专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明申请

US20210104299A1 Analysis Method for Glycoproteins 有权
公开(公告)号：US20210104299A1
公开(公告)日：2021-04-08
申请号：US17042108
申请日：2019-03-19
申请人： DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
发明人： Takashi Baba
IPC分类号： G16B40/10 , G01N33/68 , G16C20/20 , H01J49/00
摘要： A mass isolation device selects a precursor ion of a sample that has been digested using a protease. A first fragmentation device fragments the precursor ion using collision-induced dissociation (CID), and the resulting product ions are analyzed using a mass analyzer producing a CID spectrum. A list of theoretical candidate glycopeptide sequences is determined from CID spectrum. The mass isolation device again selects the precursor ion of the sample. A second fragmentation device fragments the precursor ion using electron-based dissociation (ExD), and the resulting product ions are analyzed using the mass analyzer producing a CID spectrum. For each sequence of the list, the sequence is computationally fragmented, producing theoretical fragments, mass-to-charge ratio (m/z) values are calculated for the theoretical fragments, and the sequence is scored using c and z fragment matching rules. The highest scoring sequence is identified as a peptide sequence of a glycopeptide of the sample.

22. 发明申请

US20210082678A1 Top Down Analysis of Antibodies in Mass Spectrometry 有权
公开(公告)号：US20210082678A1
公开(公告)日：2021-03-18
申请号：US17041324
申请日：2019-04-09
申请人： DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
发明人： Takashi Baba , Pavel Ryumin , William M. Loyd
IPC分类号： H01J49/40 , G01N33/68 , G16B40/10
摘要： A separation device separates an unknown intact mAb or reduced mAb subunits of a known mAb class from a sample. M An ion source device ionizes the mAb. A mass spectrometer fragments the ionized mAb using an ECD device and mass analyzes resulting product ions using a mass analyzer, producing one or more product ion spectra. Theoretical product ion peaks are calculated for one or more constant portions of the mAb class. The theoretical product ion peaks are removed from the one or more product ion spectra, producing one or more difference product ion spectra. De novo sequencing is applied to the one or more difference product ion spectra, producing one or more candidate sequences for one or more variable portions of the mAb. A genome database is searched for matches to the one or more candidate sequences, producing one or more matched sequences for the one or more variable portions.

23. 发明授权

US10256087B2 Band pass extraction from an ion trapping device and TOF mass spectrometer sensitivity enhancement 有权
公开(公告)号：US10256087B2
公开(公告)日：2019-04-09
申请号：US15329376
申请日：2015-07-24
申请人： DH Technologies Development PTE Ltd.
发明人： Takashi Baba
IPC分类号： H01J49/00 , H01J49/40 , H01J49/42 , H01J49/06
摘要： A multipole rod set of an ion guide is adapted to receive a radial RF trapping voltage and a radial dipole direct current DC voltage. A lens electrode of the ion guide is positioned at one end of the multipole rod set to extract ions from the multipole rod set and adapted to receive an axial trapping AC voltage and a DC voltage. A radial dipole DC voltage is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to a lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set. Alternatively, a radial RF trapping voltage amplitude is applied to the multipole rod set and an axial trapping AC voltage is simultaneously applied to the lens electrode in order to extract a bandpass mass range of ions trapped in the multipole rod set.

24. 发明授权

US10256083B2 Multiplexed precursor isolation for mass spectrometry 有权
公开(公告)号：US10256083B2
公开(公告)日：2019-04-09
申请号：US15089527
申请日：2016-04-02
申请人： DH Technologies Development Pte. Ltd.
发明人： Takashi Baba
IPC分类号： H01J49/00 , H01J49/06 , H01J49/04 , H01J49/42
摘要： Systems and methods for identifying precursor ions of product ions from combined product ion spectra are provided. N precursor ions are selected. N groups of the N precursor ions are created. The tandem mass spectrometer is instructed to perform multiplexed precursor ion selection on the continuous beam of ions, fragment each of the N−1 precursor ions, and measure the intensities of the product ions, producing N product ion spectra. A heat map is plotted, producing N heat maps. The N product ion spectra are combined into a combined product ion spectrum. A corresponding precursor ion of a peak is identified by finding a heat map of the N heat maps that does not have data for the mass of the peak and determining that a precursor ion of the N precursor ions that is not included in a group that produced the heat map is the corresponding precursor ion.

25. 发明申请

US20190051506A1 Ion Injection Method Into Side-On FT-ICR Mass Spectrometers 审中-公开
公开(公告)号：US20190051506A1
公开(公告)日：2019-02-14
申请号：US16069506
申请日：2017-01-10
申请人： DH Technologies Development Pte. Ltd.
发明人： Takashi Baba
IPC分类号： H01J49/38 , H01J49/42 , H01J49/10
CPC分类号： H01J49/38 , H01J49/102 , H01J49/422 , H01J49/4235 , H01J49/426
摘要： Improvements to a side-on Penning trap include methods to stabilize ions in the trap. The ions are stabilized by injecting ions in the focusing region of the non-uniform DC fields produced by the pad electrodes of the trap. Ions are injected along an injection axis shifted from the central axis of a gap between a positively biased electrode pad and negatively biased electrode pad of the trap. Improvements also include methods to compensate for the Lorentz force that is produced when ions are injected into a side-on Penning trap. Electrodes of an ion injection device are DC biased so that the electrodes produce an electric field along the axis of the device that compensates for the Lorentz force. Finally, methods are provided to increase the m/z range of ions injected into a side-on Penning trap by pre-trapping ions just before injection of the ions into the trap.

26. 发明授权

US10014166B2 Inline ion reaction device cell and method of operation 有权
公开(公告)号：US10014166B2
公开(公告)日：2018-07-03
申请号：US14894359
申请日：2014-05-29
申请人： DH Technologies Development Pte. Ltd.
发明人： Takashi Baba
IPC分类号： H01J49/00 , H01J49/06
CPC分类号： H01J49/0054 , H01J49/0045 , H01J49/0072 , H01J49/063
摘要： A method and apparatus for conducting ion to charged species reactions, more particularly reactions wherein the charged species is an electron, such as ECD. The apparatus comprises first and second pathways which are orthogonal to one another. The first pathway through which ions are introduced comprises multiple multipoles with a gap situated there between. The second pathway introduces the charged species through the gap orthogonally to the first pathway. In this way, a cross-type reaction device allows ion-charged species interactions to occur.

27. 发明申请

US20180095092A1 Top Down Protein Identification Method 审中-公开
公开(公告)号：US20180095092A1
公开(公告)日：2018-04-05
申请号：US15573594
申请日：2016-05-10
申请人： DH Technologies Development PTE Ltd.
发明人： Takashi Baba
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6848 , G01N2560/00 , H01J49/0054
摘要： Systems and methods described herein can provide for “top down” mass spectrometric analysis of proteins or peptides in a sample using ExD, in some aspects via direct infusion of the sample to the ion source without on-line LC separation, while deconvoluting the ambiguity in the ExD spectra generated by impure samples. For example, methods and systems in accordance with various aspects of the present teachings can utilize patterns in charge-reduced species following ExD to correlate the ExD fragments with their precursor ions in order to more confidently identify the precursor ion from which the detected product ions originated.

28. 发明申请

US20160322208A1 SIMULTANEOUS POSITIVE AND NEGATIVE ION ACCUMULATION IN AN ION TRAP FOR MASS SPECTROSCOPY 有权
标题翻译：用于大量光谱的离子阱同时积极和负离子累积
公开(公告)号：US20160322208A1
公开(公告)日：2016-11-03
申请号：US15105130
申请日：2014-11-18
申请人： DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
发明人： Takashi Baba
IPC分类号： H01J49/00 , H01J49/42
CPC分类号： H01J49/0095 , H01J49/0072 , H01J49/062 , H01J49/4225 , H01J49/4295
摘要： An ion reaction device is provided having an ion injection inlet for receiving a plurality of ions and an ion ejection outlet through which ions can exit the device. The reaction device includes a plurality of non-linear rods disposed relative to one another so as to provide an axial region configured to receive a plurality of anions and cations via said ion injection inlet, and a plurality of trapping regions in communication with the axial region in which said anions and cations can be confined. A DC voltage source is adapted to apply a DC voltage across at least two of said rods so as to generate an electric field within at least a portion of said axial region for spatially separating the received anions and cations and guiding the anions into one of said trapping regions and the cations into another one of said trapping regions.
摘要翻译：提供了一种离子反应装置，其具有用于接收多个离子的离子注入口和离子可以离开装置的离子喷射出口。反应装置包括相对于彼此设置的多个非线性杆，以提供构造成经由所述离子注入口接收多个阴离子和阳离子的轴向区域，以及与轴向区域连通的多个捕获区域其中阴离子和阳离子可以被限制。直流电压源适于跨所述杆中的至少两个施加直流电压，以便在所述轴向区域的至少一部分内产生电场，用于空间分离所接收的阴离子和阳离子，并将阴离子引导到所述捕获区域和阳离子到另一个所述捕获区域。

29. 发明申请

US20220375736A1 Method for Top Down Proteomics Using ExD and PTR 有权
公开(公告)号：US20220375736A1
公开(公告)日：2022-11-24
申请号：US17650836
申请日：2022-02-11
申请人： DH TECHNOLOGIES DEVELOPMENT PTE. LTD.
发明人： Takashi Baba , Pavel Ryumin , William M. Loyd
IPC分类号： H01J49/00 , H01J3/40 , H01J29/84 , H01J49/06
摘要： A dissociation device fragments a precursor ion, producing at least two different product ions with overlapping m/z values in the dissociation device. The dissociation device applies an AC voltage and a DC voltage creating a pseudopotential that traps ions below a threshold m/z including the at least two product ions. The dissociation device receives a charge reducing reagent that causes the trapped at least two product ions to be charge reduced until their m/z values increase above the threshold m/z set by the AC voltage. The increase in the m/z values of the at least two product ions decreases their overlap. The at least two product ions with increased m/z values are transmitted to another device for subsequent mass analysis by applying the DC voltage to the dissociation device relative to a DC voltage applied to the other device.

30. 发明申请

US20220155318A1 BACKGROUND REDUCTION IN TOP-DOWN ANTIBODY ANALYSIS 有权
公开(公告)号：US20220155318A1
公开(公告)日：2022-05-19
申请号：US17440130
申请日：2020-05-13
申请人： DH Technologies Development PTE. LTD.
发明人： Takashi Baba , Pavel Ryumin
IPC分类号： G01N33/68 , H01J49/42
摘要： Method and devices for performing top down analysis of an antibody are described which involve utilizing an ion source to generate a plurality of ions from a sample containing at least one intact antibody. Further, transmitting said plurality of ions through a quadrupole rod set while applying an RF signal thereto and in the absence of a resolving DC voltage so as to preferentially transmit precursor ions having an m/z value greater than a low mass cutoff of about 1500 m/z from the quadrupole rod set to an ECD cell. The method and device can also performing an ECD reaction in the ECD cell on said precursor ions and also detect reaction products from the ECD reaction.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式