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11. 发明授权

US5811236A Electrochemiluminescent rhenium moieties and methods for their use 失效
标题翻译：电化学发光铼部分及其使用方法
公开(公告)号：US5811236A
公开(公告)日：1998-09-22
申请号：US468524
申请日：1995-06-06
申请人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
发明人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C07H21/04 , C07K15/28 , G01N23/53
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要： Electrochemiluminiscent moieties having the formula �Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.q (L.sup.5).sub.r (L.sup.6).sub.s !.sub.t (B).sub.u wherein P is a polydentate ligand of Re; L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 and L.sup.6 are ligands of Re, each of which may be the same as or different from each other ligand; B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5 or L.sup.6 ; m is an integer equal to or greater than 1; each of n, o, p, q, r and s is zero or an integer; t is an integer equal to or greater than 1; and u is an integer equal to or greater than 1; P, L.sup.1, L.sup.2, L.sup.3, L.sup.4, L.sup.5, L.sup.6 and B being of such composition and number that the chemical moiety can be induced to emit electromagnetic radiation and the total number of bonds to Re provided by the ligands of Re being equal to the coordination of Re are disclosed. Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are disclosed. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译：具有式[Re（P）m（L1）n（L2）o（L3）p（L4）q（L5）r（L6）s] t（B）u的电化学发光部分，其中P是Re的多齿配体; L1，L2，L3，L4，L5和L6是Re的配体，其各自可以彼此相同或不同; B是作为Re的配体或与P，L1，L2，L3，L4，L5或L6中的一个或多个相结合的物质; m是等于或大于1的整数; n，o，p，q，r和s中的每一个为零或整数; t是等于或大于1的整数; u是等于或大于1的整数; P，L1，L2，L3，L4，L5，L6和B具有这样的组成和数量，可以诱导化学部分发射电磁辐射，并且由Re的配体提供的Re的总数相等于 Re的协调被披露。公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量的电化学发光测定。这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

12. 发明授权

US06316607B1 Electrochemiluminescent assays 失效
标题翻译：电化学发光测定
公开(公告)号：US06316607B1
公开(公告)日：2001-11-13
申请号：US08472425
申请日：1995-06-07
申请人： Richard J. Massey , Michael J. Powell , Paul A. Mied , Peter Feng , Leopoldo Della Ciana , Walter J. Dressick , Mohindar S. Poonian
发明人： Richard J. Massey , Michael J. Powell , Paul A. Mied , Peter Feng , Leopoldo Della Ciana , Walter J. Dressick , Mohindar S. Poonian
IPC分类号： C12Q168
CPC分类号： G01N33/76 , C07D473/08 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , G01N33/533 , G01N33/569 , G01N33/582
摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译：提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。

13. 发明授权

US5229272A Catalytic antibody components 失效
标题翻译：催化抗体成分
公开(公告)号：US5229272A
公开(公告)日：1993-07-20
申请号：US498225
申请日：1990-03-23
申请人： Sudhir Paul , Michael J. Powell , Richard J. Massey , John H. Kenten
发明人： Sudhir Paul , Michael J. Powell , Richard J. Massey , John H. Kenten
IPC分类号： A61K39/395 , C07K1/113 , C07K1/22 , C07K14/00 , C07K14/52 , C07K14/54 , C07K14/55 , C07K14/575 , C07K14/705 , C07K16/00 , C07K16/06 , C07K16/26 , C07K16/44 , C07K19/00 , C12N9/00 , C12N9/48 , C12N15/09 , C12P21/02 , C12P21/08 , C12R1/19 , C12R1/91
CPC分类号： C07K14/57563 , C07K14/55 , C07K16/06 , C07K16/26 , C07K16/44 , C12N9/0002 , C12N9/48 , C07K2319/00 , C07K2319/75
摘要： Catalytic antibody components, methods for producing catalytic antibody components, methods for using catalytic antibody components, in particular, single chain and smaller components are disclosed. Catalytic antibody components able to promote the cleavage or formation of an amide, peptide, ester or glycosidic bond, and which are prepared from monoclonal catalytic antibodies, catalytic autoantibodies or by site-directed mutagenesis are disclosed. Methods of using catalytic antibody components alone or in combination with other antibody components or other biological moieties are disclosed.
摘要翻译：公开了催化抗体组分，用于制备催化抗体组分的方法，使用催化抗体组分的方法，特别是单链和较小组分。公开了能够促进切割或形成酰胺，肽，酯或糖苷键的催化抗体组分，其由单克隆催化抗体，催化自身抗体或通过定点诱变制备。公开了单独使用催化抗体组分或与其它抗体组分或其它生物学部分组合的方法。

14. 发明授权

US06916606B2 Electrochemiluminescent assays 失效
标题翻译：电化学发光测定
公开(公告)号：US06916606B2
公开(公告)日：2005-07-12
申请号：US10274079
申请日：2002-10-18
申请人： Richard J. Massey , Michael J. Powell , Paul A. Mied , Peter Feng , Leopoldo Della Ciana , Walter J. Dressick , Mohindar S. Poonian
发明人： Richard J. Massey , Michael J. Powell , Paul A. Mied , Peter Feng , Leopoldo Della Ciana , Walter J. Dressick , Mohindar S. Poonian
IPC分类号： C07D473/08 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C12Q1/70 , A61K38/00 , G01N33/53 , G01N33/566
CPC分类号： G01N33/76 , C07D473/08 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , G01N33/533 , G01N33/569 , G01N33/582
摘要： Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate.The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.
摘要翻译：提供了对多组分液体中存在感兴趣分析物的定性和定量的电化学发光测定。这些方法包括使样品与标示有电化学发光化学部分的试剂接触，并能够与感兴趣的分析物结合，将所得样品暴露于电化学能并检测由电化学发光化学部分发射的电磁辐射。还提供了用于检测和鉴定液体食物或食物匀浆中多种分析物的存在的方法。这些方法包括将设置有多种试剂的诊断试剂保持器浸入食物或食物匀浆中，从液体食物或食物匀浆中除去诊断试剂载体，以及检测和鉴定多种感兴趣分析物的存在到诊断试剂保持器，从而检测和鉴定食物或食物匀浆中感兴趣的多种分析物的存在。本发明还提供了大肠杆菌的酶免疫测定法。该测定包括将样品接种到用于大肠杆菌繁殖的合适的培养基中，将存在于培养基中的大肠杆菌固定在合适的表面上，用针对固定的大肠杆菌的抗体处理该表面并检测固定在合适的表面上的固定的大肠杆菌的存在。

15. 发明授权

US06881536B1 Particle based electrochemiluminescent assays 失效
标题翻译：基于粒子的电化学发光测定
公开(公告)号：US06881536B1
公开(公告)日：2005-04-19
申请号：US08413536
申请日：1995-03-30
申请人： Haresh P. Shah , Lee O. Hall , Michael J. Powell , Richard J. Massey
发明人： Haresh P. Shah , Lee O. Hall , Michael J. Powell , Richard J. Massey
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01L7/08 , G01L9/00 , G01N21/66 , G01N21/76 , G01N33/533 , G01N33/543 , G01N33/569 , G01N33/58 , G01N33/76 , G01N33/546 , G01N33/548 , G01N33/551
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , C12Q1/686 , G01L7/082 , G01L9/006 , G01N21/66 , G01N21/76 , G01N33/533 , G01N33/54313 , G01N33/54326 , G01N33/54366 , G01N33/5438 , G01N33/569 , G01N33/582 , G01N33/76 , Y10S436/805 , Y10S436/806
摘要： A method for the detection of an analyte of interest in a sample, which method comprises the steps of: (1) forming a composition comprising (a) a sample, (b) at least one substance selected from the group consisting of (i) added analyte of interest or an analog of the analyte of interest, (ii) a binding partner of the analyte of interest or its said analog, and (iii) a reactive component capable of binding with (i) or (ii), wherein one of said substances is linked to a label compound having a chemical moiety capable of being induced to luminesce, and (c) a plurality of particles capable of specifically binding with the analyte and/or a substance defined in (b) (i), (b) (ii), or (b) (iii); (2) inducing the label compound to luminesce; and (3) measuring luminescence emitted by the composition to determine the presence of the analyte of interest in the sample.
摘要翻译：一种用于检测样品中感兴趣分析物的方法，所述方法包括以下步骤：（1）形成组合物，其包含（a）样品，（b）至少一种选自（i）（ii）感兴趣分析物或其所述类似物的结合配偶体，和（iii）能够与（i）或（ii）结合的反应性成分，其中一个的所述物质与具有能够诱导发光的化学部分的标记化合物连接，和（c）能够与（b）（i），（i）所定义的分析物和/或物质特异性结合的多个粒子 b）（ii），或（b）（iii）; （2）诱导标签化合物发光; 和（3）测量由组合物发射的发光以确定样品中感兴趣的分析物的存在。

16. 发明授权

US06468741B1 Electrochemiluminescent rhenium moieties 失效
标题翻译：电化学发光铼部分
公开(公告)号：US06468741B1
公开(公告)日：2002-10-22
申请号：US09157788
申请日：1998-09-21
申请人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
发明人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo Della Ciana
IPC分类号： C12Q168
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要： A labeled substance comprising a biological substance linked to a luminescent rhenium-containing label. Qualitative and quantitative electrochemiluminescent assays using the same. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety containing rhenium and capable of combining with the analyte of interest, exposing the resulting sample to chemical, electrochemical, or electromagnetic energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety.
摘要翻译：包含与发光含铼标记物连接的生物物质的标记物质。使用相同的定性和定量电化学发光测定。这些方法包括使样品与含有铼的电化学发光化学部分标记的试剂接触并能够与感兴趣的分析物结合，将所得样品暴露于化学，电化学或电磁能并检测由电化学发光化学部分发射的电磁辐射。

17. 发明授权

US5591581A Electrochemiluminescent rhenium moieties and methods for their use 失效
标题翻译：电化学发光铼部分及其使用方法
公开(公告)号：US5591581A
公开(公告)日：1997-01-07
申请号：US227898
申请日：1994-04-15
申请人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo D. Ciana
发明人： Richard J. Massey , Michael J. Powell , Walter J. Dressick , Jonathan K. Leland , Janel K. Hino , Mohindar S. Poonian , Leopoldo D. Ciana
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/81
摘要： Electrochemiluminescent moieties having the formula(Re(P).sub.m (L.sup.1).sub.n (L.sup.2).sub.o (L.sup.3).sub.p (L.sup.4).sub.t (B).sub.uwhereinP is a polydentate ligand of Re;L.sup.1, L.sup.2, L.sup.3 and L.sup.4 are ligands of Re, each of which may be the same as or not the same as each other ligand;B is a substance which is a ligand of Re or is conjugated to one or more of P, L.sup.1, L.sup.2, L.sup.3 and L.sup.4 ;m is an integer equal to or greater than 1;each of n, o, p, q, r and s is zero or an integer;t is an integer equal to or greater than 1; andu is an integer equal to or greater than 1;P, L.sup.1, L.sup.2, L.sup.3, L.sup.4 and B being of such composition and number that the chemical moiety can be induced to electrochemiluminesce and the total number of bonds to Re provided by the ligands of Re being equal to the coordination number of Re are disclosed.Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids using these moieties are also disclosed.
摘要翻译：具有式（Re（P）m（L1）n（L2）o（L3）p（L4）t（B）u的电化学发光部分，其中P是Re; L1，L2，L3和L4的多齿配体， Re，其各自可以与每个其它配体相同或不相同; B是作为Re的配体或与P，L1，L2，L3和L4中的一个或多个相结合的物质; m是整数等于或大于1; n，o，p，q，r和s中的每一个为零或整数; t为等于或大于1的整数; u为等于或大于1的整数; P，L1，L2，L3，L4和B具有化学部分可以被诱导为电化学发光的组成和数量，并且Re的配体提供的Re的总数等于Re的配位数还公开了使用这些部分存在于多组分液体中的感兴趣分析物的定性和定量电化学发光测定。

18. 发明授权

US5763196A Assays using cross-linked polypeptide fragments of .beta.-galactosidase 失效
标题翻译：使用β-半乳糖苷酶的交联多肽片段进行测定
公开(公告)号：US5763196A
公开(公告)日：1998-06-09
申请号：US592029
申请日：1996-01-26
申请人： Michael J. Powell , Pyare Khanna , David Lingenfelter , Scott J. Eisenbeis
发明人： Michael J. Powell , Pyare Khanna , David Lingenfelter , Scott J. Eisenbeis
IPC分类号： C12Q1/37 , G01N33/53 , C12Q1/34
CPC分类号： C12Q1/37
摘要： Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
摘要翻译：在β-半乳糖苷酶的酶供体多肽片段中形成分子内交联，从而形成环状酶供体，其受到酶受体片段的互补阻碍形成β-半乳糖苷酶的活性。循环酶供体可以通过切割线性化以恢复互补能力。公开了其中这种环状酶供体由特异性分析物线性化的测定，以及式（IMAGE）其中R是羟基或乙酸酯的新型双官能双马来酰亚胺交联剂。

19. 发明申请

US20150176065A1 DETECTION OF PNA CLAMPING 有权
标题翻译：检测PNA钳位
公开(公告)号：US20150176065A1
公开(公告)日：2015-06-25
申请号：US14472240
申请日：2014-08-28
申请人： Michael J. Powell , Aiguo Zhang
发明人： Michael J. Powell , Aiguo Zhang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2549/126 , C12Q2565/518 , C12Q2565/519
摘要： This invention provides methods and systems for detecting sequence variants in a sample nucleic acid. Methods include PNA clamping/PCR using primers with binding moieties followed by capture and detection of amplicons on a solid support. Systems include PCR reagents with primers having binding moieties, a PNA clamping probe, a solid support capable of capturing the PCR amplicons incorporating the binding moieties, and a detector device.
摘要翻译：本发明提供用于检测样品核酸中的序列变体的方法和系统。方法包括使用具有结合部分的引物的PNA夹持/ PCR，然后在固体支持物上捕获和检测扩增子。系统包括具有结合部分的引物的PCR试剂，PNA夹持探针，能够捕获掺入结合部分的PCR扩增子的固体支持物和检测器装置。

20. 发明授权

US6140137A Conjugates and specific immunoassays for the methadone metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine 有权
标题翻译：美沙酮代谢物2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷的缀合物和特异性免疫测定
公开(公告)号：US6140137A
公开(公告)日：2000-10-31
申请号：US930959
申请日：1998-09-24
申请人： Gerald F. Sigler , Michael J. Powell , William A. Coty , Anthony J. Sanchez
发明人： Gerald F. Sigler , Michael J. Powell , William A. Coty , Anthony J. Sanchez
IPC分类号： C07D207/20 , C07D207/40 , C07D207/404 , C07D207/416 , G01N33/94 , G01N33/536 , C07K16/44 , C12N9/96
CPC分类号： C07D207/404 , C07D207/20 , C07D207/416 , G01N33/9486 , Y10S436/815
摘要： Novel chemical analogs of the methadone metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) are disclosed. The derivatives can be used for formation of EDDP-protein conjugates. The conjugates can be used in turn to raise antibodies reactive with EDDP and having a low cross-reactivity with methadone. The antibodies and EDDP-enzyme polypeptide conjugates provide the basis for specific immunoassays used in monitoring compliance with methadone treatment.
摘要翻译： PCT No.PCT / US97 / 17784 Sec。 371日期：1997年9月24日 102（e）1997年9月24日PCT PCT 1997年9月24日PCT公布。第WO98 / 54133号公报日期1998年12月3日公开了美沙酮代谢物2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）的新型化学类似物。该衍生物可用于形成EDDP-蛋白质缀合物。可以依次使用缀合物来提高与EDDP反应的抗体，并且与美沙酮具有低的交叉反应性。抗体和EDDP-酶多肽缀合物提供了用于监测美沙酮治疗依从性的特异性免疫测定的基础。

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