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11. 发明授权

US08545686B2 Electrophoretic apparatus and electrophoretic method 有权
标题翻译：电泳仪和电泳法
公开(公告)号：US08545686B2
公开(公告)日：2013-10-01
申请号：US11397890
申请日：2006-04-05
申请人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
发明人： Ryoji Inaba , Tomoyuki Sakai , Motohiro Yamazaki , Takashi Gomi , Kazumichi Imai
IPC分类号： G01N27/453 , G01N27/447
CPC分类号： G01N27/44721 , G01N27/44782
摘要： The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.
摘要翻译：本发明涉及通过将激发光照射到多个电泳路径上并且将电泳路径输出的荧光以大致垂直于电泳方向的方向分散的方式来检测发射光谱。根据本发明，由于要检测的发射光谱随时间基本上不变化，因此可以使观察到的发射光谱完全对应于各种荧光染料或各种基底。

12. 发明申请

US20090134030A1 Capillary Electrophoretic Instrument and Capillary Array Assembly 有权
标题翻译：毛细管电泳仪器和毛细管阵列组件
公开(公告)号：US20090134030A1
公开(公告)日：2009-05-28
申请号：US12359115
申请日：2009-01-23
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译：通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

13. 发明授权

US07014746B2 Capillary electrophoretic instrument and capillary array assembly 有权
标题翻译：毛细管电泳仪和毛细管阵列组件
公开(公告)号：US07014746B2
公开(公告)日：2006-03-21
申请号：US10413540
申请日：2003-04-15
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/453
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries.By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.
摘要翻译：通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

14. 发明授权

US06325908B1 Electrophoresis analysis apparatus and sample vessel used therefor 失效
标题翻译：电泳分析装置及其使用的样品容器
公开(公告)号：US06325908B1
公开(公告)日：2001-12-04
申请号：US09132323
申请日：1998-08-11
申请人： Kazumichi Imai , Osamu Uchida
发明人： Kazumichi Imai , Osamu Uchida
IPC分类号： G01N2726
摘要： A multi-capillary type electrophoresis analysis apparatus has a sample tray for containing a plurality of samples, wherein part of the sample tray is made from a conductive material. Samples are introduced by applying a high voltage from a high voltage power supply between the sample tray and a coupler in a state in which one-ends of the capillaries are inserted in the samples contained in the sample tray. The apparatus eliminates the necessity of individually inserting electrodes in a plurality of samples contained in the sample tray, thereby making easy works for analysis.
摘要翻译：多毛细管型电泳分析装置具有用于容纳多个样品的样品托盘，其中，样品托盘的一部分由导电材料制成。通过在样品托盘和耦合器之间施加来自高电压电源的高电压，在毛细管的一端插入样品盘中包含的样品的状态下引入样品。该装置消除了在包含在样品托盘中的多个样品中单独插入电极的必要性，从而易于进行分析。

15. 发明授权

US5365559A Particle counting apparatus for a total counting of particles contained in a liquid sample 失效
标题翻译：用于对包含在液体样品中的颗粒的总计数的颗粒计数装置
公开(公告)号：US5365559A
公开(公告)日：1994-11-15
申请号：US8984
申请日：1993-01-26
申请人： Yu-Ming Hsueh , Kazumichi Imai , Masataka Koga
发明人： Yu-Ming Hsueh , Kazumichi Imai , Masataka Koga
IPC分类号： G01N15/14 , G01N15/02 , G01N29/00 , G06M1/10 , G06M11/00 , G01N15/00 , G01N21/53
CPC分类号： G01N15/0205 , G06M1/101 , G06M11/00
摘要： A particle counting apparatus for counting a total particle counting value contained in a liquid sample comprising, a flow cell in which the liquid sample flows, a light source for irradiating a light beam to the sample liquid in the flow cell, a detector for detecting pulse-wise signal scattered from the particles by the irradiating of the light beam, and a computer for obtaining the total particle counting value contained in the liquid sample as a counting value measured when the top end of the liquid sample is irradiated with the light beam and the plural particles in the top end does not overlapped in the light beam, multiplied by a coefficient.
摘要翻译：一种粒子计数装置，用于计数包含在液体样品中的总颗粒计数值，该液体样品包括：液体样品流动的流动池，用于向流动池中的样品液体照射光束的光源;检测脉冲的检测器通过照射光束从颗粒散射的信号;以及计算机，用于获得液体样品中包含的总颗粒计数值作为当用光束照射液体样品的顶端时测量的计数值，以及顶端的多个颗粒在光束中不重叠，乘以系数。

16. 发明申请

US20140200162A1 METHOD AND APPARATUS FOR NUCLEIC ACID ANALYSIS 审中-公开
标题翻译：核酸分析方法与装置
公开(公告)号：US20140200162A1
公开(公告)日：2014-07-17
申请号：US14233256
申请日：2012-05-16
申请人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
发明人： Toshiro Saito , Koshin Hamasaki , Satoshi Takahashi , Muneo Maeshima , Kyoko Imai , Kazumichi Imai , Ryuji Tao
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
摘要翻译：提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

17. 发明授权

US08201659B2 Recoilless speaker system 有权
标题翻译：无声扬声器系统
公开(公告)号：US08201659B2
公开(公告)日：2012-06-19
申请号：US12738819
申请日：2008-04-21
申请人： Kazumichi Imai , Yasue Imai
发明人： Kazumichi Imai , Yasue Imai
IPC分类号： H04R1/02
CPC分类号： H04R1/2811 , H04R1/227 , H04R1/288 , H04R2209/027
摘要： The present invention provides a recoilless speaker system capable of reducing adversely affecting vibration and generating an accurate and strong sound, and contributing to the realization of lighter weight, miniaturization and lower cost related to manufacturing, and also capable of being installed in a suspended state and generating sound even under zero gravity as long as air exists.The present invention includes a symmetrical and tubular resonance wall and a pair of or two or more pairs of vibration units symmetrically arranged on both left and right sides of the resonance wall, where the vibration units that form a pair are configured to vibrate synchronously with each other, the resonance wall is made from a flexible material so as to resonate to the vibration, a sound absorbing member is arranged in a tubular form along the inner wall of the resonance wall, and vibration suppressing materials are held at the sound absorbing member and/or the vibration unit.
摘要翻译：本发明提供一种能够减少不利地影响振动并产生准确而强烈的声音的无后坐力的扬声器系统，并且有助于实现与制造相关的更轻的重量，小型化和更低的成本，并且还能够以悬挂状态安装，只要空气存在，即使在零重力下也能产生声音。本发明包括对称和管状共振壁以及一对或两对或更多对振动单元，对称地布置在共振壁的左右两侧，其中形成一对的振动单元被配置为与每个振动单元同步地振动另一方面，谐振壁由柔性材料制成，从而与振动共振，吸声构件沿共振壁的内壁布置成管状，振动抑制材料保持在吸声构件上， /或振动单元。

18. 发明申请

US20100294588A1 RECOILLESS SPEAKER SYSTEM 有权
标题翻译：可重现扬声器系统
公开(公告)号：US20100294588A1
公开(公告)日：2010-11-25
申请号：US12738819
申请日：2008-04-21
申请人： Kazumichi Imai , Yasue Imai
发明人： Kazumichi Imai , Yasue Imai
IPC分类号： H04R7/00 , G10K13/00
CPC分类号： H04R1/2811 , H04R1/227 , H04R1/288 , H04R2209/027
摘要： The present invention provides a recoilless speaker system capable of reducing adversely affecting vibration and generating an accurate and strong sound, and contributing to the realization of lighter weight, miniaturization and lower cost related to manufacturing, and also capable of being installed in a suspended state and generating sound even under zero gravity as long as air exists.The present invention includes a symmetrical and tubular resonance wall and a pair of or two or more pairs of vibration units symmetrically arranged on both left and right sides of the resonance wall, where the vibration units that form a pair are configured to vibrate synchronously with each other, the resonance wall is made from a flexible material so as to resonate to the vibration, a sound absorbing member is arranged in a tubular form along the inner wall of the resonance wall, and vibration suppressing materials are held at the sound absorbing member and/or the vibration unit.
摘要翻译：本发明提供一种能够减少不利地影响振动并产生准确而强烈的声音的无后坐力的扬声器系统，并且有助于实现与制造相关的更轻的重量，小型化和更低的成本，并且还能够以悬挂状态安装，只要空气存在，即使在零重力下也能产生声音。本发明包括对称和管状共振壁以及一对或两对或更多对振动单元，对称地布置在共振壁的左右两侧，其中形成一对的振动单元被配置为与每个振动单元同步地振动另一方面，谐振壁由柔性材料制成，从而与振动共振，吸声构件沿共振壁的内壁布置成管状，振动抑制材料保持在吸声构件上， /或振动单元。

19. 发明申请

US20060086612A1 Capillary electrophoretic instrument and capillary array assembly 审中-公开
公开(公告)号：US20060086612A1
公开(公告)日：2006-04-27
申请号：US11296487
申请日：2005-12-08
申请人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
发明人： Muneo Maeshima , Kazumichi Imai , Masaya Kojima , Satoshi Takahashi , Hiromi Yamashita
IPC分类号： G01N27/447
CPC分类号： G01N27/44713 , G01N27/447 , G01N27/44704 , G01N27/44743 , G01N27/44782
摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

20. 发明申请

US20060006066A1 Electrophoresis apparatus, and method of analysis 有权
标题翻译：电泳仪及分析方法
公开(公告)号：US20060006066A1
公开(公告)日：2006-01-12
申请号：US11175297
申请日：2005-07-07
申请人： Motohiro Yamazaki , Kazumichi Imai
发明人： Motohiro Yamazaki , Kazumichi Imai
IPC分类号： B01D57/02 , G01N27/453
CPC分类号： G01N27/44743
摘要： Analysis is performed efficiently in an electrophoresis apparatus while avoiding deterioration of samples. A plurality of sample plates are stored in frozen storage. When a particular sample plate is being analyzed, other sample plates are stored in a standby unit. In this way, a plurality of sample plates can be stored under cool conditions and analysis can be performed efficiently.
摘要翻译：在电泳装置中有效地进行分析，同时避免样品的劣化。将多个样品板储存在冷冻储存器中。当分析特定样品板时，其他样品板储存在备用单元中。以这种方式，可以在冷却条件下存储多个样品板，并且可以有效地进行分析。

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