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    • 11. 发明授权
    • Cloned factor C cDNA of the Singapore Horseshoe Crab, Carcinoscorpius
rotundicauda and purification of Factor C proenzyme
    • 新加坡马蹄蟹,Carcinoscorpius rotundicauda的克隆因子C cDNA和因子C酶的纯化
    • US5712144A
    • 1998-01-27
    • US460521
    • 1995-06-02
    • Jeak Ling DingBow Ho
    • Jeak Ling DingBow Ho
    • C12N9/50C12N15/55C12Q1/37
    • C12N9/6408C12Y304/21084G01N33/579
    • Full-length and deletion subclones of cDNAs for Factor C of Carcinoscorpius rotundicauda are provided. These cDNAs have been cloned into .lambda.gt 22 and pGEM 11Zf(+). Further manipulations of the 5' and 3' ends of these cDNAs have been carried out, and these cDNAs have been further subcloned into other expression vectors such as pGEMEX-1, pET 3b, and the yeast shuttle vectors YEpsec 1 and pEMBLyex 4, and pPIC 9 and pHIL D2. Also provided are host cells transformed with expression vectors containing DNA molecules encoding proteins having Factor C-like enzymatic activity, methods of producing such proteins, methods for purifying Factor C zymogens, and methods for protecting Factor C zymogens from autoactivation by Gram negative bacterial endotoxin while the proenzyme is being purified and/or processed from amoebocyte lysates or from recombinant clones, or during storage or subsequent handling. This protection is afforded by the addition of 5-30% Me.sub.2 SO, which reversibly inhibits the Factor C zymogen.
    • 提供了番茄猕猴桃因子C的全长和缺失亚克隆。 这些cDNA已经克隆到λgt22和pGEM11Zf(+)中。 已经进行了这些cDNA的5'和3'端的进一步操作,并将这些cDNA进一步亚克隆到其他表达载体如pGEMEX-1,pET 3b和酵母穿梭载体YEpsec 1和pEMBLyex 4中,以及 pPIC 9和pHIL D2。 还提供了用含有编码具有因子C样酶活性的蛋白质的DNA分子转化的宿主细胞,产生这种蛋白质的方法,产生这种蛋白质的方法,纯化因子C酶原的方法,以及保护因子C酶原从革兰氏阴性细菌内毒素自动激活的方法, 正在从变形细胞裂解物或重组克隆纯化和/或加工,或在储存或后续处理过程中。 这种保护是通过加入5-30%的Me2SO,可逆地抑制因子C酶原来提供的。
    • 18. 发明授权
    • Sushi peptide multimer
    • 寿司肽多聚体
    • US07763704B2
    • 2010-07-27
    • US10563551
    • 2004-07-02
    • Jeak Ling DingBow Ho
    • Jeak Ling DingBow Ho
    • A61K38/00C07K14/00
    • C12Y304/21084A61K38/00C12N9/6408Y10S435/975Y10S530/81Y10S930/28
    • Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimic other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.
    • 内毒素也被称为脂多糖(LPS),是革兰氏阴性细菌感染引起的败血性休克的主要介质。 衍生自因子C的Sushi3结构域的化学合成的S3肽,其是鲎凝血级联的内毒素敏感的丝氨酸蛋白酶,其结合并中和LPS活性。 荧光标记的S3显示检测含LPS细菌。 为了大规模生产S3并模拟其他病原体识别分子,构建了S3基因的串联多聚体并在大肠杆菌中表达。 S3的四聚体显示出增强的对LPS诱导的活性的抑制作用。 基于S3的四聚体的亲和基质也显示出在去除LPS时特别有效。
    • 20. 发明授权
    • Heat-activated adhesive tape having an acrylic foam-like backing
    • 具有丙烯酸泡沫状背衬的热活化胶带
    • US06841234B2
    • 2005-01-11
    • US09920182
    • 2001-08-01
    • Ramesh LhilaKwang-Yol ParkKaila DelpozzoJian Ling Ding
    • Ramesh LhilaKwang-Yol ParkKaila DelpozzoJian Ling Ding
    • C09J7/02B32B7/12
    • C09J7/26C09J2201/128C09J2201/606C09J2201/61C09J2433/006C09J2477/003Y10T428/249982Y10T428/249983Y10T428/249984Y10T428/249985Y10T428/28Y10T428/2817Y10T428/2878Y10T428/2896
    • Heat-activated adhesive tape has a backing comprising 88-92% acrylic polymer containing: 35-45% of a first alkyl acrylate monomer with alkyl groups containing 4 to 12 carbon atoms, 30-40% of a second alkyl acrylate monomer with alkyl groups containing 4 to 12 carbon atoms, 6-10% a first monoethylenically unsaturated polar copolymerizable monomer, 1-2% a second monoethylenically unsaturated polar copolymerizable monomer, 0.3-0.5% photoinitiator, 1-2% filler, 0.05-0.07% crosslinker/chain extender, and 8-12% hollow glass microspheres; and heat-activated adhesive layer on at least one side. A pressure-sensitive adhesive may be on the other side. A method for preparing the adhesive tape involves partially polymerizing an oligomer, forming a coating composition, coating the composition onto a heat-activated adhesive disposed on a liner with a second liner, which may have a heat-activated adhesive, contiguously covering the first liner, and polymerizing the composition on the liners between two banks of ultraviolet light while cooling to form a tape.
    • 热活化胶带具有背衬,其包含88-92%的丙烯酸聚合物,其含有:含有含有4至12个碳原子的烷基的第一丙烯酸烷基酯单体的35-45%,具有烷基的第二烷基丙烯酸酯单体的30-40% 含有4至12个碳原子,6-10%的第一单烯属不饱和极性可共聚单体,1-2%第二单烯属不饱和极性可共聚单体,0.3-0.5%光引发剂,1-2%填料,0.05-0.07%交联剂/链 和8-12%的中空玻璃微球; 和至少一侧的热活化粘合剂层。 压敏粘合剂可以在另一侧。 制备粘合带的方法包括部分聚合低聚物,形成涂料组合物,将该组合物涂布到设置在具有第二衬垫的衬里上的热活化粘合剂上,该第二衬垫可具有热活化粘合剂,其邻接地覆盖第一衬垫 并且在冷却时将组合物聚合在两组紫外线之间的衬垫上以形成带。