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21. 发明申请

US20110217723A1 EPI-ILLUMINATION APPARATUS AND METHODS FOR SORTING PARTICLES 有权
标题翻译： EPI-照明装置和用于分选颗粒的方法
公开(公告)号：US20110217723A1
公开(公告)日：2011-09-08
申请号：US13106705
申请日：2011-05-12
申请人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
发明人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
IPC分类号： C12Q1/02 , C12M1/34
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A method and apparatus for sorting stained particles in a fluid stream, each of the method and apparatus including an epi-illumination optics system with a focusing lens. The optics system being operable to direct a beam of electromagnetic radiation through the focusing lens in a forward direction along a beam axis intersecting particles in the fluid stream at said first location so that said particles pass through the beam, resulting in the fluorescence emissions from the particles being directed along said beam axis in a rearward direction.
摘要翻译：一种用于在流体流中分选染色颗粒的方法和装置，所述方法和装置中的每一个包括具有聚焦透镜的外延照明光学系统。所述光学系统可操作以沿着与所述第一位置处的所述流体流中的颗粒相交的束轴在向前方向上引导电磁辐射束通过所述聚焦透镜，使得所述颗粒通过所述光束，导致来自所述光束的荧光发射颗粒沿着所述光束轴线沿向后方向被引导。

22. 发明申请

US20090176271A1 Systems for Efficient Staining and Sorting of Populations of Cells 有权
标题翻译：高效染色和分选细胞群的系统
公开(公告)号：US20090176271A1
公开(公告)日：2009-07-09
申请号：US12404931
申请日：2009-03-16
申请人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
发明人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
IPC分类号： G01N1/30
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.
摘要翻译：一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。集成平台可以包括电磁辐射的共同来源。本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

23. 发明申请

US20050112541A1 Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm 有权
标题翻译：用于分选颗粒和提供性别分类的动物精子的装置，方法和方法
公开(公告)号：US20050112541A1
公开(公告)日：2005-05-26
申请号：US10812351
申请日：2004-03-29
申请人： Gary Durack , Jeffrey Wallace , Gary Vandre , Lon Westfall , Jeremy Hatcher , Niraj Nayak
发明人： Gary Durack , Jeffrey Wallace , Gary Vandre , Lon Westfall , Jeremy Hatcher , Niraj Nayak
IPC分类号： A01N1/02 , C12M1/34 , C12N5/071 , C12Q1/04 , G01N1/30 , G01N15/00 , G01N15/14 , G01N33/48 , G01N33/487 , G01N33/50
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.
摘要翻译：一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。集成平台可以包括电磁辐射的共同来源。本发明的方法包括使用共享该集成平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

24. 发明申请

US20120091366A1 SYSTEM AND METHOD FOR THE MEASUREMENT OF MULTIPLE FLUORESCENCE EMISSIONS IN A FLOW CYTOMETRY SYSTEM 有权
标题翻译：用于测量流动细胞计数系统中多种荧光发射的系统和方法
公开(公告)号：US20120091366A1
公开(公告)日：2012-04-19
申请号：US13329555
申请日：2011-12-19
申请人： Gary Durack , Jeremy Hatcher
发明人： Gary Durack , Jeremy Hatcher
IPC分类号： G01N21/64
CPC分类号： G01N15/1459
摘要： A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed.
摘要翻译：公开了一种在流式细胞仪系统中测量多种荧光发射的系统和方法，其中每个激发光源以不同的频率进行调制。使用单个检测器来收集由所有光源激发的荧光发射，并且使用傅里叶变换技术将发射分离。还公开了用于校正光束间重合的系统和方法。

25. 发明申请

US20120001090A1 MINUTE PARTICLE ANALYZING DEVICE AND METHOD 有权
标题翻译：分钟颗粒分析装置和方法
公开(公告)号：US20120001090A1
公开(公告)日：2012-01-05
申请号：US12829070
申请日：2010-07-01
申请人： Koji Takasaki , Katsuhiro Seo , Mitsuru Toishi , Shinji Yamada , Atsushi Fukumoto , Gary Durack
发明人： Koji Takasaki , Katsuhiro Seo , Mitsuru Toishi , Shinji Yamada , Atsushi Fukumoto , Gary Durack
IPC分类号： G01J1/58
CPC分类号： G01N15/1434 , G01J3/024 , G01J3/1256 , G01N15/1459 , G01N21/53 , G01N21/645 , G02B6/14 , G02B6/32 , G02B6/4215
摘要： A minute particle analyzing device includes: a light source; a first condenser lens for condensing light from the light source to a first end of a multimode optical fiber; a second condenser lens for condensing the light emerging from a second end of the multimode optical fiber to a minute particle; and a detector for detecting light generated from the minute particle by the application of the light from the light source.
摘要翻译：微粒分析装置包括：光源; 用于将来自光源的光聚焦到多模光纤的第一端的第一聚光透镜; 第二聚光透镜，用于将从多模光纤的第二端出射的光聚焦到微小颗粒; 以及检测器，用于通过施加来自光源的光来检测由微粒产生的光。

26. 发明申请

US20110020855A1 Method and apparatus for performing cytometry 审中-公开
标题翻译：用于进行细胞计数的方法和装置
公开(公告)号：US20110020855A1
公开(公告)日：2011-01-27
申请号：US12660570
申请日：2010-03-01
申请人： Masataka Shinoda , Gary Durack
发明人： Masataka Shinoda , Gary Durack
IPC分类号： C12Q1/02 , C12M1/00
CPC分类号： G01N15/1484 , G01N2015/1006 , G01N2015/149
摘要： Embodiments of the present disclosure generally include a method and apparatus for performing cytometry. Specifically, embodiments of the invention comprise apparatus for providing a sample fluid to a cytometry system comprising a cytometry chip and a holder. The cytometry chip is for channeling a sample fluid from a sample fluid port to an output channel. The holder is configured to retain the cytometry chip within the holder. The holder comprises an interface between the cytometry chip and at least one of a source of the sample fluid, a source of a sheath fluid or a source of electric charge. Embodiments of a method comprise using the apparatus to provide a sample fluid to a cytometry system.
摘要翻译：本公开的实施方案通常包括用于进行细胞计数的方法和装置。具体地，本发明的实施方案包括用于向包括细胞计数芯片和保持器的细胞计数系统提供样品流体的装置。细胞计数芯片用于将样品流体从样品流体端口引导到输出通道。保持器被配置为将细胞计数芯片保持在保持器内。保持器包括细胞计数芯片与样品流体源，鞘液源或电荷源中的至少一个之间的界面。方法的实施方案包括使用该装置将样品流体提供给细胞计数系统。

27. 发明授权

US07758811B2 System for analyzing particles using multiple flow cytometry units 有权
标题翻译：使用多种流式细胞仪分析颗粒的系统
公开(公告)号：US07758811B2
公开(公告)日：2010-07-20
申请号：US10812351
申请日：2004-03-29
申请人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
发明人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
IPC分类号： G01N33/48 , C12M1/34 , G01N21/64
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A multichannel system for classifying particles according to one or more characteristics of the particles includes a plurality of flow cytometry units. Each flow cytometry unit is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing particles using a beam of electromagnetic radiation. Each flow cytometry unit has a sensor operable to generate a time-varying output signal indicative of at least one characteristic of the particles in the stream of fluid as the stream of fluid is interrogated by the beam of electromagnetic radiation. The flow cytometry units share an integrated platform and perhaps even a common processor for receiving and processing information from the units. The common processor is programmed to receive the time-varying output signals from the flow cytometry units substantially continuously and to process the output signals.
摘要翻译：用于根据颗粒的一个或多个特征分级颗粒的多通道系统包括多个流式细胞术单元。每个流式细胞计量单元可操作以通过使用电磁辐射束询问含有颗粒的流的流来分类粒子混合物中的颗粒。每个流式细胞计量单元具有可操作的传感器，用于当电磁辐射束询问流体流时，产生指示流体流中颗粒的至少一个特征的时变输出信号。流式细胞仪单元共享一个集成的平台，甚至可能是一个通用的处理器，用于从单元接收和处理信息。公共处理器被编程为基本上连续地从流式细胞仪单元接收时变输出信号并处理输出信号。

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