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21. 发明授权

US08394609B2 Primers and methods for the detection and discrimination of nucleic acids 有权
公开(公告)号：US08394609B2
公开(公告)日：2013-03-12
申请号：US12629045
申请日：2009-12-01
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12P19/34
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

22. 发明申请

US20120142007A1 STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 失效
标题翻译：用于核酸扩增和测序的稳定组合物
公开(公告)号：US20120142007A1
公开(公告)日：2012-06-07
申请号：US13345632
申请日：2012-01-06
申请人： Ayoub Rashtchian , Joseph Solus
发明人： Ayoub Rashtchian , Joseph Solus
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C12N9/12
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
摘要翻译：本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性延长的时间。这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶各种医疗，法医和农业应用。特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

23. 发明申请

US20090233283A1 STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 审中-公开
标题翻译：用于核酸扩增和测序的稳定组合物
公开(公告)号：US20090233283A1
公开(公告)日：2009-09-17
申请号：US12129597
申请日：2008-05-29
申请人： Ayoub Rashtchian , Joseph Solus
发明人： Ayoub Rashtchian , Joseph Solus
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
摘要翻译：本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性较长时间。这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶各种医疗，法医和农业应用。特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

24. 发明授权

US07537886B1 Primers and methods for the detection and discrimination of nucleic acids 有权
标题翻译：用于检测和鉴别核酸的引物和方法
公开(公告)号：US07537886B1
公开(公告)日：2009-05-26
申请号：US09599594
申请日：2000-06-22
申请人： Irina Nazarenko , Ayoub Rashtchian
发明人： Irina Nazarenko , Ayoub Rashtchian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6816 , C12Q1/6818 , C12Q1/6832 , C12Q1/6851 , C12Q1/6858 , C12Q2565/107 , C12Q2563/107 , C12Q2561/12 , C12Q2561/119 , C12Q2525/301 , C12Q2525/101
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

25. 发明申请

US20050123924A1 Compositions for in vitro amplification of nucleic acids 审中-公开
标题翻译：用于体外扩增核酸的组合物
公开(公告)号：US20050123924A1
公开(公告)日：2005-06-09
申请号：US10633629
申请日：2003-08-05
申请人： Ayoub Rashtchian , David Schuster
发明人： Ayoub Rashtchian , David Schuster
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2527/125
摘要： Method and compositions for improving DNA polymerase and reverse transcriptase reactions are provided. Addition of anti-foam reagents to the reactions improves fluid handling, especially of small volumes and allows enhanced accuracy of optical detection, without substantially inhibiting enzymatic activity.
摘要翻译：提供了改进DNA聚合酶和逆转录酶反应的方法和组合物。将反泡沫试剂加入到反应中改善了流体处理，特别是小体积的流动处理，并且允许光学检测的增加的准确性，而基本上不抑制酶活性。

26. 发明授权

US5447848A Detection of campylobacter with nucleic acid probes 失效
标题翻译：用核酸探针检测弯曲杆菌
公开(公告)号：US5447848A
公开(公告)日：1995-09-05
申请号：US216679
申请日：1988-07-07
申请人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
发明人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N33/50 , C07H21/00 , C12Q1/02
CPC分类号： C12Q1/6813 , C12Q1/689
摘要： Nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium are described along with methods utilizing such probes for the detection of Campylobacter in clinical, food and other samples.
摘要翻译：与能够与空肠弯曲杆菌，大肠杆菌和C.laridis的rRNA特异性杂交的核酸探针以及不属于恶臭假单胞菌（Pseudomonas aeuroginosa），大肠杆菌或鼠伤寒沙门氏菌的rRNA或rRNA基因的核酸探针与使用这种探针用于检测弯曲杆菌在临床，食品等样品中。

27. 发明授权

US5137814A Use of exo-sample nucleotides in gene cloning 失效
标题翻译：在基因克隆中使用外切核苷酸
公开(公告)号：US5137814A
公开(公告)日：1992-08-11
申请号：US715623
申请日：1991-06-14
申请人： Ayoub Rashtchian , Mark S. Berninger
发明人： Ayoub Rashtchian , Mark S. Berninger
IPC分类号： C12M1/00 , C12N15/09 , C12N15/10 , C12N15/64 , C12N15/66
CPC分类号： C12N15/102 , C12N15/64 , C12N15/66
摘要： The present invention provides improved methods for manipulating recombinant DNA in gene cloning and expression. More specifically, the invention provides methods capable of altering a nucleic acid sequence present at the termini of a target sequence.
摘要翻译：本发明提供了在基因克隆和表达中操纵重组DNA的改进方法。更具体地，本发明提供能够改变存在于靶序列末端的核酸序列的方法。

28. 发明授权

US08124340B2 Methods for enrichment of selected RNA molecules 有权
标题翻译：富集选定RNA分子的方法
公开(公告)号：US08124340B2
公开(公告)日：2012-02-28
申请号：US12692592
申请日：2010-01-23
申请人： Ayoub Rashtchian
发明人： Ayoub Rashtchian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2521/107 , C12Q2521/327 , C12Q2525/101 , C12Q2525/207 , C12Q2521/319
摘要： Improved methods of studying RNA molecules are provided. In particular, methods of treating mixtures of RNA molecules so as to enrich the mixture for a desired type of RNA molecule are provided. For example, the methods permit depletion of mRNA from complex mixtures to facilitate study of microRNAs in the mixture.
摘要翻译：提供了改进的研究RNA分子的方法。特别地，提供了处理RNA分子的混合物以便富集所需类型的RNA分子的混合物的方法。例如，该方法允许从复杂混合物中消耗mRNA，以促进混合物中微小RNA的研究。

29. 发明授权

US07883871B2 Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR) 有权
标题翻译：逆转录聚合酶链反应（RT-PCR）的组合物和方法
公开(公告)号：US07883871B2
公开(公告)日：2011-02-08
申请号：US10861469
申请日：2004-06-07
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

30. 发明申请

US20100297709A1 Methods for enrichment of selected RNA molecules 有权
标题翻译：富集选定RNA分子的方法
公开(公告)号：US20100297709A1
公开(公告)日：2010-11-25
申请号：US12692592
申请日：2010-01-23
申请人： Ayoub Rashtchian
发明人： Ayoub Rashtchian
IPC分类号： C12P19/34
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2521/107 , C12Q2521/327 , C12Q2525/101 , C12Q2525/207 , C12Q2521/319
摘要： Improved methods of studying RNA molecules are provided. In particular, methods of treating mixtures of RNA molecules so as to enrich the mixture for a desired type of RNA molecule are provided. For example, the methods permit depletion of mRNA from complex mixtures to facilitate study of microRNAs in the mixture.
摘要翻译：提供了改进的研究RNA分子的方法。特别地，提供了处理RNA分子的混合物以便富集所需类型的RNA分子的混合物的方法。例如，该方法允许从复杂混合物中消耗mRNA，以促进混合物中微小RNA的研究。

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