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11. 发明申请

US20050053939A1 Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents 审中-公开
标题翻译：用于增强使用可切割电泳标签试剂的测定中检测的方法和组合物
公开(公告)号：US20050053939A1
公开(公告)日：2005-03-10
申请号：US10494879
申请日：2002-11-08
申请人： Ahmed Chenna , Sharat Singh
发明人： Ahmed Chenna , Sharat Singh
IPC分类号： G01N33/532 , C12Q1/68 , G01N33/53
CPC分类号： G01N33/532
摘要： Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region, both linked to a target-binding moiety. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Also described are biopolymers and nucleosides containing such interactive functionalities.
摘要翻译：提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。检测涉及到识别标签作为目标识别的结果。探针组包括电泳标签探针或e-标签探针，其包含与靶结合部分连接的检测区域和迁移率定义区域。在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。这些探针包括与位于探针中的切割部分相邻的交互功能，使得交互功能不构成电子标签记录器的一部分。还描述了含有这种相互作用功能的生物聚合物和核苷。

12. 发明授权

US07771929B2 Tag library compounds, compositions, kits and methods of use 有权
标题翻译：标签库化合物，组合物，试剂盒和使用方法
公开(公告)号：US07771929B2
公开(公告)日：2010-08-10
申请号：US10420549
申请日：2003-04-18
申请人： Sharat Singh , Tracy Matray , Hossein Salimi-Moosavi
发明人： Sharat Singh , Tracy Matray , Hossein Salimi-Moosavi
IPC分类号： C07B61/00 , C07H21/00
CPC分类号： C40B70/00 , C07H21/00 , C40B50/16
摘要： Families of compositions are provided as labels, referred to as eTag reporters for attaching to polymeric compounds and assaying based on release of the eTag reporters from the polymeric compound and separation and detection. For oligonucleotides, the eTag reporters are synthesized at the end of the oligonucleotide by using phosphite or phosphate chemistry, whereby mass-modifying regions, charge-modifying regions and detectable regions are added sequentially to produce the eTag labeled reporters. By using small building blocks and varying their combination large numbers of different eTag reporters can be readily produced attached to a binding compound specific for the target compound of interest for identification. Protocols are used that release the eTag reporter when the target compound is present in the sample.
摘要翻译：提供组合物家族作为标记，被称为eTag报告基因，用于附着于聚合物上，并基于从高分子化合物释放eTag报告物并进行分离和检测。对于寡核苷酸，通过使用亚磷酸酯或磷酸酯化学在寡核苷酸的末端合成eTag报告物，由此顺序加入质量修饰区域，电荷修饰区域和可检测区域以产生eTag标记的记录。通过使用小的构建块并改变它们的组合，可以容易地产生大量不同的eTag报告物，其连接到目的化合物特异性的结合化合物用于鉴定。当目标化合物存在于样品中时，使用释放eTag报告物的方案。

13. 发明申请

US20060223107A1 Detection of nucleic acid sequences by cleavage and separation of tag-containing structures 审中-公开
标题翻译：通过裂解和分离含标记结构来检测核酸序列
公开(公告)号：US20060223107A1
公开(公告)日：2006-10-05
申请号：US11447753
申请日：2006-06-05
申请人： Ahmed Chenna , Vivian Xiao , Sharat Singh
发明人： Ahmed Chenna , Vivian Xiao , Sharat Singh
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6816 , C07H19/06 , C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/682 , C12Q1/6823 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , C12Q2565/125 , C12Q2537/162 , C12Q2521/301 , C12Q2537/125 , C12Q2525/161
摘要： The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.
摘要翻译：本发明涉及通过用一对探针形成和切割双链体结构来检测多个靶核酸序列的方法，每对中的一个探针用电泳标签标记。双链结构的切割释放电泳标签，然后将其分离并鉴定以指示靶序列的存在或数量。本发明特别适用于在一个反应中检测多个靶序列的多重反应。还提供了用于检测核酸的试剂盒。

14. 发明授权

US06649351B2 Methods for detecting a plurality of analytes by mass spectrometry 有权
公开(公告)号：US06649351B2
公开(公告)日：2003-11-18
申请号：US10008593
申请日：2001-11-09
申请人： Tracy J. Matray , Vincent S. Hernandez , Ahmed Chenna , Herbert Hooper , Sharat Singh
发明人： Tracy J. Matray , Vincent S. Hernandez , Ahmed Chenna , Herbert Hooper , Sharat Singh
IPC分类号： C12Q168
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/6816 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , G01N33/561 , C12Q2521/319 , C12Q2563/131 , C12Q2565/627
摘要： The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first binding reagents containing a cleavage-inducing moiety and a target binding moiety, each of the second binding reagents containing a tagged probe having a mass modifier region attached to a target binding moiety by a cleavable linkage, the cleavable linkage being susceptible to cleavage when in proximity to an activated cleavage-inducing moiety; (b) activating the cleavage-inducing moiety to release a tag reporter, and (c) detecting a mass of the tag reporter, the mass uniquely corresponding to a known target analyte.

15. 发明授权

US09110075B2 Compositions for analyzing proteins 有权
标题翻译：用于分析蛋白质的组合物
公开(公告)号：US09110075B2
公开(公告)日：2015-08-18
申请号：US13252778
申请日：2011-10-04
申请人： Sharat Singh , Hossein Salimi-Moosavi , Syed Hasan Tahir , Gerald J. Wallweber , Hrair Kirakossian , Tracy J. Matray , Vincent S. Hernandez
发明人： Sharat Singh , Hossein Salimi-Moosavi , Syed Hasan Tahir , Gerald J. Wallweber , Hrair Kirakossian , Tracy J. Matray , Vincent S. Hernandez
IPC分类号： G01N33/533 , C07K1/13 , G01N33/68
CPC分类号： G01N33/6842 , C07K16/00 , C07K2317/40 , G01N33/582 , G01N33/6803
摘要： Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification.
摘要翻译：公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。

16. 发明授权

US08574855B2 Assays for the detection of anti-TNF drugs and autoantibodies 有权
标题翻译：用于检测抗TNF药物和自身抗体的测定
公开(公告)号：US08574855B2
公开(公告)日：2013-11-05
申请号：US13441727
申请日：2012-04-06
申请人： Sharat Singh , Shui Long Wang , Linda Ohrmund
发明人： Sharat Singh , Shui Long Wang , Linda Ohrmund
IPC分类号： G01N33/53
CPC分类号： G01N33/564 , G01N33/5091 , G01N33/6854 , G01N2800/52
摘要： The present invention provides assays for detecting and measuring the presence or level of anti-TNFα drug therapeutics and autoantibodies in a sample. The present invention is useful for optimizing therapy and monitoring patients receiving anti-TNFα drug therapeutics to detect the presence or level of autoantibodies (e.g., HACA and/or HAHA) against the drug.
摘要翻译：本发明提供用于检测和测量样品中抗TNFα药物治疗剂和自身抗体的存在或水平的测定。本发明可用于优化治疗和监测接受抗TNFα药物治疗剂的患者，以检测针对药物的自身抗体（例如，HACA和/或HAHA）的存在或水平。

17. 发明授权

US08198031B2 Detecting and profiling molecular complexes 有权
标题翻译：检测和分析分子复合物
公开(公告)号：US08198031B2
公开(公告)日：2012-06-12
申请号：US11717760
申请日：2007-03-12
申请人： Po-Ying Chan-Yui , Sharat Singh , Hossein Salimi-Moosavi , Yining Shi , Sailaja Pidaparthi , Rajiv Dua , Ali Mukherjee
发明人： Po-Ying Chan-Yui , Sharat Singh , Hossein Salimi-Moosavi , Yining Shi , Sailaja Pidaparthi , Rajiv Dua , Ali Mukherjee
IPC分类号： G01N33/53 , G01N33/532 , G01N33/554 , C07K1/10 , C07K1/14
CPC分类号： G01N33/566 , G01N33/542 , Y10S436/824
摘要： Methods are provided for detecting the formation of complexes of molecules, especially proteins, in a sample, such as a cell or tissue lysate. In one aspect, a cleaving probe specific for a first protein in a complex and one or more binding compounds specific for one or more second proteins in a complex are provided. Upon binding, the cleaving probe is induced to generate an active species, such as singlet oxygen, that cleaves molecular tags attached to the binding compounds only in the local region of the cleaving probe. The released molecular tags are separated from the assay mixture and from one another to provide a readout that is related to the number and types of proteins present in the complex.
摘要翻译：提供了用于检测样品（例如细胞或组织裂解物）中分子复合物（特别是蛋白质）的形成的方法。在一个方面，提供了对复合物中的第一种蛋白质特异性的切割探针和一种或多种特异于复合物中的一种或多种第二种蛋白质的结合化合物。在结合时，诱导切割探针以产生仅在切割探针的局部区域中切割附着于结合化合物的分子标签的活性物质，例如单线态氧。将释放的分子标签与测定混合物和彼此分离，以提供与复合物中存在的蛋白质的数量和类型相关的读数。

18. 发明申请

US20110159521A1 METHODS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME 有权
标题翻译：用于诊断可刺激性牙刷综合征的方法
公开(公告)号：US20110159521A1
公开(公告)日：2011-06-30
申请号：US12862707
申请日：2010-08-24
申请人： Hua Gong , Shui Long Wang , Sharat Singh
发明人： Hua Gong , Shui Long Wang , Sharat Singh
IPC分类号： G01N33/573 , G01N33/53 , G01N33/92
CPC分类号： G01N33/573 , G01N33/6893 , G01N33/74 , G01N2800/065 , G01N2800/56 , Y10S436/809 , Y10T436/147777
摘要： The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E2.
摘要翻译：本发明提供了使用兔抗类胰蛋白酶作为捕获抗体和碱性磷酸酶缀合的G3作为检测抗体的ELISA测定法来测定血清肥大细胞和bgr-酪氨酸酶水平。使用发光底物CPSD增强测定灵敏度。还提供了通过检测血清中β-谷氨酸酶，组胺和/或前列腺素E2的水平来帮助诊断肠易激综合征的方法。

19. 发明申请

US20110045476A1 INFLAMMATORY BOWEL DISEASE PROGNOSTICS 有权
标题翻译：炎症性皮肤疾病预防
公开(公告)号：US20110045476A1
公开(公告)日：2011-02-24
申请号：US12851517
申请日：2010-08-05
申请人： Derren Barken , Fred Princen , Leonard Eggleston , Sharat Singh
发明人： Derren Barken , Fred Princen , Leonard Eggleston , Sharat Singh
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6883 , C12Q2600/106 , C12Q2600/112 , C12Q2600/118 , C12Q2600/156 , G01N33/564 , G01N33/6893 , G01N2800/065 , G01N2800/56 , Y10T436/143333
摘要： The methods and systems of the present invention are useful in the diagnosis of inflammatory bowel disease (IBD) and in the prognosis of IBD progression and disease complications. With the present invention, it is possible to predict outcome of disease and patients who will have a particular risk of disease complications and/or progression to surgery.
摘要翻译：本发明的方法和系统可用于诊断炎症性肠病（IBD）和IBD进展和疾病并发症的预后。利用本发明，可以预测疾病的结果和将具有疾病并发症和/或进展到手术的特定风险的患者。

20. 发明申请

US20080261829A1 ANTIBODY-BASED ARRAYS FOR DETECTING MULTIPLE SIGNAL TRANSDUCERS IN RARE CIRCULATING CELLS 有权
标题翻译：用于检测罕见循环细胞中多个信号转导体的基于抗体的阵列
公开(公告)号：US20080261829A1
公开(公告)日：2008-10-23
申请号：US12046381
申请日：2008-03-11
申请人： Jeanne Harvey , Sharat Singh , Phillip Kim , Xinjun Liu , Robert Barham , Limin Liu
发明人： Jeanne Harvey , Sharat Singh , Phillip Kim , Xinjun Liu , Robert Barham , Limin Liu
IPC分类号： C40B40/00 , G01N33/53
CPC分类号： G01N33/574 , C40B30/08 , C40B40/10 , G01N33/542 , G01N33/57415 , G01N33/582 , G01N33/6845 , G01N33/6854 , G01N2333/902 , G01N2333/908
摘要： The present invention provides antibody-based arrays for detecting the activation state and/or total amount of a plurality of signal transduction molecules in rare circulating cells and methods of use thereof for facilitating cancer prognosis and diagnosis and the design of personalized, targeted therapies.
摘要翻译：本发明提供用于检测稀有循环细胞中多个信号转导分子的活化状态和/或总量的基于抗体的阵列及其用于促进癌症预后和诊断以及个性化靶向治疗的设计的方法。

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