专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明授权

US09551023B2 Sample preparation method 有权
标题翻译：样品制备方法
公开(公告)号：US09551023B2
公开(公告)日：2017-01-24
申请号：US14427554
申请日：2013-09-06
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： Daniel John Turner , Clive Gavin Brown , Stuart William Reid , James Anthony Clarke , James White , David Jackson Stoddart
IPC分类号： C12P19/34 , C07H21/00 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6806 , C07H21/04 , C12Q1/6869 , C12Q2525/119 , C12Q2525/301 , C12Q2531/125 , C12Q2533/107 , C12Q2565/631
摘要： The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.
摘要翻译：本发明涉及用于修饰模板多核苷酸进行表征的方法，特别是用于纳米孔测序。该方法产生修饰的多核苷酸，其在某些位置与模板多核苷酸互补，并且在另一个位置包含通用或非碱基核苷酸，并且在某些情况下可以是可预测的位置。然后可以表征所得修饰的多核苷酸。

12. 发明申请

US20220213541A1 METHOD FOR DETERMINING A POLYMER SEQUENCE 有权
公开(公告)号：US20220213541A1
公开(公告)日：2022-07-07
申请号：US17272986
申请日：2019-09-04
申请人： Oxford Nanopore Technologies Limited
发明人： Clive Gavin Brown , Timothy Lee Massingham , Stuart William Reid
IPC分类号： C12Q1/6869 , G16B40/10 , G16B40/20
摘要： The invention resides in a method of determining a sequence of a target polymer, or part thereof, comprising polymer units comprising canonical and non-canonical polymer units. The method comprises taking a series of measurements of a signal relating to the target polymer wherein a measurement of the signal is dependent upon a plurality of polymer units, and wherein the polymer units of the target polymer modulate the signal, and wherein a non-canonical polymer unit modulates the signal differently from a corresponding canonical polymer unit. The series of measurements are analysed using a machine learning technique that attributes a measurement of a non-canonical polymer unit to being a measurement of a respective corresponding canonical polymer unit. The sequence of the target polymer, or part thereof, is determined from the analysed series of measurements. A non-canonical polymer unit identified from the analysis can be additionally or alternatively determined. Two or more types of non-canonical polymer units corresponding to the two or more types of canonical polymer unit can be used. The polynucleotide can be DNA.

13. 发明授权

US11085078B2 Method for nucleic acid detection by guiding through a nanopore 有权
公开(公告)号：US11085078B2
公开(公告)日：2021-08-10
申请号：US16338399
申请日：2017-09-29
申请人： Oxford Nanopore Technologies Limited
发明人： Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6876 , G01N33/487
摘要： The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

14. 发明申请

US20170002406A1 METHOD FOR CONTROLLING THE MOVEMENT OF A POLYNUCLEOTIDE THROUGH A TRANSMEMBRANE PORE 审中-公开
标题翻译：通过透明质子管控制多核苷酸运动的方法
公开(公告)号：US20170002406A1
公开(公告)日：2017-01-05
申请号：US15113174
申请日：2014-09-10
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： Rebecca Victoria Bowen , Clive Gavin Brown , Mark Bruce , Andrew John Heron , Elizabeth Jayne Wallace , James White , Joseph Hargreaves Lloyd , David Antoni Alves , Domenico Caprotti , Lakmal Jayasinghe , Luke McNeill , John Milton , Antonino Puglisi , Szabolcs Soeroes
IPC分类号： C12Q1/68 , G01N27/447 , G01N27/414
CPC分类号： C12Q1/6869 , G01N27/4145 , G01N27/44721 , G01N27/4473 , G01N27/44752 , G01N27/44791 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.
摘要翻译：本发明涉及通过跨膜孔控制多核苷酸运动的新方法。本发明还涉及使用解旋酶表征靶多核苷酸的新方法。

15. 发明申请

US20160162634A1 ANALYSIS OF A POLYMER COMPRISING POLYMER UNITS 审中-公开
标题翻译：包含聚合物单体的聚合物分析
公开(公告)号：US20160162634A1
公开(公告)日：2016-06-09
申请号：US14346549
申请日：2012-09-21
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： Stuart William Reid , Gavin Harper , Clive Gavin Brown , James Anthony Clarke , Andrew John Heron
IPC分类号： G06F19/22 , G01N33/483 , G06F17/18
CPC分类号： G01N33/48721 , B82Y15/00 , C12Q1/6869 , G01N27/44791 , G01N33/483 , G06F17/18 , G06N7/005 , G16B30/00 , C12Q2537/165 , C12Q2565/631
摘要： A sequence of polymer units in a polymer (3), eg. DNA, is estimated from at least one series of measurements related to the polymer, eg. ion current as a function of translocation through a nanopore (1), wherein the value of each measurement is dependent on a k-mer being a group of k polymer units (4). A probabilistic model, especially a hidden Markov model (HMM), is provided, comprising, for a set of possible k-mers: transition weightings representing the chances of transitions from origin k-mers to destination k-mers; and emission weightings in respect of each k-mer that represent the chances of observing given values of measurements for that k-mer. The series of measurements is analysed using an analytical technique, eg. Viterbi decoding, that refers to the model and estimates at least one estimated sequence of polymer units in the polymer based on the likelihood predicted by the model of the series of measurements being produced by sequences of polymer units. In a further embodiment, different voltages are applied across the nanopore during translocation in order to improve the resolution of polymer units.
摘要翻译：聚合物（3）中的聚合物单元序列，例如 DNA，从与聚合物相关的至少一系列测量估计，例如。作为通过纳米孔（1）的易位的函数的离子电流，其中每个测量的值取决于作为k个聚合物单元（4）的组的k-mer。提供概率模型，特别是隐马尔可夫模型（HMM），包括一组可能的k-mers：转换权重，表示从原始k-mers到目的地k-mers的转换机会; 和每个k-mer的发射权重，其表示观察该k-mer的给定值的测量值的机会。使用分析技术分析一系列测量结果，例如。维特比解码，其是指模型并且基于通过由聚合物单元的序列产生的一系列测量的模型预测的可能性估计聚合物中的聚合物单元的至少一个估计序列。在另一个实施方案中，在易位期间跨纳米孔施加不同的电压，以改善聚合物单元的分辨率。

16. 发明申请

US20150177237A1 APTAMER METHOD 审中-公开
标题翻译： APTAMER方法
公开(公告)号：US20150177237A1
公开(公告)日：2015-06-25
申请号：US14378929
申请日：2013-02-14
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： Daniel John Turner , Daniel George Fordham , Roger Charles Gill , Clive Gavin Brown , Stuart Reid , James Anthony Clarke , James White
IPC分类号： G01N33/543 , C12N15/115
摘要： The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.
摘要翻译：本发明涉及一种在样品中测定两种或更多种分析物组的一种或多种分析物成分的存在或不存在的新方法。因此，本发明涉及用于确定一组多种分析物中每种分析物的存在或不存在的多重测定。该测定使用适体和跨膜孔。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式