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11. 发明申请

US20120164643A1 SET OF OLIGONUCLEOTIDE PROBES AS WELL AS METHODS AND USES THERETO 有权
标题翻译：作为方法和用途的一组寡核苷酸探针
公开(公告)号：US20120164643A1
公开(公告)日：2012-06-28
申请号：US13361549
申请日：2012-01-30
申请人： Frank Bergmann , Walter Eberle , Thomas Fischer , Herbert von der Eltz
发明人： Frank Bergmann , Walter Eberle , Thomas Fischer , Herbert von der Eltz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译：本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。

12. 发明申请

US20120157327A1 Labelling Strategies for the Sensitive Detection of Analytes 审中-公开
公开(公告)号：US20120157327A1
公开(公告)日：2012-06-21
申请号：US13411021
申请日：2012-03-02
申请人： Thomas CARELL , Anja SCHWÕEGLER , Glenn A. BURLEY , Johannes GIERLICH , Mohammad Reza MOFID
发明人： Thomas CARELL , Anja SCHWÕEGLER , Glenn A. BURLEY , Johannes GIERLICH , Mohammad Reza MOFID
IPC分类号： C12Q1/68 , G01N33/53 , G01N21/75 , C12P19/34 , C40B30/00 , C07H21/04 , C07H21/02 , C07H1/00 , G01N33/58 , G01N27/00
CPC分类号： G01N33/5308 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6818 , Y10T436/143333 , C12Q2565/1025
摘要： The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological samples.

13. 发明授权

US08148512B2 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US08148512B2
公开(公告)日：2012-04-03
申请号：US12819101
申请日：2010-06-18
申请人： Krassen M. Dimitrov
发明人： Krassen M. Dimitrov
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of antigenedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了多种独特标记的探针，其含有目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗原多肽杂交的条件下使混合物接触，所述多个抗Genetigits具有足以独特地与指定剂内的genesigits杂交的多样性，以及（c）唯一地检测杂交的混合物中一种或多种分析物之间的复合物，目标特异性探针和抗Genetigigit。

14. 发明申请

US20110223592A1 Multiple fluorophore detector system 有权
标题翻译：多重荧光团检测系统
公开(公告)号：US20110223592A1
公开(公告)日：2011-09-15
申请号：US11785441
申请日：2007-04-18
申请人： Matthew P. Collis
发明人： Matthew P. Collis
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6816 , C12Q2565/1025
摘要： A detector oligonucleotide comprises multiple pairs of a donor fluorophore and a quencher molecule, which donor fluorophores and quencher molecules are separated by a site that is capable of being cleaved when in double-stranded form. The detector oligonucleotide may be made double-stranded in a manner that depends on the presence of a target nucleic acid, allowing the cleavage sites to be cleaved. Separation of the donor fluorophores and the quencher molecules decreases fluorescence quenching and generates a detectable change in a fluorescence parameter of the fluorophores of the detector oligonucleotide. By using multiple donor/quencher pairs, the present detector oligonucleotide advantageously generates a high signal to noise ratio and high efficiency in detection of a target nucleic acid.
摘要翻译：检测器寡核苷酸包含多对供体荧光团和猝灭剂分子，供体荧光团和猝灭剂分子由能双链形式切割的位点分开。检测器寡核苷酸可以以取决于靶核酸的存在的方式制成双链，允许切割位点被切割。供体荧光团和猝灭剂分子的分离会降低荧光猝灭，并产生检测器寡核苷酸的荧光团的荧光参数的可检测变化。通过使用多个供体/猝灭剂对，本发明的检测器寡核苷酸有利地产生高的信噪比并且在靶核酸的检测中具有高效率。

15. 发明申请

US20100240103A1 OLIGONUCLEOTIDES LABELED WITH A PLURALITY OF FLUOROPHORES 审中-公开
标题翻译：标有多种荧光素的寡核苷酸
公开(公告)号：US20100240103A1
公开(公告)日：2010-09-23
申请号：US12646514
申请日：2009-12-23
申请人： Fei Mao , Xing Xin
发明人： Fei Mao , Xing Xin
IPC分类号： C12P19/34 , C07H21/00
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y10S435/975 , C12Q2565/1015 , C12Q2561/101 , C12Q2565/1025
摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5′-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.
摘要翻译：本发明的一个实施方案公开了通过用多个光谱相同或相似的染料和任选地与一种或多种猝灭剂染料标记寡核苷酸来设计标记的核酸探针和引物的新方法。根据本发明的一些实施方案标记的寡核苷酸在标记染料彼此分离时表现出可检测的信号增加，例如荧光信号。用于分离染料的方法包括切割标记的寡核苷酸包括使用具有5'-外切核酸酶活性的酶。在一个实施方案中，本发明的核酸引物可以在与靶序列杂交时发荧光，并掺入扩增产物。本发明的核酸探针和引物具有广泛的应用范围，从靶核酸序列的一般检测到临床诊断。包括本发明许多实施方案的核酸探针和引物的寡核苷酸的主要优点是它们的合成简便性，光谱通用性和优异的荧光信号。

16. 发明申请

US20100203545A1 Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs) 审中-公开
标题翻译：微RNA的两色实时/终点定量（miRNA）
公开(公告)号：US20100203545A1
公开(公告)日：2010-08-12
申请号：US12760524
申请日：2010-04-14
申请人： Andrew K. Finn , Caifu Chen
发明人： Andrew K. Finn , Caifu Chen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q2565/1025 , C12Q2537/143 , C12Q2525/161 , C12Q2525/301
摘要： The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于在两个样品之间检测靶多核苷酸序列，特别是小目标多核苷酸如miRNA的方法，试剂，试剂盒和组合物。可以在两个不同的反应中使用一对接头探针来查询特定种类的靶多核苷酸。可以在扩增反应中使用一对检测器探针，针对靶多核苷酸特异性的单一正向引物和反向引物来查询两个样品之间的靶多核苷酸表达水平的差异。在一些实施方案中，使用多个接头探针查询多个小miRNA。然后可以在多个扩增反应中解码多个查询的miRNA。

17. 发明申请

US20100136531A1 NUCLEIC ACID DETECTION USING LATERAL FLOW METHODS 审中-公开
标题翻译：使用侧向流动法进行核酸检测
公开(公告)号：US20100136531A1
公开(公告)日：2010-06-03
申请号：US12296536
申请日：2007-04-10
申请人： Ian Garthwaite , Philip A. Myers , Christine M. Sadek
发明人： Ian Garthwaite , Philip A. Myers , Christine M. Sadek
IPC分类号： C12Q1/68
CPC分类号： G01N33/56911 , C12Q1/6804 , C12Q1/6816 , G01N33/5308 , G01N33/558 , G01N33/585 , Y02A50/451 , C12Q2565/518 , C12Q2565/1025 , C12Q2545/101 , C12Q2563/155 , C12Q2563/137 , C12Q2563/131 , C12Q2565/625
摘要： Methods and kits for use in detecting a target nucleic acid in a sample are disclosed. In one particular application, the methods and kits allow for the detection of an undesirable micro-organism (e.g. Listeria, Salmonella or Enterobacteriaceae) in food or present on a food preparation surface.
摘要翻译：公开了用于检测样品中靶核酸的方法和试剂盒。在一个特定应用中，所述方法和试剂盒允许在食物中检测不期望的微生物（例如李斯特菌，沙门氏菌或肠杆菌科）或存在于食物制剂表面上。

18. 发明申请

US20090215643A1 Highly Visible Chromosome-Specific Probes and Related Methods 审中-公开
标题翻译：高可见染色体特异性探针及相关方法
公开(公告)号：US20090215643A1
公开(公告)日：2009-08-27
申请号：US12179770
申请日：2008-07-25
申请人： Joan Aurich-Costa , Sean P. Bradley , Phillip T. Moen, JR.
发明人： Joan Aurich-Costa , Sean P. Bradley , Phillip T. Moen, JR.
IPC分类号： C40B30/04 , C07H21/00 , G01N21/76 , C40B40/06 , G01N33/48 , C12Q1/68 , C07H1/00
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/682 , C12Q1/6841 , C12Q2600/156 , Y10T436/143333 , C12Q2525/301 , C12Q2563/107 , C12Q2565/1025 , C12Q2525/185
摘要： Disclosed are chromosome-specific synthetic oligonucleotides and labeled probe compositions, as well as related methods for preparing and using such compositions. Also disclosed are kits for utilization in methods for preparing or using the labeled probes.
摘要翻译：公开了染色体特异性合成寡核苷酸和标记的探针组合物，以及制备和使用这些组合物的相关方法。还公开了用于制备或使用标记探针的方法中的试剂盒。

19. 发明申请

US20060199216A1 Programmable molecular barcodes 审中-公开
标题翻译：可编程分子条形码
公开(公告)号：US20060199216A1
公开(公告)日：2006-09-07
申请号：US11430590
申请日：2006-05-08
申请人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
发明人： Xing Su , Tae-Woong Koo , Andrew Berlin , Lei Sun , Narayanan Sundararajan , Mineo Yamakawa
IPC分类号： C12Q1/68 , G01N33/551
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.
摘要翻译：本公开涉及用于生产和/或使用分子条形码的方法。在本发明的某些实施方案中，条形码包含可包含一个或多个分支结构的聚合物主链。标签可以附加到骨干和/或分支结构。条形码还可以包含可以与靶标结合的探针，例如蛋白质，核酸和其他生物分子或聚集体。可以通过标签的类型和位置区分不同的条形码。在其它实施方案中，条形码可以通过将一个或多个标记的寡核苷酸与包含容器部分和探针部分的模板杂交来产生。标记的寡核苷酸可以被设计为模块代码部分，以形成针对不同靶标的不同条形码。在替代实施例中，条形码可以通过单体单元的聚合来制备。绑定的条形码可以通过各种成像模式来检测，例如表面等离子体共振，荧光或拉曼光谱。

20. 发明申请

US20050233332A1 Multiple fluorophore detector system 审中-公开
标题翻译：多重荧光团检测系统
公开(公告)号：US20050233332A1
公开(公告)日：2005-10-20
申请号：US10825943
申请日：2004-04-14
申请人： Matthew Collis
发明人： Matthew Collis
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6816 , C12Q2565/1025
摘要： A detector oligonucleotide comprises multiple pairs of a donor fluorophore and a quencher molecule, which donor fluorophores and quencher molecules are separated by a site that is capable of being cleaved when in double-stranded form. The detector oligonucleotide may be made double-stranded in a manner that depends on the presence of a target nucleic acid, allowing the cleavage sites to be cleaved. Separation of the donor fluorophores and the quencher molecules decreases fluorescence quenching and generates a detectable change in a fluorescence parameter of the fluorophores of the detector oligonucleotide. By using multiple donor/quencher pairs, the present detector oligonucleotide advantageously generates a high signal to noise ratio and high efficiency in detection of a target nucleic acid.
摘要翻译：检测器寡核苷酸包含多对供体荧光团和猝灭剂分子，供体荧光团和猝灭剂分子由能双链形式切割的位点分开。检测器寡核苷酸可以以取决于靶核酸的存在的方式制成双链，允许切割位点被切割。供体荧光团和猝灭剂分子的分离会降低荧光猝灭，并产生检测器寡核苷酸的荧光团的荧光参数的可检测变化。通过使用多个供体/猝灭剂对，本发明的检测器寡核苷酸有利地产生高的信噪比并且在靶核酸的检测中具有高效率。

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