in this direction. The flanges of the yoke have first ends that extend substantially along the outer peripheral edges of the coil winding to minimize the length of the flanges and thus the length of the solenoid valve.
摘要翻译：一种用于控制制动液压的致动器的电磁阀包括阀壳体，具有一体式阀杆并可轴向可滑动地容纳在阀壳体中的可动芯体，以及被配置成移动进入和脱离接触的阀体阀体运动时阀座。电磁阀还包括具有U形截面的轭。即使使用这种U形轭，也可以形成磁路。与管状轭铁相比，可以将电磁阀的宽度和宽度减小等于管状轭铁的厚度。因此，通过将这种电磁阀设置在用于控制制动液压的致动器中，使得各个电磁阀的宽度方向与致动器的高度方向重合，在该方向上需要减小致动器的尺寸，以减小其在这个方向的大小。轭的凸缘具有基本上沿着线圈绕组的外周缘延伸的第一端，以最小化凸缘的长度并因此使电磁阀的长度最小化。

23. 发明申请

US20070099265A1 Surfactant tolerant cellulase and method for modification thereof 有权
标题翻译：表面活性剂耐受性纤维素酶及其修饰方法
公开(公告)号：US20070099265A1
公开(公告)日：2007-05-03
申请号：US10582002
申请日：2004-12-07
申请人： Manabu Watanabe , Koji Yanai , Yumiko Tsuyuki
发明人： Manabu Watanabe , Koji Yanai , Yumiko Tsuyuki
IPC分类号： C12P21/06 , C12N9/42 , C07H21/04 , C12N15/74 , C12N1/16
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.
摘要翻译：一种抑制表面活性剂存在下内切葡聚糖酶活性降低的方法，其特征在于将具有N-端是除焦谷氨酸以外的氨基酸的具有内切葡聚糖酶活性的蛋白质修饰为具有N末端的蛋白质焦谷氨酸。此外，具有内切葡聚糖酶活性的修饰蛋白质，其中N-末端氨基酸通过氨基酸修饰转化为焦谷氨酸，编码该蛋白质的多核苷酸，包含该多核苷酸的表达载体，用表达载体转化的宿主细胞，以及公开了通过培养宿主细胞产生蛋白质的方法。

24. 发明申请

US20060035361A1 Novel cellulase tolerant to surfactants 有权
标题翻译：新型纤维素酶耐受表面活性剂
公开(公告)号：US20060035361A1
公开(公告)日：2006-02-16
申请号：US10533310
申请日：2003-10-31
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N9/24
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素酶，其中纤维素酶NCE5的氨基酸序列中的第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素酶的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有该纤维素酶的纤维素酶制剂和洗涤组合物。本发明的纤维素酶对表面活性剂具有抗性，即使在碱性条件下也能保持高活性。

25. 发明授权

US08440400B2 Process for amplifying DNA in cells 有权
标题翻译：在细胞中扩增DNA的方法
公开(公告)号：US08440400B2
公开(公告)日：2013-05-14
申请号：US12996108
申请日：2009-06-05
申请人： Takeshi Murakami , Naomi Sumida , Koji Yanai
发明人： Takeshi Murakami , Naomi Sumida , Koji Yanai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12N15/63 , C12Q1/6844 , C12Q2545/101 , C12Q2531/10
摘要： The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
摘要翻译：本发明涉及一种有效扩增巨DNA的方法。更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i）编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代，在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

26. 发明申请

US20110171692A1 PROCESS FOR AMPLIFYING DNA IN CELLS 有权
标题翻译：在细胞中放大DNA的方法
公开(公告)号：US20110171692A1
公开(公告)日：2011-07-14
申请号：US12996108
申请日：2009-06-05
申请人： Takeshi Murakami , Naomi Sumida , Koji Yanai
发明人： Takeshi Murakami , Naomi Sumida , Koji Yanai
IPC分类号： C12P19/34 , C12N1/21 , C12N1/00 , C07K14/36 , C12N15/63
CPC分类号： C12Q1/686 , C12N15/63 , C12Q1/6844 , C12Q2545/101 , C12Q2531/10
摘要： The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
摘要翻译：本发明涉及一种有效扩增巨DNA的方法。更具体地说，本发明涉及一种用于扩增细胞中DNA的方法，包括在选自以下（i），（ii）和（iii）的DNA存在下扩增作为扩增靶的DNA：（i）编码选自以下1），2）和3）的蛋白质的DNA：1）由SEQ ID NO：1的氨基酸序列组成的蛋白质，2）包含氨基酸序列的蛋白质，所述氨基酸序列具有缺失，取代，在SEQ ID NO：1的氨基酸序列中插入或添加一个或多个氨基酸，以及3）包含与SEQ ID NO：1的氨基酸序列具有90％以上相同性的氨基酸序列的蛋白质。 1，（ii）由SEQ ID NO：2的核苷酸序列组成的DNA，和（iii）在严格条件下与SEQ ID NO：2的核苷酸序列杂交的DNA。

27. 发明申请

US20080227315A1 Terminal and connecting structure between terminal and board 审中-公开
标题翻译：终端和终端之间的终端和连接结构
公开(公告)号：US20080227315A1
公开(公告)日：2008-09-18
申请号：US12076171
申请日：2008-03-14
申请人： Shigeki Banno , Takahiro Naganuma , Koji Yanai
发明人： Shigeki Banno , Takahiro Naganuma , Koji Yanai
IPC分类号： H01R12/00
CPC分类号： H01R12/58 , H05K3/308
摘要： A terminal adapted to be inserted into a through-hole of a board from an insertion end and electrically connected to the board, includes a body portion in a cylindrical shape, a first retaining portion outwardly extending from the body portion so as to be elastically deformable in a perpendicular direction relative to an axial direction of the body portion and contacting a first surface of the board or a boundary portion between the first surface and the through-hole, and a second retaining portion positioned at a counter-insertion end side relative to the first retaining portion, outwardly protruding from the body portion and contacting a second surface, which is an opposite surface of the first surface, or a boundary portion between the second surface and the through-hole.
摘要翻译：适于从插入端插入板的通孔并电连接到板的端子包括圆柱形的主体部分，从主体部分向外延伸以便可弹性变形的第一保持部分在相对于所述主体部的轴向的垂直方向上，并且接触所述基板的第一表面或所述第一表面与所述通孔之间的边界部分，以及相对于所述通孔位于相反插入端侧的第二保持部第一保持部分，从主体部分向外突出并且接触作为第一表面的相对表面的第二表面或第二表面和通孔之间的边界部分。

28. 发明申请

US20080199938A1 Novel Cellulase Resistant to Surfactant 有权
标题翻译：新型纤维素酶耐表面活性剂
公开(公告)号：US20080199938A1
公开(公告)日：2008-08-21
申请号：US12101683
申请日：2008-04-11
申请人： Kaoru OKAKURA , Koji Yanai
发明人： Kaoru OKAKURA , Koji Yanai
IPC分类号： C12N9/42 , C07H21/04 , C12N15/00 , C12N1/00 , D06M16/00 , D21C1/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素，其中纤维素NCE5的氨基酸序列中第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有纤维素的纤维素制剂和洗涤组合物。本发明的纤维素耐表面活性剂，即使在碱性条件下也能保持高活性。

29. 发明申请

US20080165510A1 Board-housing case and fluid pressure control device including thereof 失效
标题翻译：板壳体及其压力控制装置
公开(公告)号：US20080165510A1
公开(公告)日：2008-07-10
申请号：US12003873
申请日：2008-01-03
申请人： Kosei Nishimoto , Koji Yanai , Takahiro Naganuma
发明人： Kosei Nishimoto , Koji Yanai , Takahiro Naganuma
IPC分类号： H05K5/00 , B60T8/40
CPC分类号： H05K5/0047 , B60T8/3675 , H05K1/0263 , H05K3/32 , H05K3/3447 , H05K7/142 , H05K2201/1003 , H05K2201/10295 , H05K2201/10424 , H05K2201/10962
摘要： A resin case main body includes a connector housing, a component housing, and a joint portion. A busbar includes a reinforcement plate portion which is orthogonal to a plane of a board and is parallel to an alignment direction of the connector housing, the joint portion, and the component housing. The reinforcement plate portion is formed by insert molding in the joint portion.
摘要翻译：树脂壳体主体包括连接器壳体，部件壳体和接头部分。母线包括加强板部分，其与板的平面正交并且平行于连接器壳体，接合部分和部件壳体的对准方向。加强板部分通过在接头部分中嵌入成型而形成。

30. 发明授权

US07285404B1 Cyclic depsipeptide synthetase and method for recombinant production 失效
标题翻译：循环depipipeptide合成酶和重组生产方法
公开(公告)号：US07285404B1
公开(公告)日：2007-10-23
申请号：US10070387
申请日：2000-09-07
申请人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
发明人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
IPC分类号： C12N9/00
CPC分类号： C12N9/00 , C12P21/00
摘要： The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.
摘要翻译：本发明提供合成环状缩肽，特别是物质1022及其基因的酶。根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

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