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11. 发明申请

US20110144308A1 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译： N-GLYCANS在植物中的人类化和优化的组合物和方法
公开(公告)号：US20110144308A1
公开(公告)日：2011-06-16
申请号：US12955611
申请日：2010-11-29
申请人： LYNN F. DICKEY , KEVIN M. COX , CHARLES G. PEELE
发明人： LYNN F. DICKEY , KEVIN M. COX , CHARLES G. PEELE
IPC分类号： C07K16/00
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of α1,3-fucosyltransferase (FucT) and β1,2-xylosyltransferase (XylT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a “humanized” N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the G0 glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括向植物中引入一种重组构建体，其在植物中提供抑制α1,3-岩藻糖基转移酶（FucT）和（b），1,2-木糖基转移酶（XylT）的表达。这些构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产，而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了具有基本上均匀的糖基化曲线并且对于G0糖型基本均匀的糖蛋白组合物，包括单克隆抗体组合物。

12. 发明授权

US07659445B2 Expression of plasminogen and microplasminogen in duck weed 失效
标题翻译：在鸭杂草中表达纤溶酶原和微量淀粉样蛋白
公开(公告)号：US07659445B2
公开(公告)日：2010-02-09
申请号：US11056621
申请日：2005-02-11
申请人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
发明人： David Spencer , Lynn F. Dickey , John R. Gasdaska , Xiaowei Wang , Kevin M. Cox , Charles G. Peele
IPC分类号： C12N15/82 , C12N15/12 , C12N15/62
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

13. 发明申请

US20090060921A1 GLYCAN-OPTIMIZED ANTI-CD20 ANTIBODIES 审中-公开
标题翻译： GLYCAN优化的抗CD20抗体
公开(公告)号：US20090060921A1
公开(公告)日：2009-03-05
申请号：US12115133
申请日：2008-05-05
申请人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
发明人： Lynn F. Dickey , Kevin M. Cox , Charles G. Peele , Ming-Bo Wang
IPC分类号： A61K39/395 , C07K16/18 , C12N5/04 , A61P31/00
CPC分类号： C07K14/47 , C07K16/00 , C07K16/2878 , C07K16/2887 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Glycan-optimized monoclonal antibodies that specifically bind CD20 antigen and which have improved effector function are provided. The anti-CD20 antibodies of the invention have a glycosylation pattern that results in an antibody composition having predominately the G0 glycoform, and thus comprise N-glycans that lack fucose (i.e., afucosylated) and galactose residues attached thereto. In some embodiments, these anti-CD20 antibodies comprise the light chain and heavy chain sequences of the rituximab anti-CD20 antibody, and thus represent afucosylated rituximab. Methods for producing these glycan-optimized anti-CD20 antibodies are also provided.
摘要翻译：提供特异性结合CD20抗原并具有改善的效应子功能的糖基化优化的单克隆抗体。本发明的抗CD20抗体具有糖基化模式，其导致抗体组合物主要具有G0糖型，并且因此包含缺少岩藻糖（即非岩藻糖基化）的N-聚糖和与其连接的半乳糖残基。在一些实施方案中，这些抗CD20抗体包含利妥昔单抗抗CD20抗体的轻链和重链序列，因此代表非岩藻糖基化的利妥昔单抗。还提供了生产这些聚糖优化的抗CD20抗体的方法。

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