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11. 发明申请

US20070253935A1 SURFACE EXPRESSION METHOD OF PEPTIDES P5 AND ANAL3 USING THE GENE ENCODING POLY-GAMMA-GLUTAMATE SYNTHETASE 有权
标题翻译：使用基因编码多糖 - 谷氨酸合成酶的肽P5和ANAL3的表面表达方法
公开(公告)号：US20070253935A1
公开(公告)日：2007-11-01
申请号：US11622158
申请日：2007-01-11
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahim , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahim , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
IPC分类号： A61K39/00 , C12N1/21 , C12N15/00
CPC分类号： C07K14/32
摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.
摘要翻译：本发明涉及使用载体表达在微生物表面上表现出具有两亲性的肽抗生素P 5 3和Anal 3 35的各种抗细菌，抗真菌和抗癌活性61,63,65,67,69,71的方法，含有源自芽孢杆菌属的外膜蛋白基因（pgsBCA）。菌株并参与了聚-γ-谷氨酸的合成。此外，本发明涉及具有表面表达的肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。因此，本发明的方法具有非常高的工业实用性。此外，除了P 5 3和Anal 3 35之外，本发明可以应用于其他肽抗生素。

12. 发明申请

US20060199185A1 Plasmid having a function of t-vector and expression vector, and expression of the target gene using the same 审中-公开
标题翻译：具有t载体和表达载体的功能的质粒以及使用其的靶基因的表达
公开(公告)号：US20060199185A1
公开(公告)日：2006-09-07
申请号：US10564880
申请日：2003-12-31
申请人： Young Song , Haryoung Poo , Moon-Hee Sung , Seung-Pyo Hong , Yoon Ho Choi , Kwang Kim , Il Han Lee , Je Hyun Park
发明人： Young Song , Haryoung Poo , Moon-Hee Sung , Seung-Pyo Hong , Yoon Ho Choi , Kwang Kim , Il Han Lee , Je Hyun Park
IPC分类号： C40B40/02 , C40B40/08 , C12Q1/68 , C12N15/74
CPC分类号： C12N15/64
摘要： The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the present invention relates to an expression vector having the target gene inserted into the plasmid, and the expression of the target gene using the same. The plasmid of the present invention can be converted into a vector that expresses the target protein by one-step T-vector cloning in a simple and rapid manner. The plasmid converted into the expression vector does not require a re-transformation step and allows the high-level expression of the target protein only by the culturing of transformed E. coli without the addition of an expensive inducer. Thus, according to the present invention, expression plasmids for large amounts of target genes can be produced at the same time, so that the present invention will be very efficient in establishing expression systems for certain genomes and gene groups.
摘要翻译：本发明涉及用作T载体和表达载体两者的质粒（pHCE-FOREX），其通过赋予来自组成型高水平表达载体的HCE启动子产生T载体功能而产生，并可表达目标蛋白以简单快速的方式。此外，本发明涉及将靶基因插入质粒的表达载体，以及使用该表达载体的靶基因的表达。本发明的质粒可通过简单快速的一步T载体克隆转化为表达靶蛋白的载体。转化成表达载体的质粒不需要再转化步骤，并且仅通过培养转化的大肠杆菌而不添加昂贵的诱导物才允许靶蛋白的高水平表达。因此，根据本发明，可以同时生产大量靶基因的表达质粒，从而本发明在建立某些基因组和基因组的表达系统方面将非常有效。

13. 发明申请

US20060127447A1 Poly-gamma-glutamate having ultra high molecular weight and method for using the same 审中-公开
标题翻译：具有超高分子量的聚-γ-谷氨酸盐及其使用方法
公开(公告)号：US20060127447A1
公开(公告)日：2006-06-15
申请号：US10520557
申请日：2003-07-10
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Kwang Seok Kim , Jae Jun Song , Eu Gene Rha , Kwang Kim , Kenji Soda , Makoto Ashiuchi , Ha Ryoung Poo , Seung Lee
IPC分类号： A61K38/16 , A61K47/00 , C07K14/32 , C12N1/21 , C12P21/06
CPC分类号： A61K9/0014 , A61K8/88 , A61K9/0095 , A61K47/42 , A61Q19/00 , C08H1/00 , C12P21/02
摘要： The present invention relates to a poly-gamma-glutamate (PGA) having an ultra-high molecular weight greater than 5,000 kDa. The ultra-high molecular weight PGA according to the present invention has a mean molecular weight greater than 13,000 kDa, and more than 95% of its molecules have a molecular weight ranging from 3,000 to 15,000 kDa. Also, it can be produced by the culturing of Bacillus subtilis var. chungkookjang. The ultra-high molecular weight PGA according to the present invention shows very excellent moisture-absorbing, moisture-retaining, sustained release, mineral solubility, and water-absorbing properties, and thus, can be used as a new and high value-added material in various applications.
摘要翻译：本发明涉及超高分子量大于5,000kDa的聚-γ-谷氨酸（PGA）。根据本发明的超高分子量PGA的平均分子量大于13000kDa，其分子的95％以上具有3,000至15,000kDa的分子量。此外，它可以通过培养枯草芽孢杆菌变种产生。 ung ko ang。根据本发明的超高分子量PGA显示出非常优异的吸湿性，保湿性，缓释性，矿物溶解性和吸水性，因此可用作新的高附加值材料在各种应用中。

14. 发明授权

US08080402B2 Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase 有权
标题翻译：使用编码聚-γ-谷氨酸合成酶的基因的肽P5和Anal3的表面表达方法
公开(公告)号：US08080402B2
公开(公告)日：2011-12-20
申请号：US11622158
申请日：2007-01-11
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Kyung Park , Chul-Joong Kim , Ha-Ryoung Poo
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Kyung Park , Chul-Joong Kim , Ha-Ryoung Poo
IPC分类号： C12N15/00 , C12N1/21 , A61K48/00
CPC分类号： C07K14/32
摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal 3 35.
摘要翻译：本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法，来自芽孢杆菌属的膜蛋白基因（pgsBCA）菌株并参与了聚-γ-谷氨酸的合成。此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。因此，本发明的方法具有非常高的工业实用性。此外，除了P5 3和Anal 3 35之外，本发明可以应用于其它肽抗生素。

15. 发明授权

US08618057B2 Anticoagulant and composition for preventing thrombus containing poly-gamma-glutamic acid 失效
标题翻译：用于预防含有聚-γ-谷氨酸的血栓的抗凝血剂和组合物
公开(公告)号：US08618057B2
公开(公告)日：2013-12-31
申请号：US13444324
申请日：2012-04-11
申请人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
发明人： Moon-Hee Sung , Chung Park , Seung-Pyo Hong , Haryoung Poo , Tae-Woo Kim
IPC分类号： A61K38/02
CPC分类号： A61K31/74 , A23L33/175 , A61K9/0095
摘要： The present invention relates to an anticoagulant and a composition for preventing thrombus formation, which contain poly-gamma-glutamic acid (PGA) as an active ingredient. The inventive PGA is a water-soluble, anionic, biodegradable and edible amino acid polymer material, which has an anticoagulant effect of preventing thrombi from being accumulated in blood vessels, shows an excellent sustained-release effect and is harmless to the human body. Thus, it is useful as a high-value-added anticoagulant and a food or beverage composition for preventing thrombus formation.
摘要翻译：本发明涉及含有聚-γ-谷氨酸（PGA）作为活性成分的用于预防血栓形成的抗凝血剂和组合物。本发明的PGA是一种水溶性，阴离子，可生物降解和可食用的氨基酸聚合物材料，其具有防止血栓在血管中积聚的抗凝作用，显示出优异的持续释放作用并对人体无害。因此，作为高附加值抗凝剂和用于预防血栓形成的食品或饮料组合物是有用的。

16. 发明申请

US20110312030A1 CONSISTENT HIGH EXPRESSION VECTOR CONTAINING REP E MUTATED GENE 有权
标题翻译：一致性高表达载体含有突变基因
公开(公告)号：US20110312030A1
公开(公告)日：2011-12-22
申请号：US13143918
申请日：2010-01-07
申请人： Moon-Hee Sung , Seung Pyo Hong , A Ri Jang
发明人： Moon-Hee Sung , Seung Pyo Hong , A Ri Jang
IPC分类号： C12P21/00 , C07K14/00 , C12N1/21 , C12N15/70 , C12N15/11 , C12N15/74
CPC分类号： C12N15/70 , C07K14/195 , C12N15/746
摘要： A vector for constitutively expressing a high level of a target protein, and more particularly a RepE mutant protein containing a deletion of 21 amino acids in the C-terminal region of a RepE protein and a vector for constitutively expressing a high level of a target protein, which comprises a gene encoding the mutant protein. The constitutive high-level expression vector can stably express a high level of a target protein. Also, the surface expression vector can express a target protein on the surface of recombinant microorganisms while constitutively expressing a high level of the target protein, and thus will be useful for construction of an antigen for vaccines.
摘要翻译：用于组成型表达高水平靶蛋白的载体，更具体地，在RepE蛋白的C-末端区域中含有21个氨基酸缺失的RepE突变蛋白和用于组成型表达高水平靶蛋白的载体，其包含编码突变蛋白的基因。组成型高水平表达载体可稳定表达高水平的靶蛋白。此外，表面表达载体可以在重组微生物的表面上表达靶蛋白，同时组成型表达高水平的靶蛋白，因此可用于构建疫苗抗原。

17. 发明授权

US07553636B2 Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector 有权
标题翻译：具有编码聚-γ-谷氨酸合成酶的基因的pgsBCA的表达表达载体，以及使用载体在微生物表面表达靶蛋白的方法
公开(公告)号：US07553636B2
公开(公告)日：2009-06-30
申请号：US10484605
申请日：2002-08-09
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Chul-Joong Kim , Kenji Soda , Makoto Ashiuchi
IPC分类号： C12N15/09 , C12N1/21 , C12N15/63
CPC分类号： C12N9/1051 , C07K2319/02 , C12N9/1288 , C12Y204/01133 , C12Y207/08005
摘要： The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms.
摘要翻译：本发明涉及具有pgsBCA的表达表达载体，编码聚-γ-谷氨酸合成酶的基因和使用该载体在微生物表面表达靶蛋白的方法。插入外来基因的载体转化微生物，使外源蛋白稳定表达在微生物表面。

18. 发明申请

US20080152615A1 Composition for Adjuvant Containing Poly-Gamma-Glutamic Acid 审中-公开
标题翻译：含有多糖 - 谷氨酸的佐剂组合物
公开(公告)号：US20080152615A1
公开(公告)日：2008-06-26
申请号：US11817021
申请日：2005-12-06
申请人： Moon-Hee Sung , Chul-Joong Kim , Ha Ryoung Poo , Seung Pyo Hong , Jong Soo Lee , Ji Youn Kim
发明人： Moon-Hee Sung , Chul-Joong Kim , Ha Ryoung Poo , Seung Pyo Hong , Jong Soo Lee , Ji Youn Kim
IPC分类号： A61K39/02 , A61K39/12
CPC分类号： A61K39/39 , A61K2039/55516
摘要： The present invention relates to a composition for an immunopotentiator (adjuvant) containing poly-gamma-glutamic acid and a composition for a vaccine containing the immunopotentiator, and more particularly, to an immunopotentiator containing poly-gamma-glutamic acid capable of enhancing antibody production rate by administering it to an animal together with antigen having low immunogenicity, and a composition for a vaccine containing the immunopotentiator and antigen. The inventive adjuvant has almost no toxicity and side effects, and show high antibody titer even when it is used with antigen having poor immunogenicity, so it can be used by adding to medical composition including preventive or curative vaccine for non-contagious chronic diseases as well as cancer, especially prostatic carcinoma, colon carcinoma, lung cancer, breast cancer, ovarian cancer, head and neck cancer, pudendum cancer, bladder cancer, brain tumor and glioma.
摘要翻译：本发明涉及含有聚-γ-谷氨酸的免疫增强剂（佐剂）组合物和含有免疫增强剂的疫苗组合物，更具体地说，涉及含有能够提高抗体产生率的聚-γ-谷氨酸的免疫增强剂通过将其与抗免疫原性低的抗原一起施用于动物，以及含有免疫增强剂和抗原的疫苗组合物。本发明的佐剂几乎没有毒副作用，即使与抗免疫原性差的抗原一起使用也显示出高的抗体效价，因此可以通过添加包含用于非传染性慢性疾病的预防或治疗性疫苗的医药组合物作为癌症，特别是前列腺癌，结肠癌，肺癌，乳腺癌，卵巢癌，头颈癌，pudendum癌，膀胱癌，脑肿瘤和胶质瘤。

19. 发明授权

US07255855B2 Surface expression method of peptides P5 and Anal3 using the gene encoding poly-gamma-glutamate synthetase 失效
标题翻译：使用编码聚-γ-谷氨酸合成酶的基因的肽P5和Anal3的表面表达方法
公开(公告)号：US07255855B2
公开(公告)日：2007-08-14
申请号：US10789164
申请日：2004-02-27
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Kyung Park , Chul-Joong Kim , Ha-Ryoung Poo
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Kyung Park , Chul-Joong Kim , Ha-Ryoung Poo
IPC分类号： A01N63/00 , C12N15/00 , C12N1/20
CPC分类号： C07K14/32
摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Ana13 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Ana13 43 expressed on their surface, and the use thereof.According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Ana13 35.
摘要翻译：本发明涉及一种用于表达具有两亲性的肽抗生素P5 3和Ana1335的方法，并且在微生物表面上显示出抗菌，抗真菌和抗癌活性61,63,65,67,69,71，使用含有外源的载体来自芽孢杆菌属的膜蛋白基因（pgsBCA）菌株并参与了聚-γ-谷氨酸的合成。此外，本发明涉及在其表面表达的各种肽抗生素P515和Ana1343的乳酸形成细菌及其用途。根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。因此，本发明的方法具有非常高的工业实用性。此外，本发明可以应用于除P5 3和Ana1335之外的其它肽抗生素。

20. 发明申请

US20050191720A1 Surface expression method of peptides P5 and ANAL3 using the gene encoding poly-gamma-glutamate synthetase 失效
标题翻译：使用编码聚-γ-谷氨酸合成酶的基因的肽P5和ANAL3的表达表达方法
公开(公告)号：US20050191720A1
公开(公告)日：2005-09-01
申请号：US10789164
申请日：2004-02-27
申请人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
发明人： Moon-Hee Sung , Seung-Pyo Hong , Jong-Su Lee , Chang-Min Jung , Kyung-Soo Hahm , Dong-Gun Lee , Yoon Park , Chul-Joong Kim , Ha-Ryoung Poo
IPC分类号： C07H21/04 , C07K14/32 , C07K16/30 , C12N1/21 , C12N15/74 , C12P21/06
CPC分类号： C07K14/32
摘要： The present invention relates to a method for expressing each of peptide antibiotics P5 3 and Anal3 35 having amphiphilicity and showing antibacterial, antifungal and anticancer activities 61, 63, 65, 67, 69, 71, on the microbial surface, using a vector containing outer membrane protein genes (pgsBCA) that are derived from Bacillus sp. strains and involved in the synthesis of poly-gamma-glutamate. Moreover, the present invention relates to lactic acid-forming bacteria having each of the peptide antibiotics P5 15 and Anal3 43 expressed on their surface, and the use thereof. According to the present invention, the peptide antibiotics can be expressed on the surface of various microorganisms transformed with the surface expression vectors. The inventive method for the surface expression of the peptide antibiotics allows the peptide antibiotics to be mass-produced without a purification process. Thus, the inventive method has very high industrial applicability. Further, the present invention can be applied to other peptide antibiotics besides P5 3 and Anal3 35.
摘要翻译：本发明涉及在微生物表面上表达具有两亲性的肽抗生素P5 3和Anal335各自表现出抗菌，抗真菌，抗癌活性的方法，来自芽孢杆菌属的膜蛋白基因（pgsBCA）菌株并参与了聚-γ-谷氨酸的合成。此外，本发明涉及在其表面表达的各肽抗生素P515和Anal343各自的乳酸形成细菌及其用途。根据本发明，肽抗生素可以在用表面表达载体转化的各种微生物的表面上表达。肽抗生素表面表达的本发明方法允许肽抗生素在没有纯化过程的情况下批量生产。因此，本发明的方法具有非常高的工业实用性。此外，本发明可以应用于除P5 3和Anal335之外的其它肽抗生素。

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