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11. 发明授权

US09096872B2 Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same 有权
标题翻译：具有提高丁醇生产能力的重组微生物和使用其生产丁醇的方法
公开(公告)号：US09096872B2
公开(公告)日：2015-08-04
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N9/04 , C12N9/10
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

12. 发明申请

US20100136640A1 ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME 审中-公开
标题翻译：生产微生物的增强的丁醇和使用它们制备丁醇的方法
公开(公告)号：US20100136640A1
公开(公告)日：2010-06-03
申请号：US12519060
申请日：2007-12-14
申请人： Sang Yup Lee , Jin Hwan Park , Eleftherios Terry Papoutsakis
发明人： Sang Yup Lee , Jin Hwan Park , Eleftherios Terry Papoutsakis
IPC分类号： C12P7/16 , C12N1/21 , C12N1/19 , C12N1/15
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified.
摘要翻译：本发明涉及具有提高的丁醇生产能力的重组突变体微生物和使用其的丁醇生产方法。在微生物中，编码负责乳酸盐，乙醇和/或乙酸盐的生物合成的酶的基因被缺失或减弱，并且引入并扩增了参与丁醇生物合成的酶编码基因。

13. 发明授权

US07752540B2 Information system for metabolic flux analysis using extensible markup language and operating method thereof 失效
标题翻译：使用可扩展标记语言进行代谢通量分析的信息系统及其操作方法
公开(公告)号：US07752540B2
公开(公告)日：2010-07-06
申请号：US11148907
申请日：2005-06-09
申请人： Sang Yup Lee , Hong Seok Yun , Dong Yup Lee , Seung Hyun Lee , Joon Woo Jeong , Tae Yong Kim
发明人： Sang Yup Lee , Hong Seok Yun , Dong Yup Lee , Seung Hyun Lee , Joon Woo Jeong , Tae Yong Kim
IPC分类号： G06F17/00
CPC分类号： G06F19/26 , G06F19/12
摘要： The present invention relates to an MFA (Metabolic Flux Analysis) information system using an XML (eXtensible Markup Language) and an operating method thereof. More specifically, the invention relates to an MFA information system and an operating method thereof, which generates, edits, stores and visualizes an MFA model feature and an MFA object using XML, and edits, stores and visualizes the result obtained by performing MFA based on the object. The present invention provides the MFA information system and method capable of generating, editing, storing and visualizing MFA model features and MFA objects using XML. Accordingly, MFA can be easily performed by utilizing advantages of XML, such as transplantation, reusability, deciphering, scalability, flexibility and effective data exchange, and thus the present invention can be applied to cell improvement using metabolic engineering.
摘要翻译：本发明涉及使用XML（可扩展标记语言）的MFA（代谢通量分析）信息系统及其操作方法。更具体地，本发明涉及一种MFA信息系统及其操作方法，其使用XML生成，编辑，存储和可视化MFA模型特征和MFA对象，并且通过执行MFA获得的结果进行编辑，存储和可视化物体。本发明提供了能够使用XML来生成，编辑，存储和可视化MFA模型特征和MFA对象的MFA信息系统和方法。因此，通过利用移植，可重用性，解密，可扩展性，灵活性和有效的数据交换等XML的优点，可以容易地实现MFA，因此本发明可以应用于使用代谢工程的细胞改良。

14. 发明申请

US20090215048A1 METHOD OF IN-SILICO IMPROVEMENT OF ORGANISMS USING THE FLUX SUM OF METABOLITES 审中-公开
标题翻译：使用代谢物通量的有机硅改善方法
公开(公告)号：US20090215048A1
公开(公告)日：2009-08-27
申请号：US11994330
申请日：2005-10-14
申请人： Sang Yup Lee , Tae Yong Kim , Dong Yup Lee
发明人： Sang Yup Lee , Tae Yong Kim , Dong Yup Lee
IPC分类号： C12Q1/68 , G06F19/00 , C12N15/00 , C12N15/74 , C12P7/46
CPC分类号： G16B5/00 , G16B20/00
摘要： The present invention relates to an in silico method for improving an organism on the basis of the flux sum (φ) of metabolites, and more particularly to a method for screening key metabolites that increase the production yield of a useful substance, the method comprising defining the metabolite utilization of an organism for producing a useful substance as flux sum and perturbing the flux sum, as well as a method for improving an organism producing a useful substance, the method comprising deleting and/or amplifying genes associated with the aforementioned screened key metabolites. According to the present invention, the correlation between specific metabolites and useful substance production can be exactly predicted, so that it is possible to develop an organism having increased useful substance production by introducing and/or amplifying and/or deleting genes expressing enzymes associated with the specific metabolites. In addition, it is also possible to increase the production of a useful substance by adding specific metabolites during culture.
摘要翻译：本发明涉及一种基于代谢物的通量和（phi）改善生物体的计算机方法，更具体地涉及一种筛选提高有用物质产量的关键代谢物的方法，该方法包括定义用于产生有用物质的生物体的代谢物利用作为助熔剂和扰乱通量和，以及改善产生有用物质的生物体的方法，所述方法包括删除和/或扩增与上述筛选的关键代谢物相关的基因。根据本发明，可以准确地预测特定代谢物和有用物质生产之间的相关性，从而可以通过引入和/或扩增和/或删除表达与...相关的酶的基因来开发具有增加的有用物质生产的生物体特异性代谢物。此外，还可以通过在培养期间加入特定的代谢物来增加有用物质的产生。

15. 发明申请

US20090075352A1 Method For Improving A Strain Based On In-Silico Analysis 审中-公开
标题翻译：基于硅胶分析改进应变的方法
公开(公告)号：US20090075352A1
公开(公告)日：2009-03-19
申请号：US11722632
申请日：2005-05-23
申请人： Sang Yup Lee , Tae Yong Kim , Dong Yup Lee , Sang Jun Lee
发明人： Sang Yup Lee , Tae Yong Kim , Dong Yup Lee , Sang Jun Lee
IPC分类号： C12P7/46 , G06G7/48 , C12N1/21
CPC分类号： C12P7/46 , C12N9/1205 , C12N15/1089
摘要： The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.
摘要翻译：本发明涉及一种基于在计算机分析中改进菌株的方法，其中将用于产生有用物质的靶菌株的基因组信息与过量产生有用物质的菌株的基因组信息进行比较，以便主要是用于过量生产有用物质的筛选基因，然后通过用代谢通量分析进行模拟来次要筛选待缺失的基因。根据本发明，可以通过代谢和基因工程方法有效地构建改进的菌株，其包括比较分析用于产生有用物质的靶菌株的基因组信息和产生大量有用物质的菌株的基因组信息筛选候选基因并在筛选的候选基因的计算机模拟中进行选择要缺失的基因的组合，其显示有用物质的生产的改进。因此，可以显着降低实际湿测试所需的时间，精力和成本。

16. 发明授权

US07803587B2 Method for developing culture medium using genome information and in silico analysis 有权
标题翻译：使用基因组信息和计算机分析开发培养基的方法
公开(公告)号：US07803587B2
公开(公告)日：2010-09-28
申请号：US11743668
申请日：2007-05-02
申请人： Sang Yup Lee , Ho Nam Chang , Hyohak Song , Tae Yong Kim , Bo-Kyung Choi
发明人： Sang Yup Lee , Ho Nam Chang , Hyohak Song , Tae Yong Kim , Bo-Kyung Choi
IPC分类号： C12P7/44 , C12N1/20 , C12Q1/00 , C12Q1/68 , G06G7/48
CPC分类号： C12N1/20 , C12N5/0018 , C12N2500/32 , C12N2500/38 , C12N2500/40 , C12P7/46 , G06F19/12
摘要： A method for developing a culture medium using genome information and in silico analysis. In one implementation, the method involves developing a minimal synthetic medium, including the steps of constructing a metabolic network using genome information of prokaryotic cell or eukaryotic cell, selecting components of the minimal synthetic medium removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation, and determining a final culture medium by actual culture.
摘要翻译：使用基因组信息和计算机分析开发培养基的方法。在一个实施方案中，该方法包括开发最小的合成培养基，包括使用原核细胞或真核细胞的基因组信息构建代谢网络的步骤，从构建的代谢网络中选择去除外部代谢物中的任何一种的最小合成培养基的组分，以及使用计算机模拟进行代谢通量分析，并通过实际培养确定最终培养基。

17. 发明授权

US09145583B2 Multi-spot metal-capped nanostructure array nucleic acid chip for diagnosis of corneal dystrophy and preparation method thereof 有权
标题翻译：用于诊断角膜营养不良的多点金属封端纳米结构阵列核酸芯片及其制备方法
公开(公告)号：US09145583B2
公开(公告)日：2015-09-29
申请号：US13391167
申请日：2010-08-18
申请人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
发明人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
IPC分类号： C12Q1/68 , C12M1/36 , B82Y30/00
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.
摘要翻译：一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

18. 发明授权

US08895276B2 Microorganism variants having hydrocarbon producing ability and method for producing hydrocarbon using the same 有权
标题翻译：具有烃生产能力的微生物变体及使用其生产烃的方法
公开(公告)号：US08895276B2
公开(公告)日：2014-11-25
申请号：US13702920
申请日：2011-06-10
申请人： Sang Yup Lee , Yong Jun Choi
发明人： Sang Yup Lee , Yong Jun Choi
IPC分类号： C12P5/00 , C12N9/02 , C12N9/18 , C12N9/88 , C12N9/00 , C12P5/02 , C12N15/52 , C12N15/70 , C07K14/245
CPC分类号： C12P5/00 , C07K14/245 , C12N9/001 , C12N9/18 , C12N9/88 , C12N9/93 , C12N15/52 , C12N15/70 , C12P5/005 , C12P5/02 , C12P5/026
摘要： The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.
摘要翻译：本发明涉及具有生产碳氢化合物（包括烷烃）的能力的微生物变异体，以及使用该方法生产烃类的方法，包括使用烷烃的烷烃，更具体地说，涉及通过引入编码转化脂肪酶的基因而获得的微生物变体对游离脂肪酸的酰基-Acp，编码将游离脂肪酸转化为脂肪酰辅酶A的酶的基因，编码将脂肪酰基-CoA转化为脂肪醛的酶的基因和编码将脂肪醛转化为烷烃的酶的基因转化为微生物改进以适于生产包括烷烃在内的烃类，以及使用其制备包括烷烃在内的烃类的方法。本发明的微生物变体具有很高的潜力用于通过附加的代谢通量工程来改善菌株，因此可用于包括烷烃在内的烃的工业生产。

19. 发明授权

US08877482B2 Homo-succinic acid producing microorganism variant and process for preparing succinic acid using the same 有权
标题翻译：产琥珀酸的微生物变异体及使用其制备琥珀酸的方法
公开(公告)号：US08877482B2
公开(公告)日：2014-11-04
申请号：US12668868
申请日：2008-01-15
申请人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
发明人： Sang Yup Lee , Ji Mahn Kim , Jeong Wook Lee , Hyohak Song , Sol Choi
IPC分类号： C12N1/20 , C12N15/00 , C12N15/74 , C12P7/46 , C12N9/04 , C12N9/12
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏乳酸脱氢酶编码基因（idhA）和一种乙酸激酶编码基因（ackA），以及一种高浓度均聚琥珀酸的制备方法，其包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

20. 发明申请

US20140243222A1 MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSING OF CORNEAL DYSTROPHY AND PREPARATION METHOD THEREOF PRODUCING SAME 有权
标题翻译：多点金属填充纳米结构阵列核苷酸芯片，用于诊断角膜晶状体及其制备方法
公开(公告)号：US20140243222A1
公开(公告)日：2014-08-28
申请号：US13391167
申请日：2010-08-18
申请人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
发明人： Sang Yup Lee , So Young Yoo , Do Kyun Kim , Tae Jung Park , Jung Kuk Yun , Gene Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00509 , B01J2219/00608 , B01J2219/00612 , B01J2219/00614 , B01J2219/00621 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B82Y30/00 , C12Q1/6883 , C12Q2600/158
摘要： A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy, and more particularly to a multi-spot metal-capped nanostructure array nucleic acid chip capable of employing LSPR (localized surface plasmon resonance) optical properties, a preparation method thereof, and a multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations, which can diagnose various corneal dystrophies. The metal-capped nanostructure array nucleic acid chip can be combined with analysis devices, including a light source, a detector, a spectrophotometer and a computer, to provide an LSPR optical property-based optical biosensor, and the use of the multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing BIGH3 gene mutations allows the simultaneous diagnosis of various corneal dystrophies that are genetic ocular diseases.
摘要翻译：一种用于诊断角膜营养不良的多点金属帽纳米结构阵列核酸芯片，更具体地涉及能够使用LSPR（局部表面等离子共振）光学性质的多点金属封端的纳米结构阵列核酸芯片，其制备方法，以及用于诊断BIGH3基因突变的多点金属帽纳米结构阵列核酸芯片，其可以诊断各种角膜营养不良。金属封端的纳米结构阵列核酸芯片可以与包括光源，检测器，分光光度计和计算机的分析装置组合，以提供基于LSPR光学性质的光学生物传感器，并且使用多点金属用于诊断BIGH3基因突变的封闭的纳米结构阵列核酸芯片允许同时诊断作为遗传性眼部疾病的各种角膜营养不良。

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