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11. 发明授权

US06737235B1 Nucleic acid isolation 失效
标题翻译：核酸分离
公开(公告)号：US06737235B1
公开(公告)日：2004-05-18
申请号：US08945731
申请日：1997-11-10
申请人： Philippe Cros , Abdelhamid Elaissari , Claude Mabilat , Christian Pichot , Marc Rodrigue , Lise Santoro
发明人： Philippe Cros , Abdelhamid Elaissari , Claude Mabilat , Christian Pichot , Marc Rodrigue , Lise Santoro
IPC分类号： C07H2104
CPC分类号： C12N15/101 , C12N15/1006 , C12Q1/6806
摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2 M is achieved.
摘要翻译：公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。该方法包括一种吸附剂制备步骤（a），其包括由水性连续相组成的溶胶和包含通过聚合（1）第一水溶性丙烯酰胺或丙烯酰胺衍生物单体制备的官能化颗粒聚合物的分散颗粒基质相（ 2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解度温度（LCST）。接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。

12. 发明授权

US5786147A Detection of enterobacteria 失效
标题翻译：检测肠杆菌
公开(公告)号：US5786147A
公开(公告)日：1998-07-28
申请号：US717526
申请日：1996-09-17
申请人： Claude Mabilat , Raoult Didier
发明人： Claude Mabilat , Raoult Didier
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/689
摘要： The invention relates to a single-stranded oligonucleotide chosen from oligonucleotides having a sequence of at least 12 consecutive nucleotide units which is included in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 52, and from the oligonucleotides complementary to these oligonucleotides, and to the applications of this oligonucleotide for detecting enterobacteria.
摘要翻译：本发明涉及选自具有至少12个连续核苷酸单元的序列的寡核苷酸的单链寡核苷酸，其包含在SEQ ID NO：1至SEQ ID NO：52中的一个序列中，以及与这些寡核苷酸互补的寡核苷酸，以及该寡核苷酸检测肠杆菌的应用。

13. 发明申请

US20050069890A1 Method for the detection and/or identification of the original animal species in animal matter contained in a sample 审中-公开
标题翻译：用于检测和/或鉴定样品中包含的动物物质中的原始动物物种的方法
公开(公告)号：US20050069890A1
公开(公告)日：2005-03-31
申请号：US10500646
申请日：2003-01-10
申请人： Claude Mabilat , Sabine Desvarenne , Odile Babola , Bruno Lacroix , Natalia Bello Pigem
发明人： Claude Mabilat , Sabine Desvarenne , Odile Babola , Bruno Lacroix , Natalia Bello Pigem
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68 , C12Q1/6876
CPC分类号： C12Q1/6876 , C12Q2600/16
摘要： Disclosed is a method for detecting or identifying the original animal species in a sample likely to contain an ingredient obtained at least from said species. The inventive method is characterized by the following steps: a) a nuclear fraction is obtained from said sample; b) at least one reactant which is specific to the animal species is provided and selected among a group formed by: - the reference sequences SEQ ID numbers 1 to 232, 242 to 261; - the sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; - the sequences homologous to each of the sequences SEQ ID numbers 1 to 232, 242 to 261 and sequences complementing each of the sequences SED ID numbers 1 to 232, 242 to 261, respectively; c) the nuclear fraction and said reactant are reacted with each other; and d) any signal or information resulting from the specific reaction between said reactant and the nuclear fraction is detected, whereby it can be established if said sample contains said original animal species.
摘要翻译：公开了一种用于检测或鉴定可能含有至少从所述物种获得的成分的样品中的原始动物物种的方法。本发明的方法的特征在于以下步骤：a）从所述样品获得核级分; b）至少一种对动物物种具有特异性的反应物，由选自下组形成的基团： - 参考序列SEQ ID号1至232,242至261; - 分别与每个序列SED ID号1至232,242至261互补的序列; - 与SEQ ID号1至232,242至261中的每一个序列同源的序列和分别与每个序列SED ID编号1至232,242至261互补的序列; c）核部分和所述反应物彼此反应; 并且d）检测由所述反应物和核部分之间的特异性反应产生的任何信号或信息，由此如果所述样品包含所述原始动物物种可以确定。

14. 发明授权

US5770362A Genomic DNA fragment of Streptococcus pneumoniae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae 失效
标题翻译：肺炎链球菌基因组DNA片段，杂交探针，扩增引物，肺炎链球菌检测试剂及方法
公开(公告)号：US5770362A
公开(公告)日：1998-06-23
申请号：US485180
申请日：1995-06-07
申请人： Jean-Pierre Claverys , Claude Mabilat
发明人： Jean-Pierre Claverys , Claude Mabilat
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The invention relates to a fragment of the genomic DNA of Strepeococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.
摘要翻译：本发明涉及肺炎链球菌的基因组DNA的片段，能够与肺炎链球菌的基因组DNA特异性杂交的探针，用于扩增，聚合的特异性引物，基因组DNA，试剂和方法与探针一起使用，以及任选的引物用于特异性检测生物样品中的肺炎链球菌。本发明的探针是与肺炎链球菌的基因组DNA的共有序列的至少一部分具有至少70％同源性的核苷酸序列，该共有序列选自核苷酸序列SEQ ID NO：2，SEQ ID NO：，SEQ ID NO 4，以及它们各自的互补序列。

15. 发明授权

US5695926A Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support 失效
标题翻译：使用非共价结合疏水性支持物的非常短的捕获探针的三明治杂交测定
公开(公告)号：US5695926A
公开(公告)日：1997-12-09
申请号：US255892
申请日：1994-06-07
申请人： Philippe Cros , Patrice Allibert , Fran.cedilla.ois Mallet , Claude Mabilat , Bernard Mandrand
发明人： Philippe Cros , Patrice Allibert , Fran.cedilla.ois Mallet , Claude Mabilat , Bernard Mandrand
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6813 , C12Q1/6834
摘要： A procedure for detecting single-stranded nucleotide sequences in a sample is disclosed wherein a passively fixed capture probe is used in concert with a non-radioactively labelled detection probe in a sandwich hybridization technique.
摘要翻译：公开了一种用于检测样品中的单链核苷酸序列的方法，其中在夹心杂交技术中使用被动固定的捕获探针与非放射性标记的检测探针一致。

16. 发明授权

US5656432A Genomic DNA fragment of Streptococcus pneumoniae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae 失效
标题翻译：肺炎链球菌基因组DNA片段，杂交探针，扩增引物，肺炎链球菌检测试剂及方法
公开(公告)号：US5656432A
公开(公告)日：1997-08-12
申请号：US486137
申请日：1995-06-07
申请人： Jean-Pierre Claverys , Claude Mabilat
发明人： Jean-Pierre Claverys , Claude Mabilat
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The invention relates to a fragment of the genomic DNA of Streptococcus pneumoniae, a probe capable of specifically hybridizing with the genomic DNA of Streptococcus pneumoniae, a specific primer for the amplification, by polymerization, of the genomic DNA, a reagent and a method which are used with the probe and, optionally, the primer, for specifically detecting Streptococcus pneumoniae in a biological sample. The probe of the invention is a nucleotide sequence having at least 70% homology with at least a portion of a consensus sequence of the genomic DNA of Streptococcus pneumoniae, this consensus sequence being chosen from the nucleotide sequences SEQ ID NO 2, SEQ ID NO 3, SEQ ID NO 4, which are identified in the description, and their respective complementary sequences.
摘要翻译：本发明涉及肺炎链球菌的基因组DNA片段，能够与肺炎链球菌的基因组DNA进行特异性杂交的探针，用于扩增，通过聚合的特异性引物，基因组DNA，试剂和方法与探针一起使用，以及任选的底漆，用于特异性检测生物样品中的肺炎链球菌。本发明的探针是与肺炎链球菌的基因组DNA的共有序列的至少一部分具有至少70％同源性的核苷酸序列，该共有序列选自核苷酸序列SEQ ID NO：2，SEQ ID NO：，SEQ ID NO 4，以及它们各自的互补序列。

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