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11. 发明授权

US07230159B2 Isolated BOS1 gene promoters from arabidopsis and uses thereof 失效
标题翻译：来自拟南芥的分离的BOS1基因启动子及其用途
公开(公告)号：US07230159B2
公开(公告)日：2007-06-12
申请号：US10505744
申请日：2003-03-05
申请人： Tesfaye Mengiste , Xi Chen , John Salmeron , Robert Dietrich
发明人： Tesfaye Mengiste , Xi Chen , John Salmeron , Robert Dietrich
IPC分类号： C12N15/82 , C12N15/09 , A01H5/00
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8216 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to an isolated promoter sequence from Arabidopisis thaliana BOSI gene encoding a protein for biotic and abiotic stress tolerance. Also, the invention relates to recombinant vectors, expression cassettes, host cells, plants or progeny thereof comprising nucleic acid molecules operably linked to said promoter.
摘要翻译：本发明涉及编码用于生物和非生物胁迫耐受性的蛋白质的拟南芥BOSI基因的分离的启动子序列。此外，本发明涉及包含与所述启动子可操作地连接的核酸分子的重组载体，表达盒，宿主细胞，植物或其后代。

12. 发明申请

US20070048768A1 Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping and marker development 审中-公开
标题翻译：筛选基因特异性杂交多态性（GSHPs）的方法及其在基因定位和标记开发中的应用
公开(公告)号：US20070048768A1
公开(公告)日：2007-03-01
申请号：US11472789
申请日：2006-06-22
申请人： John Salmeron , Tong Zhu
发明人： John Salmeron , Tong Zhu
IPC分类号： C12Q1/68 , G06F19/00 , C12P19/34
CPC分类号： C12Q1/6827 , G16B25/00
摘要： A method for identification of gene specific hybridization polymorphisms (GSHPs) and their use is presented. The method involves the steps of a) global screening for hybridization polymorphisms using microarray; b) enzyme mediated genome complexity reduction; c) enzyme mediated differential signal amplification and noise reduction; d) data extraction and GSHP identification; and e) use of GSHPs in high throughput screening.
摘要翻译：提出了一种鉴定基因特异性杂交多态性（GSHPs）及其应用的方法。该方法包括以下步骤：a）使用微阵列全面筛选杂交多态性; b）酶介导的基因组复杂性降低; c）酶介导的差异信号放大和降噪; d）数据提取和GSHP鉴定; 和e）在高通量筛选中使用GSHPs。

13. 发明授权

US07049421B2 Trichothecene-resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US07049421B2
公开(公告)日：2006-05-23
申请号：US10614954
申请日：2003-07-07
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N15/09 , C12N15/31 , A01H5/00
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses isolated polynucleotides encoding polypeptides having trichothecene 3-O-acetyltransferase activity, recombinant vectors and host cell comprising said polynucleotides.
摘要翻译：本发明公开了编码具有单端孢霉素3-O-乙酰转移酶活性的多肽的分离的多核苷酸，重组载体和包含所述多核苷酸的宿主细胞。

14. 发明申请

US20050160491A1 Transgenic plants compromising nucleic acid molecules encoding RAR1 disease resistance proteins and uses thereof 失效
标题翻译：转基因植物损害编码RAR1抗病性蛋白质的核酸分子及其用途
公开(公告)号：US20050160491A1
公开(公告)日：2005-07-21
申请号：US11011906
申请日：2004-12-14
申请人： Manuel Sainz , John Salmeron
发明人： Manuel Sainz , John Salmeron
IPC分类号： C07K14/415 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8281 , C07K14/415 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.
摘要翻译：本发明涉及包含核酸分子的转基因植物，所述核酸分子包含编码参与抗病性的RAR1蛋白的核苷酸序列和RAR1多肽。本发明特别涉及改变转基因植物中编码RAR1蛋白的表达核酸分子以改变水平的抗病性的方法，以及改变增强的抗病性的转基因植物，后代和种子。本发明还涉及增强R抗性基因表达，抗病性信号转导基因，介导抗病性的基因或参与抗病性分子合成的方法。本发明还涉及通过增加本发明的核酸分子的表达来调节其它编码序列的表达的方法。

15. 发明申请

US20050120410A1 Nucleic acid sequences encoding the bos1 gene and promoter from arabidopisis thaliana and uses thereof 失效
标题翻译：编码bos1基因的核酸序列和来自阿拉伯糖的拟南芥的启动子及其用途
公开(公告)号：US20050120410A1
公开(公告)日：2005-06-02
申请号：US10505744
申请日：2003-03-05
申请人： Tesfaye Mengiste , Xi Chen , John Salmeron , Robert Dietrich
发明人： Tesfaye Mengiste , Xi Chen , John Salmeron , Robert Dietrich
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H1/00 , C07H21/04 , C12N9/24
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8216 , C12N15/8279 , C12N15/8282
摘要： The present invention pertains to nucleic acid molecules isolated from Arabidopisis thaliana comprising nucleotide sequences that encode proteins for biotic and abiotic stress tolerance. The invention particularly relates to methods of using nucleic acid molecules and/or proteins from Arabidopisis in transgenic plants to confer enhanced biotic and/or abiotic stress tolerance, and to use such nucleic acids to assist germplasm enhancement by breeding. In particular, the invention pertains to nucleic acid molecules isolated from Arabidopisis thaliana of the BOS1 cDNA and genomic gene and encoding the BOS1 polypeptide. The present invention also pertains to nucleic acid molecules isolated from Arabidopisis thaliana that encode a promoter from the BOS1 gene, and methods of using the promoter. Also, the invention relates to host cells, plants or progeny thereof comprising the nucleic acid molecules or recombinant molecules described herein.
摘要翻译：本发明涉及从拟南芥中分离的核酸分子，其包含编码用于生物和非生物胁迫耐受性的蛋白质的核苷酸序列。本发明特别涉及在转基因植物中使用来自Arabidopisis的核酸分子和/或蛋白质赋予增强的生物和/或非生物胁迫耐受性的方法，并且使用这样的核酸通过育种来辅助种质增殖。特别地，本发明涉及从BOS1 cDNA和基因组基因的拟南芥中分离的编码BOS1多肽的核酸分子。本发明还涉及从编码来自BOS1基因的启动子的拟南芥中分离的核酸分子以及使用该启动子的方法。此外，本发明涉及包含本文所述的核酸分子或重组分子的宿主细胞，植物或其后代。

16. 发明授权

US06346655B1 Trichothecne-Resistant transgenic plants 有权
标题翻译：抗逆转录病毒转基因植物
公开(公告)号：US06346655B1
公开(公告)日：2002-02-12
申请号：US09538414
申请日：2000-03-29
申请人： Thomas M. Hohn , Cheryl Peters , John Salmeron
发明人： Thomas M. Hohn , Cheryl Peters , John Salmeron
IPC分类号： C12N1509
CPC分类号： C07H21/04 , C12N9/1029 , C12N15/8209 , C12N15/8242 , C12N15/8274 , C12N15/8282
摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.
摘要翻译：本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性。如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。在一个具体实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移至单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基蓖麻酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。

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