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11. 发明授权

US07674958B2 GRG23 and GRG51 genes conferring herbicide resistance 有权
标题翻译：赋予除草剂抗性的GRG23和GRG51基因
公开(公告)号：US07674958B2
公开(公告)日：2010-03-09
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

12. 发明申请

US20090227771A1 GRG23 AND GRG51 GENES CONFERRING HERBICIDE RESISTANCE 有权
标题翻译： GRG23和GRG51基因对抗药性的抵抗
公开(公告)号：US20090227771A1
公开(公告)日：2009-09-10
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C07K14/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

13. 发明申请

US20110236950A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
标题翻译： GRG23 EPSP合成：组合物和使用方法
公开(公告)号：US20110236950A1
公开(公告)日：2011-09-29
申请号：US12856082
申请日：2010-08-13
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

14. 发明申请

US20110252502A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
公开(公告)号：US20110252502A1
公开(公告)日：2011-10-13
申请号：US12856058
申请日：2010-08-13
申请人： Laura C. Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura C. Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： A01H5/10 , C12N15/63 , C12N1/21 , A01G1/00 , A01H5/00 , C12N9/00 , C12N15/82 , C12N15/52 , C12N5/10
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

15. 发明申请

US20110232179A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
公开(公告)号：US20110232179A1
公开(公告)日：2011-09-29
申请号：US12856072
申请日：2010-08-13
申请人： Laura C. Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura C. Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： A01G1/00 , C12N15/52 , C12N15/63 , C12N1/21 , C12N5/10 , A01H5/00 , A01H5/10 , C12N9/00 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

16. 发明授权

US08404934B2 Genes encoding nematode toxins 有权
标题翻译：编码线虫毒素的基因
公开(公告)号：US08404934B2
公开(公告)日：2013-03-26
申请号：US12638591
申请日：2009-12-15
申请人： Cheryl L. Peters , Brian Vande Berg , Brian Carr , Julia T. Daum , Vadim Beilinson , Sandra Volrath , Candace Poutre , Kimberly Sampson
发明人： Cheryl L. Peters , Brian Vande Berg , Brian Carr , Julia T. Daum , Vadim Beilinson , Sandra Volrath , Candace Poutre , Kimberly Sampson
IPC分类号： C12N1/21 , C12N15/32 , C12N15/82 , A01N37/18 , C07K14/325
CPC分类号： C12N15/8241 , C12N9/0059 , C12N9/0071 , C12N15/8285 , C12Y110/03001 , C12Y114/18001 , Y02A40/164
摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。提供了包含杀线虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀线虫剂核酸分子。另外，包含对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

17. 发明申请

US20110252503A1 Plants With Improved Nitrogen Utilization and Stress Tolerance 有权
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US20110252503A1
公开(公告)日：2011-10-13
申请号：US12916854
申请日：2010-11-01
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Philip Hammer , Laura Schouten
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Philip Hammer , Laura Schouten
IPC分类号： A01H5/00 , C12N5/10 , C12N1/21 , C12N15/54 , C07K16/16 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻增加产量的限制的转基因植物，或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物谷氨酸激酶（NAGK）基因，调节植物中的氮利用。本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。在各种实施方案中，载体构建体包括一个或多个包含SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

18. 发明申请

US20110030091A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
公开(公告)号：US20110030091A1
公开(公告)日：2011-02-03
申请号：US12856021
申请日：2010-08-13
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , C12N1/21 , C12N5/10 , A01H5/00 , C12N9/00 , A01N57/20
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

19. 发明申请

US20080313769A9 EPSP synthase domains conferring glyphosate resistance 审中-公开
标题翻译：赋予草甘膦抗性的EPSP合酶结构域
公开(公告)号：US20080313769A9
公开(公告)日：2008-12-18
申请号：US11651752
申请日：2007-01-10
申请人： Brian Carr , Philip Hammer , Todd Hinson , Brian Vande Berg
发明人： Brian Carr , Philip Hammer , Todd Hinson , Brian Vande Berg
IPC分类号： A01H1/00 , C07H21/04 , C12N9/10 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括新型EPSP合成酶和编码这种酶的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。新型蛋白质包含选自本文提供的结构域的至少一个序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合成酶。

20. 发明申请

US20110223647A1 GRG36: Novel EPSP Synthase Gene Conferring Herbicide Resistance 审中-公开
标题翻译： GRG36：新型EPSP合酶基因赋予除草剂抗性
公开(公告)号：US20110223647A1
公开(公告)日：2011-09-15
申请号：US13095972
申请日：2011-04-28
申请人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
发明人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
IPC分类号： C12N9/10
CPC分类号： C12N9/1085 , C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 6, 7, 9, 10, 12, 13, 15, 17, 18, 20, 21, or 23, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, 5, 8, 11, 14, 16, 19, or 22, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30932, B-30933, B-30934, B-30945, B-30946, B-30947, or B-30948, as well as variants and fragments thereof.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1,3,4,6,7,9,10,12,13,15,17,18,20,21中所示的核苷酸序列或23，编码SEQ ID NO：2,5,8,11,14,16,19或22所示的氨基酸序列的核苷酸序列，将存在细菌宿主中的除草剂抗性核苷酸序列作为登录号NRRL B-30932，B-30933，B-30934，B-30945，B-30946，B-30947或B-30948，以及其变体和片段。

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