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11. 发明授权

US4331590A .beta.-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates 失效
标题翻译： β-半乳糖基 - 伞形酮酮标记的蛋白质和多肽缀合物
公开(公告)号：US4331590A
公开(公告)日：1982-05-25
申请号：US147339
申请日：1980-05-06
申请人： Robert C. Bocuslaski , John F. Burd , Robert J. Carrico
发明人： Robert C. Bocuslaski , John F. Burd , Robert J. Carrico
IPC分类号： C07H17/075 , G01N33/533 , G01N33/535 , G01N33/58 , A61K39/385 , A61K39/44 , C07G7/00
CPC分类号： C07H17/075 , G01N33/533 , G01N33/535 , G01N33/582 , Y10S436/80 , Y10S436/805 , Y10S436/823 , Y10S530/806
摘要： An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G-D-Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.
摘要翻译：用于测定液体培养基中配体的改进的特异性结合测定方法和试剂，其使用具有下式的GDR的残基作为可酶切的底物标记，其中G是糖苷，D是染料指示剂部分，R是连接基团，其中标记残基与常规结合测定系统（例如配体，其类似物或其特异性结合配偶体）的结合成分共价结合。标记物的监测特征是在酶切割糖基与染料指示剂部分之间的糖苷键时可释放的产物（通常为氟或色原体）的释放。测定方法可以遵循均匀或异质的方式。优选的糖酮是β-半乳糖基，优选的染料指示剂部分是伞形酮残基。改进的测定法特别适合于均相竞争性结合测定形式之后测定半抗原，例如药物和抗原蛋白质和多肽，包括抗体。

12. 发明授权

US4279992A Specific binding assay employing an enzyme-cleavable substrate as label 失效
标题翻译：使用酶切割底物作为标记的特异性结合测定
公开(公告)号：US4279992A
公开(公告)日：1981-07-21
申请号：US87819
申请日：1979-10-23
申请人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
发明人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
IPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , G01N33/533 , G01N33/535 , G01N33/54 , C12N9/96 , C12Q1/34 , C12Q1/44
CPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , G01N33/533 , G01N33/535 , Y10S435/81 , Y10S435/961 , Y10S436/80 , Y10S436/815 , Y10S436/816
摘要： An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G--D--Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.
摘要翻译：用于测定液体培养基中配体的改进的特异性结合测定方法和试剂，其使用具有下式的GDR的残基作为可酶切的底物标记，其中G是糖苷，D是染料指示剂部分，R是连接基团，其中标记残基与常规结合测定系统（例如配体，其类似物或其特异性结合配偶体）的结合成分共价结合。标记物的监测特征是在酶切割糖基与染料指示剂部分之间的糖苷键时可释放的产物（通常为氟或色原体）的释放。测定方法可以遵循均匀或异质的方式。优选的糖酮是β-半乳糖基，优选的染料指示剂部分是伞形酮残基。改进的测定法特别适合于均相竞争性结合测定形式之后测定半抗原，例如药物和抗原蛋白质和多肽，包括抗体。

13. 发明授权

US4261974A Valproic acid immunogen conjugates and antibodies thereto 失效
标题翻译：丙戊酸免疫原缀合物及其抗体
公开(公告)号：US4261974A
公开(公告)日：1981-04-14
申请号：US93372
申请日：1979-11-13
申请人： Robert T. Buckler , John F. Burd , Raphael C. Wong
发明人： Robert T. Buckler , John F. Burd , Raphael C. Wong
IPC分类号： G01N33/53 , A61K39/00 , C07H17/07 , C07H17/075 , C07K14/00 , C07K14/76 , C07K16/00 , C07K16/44 , G01N33/94 , A61K39/395 , C07C103/52
CPC分类号： C07K16/44 , C07H17/07 , G01N33/9473 , Y10S530/806
摘要： Reagents for use in binding assays, particularly immunoassays, to determine valproic acid in liquid media such as serum. Such reagents include antibody to valproic acid and .beta.-galactosyl-umbelliferone-valproic acid conjugates. Also provided are compounds used to prepare such reagents, including valproic acid immunogen conjugates and intermediates in the synthesis of such immunogen conjugates and .beta.-galactosyl-umbelliferone-labeled conjugates.
摘要翻译：用于结合测定法的试剂，特别是免疫测定法，用于测定液体培养基如血清中的丙戊酸。这些试剂包括丙戊酸抗体和β-半乳糖基 - 伞形酮 - 丙戊酸缀合物。还提供了用于制备此类试剂的化合物，包括丙戊酸免疫原缀合物和合成此类免疫原缀合物和β-半乳糖基 - 伞形酮标记的缀合物的中间体。

14. 发明授权

US4226978A .beta.-Galactosyl-umbelliferone-labeled aminoglycoside antibiotics and intermediates in their preparation 失效
标题翻译： β-半乳糖苷 - 伞形酮 - 标记的氨基糖苷类抗生素及其制备中间体
公开(公告)号：US4226978A
公开(公告)日：1980-10-07
申请号：US886094
申请日：1978-03-13
申请人： Robert C. Boguslaski , Robert J. Carrico , John F. Burd
发明人： Robert C. Boguslaski , Robert J. Carrico , John F. Burd
IPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , C07H21/00 , G01N33/533 , G01N33/535 , C07H15/22 , C07H17/04
CPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , C07H21/00 , G01N33/533 , G01N33/535
摘要： .beta.-Galactosyl-umbelliferone labeled aminoglycoside antibiotics, e.g., gentamicin, useful in homogeneous and heterogeneous specific binding assays for such antibiotics in liquid media such as serum. Also provided are a .beta.-galactosyl-umbelliferone-carboxylic acid and salts thereof which are intermediates in a method of preparing the labeled antibiotics.
摘要翻译： β-半乳糖基 - 伞形酮酮标记的氨基糖苷类抗生素，例如庆大霉素，可用于在诸如血清的液体培养基中的这种抗生素的均相和异质特异性结合测定。还提供了β-半乳糖基 - 伞形酮 - 羧酸及其盐，其是制备标记抗生素的方法中的中间体。

15. 发明授权

US4194048A Diphenylhydantoin derivatives 失效
标题翻译：二苯乙内酰脲衍生物
公开(公告)号：US4194048A
公开(公告)日：1980-03-18
申请号：US967131
申请日：1978-12-07
申请人： Robert T. Buckler
发明人： Robert T. Buckler
IPC分类号： A61K47/48 , C07D233/72 , C07D233/74 , C07D233/78 , C07H17/075 , G01N33/531 , G01N33/535
CPC分类号： A61K47/48192 , C07D233/72 , C07H17/075 , G01N33/531 , G01N33/535 , Y10S436/815 , Y10S436/823
摘要： Reagents for use in binding assays, particularly immuno-assays, to determine diphenylhydantoin and for preparing reagents employed in such assays, including .beta.-galactosyl-umbelliferone-diphenylhydantoin conjugates, diphenylhydantoin immunogens, and N.sup.1, N.sup.3 and .omicron.-phenyl derivatives of diphenylhydantoin for preparing same. N.sup.1 and N.sup.3 -.omega.-aminoalkyl and .omicron.-(.omega.-aminoalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to a .beta.-galactosyl-umbelliferone derivative to form labeled conjugates useful in homogeneous or heterogeneous binding assays. N.sup.1 and N.sup.3 -.omega.-carboxyalkyl and .omicron.-(.omega.-carboxyalkoxy)-phenyl derivatives are prepared and coupled by amide linkage to an immunogenic polyamino acid to form immunogen conjugates against which diphenylhydantoin-specific antibodies can be raised.
摘要翻译：用于结合测定的试剂，特别是免疫测定法，用于测定二苯乙内酰脲和用于制备此类测定中使用的试剂，包括β-半乳糖基 - 伞形酮 - 二苯乙内酰脲共轭物，二苯乙内酰脲免疫原和二苯乙内酰脲的N1，N3和二 - 相同。制备N1和N3-ω-氨基烷基和亚氨基 - （ω-氨基烷氧基） - 苯基衍生物，并通过酰胺键与β-半乳糖基 - 伞形酮衍生物偶联以形成用于均相或异质结合测定的标记共轭物。制备N1和N3-ω-羧基烷基和二（ω-羧甲烷氧基） - 苯基衍生物，并通过酰胺键与免疫原性聚氨基酸偶联，形成可以提高二苯乙内酰脲特异性抗体的免疫原缀合物。

16. 发明授权

US4147704A Derivatives of novobiocin 失效
标题翻译：新生霉素衍生物
公开(公告)号：US4147704A
公开(公告)日：1979-04-03
申请号：US878117
申请日：1978-02-15
申请人： Lester A. Dolak
发明人： Lester A. Dolak
IPC分类号： C07D311/16 , C07H17/075 , C07D311/56
CPC分类号： C07H17/075
摘要： The present invention relates to processes for the chemical oxidation of novobiocin-type compounds (I) to their corresponding hydroxy derivative (II) or aldehyde (III) and formation of novobiocin derivatives from these oxidation products. The oxidation products and derivatives have antibacterial activity.
摘要翻译：本发明涉及将新生霉素型化合物（I）化学氧化成其相应的羟基衍生物（II）或醛（III）并从这些氧化产物形成新生霉素衍生物的方法。氧化产物和衍生物具有抗菌活性。

17. 发明授权

US3573275A Isolation and purification of coumermycin antibiotics 失效
标题翻译：氯霉素抗生素的分离和纯化
公开(公告)号：US3573275A
公开(公告)日：1971-03-30
申请号：US3573275D
申请日：1969-02-20
申请人： HOFFMANN LA ROCHE
发明人： MAEHR HUBERT , SCANNELL JAMES PARNELL , ZEITZ VERNON
IPC分类号： C07H17/075 , C07C47/18
CPC分类号： C07H17/075
摘要： A CRUDE ANTIBIOTIC COMPLEX KNOWN AS THE "COUMERMYCIN COMPLEX" IS EXTRACTED FROM THE FERMENTATION BROTH OR CELLS WITH A WATER MISCIBLE ORGANIC SOLVENT IN A BASIC OR SLIGHTLY ACIDIC MEDIUM. THEREAFTER, THE CRUDE COMPLEX IS ISOLATED BY ACIDIFICATION AND FILTRATION.

18. 发明授权

US3380994A Coumermycin derivatives 失效
标题翻译：香豆素衍生物
公开(公告)号：US3380994A
公开(公告)日：1968-04-30
申请号：US58399266
申请日：1966-10-03
申请人： BRISTOL MYERS CO
发明人： NETTLETON JR DONALD EDWARD
IPC分类号： C07H17/075
CPC分类号： C07H17/075

19. 发明授权

US3268511A 5, 5-diloweralkyl-4-omicron-lower alkyl-2, 3-cyclocarbonato-l-pyranosyl halides 失效
标题翻译： 5,5-二氟烷基-4-微米 - 低级烷基-2,3-环状羰基-1-吡喃糖基卤化物
公开(公告)号：US3268511A
公开(公告)日：1966-08-23
申请号：US15020361
申请日：1961-11-06
申请人： HOFFMANN LA ROCHE
发明人： JOSEPH KISS , HANS SPIEGELBERG , PETER VATERLAUS BRUNO , ISRAEL RACHLIN ALBERT , KARL DOEBEL
IPC分类号： C07H17/07 , C07H17/075 , C07H19/01
CPC分类号： C07H17/075 , C07H17/07 , C07H19/01

20. 发明授权

US3184887A Process of producing glycosides from living plants 失效
标题翻译：从活植物生产糖苷的方法
公开(公告)号：US3184887A
公开(公告)日：1965-05-25
申请号：US10161761
申请日：1961-03-14
申请人： MADAUS & CO KOMMANDITGESELLSCH
发明人： ILSE WINTER
IPC分类号： C07H1/08 , C07H17/075
CPC分类号： C07H17/075 , C07H1/08

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