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11. 发明授权

US09481871B2 Nucleic acid encoding a polpeptide having aminotransferase activity, vectors and host cells comprising the nucleic acid 有权
标题翻译：编码具有氨基转移酶活性的多肽的核酸，载体和包含该核酸的宿主细胞
公开(公告)号：US09481871B2
公开(公告)日：2016-11-01
申请号：US13876412
申请日：2011-09-28
申请人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Akiko Nishi , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C07H21/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/10 , C12P41/00 , C12P17/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译：提供了一种从酮化合物廉价有效地制备用作药物制剂，农药等的中间体的旋光性氨基化合物的方法。具体而言，对于产生具有93％以上光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，作为氨基供体的谷氨酸比L-丙氨酸具有较高活性的多肽，本发明还提供了编码该基因的基因，以及高水平表达该基因的转化体。

12. 发明授权

US09267116B2 Amino acid dehydrogenase, and process for producing L-amino acid, 2-oxo acid or D-amino acid 有权
标题翻译：氨基酸脱氢酶，以及L-氨基酸，2-氧代酸或D-氨基酸的制备方法
公开(公告)号：US09267116B2
公开(公告)日：2016-02-23
申请号：US13133444
申请日：2009-12-08
申请人： Hiroyuki Kanamaru , Ryuuji Miki , Makoto Ueda , Hironori Nanba , Yoshihiko Yasohara
发明人： Hiroyuki Kanamaru , Ryuuji Miki , Makoto Ueda , Hironori Nanba , Yoshihiko Yasohara
IPC分类号： C12N9/06 , C12N1/21 , C12P13/04 , C07H21/04 , C12P7/40 , C12P13/22 , C12P41/00 , C12N15/70 , C12N15/74
CPC分类号： C12N9/0016 , C12N9/0012 , C12N15/70 , C12N15/74 , C12P7/40 , C12P13/04 , C12P13/22 , C12P41/006
摘要： The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.
摘要翻译：本发明涉及新的氨基酸脱氢酶，编码该酶的DNA，以及引入其中的DNA的转化体。本发明还涉及生产L-氨基酸，2-氧代酸或D-氨基酸的方法，其包括使氨基酸脱氢酶或能够产生酶的微生物或转化体作用于底物化合物。氨基酸脱氢酶即使对于具有大的侧链的氨基酸或2-氧代酸，例如含芳香环的基团，该酸与常规氨基酸脱氢酶的反应性差。氨基酸脱氢酶能够廉价且高效地生产有用的光学活性氨基酸。

13. 发明授权

US09029113B2 Modified aminotransferase, gene thereof, and method for producing optically active amino compound using same 有权
标题翻译：修饰的氨基转移酶及其基因，以及使用其制备光学活性氨基化合物的方法
公开(公告)号：US09029113B2
公开(公告)日：2015-05-12
申请号：US14004293
申请日：2012-03-09
申请人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10 , C12P17/12 , C12P13/00 , C12P13/04 , C12P41/00
CPC分类号： C12N9/1096 , C12P13/001 , C12P13/04 , C12P41/006
摘要： Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
摘要翻译：提供一种从酮化合物有效地制备用作药物，农药等的中间体的光学活性氨基化合物的方法。提供：通过修饰衍生自荧光假单胞菌的氨基转移酶，与野生型酶相比，具有增加立体选择性，耐热性和耐胺性化合物的氨基转移酶活性的多肽; 编码该多肽的基因; 以及高水平表达基因的转化体。

14. 发明授权

US08664373B2 Method for producing sophorose lipid 有权
标题翻译：生产槐糖脂的方法
公开(公告)号：US08664373B2
公开(公告)日：2014-03-04
申请号：US13126532
申请日：2009-10-23
申请人： Satohiro Yanagisawa , Shigeru Kawano , Yoshihiko Yasohara
发明人： Satohiro Yanagisawa , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C07H15/04 , C07H15/10
CPC分类号： C07H15/10 , A61K8/60 , A61K31/7016 , A61Q19/00 , C07H15/04 , C11C3/04 , C12P19/44 , C12R1/72
摘要： The present invention provides a low-cost, efficient method for producing a glycolipid biosurfactant, in particular, lactonic sophorose lipids. This method is characterized by culturing a microorganism capable of producing the biosurfactant under limited oxygen supply. The present invention enables preferential production of lactonic sophorose lipids and facilitates recovery of the lactonic sophorose lipids in a solid form. Further, the present invention enables production of high purity acidic sophorose lipids by hydrolyzing high purity lactonic sophorose lipids produced by the above method. The present invention also provides lactonic sophorose lipids that possess strong antibacterial and antifungal activities, and an antibacterial and/or antifungal agent containing the sophorose lipids.
摘要翻译：本发明提供了制备糖脂生物表面活性剂，特别是内酯槐糖脂的低成本，有效的方法。该方法的特征在于培养能够在有限供氧下产生生物表面活性剂的微生物。本发明能够优先生产内酯槐糖脂，并有利于以固体形式回收内酯槐糖脂。此外，本发明能够通过水解由上述方法生产的高纯度内酯槐糖脂来生产高纯度酸性槐糖脂。本发明还提供具有强抗菌和抗真菌活性的内酯槐糖脂，以及含有槐糖脂的抗菌和/或抗真菌剂。

15. 发明申请

US20130210092A1 Novel Transaminase, Gene Encoding the Same, and Method Using Them 有权
标题翻译：新型转氨酶，编码基因的方法和使用它们的方法
公开(公告)号：US20130210092A1
公开(公告)日：2013-08-15
申请号：US13876400
申请日：2011-09-28
申请人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
发明人： Toru Nagasawa , Toyokazu Yoshida , Kouichi Ishida , Noriyuki Ito , Shigeru Kawano , Yoshihiko Yasohara
IPC分类号： C12N9/10
CPC分类号： C12N9/1096 , C12P17/10 , C12P41/006 , C12Y206/01 , Y02P20/52
摘要： A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.
摘要翻译：提供了从酮化合物有效地制备用作药物制剂，农药等的中间体的光学活性氨基化合物的方法。具体地说，对水溶性有机溶剂具有高抗性的多肽和具有93％以上的光学纯度的（S）-1-苄基-3-吡咯烷酮的新型转氨酶活性，编码该多肽的基因，以及转化体本文还提供了表达高水平的基因。

16. 发明授权

US08304216B2 Method for production of erythro-or threo-2-amino-3-hydroxypropionic acid ester, novel carbonyl reductase, gene for the reductase, vector, transformant, and method for production of optically active alcohol using those 有权
标题翻译：用于生产红血球或苏氨酸-2-氨基-3-羟基丙酸酯的方法，新的羰基还原酶，还原酶的基因，载体，转化体和使用这些的生产光学活性醇的方法
公开(公告)号：US08304216B2
公开(公告)日：2012-11-06
申请号：US12225683
申请日：2007-03-29
申请人： Tozo Nishiyama , Yoshihiko Yasohara
发明人： Tozo Nishiyama , Yoshihiko Yasohara
IPC分类号： C12P7/02 , C12P7/62 , C07H21/04
CPC分类号： C12P13/04 , C12N9/0006
摘要： The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
摘要翻译：本发明的目的是提供一种制备赤式或苏氨酸-2-氨基-3-羟基丙酸酯的方法等。本发明涉及：通过使微生物的细胞在其上作用而不对称地还原N-2-氨基-3-氧代丙酸酯的方法; 具有不对称还原羰基化合物的活性的多肽，得到从Brevundimonas属的微生物分离的光学活性醇; 编码该多肽的DNA; 和产生多肽的转化体。本发明还涉及通过在多肽或转化体的帮助下还原羰基化合物来制备光学活性醇的方法。

17. 发明授权

US08133705B2 Aminotransferase, gene encoding the same, and method of using them 有权
标题翻译：氨基转移酶，编码基因的基因及其使用方法
公开(公告)号：US08133705B2
公开(公告)日：2012-03-13
申请号：US11920842
申请日：2006-05-22
申请人： Noriyuki Ito , Yoshihiko Yasohara
发明人： Noriyuki Ito , Yoshihiko Yasohara
IPC分类号： C12P13/00 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P41/006 , C12N9/1096 , C12P13/001 , C12P13/002 , C12P13/008 , C12P13/04 , C12P13/06 , C12P17/10
摘要： The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
摘要翻译：本发明涉及一种新的氨基转移酶，编码该酶的DNA，其中引入了DNA的重组载体和其中已导入载体的转化体。此外，本发明还涉及利用酶或转化体制备光学活性氨基化合物的方法。本发明的氨基转移酶具有将酮化合物，特别是环酮化合物有效转化为旋光性氨基化合物的能力。根据本发明，提供了有效制备光学活性氨基化合物，特别是旋光性环状氨基化合物的方法。

18. 发明申请

US20110236955A1 ARYLACYLAMIDASE GENE AND METHOD OF USING THE SAME 审中-公开
标题翻译： ARYLACYLAMIDASE基因及其使用方法
公开(公告)号：US20110236955A1
公开(公告)日：2011-09-29
申请号：US11576560
申请日：2005-09-26
申请人： Noriyuki Kizaki , Noriyuki Ito , Yoshihiko Yasohara , Toru Nagasawa
发明人： Noriyuki Kizaki , Noriyuki Ito , Yoshihiko Yasohara , Toru Nagasawa
IPC分类号： C12N9/80 , C12N15/55 , C12N15/63 , C12N1/21
CPC分类号： C12N9/80
摘要： The present invention provides an efficient method of producing an arylacylamidase. In an aspect, the present invention provides (a) a DNA comprising the base sequence shown in the sequence listing under SEQ ID NO:1, or (b) a DNA capable of hybridizing, under stringent conditions, with a DNA comprising the base sequence complementary to the base sequence shown in the sequence listing under SEQ ID NO:1 and coding for a polypeptide having arylacylamidase activity. In another aspect, the invention provides a polypeptide encoded by the DNA mentioned above and having arylacylamidase activity, a vector containing the DNA mentioned above, a transformant which is transformed using the DNA or the vector mentioned above, and a method for producing an arylacylamidase which comprises cultivating the transformant mentioned above and obtaining the arylacylamidase from the culture medium.
摘要翻译：本发明提供了生产芳基酰胺酶的有效方法。一方面，本发明提供（a）包含SEQ ID NO：1中的序列表中所示的碱基序列的DNA，或（b）能够在严格条件下与包含碱基序列的DNA杂交的DNA 与SEQ ID NO：1中的序列表中所示的碱基序列互补，并编码具有芳基酰胺酶活性的多肽。另一方面，本发明提供由上述DNA编码并具有芳基酰胺酶活性的多肽，含有上述DNA的载体，使用上述DNA或载体转化的转化体及其制备方法，包括培养上述转化体并从培养基中获得芳酰胺酰胺酶。

19. 发明授权

US07915022B2 Carbonyl reductase, gene thereof and method of using the same 有权
标题翻译：羰基还原酶，其基因及其使用方法
公开(公告)号：US07915022B2
公开(公告)日：2011-03-29
申请号：US12840026
申请日：2010-07-20
申请人： Noriyuki Kizaki , Miho Yano , Masashiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
发明人： Noriyuki Kizaki , Miho Yano , Masashiro Funaki , Teruaki Takesue , Yoshihiko Yasohara , Souichi Morikawa , Takahisa Nakai , Michihiko Kataoka , Sakayu Shimizu
IPC分类号： C12N9/02 , C07K14/00 , C12P21/00 , C12P7/16 , C12P7/02 , C12Q1/26
CPC分类号： C12P13/02 , C12N9/0006 , C12P41/002
摘要： The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant.Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
摘要翻译：本发明涉及具有不对称降低（3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁酮的活性以产生（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4- 从属于Ogataea的微生物分离的丁氧基羰基氨基-4-苯基-2-丁醇，编码多肽的DNA和产生多肽的转化体。本发明还涉及利用多肽或转化体制备（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇的方法。使用本发明的多肽或转化体，可以有效地制备光学活性醇如（2R，3S）-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇等。

20. 发明申请

US20100285544A1 Novel aminotransferase, gene encoding the same, and method of using them 有权
标题翻译：新型氨基转移酶，编码基因的基因及其使用方法
公开(公告)号：US20100285544A1
公开(公告)日：2010-11-11
申请号：US11920842
申请日：2006-05-22
申请人： Noriyuki Ito , Yoshihiko Yasohara
发明人： Noriyuki Ito , Yoshihiko Yasohara
IPC分类号： C12P13/00 , C07H21/04 , C12N15/63 , C12N1/00 , C12N9/10
CPC分类号： C12P41/006 , C12N9/1096 , C12P13/001 , C12P13/002 , C12P13/008 , C12P13/04 , C12P13/06 , C12P17/10
摘要： The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
摘要翻译：本发明涉及一种新的氨基转移酶，编码该酶的DNA，其中引入了DNA的重组载体和其中已导入载体的转化体。此外，本发明还涉及利用酶或转化体制备光学活性氨基化合物的方法。本发明的氨基转移酶具有将酮化合物，特别是环酮化合物有效转化为旋光性氨基化合物的能力。根据本发明，提供了有效制备光学活性氨基化合物，特别是旋光性环状氨基化合物的方法。

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