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    • 12. 发明授权
    • Biochip for the detection of phosphorylation and the detection method using the same
    • 用于检测磷酸化的生物芯片及其检测方法
    • US07794964B2
    • 2010-09-14
    • US12333383
    • 2008-12-12
    • Sang-Hyun ParkKyong Cheol Ko
    • Sang-Hyun ParkKyong Cheol Ko
    • C12Q1/48C07K14/00
    • C12Q1/485
    • The present invention relates to a biochip for detecting phosphorylation and a method for detecting phosphorylation using the same, more precisely a biochip prepared by integrating a protein produced from the recombination of a substrate of kinase selected from the group consisting of PKC (Protein Kinase C), cdc2-PK (cdc2 Protein Kinase) and DNA-PK (DNA-dependent Protein Kinase) and the elevated protein such as Selenomonas ruminantium membrane protein on a matrix surface coated with an active group, a kit for detecting phosphorylation composed of the said biochip and a cofactor labeled with a radio-isotope and a method for detecting phosphorylation using the same. The biochip for detecting phosphorylation of the present invention using a radio-isotope facilitates the detection of phosphorylation with a minimum amount of a sample by simple processes, compared with the conventional method using an antibody. Since this method can analyze a large amount of samples in a shorter period of time, it can be effectively used for the analysis of kinase activity.
    • 本发明涉及用于检测磷酸化的生物芯片和使用该生物芯片检测磷酸化的方法,更确切地说,通过将从激酶底物的重组产生的蛋白质整合而制备的生物芯片,所述蛋白质底物选自PKC(蛋白激酶C) ,cdc2-PK(cdc2蛋白激酶)和DNA-PK(DNA依赖性蛋白激酶)和升高的蛋白质如硒化单胞菌反应物膜蛋白在包被活性基团的基质表面上,用于检测由所述生物芯片组成的磷酸化试剂盒 和用放射性同位素标记的辅助因子和使用其的磷酸化检测方法。 与使用抗体的常规方法相比,使用放射性同位素检测本发明的磷酸化的生物芯片通过简单的方法促进了用最小量的样品的磷酸化检测。 由于该方法可以在更短的时间内分析大量样品,因此可以有效地用于激酶活性的分析。