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    • 13. 发明授权
    • Peptidase substrates
    • 肽酶底物
    • US08535902B2
    • 2013-09-17
    • US13386750
    • 2010-07-27
    • Olivier FabregaArthur JamesSylvain OrengaJohn PerryVindhya SalwaturaStephen Stanforth
    • Olivier FabregaArthur JamesSylvain OrengaJohn PerryVindhya SalwaturaStephen Stanforth
    • C12Q1/37C07K4/00C07D213/00
    • C12Q1/37C12Q1/04
    • The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: according to which: Y1 is a peptide, H or an alkyl; W1, W2, W3 and W4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination thereof; n=0, 1 or 2; X is NR, CZ5Z6, S or O, R being H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl, Z5 and Z6 being an alkyl; Y is N or N+R, R being alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z1, Z2, Z3 and Z4 being independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof.
    • 本发明涉及下式(I)的化合物用于检测肽酶活性和/或pH变化的用途:根据该化合物:Y1是肽,H或烷基; W1,W2,W3和W4独立地为H,Br,Cl,F,I,烷基,烷氧基,硫代甲基,全氟烷基,硝基,氰基,羧基(包括其酯或酰胺)或其任何组合; n = 0,1或2; X是NR,CZ5Z6,S或O,R是H,烷基,芳烷基,芳基,烷酰基或烷基磺酰基,Z5和Z6是烷基; Y是N或N + R,R是烷基,芳烷基,芳基,烷酰基或烷基磺酰基; Z 1,Z 2,Z 3和Z 4独立地为H,Br,Cl,F,I,烷基,芳基,烷氧基,全氟烷基,硝基,氰基,羧基,磺酰基,包括羧基或磺酰基酯或酰胺及其盐。