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11. 发明申请

US20070158213A1 Dual Type Potentiometric Creatinine Biosensor 失效
标题翻译：双电位电生理传感器
公开(公告)号：US20070158213A1
公开(公告)日：2007-07-12
申请号：US11457589
申请日：2006-07-14
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-Wei Pan , Nien-Hsuan Chou
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-Wei Pan , Nien-Hsuan Chou
IPC分类号： C12Q1/00 , G01F1/64
CPC分类号： C12Q1/002 , C12Q1/005 , G01N33/70
摘要： In this invention, a separative structure of the ion-selecting electrode is applied to fabricate the dual type potentiometric biosensor for creatinine detection. According to the fabrication process, the creatinine enzyme is immobilized onto a conductive layer of a substrate of pH sensing membrane and ammonium ion-selecting membrane. The conductive layer provides with a sensing region and a non-sensing region. A conductive line is extended from the conductive layer for using as an external electrical contact point. The dual type potentiometric creatinine biosensor was fabricated by using the enzyme immobilization method onto the surface of the selecting membrane of sensing membrane of pH sensing electrode and creatinine sensor.
摘要翻译：在本发明中，应用离子选择电极的分离结构来制造用于肌酸酐检测的双重电位生物传感器。根据制造方法，将肌酸酐酶固定在pH感应膜和铵离子选择膜的底物的导电层上。导电层提供感测区域和非感测区域。导电线从导电层延伸以用作外部电接触点。通过使用酶固定法在pH感应电极和肌酐传感器的感应膜选择膜的表面上制作双电位电位生物传感器。

12. 发明申请

US20060096858A1 Potentiometric urea sensor based on ion-selective electrode 审中-公开
标题翻译：基于离子选择电极的电位式尿素传感器
公开(公告)号：US20060096858A1
公开(公告)日：2006-05-11
申请号：US10984495
申请日：2004-11-08
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-We Pan , Nien-Hsuan Chou
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-We Pan , Nien-Hsuan Chou
IPC分类号： G01N33/487 , C12Q1/00
CPC分类号： C12Q1/005 , C12Q1/002
摘要： The present invention is a sensor for detecting urea using a separating-style structure of ion-selective electrode, in which an ammonium ion-selective membrane is immobilized on a conductive layer on the surface of a substrate, said conductive layer has a sensing region and a non-sensing region after packaged, and a conductive line is used to retrieve a sensing signal from said conductive layer. In the end, employing the enzyme immobilization method to immobilize a urea enzyme onto ammonium ion-selective membrane, thus the fabrication of a potentiometric urea sensor is completed.
摘要翻译：本发明是一种使用离子选择性电极的分离型结构检测尿素的传感器，其中铵离子选择性膜固定在基板表面上的导电层上，所述导电层具有感测区域和封装后的非感测区域和导线用于从所述导电层检索感测信号。最后，采用酶固定方法将尿素酶固定在铵离子选择性膜上，从而完成电位尿素传感器的制造。

13. 发明申请

US20100025265A1 Potentiometric biosensor and the forming method thereof 审中-公开
标题翻译：电位生物传感器及其形成方法
公开(公告)号：US20100025265A1
公开(公告)日：2010-02-04
申请号：US12372915
申请日：2009-02-18
申请人： Shen-Kan Hsiung , Nien-Hsuan Chou , Jung-Chuan Chou , Tai-Ping Sun
发明人： Shen-Kan Hsiung , Nien-Hsuan Chou , Jung-Chuan Chou , Tai-Ping Sun
IPC分类号： G01N27/26
CPC分类号： C12Q1/58 , C12Q1/001 , G01N27/3275 , G01N2333/978
摘要： The present invention discloses a potentiometric biosensor for urea and creatinine detection, and the forming method thereof. The disclosed biosensor comprises a substrate, at least two working electrode on the substrate, at least one reference electrode on the substrate, an internal reference electrode on the substrate, and a packaging structure which separates the adjacent electrodes. The working electrode comprises urease or creatinine iminohydrolase (CIH). The detection signal is transmitted for further processing through a wire or an exposed surface on the biosensor. The disclosed biosensor is replaceable.
摘要翻译：本发明公开了尿素和肌酐检测用电位生物传感器及其形成方法。所公开的生物传感器包括衬底，衬底上的至少两个工作电极，衬底上的至少一个参考电极，衬底上的内部参考电极以及分隔相邻电极的封装结构。工作电极包括尿素酶或肌酐亚氨基水解酶（CIH）。检测信号通过生物传感器上的线或暴露表面进行进一步处理。所公开的生物传感器是可更换的。

14. 发明申请

US20090194416A1 Potentiometric biosensor for detection of creatinine and forming method thereof 审中-公开
标题翻译：用于检测肌酐的电位生物传感器及其形成方法
公开(公告)号：US20090194416A1
公开(公告)日：2009-08-06
申请号：US12024066
申请日：2008-01-31
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou
IPC分类号： G01N27/327 , B05D5/12
CPC分类号： G01N33/70 , C12Q1/34 , G01N2333/986
摘要： The present invention discloses a potentiometric biosensor for detecting creatinine, and the forming method thereof. The disclosed biosensor comprises a substrate, a working electrode formed on the substrate, a first reference electrode formed on the substrate, a second reference electrode formed on the substrate, and a packaging structure which separates the above-mentioned three electrodes. The working electrode comprises creatinine iminohydrolase (CIH). The detection signal is transmitted out from the biosensor for further processing through a wire or an exposed surface. The disclosed biosensor is replaceable.
摘要翻译：本发明公开了一种用于检测肌酐的电位生物传感器及其形成方法。所公开的生物传感器包括基板，形成在基板上的工作电极，形成在基板上的第一参考电极，形成在基板上的第二参考电极，以及分离上述三个电极的封装结构。工作电极包括肌酸酐亚氨基水解酶（CIH）。检测信号从生物传感器传出，用于通过线或暴露表面进一步处理。所公开的生物传感器是可更换的。

15. 发明申请

US20090021263A1 Multi-Ion Potential Sensor and Fabrication thereof 审中-公开
标题翻译：多离子电势传感器及其制作
公开(公告)号：US20090021263A1
公开(公告)日：2009-01-22
申请号：US11778677
申请日：2007-07-17
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Shien-I Wei
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Shien-I Wei
IPC分类号： G01N27/00 , B05D5/12
CPC分类号： G01N27/3271
摘要： A multi-ion potential sensor is disclosed. The multi-ion potential sensor comprises a substrate, a conductive layer, an isolation layer, a tin oxide (SnO2) layer and a selective layer. The conductive layer comprises a plurality of independent conductive areas, wherein every conductive area comprises a readout area, a transmissive area and a sensing area, and the transmissive area of every conductive area is packaged by the isolation layer. The tin oxide layer comprises a plurality of independent tin oxide areas, wherein every tin oxide area is deposited on the sensing area, and the selective layer comprises a plurality of independent selective areas, wherein every selective area is set on the tin oxide area. The multi-ion potential sensor has various advantages, such as good sensitivity, low cost, simplicity, disposable, portable and data acquisition by a computer for different applications.
摘要翻译：公开了一种多离子电位传感器。多离子电位传感器包括衬底，导电层，隔离层，氧化锡（SnO 2）层和选择层。导电层包括多个独立的导电区域，其中每个导电区域包括读出区域，透射区域和感测区域，并且每个导电区域的透射区域被隔离层封装。锡氧化物层包括多个独立的氧化锡区域，其中每个氧化锡区域沉积在感测区域上，并且选择层包括多个独立的选择区域，其中每个选择区域设置在氧化锡区域上。多离子电位传感器具有各种优点，例如灵敏度高，成本低，简便，一次性，便携式和数据采集的计算机不同应用。

16. 发明申请

US20090020421A1 Voltage-Type Gas Concentration Sensor and Sensing Method 审中-公开
标题翻译：电压式气体浓度传感器和传感方法
公开(公告)号：US20090020421A1
公开(公告)日：2009-01-22
申请号：US11778673
申请日：2007-07-17
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Szu-Ping Chen , June-Jie Feng
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Szu-Ping Chen , June-Jie Feng
IPC分类号： G01N27/407
CPC分类号： G01N27/3335 , G01N27/406
摘要： This invention discloses a voltage-type gas concentration sensor and sensing method thereof. An ionic sensing electrode, a solid electrolyte membrane and a gas-permeable membrane are provided. The ionic sensing electrode comprises a sensing window. The solid electrolyte membrane is disposed on the sensing window. The gas-permeable membrane is disposed on the solid electrolyte membrane. By detecting the change of a reaction voltage as a result of reaction between the ionic sensing electrode and a solution to be measured, the invention is able to determine the pH of the solution to be measured.
摘要翻译：本发明公开了一种电压型气体浓度传感器及其感测方法。提供离子感测电极，固体电解质膜和透气膜。离子感测电极包括感测窗口。固体电解质膜设置在感测窗上。透气膜设置在固体电解质膜上。通过检测作为离子感测电极和待测溶液之间的反应的结果的反应电压的变化，本发明能够测定待测溶液的pH。

17. 发明授权

US07758733B2 Dual type potentiometric creatinine biosensor 失效
标题翻译：双电位电位生物传感器
公开(公告)号：US07758733B2
公开(公告)日：2010-07-20
申请号：US11457589
申请日：2006-07-14
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-Wei Pan , Nien-Hsuan Chou
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Chung-Wei Pan , Nien-Hsuan Chou
IPC分类号： G01N27/327 , G01N27/333
CPC分类号： C12Q1/002 , C12Q1/005 , G01N33/70
摘要： In this invention, a separative structure of the ion-selecting electrode is applied to fabricate the dual type potentiometric biosensor for creatinine detection. According to the fabrication process, the creatinine enzyme is immobilized onto a conductive layer of a substrate of pH sensing membrane and ammonium ion-selecting membrane. The conductive layer provides with a sensing region and a non-sensing region. A conductive line is extended from the conductive layer for using as an external electrical contact point. The dual type potentiometric creatinine biosensor was fabricated by using the enzyme immobilization method onto the surface of the selecting membrane of sensing membrane of pH sensing electrode and creatinine sensor.
摘要翻译：在本发明中，应用离子选择电极的分离结构来制造用于肌酸酐检测的双重电位生物传感器。根据制造方法，将肌酸酐酶固定在pH感应膜和铵离子选择膜的底物的导电层上。导电层提供感测区域和非感测区域。导电线从导电层延伸以用作外部电接触点。通过使用酶固定法在pH感应电极和肌酐传感器的感应膜选择膜的表面上制作双电位电位生物传感器。

18. 发明申请

US20090294284A1 REFERENCE ELECTRODE 审中-公开
标题翻译：参考电极
公开(公告)号：US20090294284A1
公开(公告)日：2009-12-03
申请号：US12128063
申请日：2008-05-28
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Tza-Wei Huang
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Tza-Wei Huang
IPC分类号： C25B11/00
CPC分类号： G01N27/301
摘要： A reference electrode is disclosed. The reference electrode is developed by spreading an aqueous KCl gelling agar solid gel/poly(vinyl chloride)carboxylated (PVC—COOH) double layer on a screen-printing Ag/AgCl electrode body. Such polymer double layer can maintain ion concentration and keep stable chemical capacitance potential during measurement. The reference electrode of the present invention provides fast response time and high stability for major ions detection measurements in a wide range.
摘要翻译：公开了参考电极。通过在丝网印刷Ag / AgCl电极体上涂布KCl胶凝琼脂固体凝胶/聚（氯乙烯）羧化（PVC-COOH）双层水溶液来显影参比电极。这样的聚合物双层可以保持离子浓度并在测量期间保持稳定的化学电容电位。本发明的参比电极为大范围的主要离子检测测量提供了快速响应时间和高稳定性。

19. 发明申请

US20090022629A1 SOLID-STATE UREA BIOSENSOR 审中-公开
标题翻译：固态尿素生物传感器
公开(公告)号：US20090022629A1
公开(公告)日：2009-01-22
申请号：US11778674
申请日：2007-07-17
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Chung-We Pan
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Chung-We Pan
IPC分类号： G01N27/333 , B01J19/00
CPC分类号： G01N33/493 , G01N33/54373 , G01N33/84
摘要： A solid-state urea biosensor may comprise a substrate, an electrically conductive layer, a PH sensing film, an ammonium ion selecting film and a ferment film. The electrically conductive layer covers the substrate. The PH sensing film has a PH value measuring zone, partially covering the electrically conductive layer, for measuring the PH value of a solution to be tested. The ammonium ion selecting film has an ammonium ion measuring zone for measuring the ammonium ion concentration. The ammonium ion selecting film partially covers the PH sensing film and exposes the PH value measuring zone. The ferment film is used for measuring the urea concentration in the solution, wherein the ferment film partially covers the ammonium ion selecting film and exposes the ammonium ion measuring zone.
摘要翻译：固态尿素生物传感器可以包括基底，导电层，PH感测膜，铵离子选择膜和发酵膜。导电层覆盖基板。 PH感测膜具有部分覆盖导电层的PH值测量区，用于测量待测溶液的PH值。铵离子选择膜具有用于测量铵离子浓度的铵离子测量区。铵离子选择膜部分地覆盖PH感测膜并暴露PH值测量区。发酵膜用于测量溶液中的尿素浓度，其中发酵膜部分地覆盖铵离子选择膜并暴露铵离子测量区。

20. 发明申请

US20090020423A1 Potentiometric Mg2+ Sensor and Method thereof 审中-公开
标题翻译：电位式Mg2 +传感器及其方法
公开(公告)号：US20090020423A1
公开(公告)日：2009-01-22
申请号：US11778676
申请日：2007-07-17
申请人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Wei-Feng Liang
发明人： Shen-Kan Hsiung , Jung-Chuan Chou , Tai-Ping Sun , Nien-Hsuan Chou , Wei-Feng Liang
IPC分类号： G01N27/414 , C23C14/34
CPC分类号： G01N27/333
摘要： A potentiometric Mg2+ sensor is disclosed, wherein the potentiometric SnO2/ITO-based Mg2+ ISE was developed in this invention. The magnesium ion-selective membrane was fabricated and dripped on the surface of SnO2. The performance, such as sensitivity, was exhibited by the magnesium ion-selective membrane having magnesium ionophore, K-TpClPB, plasticizer, PVC in the suitable ratios. Moreover, the Mg2+ ISE was measured in different Mg2+ concentration buffer solutions. According to the experimental results, the best sensitivity of the Mg2+ sensor is 31.7l mV/decade between 10-4M and 10-1M, and measurement time is 30 sec.
摘要翻译：公开了一种电位计Mg2 +传感器，其中在本发明中开发了基于电位的SnO 2 / ITO基的Mg 2+ + ISE。制备镁离子选择膜并滴在SnO2表面。具有镁离子载体的镁离子选择膜，K-TpClPB，增塑剂，PVC以适当的比例表现出诸如灵敏度的性能。此外，在不同的Mg2 +浓度缓冲溶液中测量Mg2 + ISE。根据实验结果，在10-4M和10-1M之间，Mg2 +传感器的最佳灵敏度为31.7lm /次，测量时间为30秒。

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