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11. 发明授权

US5283122A Fused liposome and acid induced method for liposome fusion 失效
标题翻译：融合脂质体和酸诱导的脂质体融合方法
公开(公告)号：US5283122A
公开(公告)日：1994-02-01
申请号：US836125
申请日：1992-02-14
申请人： Leaf Huang , Jerome Connor
发明人： Leaf Huang , Jerome Connor
IPC分类号： A61K9/127 , C12N15/88
CPC分类号： A61K9/1272 , C12N15/88 , Y10S436/829 , Y10T428/2984
摘要： Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulated calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.
摘要翻译：含有磷脂酰乙醇胺，棕榈酰高半胱氨酸或油酸或棕榈酸的脂质体当培养基的pH值降至7以下时迅速融合。脂质体融合通过（a）如共振能量转移所示脂质体脂质的混合，（b）凝胶过滤和（c）电子显微镜。磷脂酰乙醇胺或其酸加成酯在脂质体中的存在大大增强了融合; 而磷脂酰胆碱的存在抑制融合。在含有磷脂酰乙醇胺的脂质体融合过程中，棕榈酰同型半胱氨酸（8：2）几乎全部被包裹的钙黄绿素释放出来。在脂质体中包含胆固醇（40％）可显着减少渗漏而不损害融合。将这些pH敏感的脂质体融合以将生物活性分子例如DNA递送到活细胞中。

12. 发明授权

US4789633A Fused liposome and acid induced method for liposome fusion 失效
标题翻译：融合脂质体和酸诱导的脂质体融合方法
公开(公告)号：US4789633A
公开(公告)日：1988-12-06
申请号：US602177
申请日：1984-04-19
申请人： Leaf Huang , Jerome Connor
发明人： Leaf Huang , Jerome Connor
IPC分类号： A01H1/00 , A01H5/00 , A61K9/00 , A61K9/127 , B01J13/02 , C12N5/00 , C12N5/02 , C12N5/10 , C12N15/00 , C12N15/88 , A61J5/00 , B32B5/16
CPC分类号： A61K9/127 , A61K9/1272 , C12N15/88 , Y10T428/2984
摘要： Liposomes containing phosphatidylethanolamine, palmitoyl homocysteine or oleic acid or palmitic acid, fuse rapidly when the pH of the medium is reduced below 7. Liposome fusion was measured by (a) mixing of the liposomal lipids as shown by resonance energy transfer, (b) gel filtration and (c) electron microscopy. The presence of phosphatidylethanolamine or acid addition esters thereof in the liposomes greatly enhances fusion; whereas the presence of phosphatidylcholine inhibits fusion. During fusion of liposomes containing phosphatidylethanolamine:palmitoyl homocysteine (8:2), almost all of the encapsulate calcein is released. Inclusion of cholesterol (40%) in the liposomes substantially decreases leakage without impairing fusion. Those pH sensitive liposomes are fused to deliver biologically active molecules such as DNA, into living cells.
摘要翻译：含有磷脂酰乙醇胺，棕榈酰高半胱氨酸或油酸或棕榈酸的脂质体当培养基的pH值降至7以下时迅速融合。脂质体融合通过（a）如共振能量转移所示脂质体脂质的混合，（b）凝胶过滤和（c）电子显微镜。磷脂酰乙醇胺或其酸加成酯在脂质体中的存在大大增强了融合; 而磷脂酰胆碱的存在抑制融合。在含有磷脂酰乙醇胺的脂质体融合过程中，棕榈酰同型半胱氨酸（8：2）几乎全部释放出钙黄绿素。在脂质体中包含胆固醇（40％）可显着减少渗漏而不损害融合。将这些pH敏感的脂质体融合以将生物活性分子例如DNA递送到活细胞中。

13. 发明申请

US20120141974A1 CRYOPRESERVATION OF HUMAN RED BLOOD CELLS 有权
标题翻译：人红细胞的CRYOPRESERVATION
公开(公告)号：US20120141974A1
公开(公告)日：2012-06-07
申请号：US13368460
申请日：2012-02-08
申请人： Stephen A. Livesey , Michael Brian Burnett , Jerome Connor , Christopher Todd Wagner
发明人： Stephen A. Livesey , Michael Brian Burnett , Jerome Connor , Christopher Todd Wagner
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0226 , A61K35/18
摘要： A red blood cell storage composition includes a composition of red blood cells and biochemistry altering reagents, the biochemistry altering reagents being present at a concentration so as to reduce the percent hemolysis of the red blood cells during the freeze-thaw cycle below that of the percent hemolysis of the red blood cells in the absence the biochemistry altering reagents. The red blood cell storage composition preferably includes reagents selected from: modifiers of glycolytic/metabolic components, modifiers of antioxidant potential, effectors of intracellular ionic distribution, modifiers of membrane fluidity, modifiers of cytoskeletal structure, effectors of the cyclooxygenase second messenger pathway, effectors of the lipoxygenase second messenger pathway, effectors of the hexose monophosphate second messenger pathway, effectors of the phosphorylation second messenger pathway, modifiers of specific messenger molecules, and combinations thereof.
摘要翻译：红细胞储存组合物包括红细胞和生物化学改变试剂的组合物，所述生物化学改变试剂以浓度存在，以便将冻融循环期间的红细胞溶血百分比降低至百分比以下在没有生物化学改变试剂的情况下红细胞溶血。红细胞储存组合物优选包括选自以下的试剂：糖酵解/代谢组分的改性剂，抗氧化剂潜力的改性剂，细胞内离子分布的作用剂，膜流动性的调节剂，细胞骨架结构的改性剂，环加氧酶第二信使途径的作用者，脂氧合酶第二信使通路，己糖单磷酸第二信使途径的作用子，磷酸化第二信使途径的作用剂，特异信使分子的改性剂及其组合。

14. 发明授权

US5622867A Prolonged preservation of blood platelets 失效
标题翻译：长时间保存血小板
公开(公告)号：US5622867A
公开(公告)日：1997-04-22
申请号：US326036
申请日：1994-10-19
申请人： Stephen A. Livesey , Jerome Connor , Laura M. Currie
发明人： Stephen A. Livesey , Jerome Connor , Laura M. Currie
IPC分类号： A01N1/02 , A61K35/14 , A61K35/16 , A61K35/19
CPC分类号： A01N1/02 , A01N1/0226 , A61K35/19 , Y10T436/108331 , Y10T436/2525
摘要： This invention provides a method for prolonging the preservation of human blood platelets at reduced temperatures. The method uses an inhibitor system that enables blood platelets to maintain their discoid shape and retain their functional integrity during storage. This is accomplished by interrupting normal platelet function during storage, so as to help keep platelets from activating and losing their shape. Before using the platelets in a transfusion, they are returned to their normal functional level by washing the inhibitor system away from the platelets.
摘要翻译：本发明提供了一种在降低的温度下延长人血小板保存的方法。该方法使用抑制剂系统，其使血小板维持其盘状并在储存期间保持其功能完整性。这是通过在储存期间中断正常的血小板功能来实现的，以便有助于保持血小板活化和失去其形状。在使用血小板输血之前，通过将抑制剂系统从血小板上清洗而使其恢复到正常的功能水平。

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