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    • 12. 发明授权
    • Method of identifying maize plants having increased oil content using quantitative trait locus 6 (QTL6)
    • 使用数量性状位点6(QTL6)鉴定具有增加的油含量的玉米植物的方法
    • US08084208B2
    • 2011-12-27
    • US12875574
    • 2010-09-03
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • C12Q1/68A01H1/00A01H5/00
    • C12N15/8247C12N9/1029C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
    • Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
    • 提供了与玉米数量性状基因座6(QTL6)相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座,其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中,新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的用于操纵植物或植物部分的油和/或油酸含量和/或油酸/亚油酸比例的方法,用于标记辅助选择 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物,例如玉米植物或其植物部分,以及高油的标记辅助育种 和/或高油酸性状。
    • 13. 发明申请
    • COMPOSITIONS RELATED TO THE QUANTITATIVE TRAIT LOCUS 6 (QTL6) IN MAIZE AND METHODS OF USE
    • 与量化条件6(QTL6)相关的组合物及其使用方法
    • US20110252499A1
    • 2011-10-13
    • US13157068
    • 2011-06-09
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • A01H1/02A01H5/00A01H5/10C12Q1/68
    • A01H1/04C12N9/1029C12N15/8247C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172Y10T436/104165
    • Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
    • 提供了与玉米数量性状基因座6(QTL6)相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座,其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中,新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物,例如玉米植物或其植物部分,以及高油的标记辅助育种 和/或高油酸性状。
    • 16. 发明授权
    • Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use
    • 与玉米的数量性状基因座6(QTL6)和使用方法有关的成分
    • US07812216B2
    • 2010-10-12
    • US11680922
    • 2007-03-01
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • William B. AllenBo ShenMitchell C. TarczynskiMark E. WilliamsPeizhong ZhengGan-Yuan Zhong
    • A01H1/00A01H5/00A01H5/10C12N15/82
    • C12N15/8247C12N9/1029C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
    • Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
    • 提供了与玉米数量性状基因座6(QTL6)相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座,其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中,新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中 具有增加的油含量和/或增加的油酸含量和/或油酸/亚油酸比例增加的植物,例如玉米植物或其植物部分,以及用于高油的标记辅助育种 和/或高油酸性状。
    • 19. 发明申请
    • ODP2 promoter and methods of use
    • ODP2启动子和使用方法
    • US20050223432A1
    • 2005-10-06
    • US11000752
    • 2004-12-01
    • Shane AbbittWilliam Gordon-KammKeith LowePeizhong Zheng
    • Shane AbbittWilliam Gordon-KammKeith LowePeizhong Zheng
    • A01H1/00A01H5/00C07K14/415C12N5/10C12N15/29C12N15/82
    • C12N15/8234C07K14/415
    • Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for promoter sequences isolated from the maize Ovule Development Protein 2 (ODP2) gene. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is further provided. The method comprises introducing into a plant or plant cell an expression cassette comprising an ODP2 promoter of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in regulating expression of nucleotide sequences of interest in a seed-preferred manner. Transformed plants, plant cells, and seeds comprising the ODP2 promoter sequence or variants and fragments thereof are also provided.
    • 提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物包括用于从玉米Ovule发育蛋白2(ODP2)基因分离的启动子序列的新型核酸分子及其变体和片段。 还提供使用本文公开的启动子序列在植物中表达目的核苷酸序列的方法。 该方法包括向植物或植物细胞中引入包含可操作地连接到感兴趣的核苷酸序列的本发明的ODP2启动子的表达盒。 特别地,组合物和方法可用于以种子优选的方式调节感兴趣的核苷酸序列的表达。 还提供了转化的植物,植物细胞和包含ODP2启动子序列或其变体和片段的种子。