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11. 发明授权

US07745189B2 Regulatable growth of filamentous fungi 有权
标题翻译：丝状真菌的可调节生长
公开(公告)号：US07745189B2
公开(公告)日：2010-06-29
申请号：US12184211
申请日：2008-07-31
申请人： Ali R. Akin , Elizabeth A. Bodie , Shirley M. Burrow , Nigel Dunn-Coleman , Geoffrey Turner , Michael Ward
发明人： Ali R. Akin , Elizabeth A. Bodie , Shirley M. Burrow , Nigel Dunn-Coleman , Geoffrey Turner , Michael Ward
IPC分类号： C12N9/12 , C12N15/00 , C12P21/06 , C12P19/34 , C07H21/04
CPC分类号： C12N9/1205 , C07K14/38 , C12N15/80 , C12P21/02
摘要： The present invention generally relates to hyphal growth in fungi and in particular describes the modulation of genes associated with hyphal growth in filamentous fungi. The present invention provides methods and systems for the production of proteins and/or chemicals from filamentous fungi which comprise modulation of genes associated with hyphal growth. Specifically, the present invention is directed to a full length cotA gene, its gene product and methods of use.
摘要翻译：本发明通常涉及真菌中的菌丝生长，并且具体地描述了丝状真菌中菌丝生长相关基因的调节。本发明提供了用于从丝状真菌生产蛋白质和/或化学物质的方法和系统，其包括调节与菌丝生长相关的基因。具体地，本发明涉及全长cotA基因，其基因产物和使用方法。

12. 发明授权

US5683911A Method of enhancing the delignification and/or bleaching of pulp 失效
标题翻译：增加纸浆脱木质素和/或漂白的方法
公开(公告)号：US5683911A
公开(公告)日：1997-11-04
申请号：US426505
申请日：1995-04-21
申请人： Elizabeth A. Bodie , William A. Cuevas , Marja Koljonen
发明人： Elizabeth A. Bodie , William A. Cuevas , Marja Koljonen
IPC分类号： A23K1/165 , C12N9/24 , C12P21/00 , C12R1/00 , C12R1/645 , C12S3/08 , D21C5/00 , D21C9/10 , C12N9/26
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , Y10S426/807 , Y10S435/822
摘要： The present invention relates to novel xylanases purified from the microorganism, Microtetraspora flexuosa. Each biochemically unique xylanase is thermostable and has optimal activity in alkaline conditions. The present invention is also directed to methods that utilize either the whole enzyme system or one or more of the above purified thermostable xylanases obtained from Microtetraspora flexuosa. These enzymes are excellent candidates for enhancing the delignification and bleaching of pulp. Furthermore, treating the pulp with the above enzymes prior to bleaching may reduce the amount of chlorine containing and/or peroxide chemicals required in the bleaching process.
摘要翻译：本发明涉及从微生物Microtetraspora flexuosa中纯化的新型木聚糖酶。每种生物化学独特的木聚糖酶是耐热的，并且在碱性条件下具有最佳的活性。本发明还涉及利用从Microtetraspora flexuosa获得的整个酶系统或一种或多种上述纯化的热稳定性木聚糖酶的方法。这些酶是增强纸浆脱木质素和脱色的极好候选物。此外，在漂白之前用上述酶处理纸浆可以减少漂白过程中所需的含氯和/或过氧化物的量。

13. 发明授权

US08481321B2 Compositions and methods for improved protein production 有权
公开(公告)号：US08481321B2
公开(公告)日：2013-07-09
申请号：US13494889
申请日：2012-06-12
申请人： Elizabeth A. Bodie , Steve Sungjin Kim
发明人： Elizabeth A. Bodie , Steve Sungjin Kim
IPC分类号： C12N15/80
摘要： The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.

14. 发明申请

US20120244576A1 COMPOSITIONS AND METHODS FOR IMPROVED PROTEIN PRODUCTION 失效
标题翻译：用于改进蛋白质生产的组合物和方法
公开(公告)号：US20120244576A1
公开(公告)日：2012-09-27
申请号：US13494889
申请日：2012-06-12
申请人： Elizabeth A. Bodie , Steve Kim
发明人： Elizabeth A. Bodie , Steve Kim
IPC分类号： C12N1/15 , C12N15/11 , C12N15/80 , C12P21/00
CPC分类号： C12N15/80 , C07K14/37 , C12N9/2437 , C12N15/01 , C12P21/00 , C12P21/02 , Y02P20/52
摘要： The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.
摘要翻译：本发明涉及在影响蛋白质产生的宿主细胞中鉴定新的核酸序列（本文称为7p，8k，7E，9G，8Q和203）。本发明还提供具有编码7p，8k，7E，9G，8Q和203的基因的部分或全部的突变或缺失的宿主细胞，其在图1中示出。 1，分别为SEQ ID NO：1-6。本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。

15. 发明授权

US4442128A Production of fermented whey products containing a thickening polymer 失效
标题翻译：生产含增稠聚合物的发酵乳清产品
公开(公告)号：US4442128A
公开(公告)日：1984-04-10
申请号：US284421
申请日：1981-07-20
申请人： Robert D. Schwartz , Elizabeth A. Bodie
发明人： Robert D. Schwartz , Elizabeth A. Bodie
IPC分类号： A23C21/02 , C12P19/06 , C12N1/14 , C12R1/64
CPC分类号： C12R1/64 , A23C21/02 , C12P19/06 , Y10S435/91
摘要： Dairy whey, a waste product of chesse production, is fermented with an organism to produce a whey product containing a thickening polymer that serves as a thickening agent. Fermentation is carried out by forming a fermentation broth of whey and glucose, and optionally a water soluble phosphate and/or yeast extract and then fermenting the broth with Xanthomonas campestris ATCC 31922. The resultant fermented whey product is used as a thickening agent in the food industry.
摘要翻译：乳制品乳清是一种生产的废物，用生物体发酵，生产含有增稠剂的增稠剂的乳清产品。通过形成乳清和葡萄糖的发酵液以及任选的水溶性磷酸盐和/或酵母提取物，然后用野油菜黄单孢菌ATCC 31922发酵肉汤来进行发酵。所得发酵乳清产品用作食品中的增稠剂行业。

16. 发明授权

US08741654B2 Compositions and methods for improved protein production 失效
标题翻译：用于改善蛋白质生产的组合物和方法
公开(公告)号：US08741654B2
公开(公告)日：2014-06-03
申请号：US13494889
申请日：2012-06-12
申请人： Elizabeth A. Bodie , Steve Sungjin Kim
发明人： Elizabeth A. Bodie , Steve Sungjin Kim
IPC分类号： C12N15/80
CPC分类号： C12N15/80 , C07K14/37 , C12N9/2437 , C12N15/01 , C12P21/00 , C12P21/02 , Y02P20/52
摘要： The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.
摘要翻译：本发明涉及在影响蛋白质产生的宿主细胞中鉴定新的核酸序列（本文称为7p，8k，7E，9G，8Q和203）。本发明还提供具有编码7p，8k，7E，9G，8Q和203的基因的部分或全部的突变或缺失的宿主细胞，其在图1中示出。 1，分别为SEQ ID NO：1-6。本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。

17. 发明授权

US08304221B2 Regulatable growth of filamentous fungi 失效
标题翻译：丝状真菌的可调节生长
公开(公告)号：US08304221B2
公开(公告)日：2012-11-06
申请号：US12816362
申请日：2010-06-15
申请人： Ali R. Akin , Elizabeth A. Bodie , Shirley M. Burrow , Nigel Dunn-Coleman , Geoffrey Turner , Michael Ward
发明人： Ali R. Akin , Elizabeth A. Bodie , Shirley M. Burrow , Nigel Dunn-Coleman , Geoffrey Turner , Michael Ward
IPC分类号： C12N9/12 , C12N15/00 , C12N1/00 , C12P21/06 , C12P19/34 , C07H21/04 , C07K1/00
CPC分类号： C12N9/1205 , C07K14/38 , C12N15/80 , C12P21/02
摘要： The present invention generally relates to hyphal growth in fungi and in particular describes the modulation of genes associated with hyphal growth in filamentous fungi. The present invention provides methods and systems for the production of proteins and/or chemicals from filamentous fungi which comprise modulation of genes associated with hyphal growth. Specifically, the present invention is directed to a full length cotA gene, its gene product and methods of use.
摘要翻译：本发明通常涉及真菌中的菌丝生长，并且具体地描述了丝状真菌中菌丝生长相关基因的调节。本发明提供了用于从丝状真菌生产蛋白质和/或化学物质的方法和系统，其包括调节与菌丝生长相关的基因。具体地，本发明涉及全长cotA基因，其基因产物和使用方法。

18. 发明申请

US20100129835A1 Methods for Selecting Improved Strains 审中-公开
标题翻译：选择改良菌株的方法
公开(公告)号：US20100129835A1
公开(公告)日：2010-05-27
申请号：US12522671
申请日：2008-01-08
申请人： Elizabeth A. Bodie
发明人： Elizabeth A. Bodie
IPC分类号： G01N33/53
CPC分类号： C12N15/01 , C12N15/1034
摘要： The present invention relates to a system for improving a strain of filamentous fungus that is useful in producing an enzyme of interest such as a cellulase. The invention involves generating genetic diversity in a strain of fungal cells, subjecting the population of cells to selection, and isolating the cells that overproduce the enzyme. The selection techniques involve culturing the population of cells in purified cellulose and/or at an elevated temperature that is adverse to production of the enzyme.
摘要翻译：本发明涉及一种用于改善丝状真菌菌株的系统，其可用于生产感兴趣的酶如纤维素酶。本发明涉及在真菌细胞株中产生遗传多样性，对细胞群进行选择，并分离过量产生酶的细胞。选择技术包括在纯化的纤维素中和/或在不利于酶的生成的升高的温度下培养细胞群。

19. 发明授权

US06399329B1 Phenol oxidizing enzymes 有权
标题翻译：苯酚氧化酶
公开(公告)号：US06399329B1
公开(公告)日：2002-06-04
申请号：US09468578
申请日：1999-12-21
申请人： Huaming Wang , Elizabeth A. Bodie
发明人： Huaming Wang , Elizabeth A. Bodie
IPC分类号： C12P2106
CPC分类号： C12N9/0004 , C11D3/38654 , Y10S435/911
摘要： The invention relates to phenol oxidizing enzymes encoded by nucleic acids capable of hybridizing to the nucleic acid having the sequence shown in SEQ ID NO: 1 and in particular phenol oxidizing enzymes obtainable from fungus. Particularly provided are nucleic acid sequences and amino acids from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum, and expression vectors and host cells comprising said nucleic acid sequences encoding phenol oxidizing enzymes. Additionally, methods for producing the phenol oxidizing enzymes as well as methods for constructing expression hosts are disclosed.
摘要翻译：本发明涉及能够与具有SEQ ID NO：1所示序列的核酸杂交的核酸编码的酚氧化酶，特别是可从真菌获得的酚氧化酶。特别提供的是来自Bipolaris spicifera，Curvularia pallescens和Amerosporium atrum的核酸序列和氨基酸，以及包含编码酚氧化酶的所述核酸序列的表达载体和宿主细胞。此外，公开了生产酚氧化酶的方法以及构建表达宿主的方法。

20. 发明授权

US5437992A Five thermostable xylanases from microtetraspora flexuosa for use in delignification and/or bleaching of pulp 失效
标题翻译：来自微曲霉的五种热稳定的木聚糖酶用于纸浆的脱木质素和/或漂白
公开(公告)号：US5437992A
公开(公告)日：1995-08-01
申请号：US234338
申请日：1994-04-28
申请人： Elizabeth A. Bodie , William A. Cuevas , Marja Koljonen
发明人： Elizabeth A. Bodie , William A. Cuevas , Marja Koljonen
IPC分类号： A23K1/165 , C12N9/24 , C12P21/00 , C12R1/00 , C12R1/645 , C12S3/08 , D21C5/00 , D21C9/10 , C07G17/00 , C12N1/12 , D21C3/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , Y10S426/807 , Y10S435/822
摘要： Five xylanases are purified from the microorganism, Microtetraspora flexuosa,preferably strain ATCC 35864. Each biochemically unique xylanase is thermostable and has optimal activity in alkaline conditions. These enzymes are excellent candidates for enhancing the delignification and bleaching of pulp. Furthermore, treating the pulp with the above enzymes prior to bleaching may reduce the amount of chlorine containing and/or peroxide chemicals required in the bleaching process.
摘要翻译：从微生物Microtetraspora flexuosa，优选菌株ATCC 35864纯化五种木聚糖酶。每种生物化学独特的木聚糖酶是耐热性的并且在碱性条件下具有最佳活性。这些酶是增强纸浆脱木质素和脱色的极好候选物。此外，在漂白之前用上述酶处理纸浆可以减少漂白过程中所需的含氯和/或过氧化物的量。

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