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    • 12. 发明授权
    • 5-aminolevulinic acid producing microorganism and process for producing
5-aminolevulinic acid
    • 5-氨基乙酰丙酸生产微生物和生产5-氨基乙酰丙酸的方法
    • US5733770A
    • 1998-03-31
    • US575818
    • 1995-12-20
    • Keitaro WatanabeSeiji NishikawaTohru TanakaYasushi Hotta
    • Keitaro WatanabeSeiji NishikawaTohru TanakaYasushi Hotta
    • C12P13/00C12N1/20
    • C12P13/005C12R1/01C12R1/19C12R1/38C12R1/85Y10S435/822
    • A microorganism that produces 5-aminolevulinic acid, wherein the microorganism has a 5-aminolevulinic acid dehydratase variant having a reduced inhibitor constant for a 5-aminolevulinic acid dehydratate inhibitor. A microorganism that produces 5-aminolevulinic acid, wherein the microorganism is a photosynthetic bacterium that produces 5-aminolevulinic acid without light irradiation. A progess for producing 5-aminolevulinic acid comprising the step of culturing a microorganism that produces 5-aminolevulinic acid in a culture medium under at least one condition selected from the group consisting of (a) less than 1 ppm of dissolved oxygen concentration in the culture medium, (b) from -180 to 50 mV of oxidation-reduction potential in the culture medium, and (c) from 5.times.10.sup.-9 to (KrM-2.times.10.sup.-8) (mol of O.sub.2 /ml.multidot.min.multidot.cell) of cellular respiration rate, wherein KrM represents the maximum cellular respiration rate when oxygen is supplied in an excess quantity.
    • 一种产生5-氨基乙酰丙酸的微生物,其中微生物具有5-氨基乙酰丙酸脱水抑制剂的抑制剂常数降低的5-氨基乙酰丙酸脱水酶变体。 产生5-氨基乙酰丙酸的微生物,其中所述微生物是不照射光而产生5-氨基乙酰丙酸的光合细菌。 一种用于生产5-氨基乙酰丙酸的方法,包括在选自以下的至少一种条件下培养在培养基中产生5-氨基乙酰丙酸的微生物的步骤:(a)在培养物中小于1ppm的溶解氧浓度 培养基中,(b)在培养基中的-180至50mV的氧化还原电位,和(c)5×10 -9至(KrM-2×10 -8)(摩尔的O 2 / ml×Min×细胞)的细胞呼吸速率,其中 当以超量供给氧气时,KrM表示最大的细胞呼吸速率。