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21. 发明申请

US20070274861A1 MEMBRANES AND METHODS FOR COATING MEMBRANES 有权
标题翻译：膜和涂膜方法
公开(公告)号：US20070274861A1
公开(公告)日：2007-11-29
申请号：US11758804
申请日：2007-06-06
申请人： Juergen Klepp , Thomas Fischer , Rolf Lerch , Dieter Mangold , Juergen Schaeffler
发明人： Juergen Klepp , Thomas Fischer , Rolf Lerch , Dieter Mangold , Juergen Schaeffler
IPC分类号： G01N31/22
CPC分类号： B01D69/12 , B01D67/0088 , G01N33/521 , Y10T436/2575
摘要： A method for applying reactive films containing solids to microporous membranes is provided wherein the membrane is firstly moistened and the reactive film containing solids is applied to the membrane while it is still moist. Membranes produced in this manner can contain reactive films having a high proportion of film openers and can be used advantageously in diagnostic elements for detecting constituents and, in particular, large hydrophobic analytes in body fluid.
摘要翻译：提供了一种将含有固体的反应性膜施加到微孔膜的方法，其中首先润湿膜，并且将含有固体的反应性膜施加到膜上，同时其仍然是潮湿的。以这种方式制备的膜可以含有具有高比例的开膜剂的反应性膜，并且可以有利地用于检测成分的诊断元件，特别是用于体液中的大的疏水性分析物。

22. 发明授权

US5306622A Heterogeneous immunoassay process 失效
标题翻译：非均质免疫测定法
公开(公告)号：US5306622A
公开(公告)日：1994-04-26
申请号：US788173
申请日：1991-11-05
申请人： Dieter Mangold
发明人： Dieter Mangold
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54366 , G01N33/5302 , G01N33/54306 , Y10S435/962 , Y10S435/967 , Y10S435/97 , Y10S435/973 , Y10S436/805 , Y10S436/81
摘要： The invention teaches a method for determining an analyte in a liquid sample via a heterogeneous assay. The sample is contacted to a carrier material which contains, in addition to one of a labelled analyte analogous substance or a labelled analyte specific substance, a detectable component which does not influence immunological reactions occurring during the course of the assay. A solid phase bound component, which is either an insufficient amount of analyte specific substance or an excess of analyte relative to analyte in the sample is contacted to the sample so as to accomplish formation of solid phase bound complexes. After separation of liquid and solid phase, label is measured in one of these, and the detectable substance is measured in the liquid. Correlation of the values obtained leads to determination of the analyte. Also described is a reagent used in the described method.
摘要翻译：本发明教导了通过异质测定法确定液体样品中的分析物的方法。样品与载体材料接触，载体材料除了标记的分析物类似物质或标记的分析物特异物质之外，还含有不影响在测定过程中发生的免疫反应的可检测组分。与样品中的分析物相比，分析物特异性物质的量不足或分析物过量的固相结合成分与样品接触，以实现固相结合复合物的形成。在分离液相和固相后，在其中之一中测量标记，并在液体中测量可检测物质。所获得的值的相关性导致分析物的测定。还描述了所述方法中使用的试剂。

23. 发明授权

US5296356A Enzyme-immunoassay method for the determination of an analyte 失效
标题翻译：用于测定分析物的酶 - 免疫测定方法
公开(公告)号：US5296356A
公开(公告)日：1994-03-22
申请号：US626257
申请日：1990-12-12
申请人： Dieter Mangold , Ulrich Traeger , Hans Lange
发明人： Dieter Mangold , Ulrich Traeger , Hans Lange
IPC分类号： G01N33/543 , G01N37/00
CPC分类号： G01N33/54386 , G01N33/54366 , Y10S435/97 , Y10S436/81
摘要： The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the concentration of the analyte. The measurement is carried out in a non-porous molding having the liquid phase contained therein in contact with the solid phase. The method is particularly suitable for carrying out in a cuvette which can be filled via the porous matrix or on a test strip having a space in contact with the solid phase.
摘要翻译：本发明涉及通过酶 - 免疫测定来检测样品液体中的分析物的方法，其中酶标记的化合物在固相和液相之间分配，并且在固相外的液相中的酶标记物的量被确定为分析物浓度的量度。该测量在其中含有液相与固相接触的无孔模制品中进行。该方法特别适于在可以通过多孔基质填充的比色皿中或在具有与固相接触的空间的测试条上进行。

24. 发明授权

US5188939A Displacement immunoassay utilizing an oligavalent labelled antibody 失效
标题翻译：使用免疫反应标签的免疫球蛋白
公开(公告)号：US5188939A
公开(公告)日：1993-02-23
申请号：US549893
申请日：1990-07-09
申请人： Dieter Mangold , Reiner Schlipfenbacher
发明人： Dieter Mangold , Reiner Schlipfenbacher
IPC分类号： G01N33/53 , G01N33/532 , G01N33/535 , G01N33/543 , G01N33/68
CPC分类号： G01N33/54386 , G01N33/535 , G01N33/6857 , Y10S436/819
摘要： A method for carrying out competitive displacement immunoassays which entails the use of an oligovalent labelled antibody having at least 4 binding sites for the analyte to be determined per label.
摘要翻译：用于进行竞争性置换免疫测定的方法，其需要使用具有至少4个结合位点的寡核苷酸标记抗体来分析每个标签。

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