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11. 发明授权

US07833524B2 Method of preparing botulinum neurotoxin type E light chain 有权
标题翻译：制备肉毒杆菌神经毒素E型轻链的方法
公开(公告)号：US07833524B2
公开(公告)日：2010-11-16
申请号：US12273157
申请日：2008-11-18
申请人： Eric A. Johnson , Marite Bradshaw , Michael Baldwin , Joseph T. Barbieri
发明人： Eric A. Johnson , Marite Bradshaw , Michael Baldwin , Joseph T. Barbieri
IPC分类号： C12P1/04
CPC分类号： C07K14/33
摘要： The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.
摘要翻译：本发明提供了A型或E型轻链型肉毒杆菌毒素的制备方法，其中所述制剂既具有催化活性，又具有可溶性。优选地，制剂基本上由A型肉毒毒素轻链的氨基酸残基1至425组成。还提供了筛选抑制剂的方法，其中所述方法包括将测试抑制剂暴露于A型轻链A型肉毒杆菌毒素的制备评估制剂的生物活性。在另一个实施方案中，提供了提供A型肉毒毒素轻链的催化活性可溶性制剂的方法，其中所述方法包括获得包含编码氨基酸残基1-425的DNA序列并表达多肽的表达载体。

12. 发明申请

US20080063867A1 Method and apparatus associated with anisotropic shrink in sintered ceramic items 有权
标题翻译：烧结陶瓷制品各向异性收缩的方法和装置
公开(公告)号：US20080063867A1
公开(公告)日：2008-03-13
申请号：US11788286
申请日：2007-04-19
申请人： M. Schlienger , Nina Bergan French , Michael Baldwin , Michael Maguire , Paul Withey
发明人： M. Schlienger , Nina Bergan French , Michael Baldwin , Michael Maguire , Paul Withey
IPC分类号： B29C35/08 , C04B33/02
CPC分类号： B28B1/001 , B28B1/00 , B29C64/106 , B29C64/135 , B33Y10/00 , B33Y30/00 , B33Y50/02 , B33Y70/00 , B33Y80/00 , C04B35/111 , C04B35/6263 , C04B35/6269 , C04B35/63 , C04B35/632 , C04B38/00 , C04B38/0022 , C04B2111/00181 , C04B2201/20 , C04B2235/5436 , C04B2235/5445 , C04B2235/77 , C04B2235/9615 , Y10T428/2982 , C04B35/10
摘要： A manufacturing method for producing ceramic item from a photocurable ceramic filled material by stereolithography. The method compensates for the anisotropic shrinkage of the item during firing to produce a dimensionally accurate item.
摘要翻译：通过立体光刻法从可光固化陶瓷填充材料制造陶瓷制品的制造方法。该方法在烧制期间补偿物品的各向异性收缩以产生尺寸准确的物品。

13. 发明申请

US20060118266A1 Method and apparatus for production of a cast component 有权
公开(公告)号：US20060118266A1
公开(公告)日：2006-06-08
申请号：US11209389
申请日：2005-08-23
申请人： Donald Frasier , Matthew Kush , Max Schlienger , Michael Baldwin
发明人： Donald Frasier , Matthew Kush , Max Schlienger , Michael Baldwin
IPC分类号： B22D27/04 , B22D35/00
CPC分类号： B29C67/0092 , B22C9/02 , B22C9/04 , B22C9/22 , B22D27/045 , B22D35/04 , B22D39/06 , B22D43/001 , B29C64/40 , B33Y80/00 , C30B11/00 , C30B11/002 , C30B11/14 , C30B29/52 , G03F7/0037 , G03F7/0047 , Y02P10/292
摘要： A system for producing cast components from molten metal. One form of the present invention includes a system for the precision pouring of molten metal within a casting mold.

14. 发明授权

US07465457B2 Method for preparing botulinum neurotoxin type A light chain 有权
标题翻译：制备肉毒杆菌神经毒素A型轻链的方法
公开(公告)号：US07465457B2
公开(公告)日：2008-12-16
申请号：US11404289
申请日：2006-04-14
申请人： Eric A. Johnson , Marite Bradshaw , Michael Baldwin , Joseph T. Barbieri
发明人： Eric A. Johnson , Marite Bradshaw , Michael Baldwin , Joseph T. Barbieri
IPC分类号： A61K39/08 , C12P21/02
CPC分类号： C07K14/33
摘要： The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.
摘要翻译：本发明提供了A型或E型轻链型肉毒杆菌毒素的制备方法，其中所述制剂既具有催化活性，又具有可溶性。优选地，制剂基本上由A型肉毒毒素轻链的氨基酸残基1至425组成。还提供了筛选抑制剂的方法，其中所述方法包括将测试抑制剂暴露于A型轻链A型肉毒杆菌毒素的制备评估制剂的生物活性。在另一个实施方案中，提供了提供A型肉毒毒素轻链的催化活性可溶性制剂的方法，其中所述方法包括获得包含编码氨基酸残基1-425的DNA序列并表达多肽的表达载体。

15. 发明申请

US20070196683A1 Method for casting reactive metals and casting containers associated therewith 审中-公开
标题翻译：铸造与之相关的活性金属和铸造容器的方法
公开(公告)号：US20070196683A1
公开(公告)日：2007-08-23
申请号：US11653739
申请日：2007-01-16
申请人： Max Schlienger , Michael Baldwin , Michael Maguire
发明人： Max Schlienger , Michael Baldwin , Michael Maguire
IPC分类号： B05D3/04 , B05D3/02 , B05D1/18 , B32B3/10
CPC分类号： B22C9/02 , B22C9/00 , B22C9/10 , B22D21/005 , B22D21/02 , B22D21/022 , B22D27/00 , B22D41/02 , B28B1/00 , B28B1/001 , B28B7/346 , C04B35/111 , C04B35/185 , C04B35/486 , C04B35/505 , C04B2235/5445 , C04B2235/72 , C22B34/1295 , Y02P10/292 , Y10T428/12361 , Y10T428/12806 , Y10T428/24273
摘要： An investment casting system for producing a cast article from a reactive metal that is free of an undesirable surface layer generally associated with the reaction between the molten metal and the casting mold. In one form the casting mold is substantially free of silica. A silica free ceramic crucible for heating and holding molten reactive metal is also contemplated herein.
摘要翻译：一种用于从反应性金属生产铸造制品的熔模铸造系统，其不具有通常与熔融金属和铸模之间的反应相关联的不期望的表面层。在一种形式中，铸模基本上不含二氧化硅。本发明也考虑了一种用于加热和保持熔融反应性金属的无二氧化硅陶瓷坩埚。

16. 发明申请

US20070059326A1 Escherichia coli-derived vaccine and therapy against botulism 审中-公开
标题翻译：大肠杆菌来源的疫苗和针对肉毒中毒的治疗
公开(公告)号：US20070059326A1
公开(公告)日：2007-03-15
申请号：US11289851
申请日：2005-11-30
申请人： Michael Baldwin , Marite Bradshaw , William Tepp , Eric Johnson , Joseph Barbieri , Christina Pier
发明人： Michael Baldwin , Marite Bradshaw , William Tepp , Eric Johnson , Joseph Barbieri , Christina Pier
IPC分类号： A61K39/08 , C07H21/04 , C12N15/74 , C12N1/21 , C07K14/33
CPC分类号： C12N9/52 , A61K39/0258 , A61K39/08 , A61K2039/523 , C12Y304/24069 , Y02A50/469 , Y02A50/474
摘要： A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparing E. coli transformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.
摘要翻译：公开了产生肉毒杆菌毒素C末端受体结合结构域（HCR）的方法。该方法包括以下步骤：（a）用包含编码HCR蛋白的DNA的表达载体转化大肠杆菌，（b）在培养基中降低温度诱导HCR蛋白的表达，和（c）通过提取纯化HCR蛋白质，其中提取物通过离心和过滤进行澄清，其中纯化的HCR蛋白质为至少10mg / L的培养基。

17. 发明申请

US20060258847A1 Method of preparing botulinum neurotoxin type A light chain 有权
标题翻译：制备肉毒杆菌神经毒素A型轻链的方法
公开(公告)号：US20060258847A1
公开(公告)日：2006-11-16
申请号：US11404289
申请日：2006-04-14
申请人： Eric Johnson , Marite Bradshaw , Michael Baldwin , Joseph Barbieri
发明人： Eric Johnson , Marite Bradshaw , Michael Baldwin , Joseph Barbieri
IPC分类号： C07K14/00 , C07K1/00 , C07K17/00
CPC分类号： C07K14/33
摘要： The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.
摘要翻译：本发明提供了A型或E型轻链型肉毒杆菌毒素的制备方法，其中所述制剂既具有催化活性，又具有可溶性。优选地，制剂基本上由A型肉毒毒素轻链的氨基酸残基1至425组成。还提供了筛选抑制剂的方法，其中所述方法包括将测试抑制剂暴露于A型轻链A型肉毒杆菌毒素的制备评估制剂的生物活性。在另一个实施方案中，提供了提供A型肉毒毒素轻链的催化活性可溶性制剂的方法，其中所述方法包括获得包含编码氨基酸残基1〜425的DNA序列并表达多肽的表达载体。

18. 发明申请

US20050050548A1 Application internationalization using dynamic proxies 审中-公开
标题翻译：使用动态代理的应用程序国际化
公开(公告)号：US20050050548A1
公开(公告)日：2005-03-03
申请号：US10650880
申请日：2003-08-28
申请人： Joseph Sheinis , Michael Baldwin , Alexander Sherkin
发明人： Joseph Sheinis , Michael Baldwin , Alexander Sherkin
IPC分类号： G06F9/00 , G06F9/42 , G06F9/44
CPC分类号： G06F9/4488 , G06F9/4486 , G06F9/454 , G06F2209/542
摘要： An application that was not internationalized when coded may be internationalized through the addition of interception and localization logic and tables without modification of the original application logic. The interception logic may be configured to intercept calls to an application component and invoke localization logic in response to an intercepted call to the application component. The interception logic may use dynamic proxies to intercept method calls from a client component to an application component both before and after the execution of the method. The interception logic may use JAVA reflection to determine whether input parameters or return values associated with the method call are localizable. The application component logic may operate on data stored in a primary database table in which the data is represented in the system default locale.
摘要翻译：编码时未国际化的应用程序可以通过添加拦截和本地化逻辑和表来进行国际化，而不会修改原始应用程序逻辑。截取逻辑可以被配置为截取对应用组件的调用并且响应于被截取的对应用组件的调用来调用定位逻辑。截取逻辑可以使用动态代理在方法执行之前和之后拦截从客户端组件到应用程序组件的方法调用。拦截逻辑可以使用JAVA反射来确定与方法调用相关联的输入参数或返回值是否可本地化。应用程序组件逻辑可以对存储在主数据库表中的数据进行操作，其中数据在系统默认语言环境中被表示。

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