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21. 发明授权

US06303757B1 Indirect polymerized and labelled antibody and method for manufacturing the same 失效
标题翻译：间接聚合和标记抗体及其制造方法
公开(公告)号：US06303757B1
公开(公告)日：2001-10-16
申请号：US09305289
申请日：1999-05-05
申请人： Nobuyuki Shigetou , Jinsei Miyazaki
发明人： Nobuyuki Shigetou , Jinsei Miyazaki
IPC分类号： C07K1702
CPC分类号： G01N33/58 , G01N33/532 , G01N33/583 , Y10S436/80
摘要： A high-sensitive indirect polymerized and labelled antibody is disclosed. The antibody facilitates detection of a low concentration of antigen as an analyte in a sample solution. The indirect polymerized and labelled antibody of the present invention is prepared by polymerizing an antibody using a multi-functional reagent, binding the polymerized antibody with a protein via a disulfide bond of the antibody to form a polymerized antibody conjugate, and labelling the conjugate with a cyanine dye represented by the following formula: where R1 and R2 represent hydrogen or an alkyl group, X represents a halogen, M represents hydrogen or an alkali metal, and n represents an integer of 1 to 4.
摘要翻译：公开了一种高灵敏度的间接聚合和标记的抗体。抗体有助于检测低浓度的抗原作为样品溶液中的分析物。本发明的间接聚合和标记的抗体是通过使用多功能试剂聚合抗体，通过抗体的二硫键将聚合的抗体与蛋白质结合形成聚合的抗体缀合物，并用偶联物标记缀合物来制备的由下式表示的花青染料：其中R1和R2表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。

22. 发明授权

US5965713A Dye labeled protein conjugate its preparing method and sensor using the same 失效
标题翻译：染料标记的蛋白质缀合物其制备方法和使用其的传感器
公开(公告)号：US5965713A
公开(公告)日：1999-10-12
申请号：US745337
申请日：1996-11-08
申请人： Nobuyuki Shigeto , Jinsei Miyazaki , Hiroshi Nakayama
发明人： Nobuyuki Shigeto , Jinsei Miyazaki , Hiroshi Nakayama
IPC分类号： G01N33/532 , C07K16/00 , C09B23/00 , G01N33/533 , A61K39/385
CPC分类号： G01N33/533 , C07K16/00 , Y10S530/802 , Y10S530/861 , Y10S530/866 , Y10T436/14
摘要： It is intended to present a sensor capable of labeling with more dyes, applying the labeled protein in immunochromatography making use of antigen-antibody reaction, and having an excellent sensitivity. In a buffer, a protein and a first covalent bonding compound that can react with this protein are reacted to prepare a protein conjugate, then a cyanine labeling dye is added in the buffer containing the protein conjugate, and the protein conjugate and cyanine labeling dye are reacted to prepare a dye labeled protein conjugate. Alternatively, in a buffer solution, a protein and a cyanine labeling dye are reacted to prepare a dye labeled protein, then a first covalent bonding compound that can react with the protein is added in the buffer containing the dye labeled protein, and the dye labeled protein and first covalent bonding protein are reacted to prepare a dye labeled protein conjugate.
摘要翻译：旨在提供能够用更多染料标记的传感器，在使用抗原 - 抗体反应的免疫色谱法中应用标记的蛋白质并具有优异的灵敏度。在缓冲液中，使蛋白质和可与该蛋白质反应的第一共价键合化合物进行反应以制备蛋白质缀合物，然后在含有蛋白质缀合物的缓冲液中加入花青标记染料，蛋白质缀合物和花青标记染料为反应以制备染料标记的蛋白质缀合物。或者，在缓冲溶液中，使蛋白质和花青标记染料反应以制备染料标记的蛋白质，然后将可与蛋白质反应的第一共价键合化合物加入到含有染料标记蛋白质的缓冲液中，染料标记蛋白质和第一共价键合蛋白反应以制备染料标记的蛋白质缀合物。

23. 发明授权

US5922618A Dye-labeled antibody conjugate and preparation method thereof 失效
标题翻译：染料标记的抗体缀合物及其制备方法
公开(公告)号：US5922618A
公开(公告)日：1999-07-13
申请号：US831204
申请日：1997-04-02
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
IPC分类号： G01N33/532 , G01N33/58 , G01N33/549 , C07K16/00
CPC分类号： G01N33/583 , G01N33/532 , Y10S436/80 , Y10S524/90
摘要： The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I . The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens. ##STR1## wherein X represents a halogen, M represents a hydrogen or alkali metal, and n represents an integer of 1 to 4
摘要翻译：本发明涉及包含抗体缀合物和花青染料的染料标记的抗体缀合物。通过使用多官能试剂如二硫代双（丙磺酸磺基琥珀酰亚胺酯）在磷酸盐缓冲液中聚合抗体如小鼠IgG，并搅拌该抗体和由式I表示的花青染料，来制备染料标记的抗体缀合物。染料标记的抗体缀合物比常规染料标记的抗体对抗原更敏感，因为它具有许多抗原反应性位点。其中X表示卤素，M表示氢或碱金属，n表示1〜4的整数

24. 发明授权

US06300480B1 Dye-labeled protein conjugate and method for preparing the same 失效
标题翻译：染料标记的蛋白质缀合物及其制备方法
公开(公告)号：US06300480B1
公开(公告)日：2001-10-09
申请号：US09310851
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Hiroshi Nakayama
IPC分类号： C07K1702
CPC分类号： G01N33/532 , G01N33/531 , Y10S436/80
摘要： The present invention provides a dye-labeled protein conjugate in which a protein conjugate is labeled with a large number of dye molecules. In the dye-labeled protein conjugate, a protein conjugate that includes a protein and an antibody bound thereto via a disulfide bond is labeled with a cyanine dye represented by the formula (1) given below. where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a rage of 1 to 4.
摘要翻译：本发明提供染料标记的蛋白质缀合物，其中蛋白质缀合物用大量染料分子标记。在染料标记的蛋白质缀合物中，包含蛋白质和通过二硫键结合的抗体的蛋白质缀合物用下述式（1）表示的花青染料标记。其中R1和R2表示氢或烷基 X表示卤素，M表示氢或碱金属，n表示1〜4的整数。

25. 发明授权

US5243074A Immunoreagents 失效
标题翻译：免责声明
公开(公告)号：US5243074A
公开(公告)日：1993-09-07
申请号：US616655
申请日：1990-11-21
申请人： Tadayasu Mitsumata , Jinsei Miyazaki
发明人： Tadayasu Mitsumata , Jinsei Miyazaki
IPC分类号： G01N33/533 , G01N33/58
CPC分类号： G01N33/533 , G01N33/582 , Y10S436/815 , Y10S436/816 , Y10S436/901
摘要： An immunofluorescent assay method, characterized in using an immunodetective reagent, which is an antigen-like substance comprising a fluorescent substance and an antigen chemically bonded thereto with or without intervention of an additional chemical bond, said fluorescent substance being changed in the wavelength or intensity of the fluorescence when an antibody comes close thereto, and said antigen being specific to said antibody.

26. 发明授权

US5233025A Amphetamine protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5233025A
公开(公告)日：1993-08-03
申请号：US651809
申请日：1991-02-07
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a higher affinity to methamphetamine that to the hapten N-(4-aminobutyl)amphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepeared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.
摘要翻译：已经合成了N-（4-氨基丁基）苯丙胺 - 蛋白质复合物作为免疫原，以制备对半抗原N-（4-氨基丁基）苯丙胺具有较高的对甲基苯丙胺的亲和力的抗体。通过使用该复合物制备的抗体与通过使用N-（4-氨基丁基）甲基苯丙胺 - 蛋白复合物作为免疫原而展示的抗体的甲基苯丙胺的亲和力为1,000倍。

27. 发明授权

US5229302A Fluorescence immunoassay method utilizing pseudo-antigens combined with fluorescent quenchers 失效
标题翻译：荧光免疫测定法利用假抗原与荧光猝灭剂结合
公开(公告)号：US5229302A
公开(公告)日：1993-07-20
申请号：US830442
申请日：1992-02-03
申请人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Noboru Motoyama , Tadayasu Mitsumata
IPC分类号： G01N33/532 , G01N33/542 , G01N33/94
CPC分类号： G01N33/542 , G01N33/532 , G01N33/946 , Y10S436/816
摘要： A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.
摘要翻译：描述了荧光免疫测定方法，其中通过测量从抗体发射的荧光的变化，在一分钟内测量抗原的存在或不存在。该方法包括通过抗原和荧光猝灭剂的化学组合获得的假抗原与通过猝灭剂组合的作用淬灭从抗体发射的荧光的抗体之间的反应。当将分析物加入到组合的溶液中时，如果分析物含有抗原，假抗原被抗原替代，使得荧光强度增加。测量该强度并与初始测量的强度进行比较以检测抗原的存在。该方法没有利用抗体对抗体对抗体的荧光的作用，适用于所有抗原。

28. 发明授权

US5026827A Amphetamine-protein complex as immunogen for obtaining antibodies specific to methamphetamine 失效
标题翻译：安非他明 - 蛋白复合物作为获得甲基苯丙胺特异性抗体的免疫原
公开(公告)号：US5026827A
公开(公告)日：1991-06-25
申请号：US401247
申请日：1989-08-31
申请人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
发明人： Jinsei Miyazaki , Makoto Taketani , Tadayasu Mitsumata
IPC分类号： A61K47/48 , C07K16/44 , G01N33/531
CPC分类号： G01N33/531 , A61K47/4833 , C07K16/44
摘要： A N-(4-aminobutyl)amphetamine-protein complex has been synthesized to be used as an immunogen to prepare an antibody having a high affinity to methamphetamine. The antibody prepared by the use of the complex has 1,000 times the affinity to methamphetamine as that of an antibody prepared by the use of N-(4-aminobutyl)methamphetamine-protein complex as an immunogen.

29. 发明授权

US4845021A Method for recording optical information in optical high density recording mediums 失效
标题翻译：在光学高密度记录介质中记录光学信息的方法
公开(公告)号：US4845021A
公开(公告)日：1989-07-04
申请号：US145686
申请日：1988-01-14
申请人： Jinsei Miyazaki , Eiji Ando , Kimiaki Yoshino , Kazuhisa Morimoto
发明人： Jinsei Miyazaki , Eiji Ando , Kimiaki Yoshino , Kazuhisa Morimoto
IPC分类号： G11B7/00 , G11B7/0045 , G11B7/244 , G11B7/26
CPC分类号： G11B7/26 , G11B7/00455 , G11B7/244 , G11B7/2472 , G11B2007/0013 , G11B7/2475 , Y10S430/146 , Y10S430/163
摘要： An optical recording medium which comprises a substrate and a recording layer of at least two J-aggregates of dyes formed on the substrate is described. The dyes may be photochromic dyes or other types of dyes capable of forming the aggregates which have narrower absorption spectral ranges than original dyes. A plurality of information bits can be written in the recording layer by irradiation of one laser beam spot. The recording layer is formed by an LB film method or a method capable of fluidizing a dye solution in a direction horizontal to the substrate surface. A recording method using the medium is also described.

30. 发明授权

US4737427A Optical high density recording mediums, method for making same and method for recording optical information in the medium 失效
标题翻译：光学高密度记录介质，其制造方法以及在介质中记录光学信息的方法
公开(公告)号：US4737427A
公开(公告)日：1988-04-12
申请号：US836625
申请日：1986-03-05
申请人： Jinsei Miyazaki , Eiji Ando , Kimiaki Yoshino , Kazuhisa Morimoto
发明人： Jinsei Miyazaki , Eiji Ando , Kimiaki Yoshino , Kazuhisa Morimoto
IPC分类号： G11B7/00 , G11B7/0045 , G11B7/244 , G11B7/26 , G03C11/00 , F23N5/00 , G01D9/00
CPC分类号： G11B7/26 , G11B7/00455 , G11B7/244 , G11B7/2472 , G11B2007/0013 , G11B7/2475 , Y10S430/146 , Y10S430/163
摘要： A optical recording medium which comprises a substrate and a recording layer of at least two J-aggregates of dyes formed on the substrate is described. The dyes may be photochromic dyes or other types of dyes capable of forming the aggregates which have narrower absorption spectral ranges than original dyes. A plurality of information bits can be written in the recording layer by irradiation of one laser beam spot. The recording layer is formed by an LB film method or a method capable of fluidizing a dye solution in a direction horizontal to the substrate surface. A recording method using the medium is also described.
摘要翻译：描述了一种光记录介质，其包括基板和形成在基板上的至少两个J聚集体的记录层。染料可以是光致变色染料或能够形成具有比原始染料更窄的吸收光谱范围的聚集体的其它类型的染料。可以通过一个激光束点的照射将多个信息位写入记录层。记录层通过LB膜法或能够使染料溶液在与基板表面水平的方向上流化的方法形成。还描述了使用该介质的记录方法。

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