专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

141. 发明授权

US06929928B2 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US06929928B2
公开(公告)日：2005-08-16
申请号：US10808807
申请日：2004-03-24
申请人： Qiong Cheng , Natalia Sedkova , Luan Tao
发明人： Qiong Cheng , Natalia Sedkova , Luan Tao
IPC分类号： C07H21/04 , C12N20060101 , C12N1/14 , C12N1/20 , C12N1/21 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/90 , C12N15/52 , C12N15/82 , C12P5/00 , C12P5/02 , C12P7/26 , C12P23/00
CPC分类号： C12N9/00 , C12N9/0004 , C12N9/001 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/825 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：已经从成团Panthena菌株DC404中分离出独特的致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crtY-crtI-crtB-crtZ。包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ）。基因簇，基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

142. 发明申请

US20050164370A1 Transformed cell co-expressing cytokinin receptor and cytokinin biosynthesis enzyme 审中-公开
标题翻译：转化细胞共表达细胞分裂素受体和细胞分裂素生物合成酶
公开(公告)号：US20050164370A1
公开(公告)日：2005-07-28
申请号：US10960929
申请日：2004-10-12
申请人： Tatsuo Kakimoto , Masayuki Higuchi
发明人： Tatsuo Kakimoto , Masayuki Higuchi
IPC分类号： C07K14/415 , C12N9/10 , C12N1/18
CPC分类号： C12N9/1085 , C07K14/415
摘要： The present invention provides a transformed cell co-expressing a cytokinin receptor and a cytokinin biosynthesis enzyme and a method for analyzing the activity of controlling biosynthesis of cytokinins using such a transformed cell.
摘要翻译：本发明提供共表达细胞分裂素受体和细胞分裂素生物合成酶的转化细胞和使用这种转化细胞分析控制细胞分裂素生物合成的活性的方法。

143. 发明授权

US06855868B2 Geranylgeranyl pyrophosphate synthases 失效
公开(公告)号：US06855868B2
公开(公告)日：2005-02-15
申请号：US10108915
申请日：2002-03-28
申请人： Rebecca E. Cahoon , Mark E. Williams
发明人： Rebecca E. Cahoon , Mark E. Williams
IPC分类号： C12N9/10 , C12N15/82 , A01H1/00 , C07H21/04 , C07K14/415 , C12N5/14 , C12N9/00
CPC分类号： C12N9/1085 , C12N15/825 , C12N2799/021 , C12Q1/6895 , C12Y205/01029
摘要： This invention relates to an isolated nucleic acid fragment encoding a geranylgeranyl pyrophosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the geranylgeranyl pyrophosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the geranylgeranyl pyrophosphate synthase in a transformed host cell.

144. 发明申请

US20050003434A1 Isolated human enzyme proteins, nucleic acid molecules encoding human enzyme proteins, and uses thereof 审中-公开
公开(公告)号：US20050003434A1
公开(公告)日：2005-01-06
申请号：US10900420
申请日：2004-07-28
申请人： Ellen Beasley , Gennady Merkulov , Valentina Francesco
发明人： Ellen Beasley , Gennady Merkulov , Valentina Francesco
IPC分类号： C12N9/10 , C12Q1/68 , C07H21/04 , C12N9/00
CPC分类号： C12N9/1085
摘要： The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the enzyme peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the enzyme peptides, and methods of identifying modulators of the enzyme peptides.

145. 发明申请

US20040268439A1 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US20040268439A1
公开(公告)日：2004-12-30
申请号：US10808979
申请日：2004-03-25
发明人： Qiong Cheng , Luan Tao
IPC分类号： A01H001/00 , C12N015/82 , C12P023/00 , C07H021/04 , C12N009/10 , C12N005/04
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00
摘要： Genes have been isolated from strain DC260, a member of the Enterobacteriaceae family, encoding geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), null-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：基因已经从肠杆菌科家族的成员DC260中分离出来，编码香叶基香叶基焦磷酸（GGPP）合成酶（CrtE），八氢番茄红素合酶（CrtB），八氢番茄红素去饱和酶（CrtI），番茄红素环化酶（CrtY），β-胡萝卜素羟化酶）和玉米黄质葡萄糖基转移酶（CrtX）活性。这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

146. 发明申请

US20040268436A1 Method for production of C30-aldehyde carotenoids 失效
标题翻译：生产C30-醛类胡萝卜素的方法
公开(公告)号：US20040268436A1
公开(公告)日：2004-12-30
申请号：US10860291
申请日：2004-06-03
发明人： Qiong Cheng , Luan Tao
IPC分类号： C12N015/82 , C12N015/87 , C12N015/74 , A01H005/00
CPC分类号： C12N9/0083 , C12N9/1085 , C12N15/52 , C12P23/00
摘要： The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus aureus and Methylomonas sp. 16a can be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain of Escherichia coli that has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.
摘要翻译：本发明提供了用于生产C30-醛类胡萝卜素的微生物工程的方法。具体来说，来自金黄色葡萄球菌和Methylomonas sp。的crtM，sqs，crtN和crtN2基因的各种组合。 16a可以在转化体宿主中共表达，导致产生二头孢噻吩一醛，二卡非罗芬单醛和/或二卡必康二醛。在优选的实施方案中，将经遗传工程化的途径引入已被工程化用于表达类胡萝卜素的大肠杆菌菌株中，C30-类胡萝卜素产物是二卡必康二醛。

147. 发明申请

US20040253663A1 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US20040253663A1
公开(公告)日：2004-12-16
申请号：US10808807
申请日：2004-03-24
发明人： Qiong Cheng , Natalia Sedkova , Luan Tao
IPC分类号： C07H021/04 , C12P007/26 , C12P023/00 , C12N009/10 , C12N001/21
CPC分类号： C12N9/00 , C12N9/0004 , C12N9/001 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/825 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and null-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：已经从成团Panthena菌株DC404中分离出独特的致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crtY-crtI-crtB-crtZ。包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ）。基因簇，基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

148. 发明授权

US06821781B1 Method for selecting transformed plant cells using ethionine and cystathionine gamma synthase as the selection agent and marker gene 失效
标题翻译：使用乙硫氨酸和胱硫醚γ合酶作为选择剂和标记基因选择转化的植物细胞的方法
公开(公告)号：US06821781B1
公开(公告)日：2004-11-23
申请号：US09936454
申请日：2001-09-13
申请人： Nilgun E. Tumer , Thomas Leustek
发明人： Nilgun E. Tumer , Thomas Leustek
IPC分类号： C12N504
CPC分类号： C12N9/1085 , C12N9/88 , C12N15/8209 , C12N15/821 , C12N15/8243 , C12N15/8253
摘要： Disclosed are transgenic plants having edible portions that produce methional during processing. The plants contain increased methionine levels such that upon processing of the edible portion(s), methional levels are increased and lead to food products that possess improved flavor stability and/or quality. Plants of the Solanaceous family e.g., potato, tomato and eggplant, and other methional-producing plants including maize and soybean, are preferred plants. Several ways of genetically engineering plants to produce increased free Met levels are disclosed, with introduction of a non-native nucleic acid encoding cystathionine gamma synthase (CGS) and tissue-specific expression of an anti-sense S-adenosyl-methionine synthetase being preferred. Also disclosed are methods for selecting transformed plant cells using ethionine and CGS as the selection agent and marker gene respectively.
摘要翻译：公开了具有在处理过程中产生化学物质的可食用部分的转基因植物。植物含有增加的甲硫氨酸水平，使得在加工可食用部分时，甲基水平增加并导致具有改善的风味稳定性和/或质量的食品。茄科家族的植物，例如马铃薯，番茄和茄子，以及其它包括玉米和大豆在内的产甲植物是优选的植物。通过引入编码胱硫醚γ合成酶（CGS）的非天然核酸和反义S-腺苷 - 甲硫氨酸合成酶的组织特异性表达，公开了遗传工程植物产生增加的游离Met水平的几种方式。还公开了分别使用乙硫氨酸和CGS作为选择剂和标记基因选择转化的植物细胞的方法。

149. 发明授权

US06790633B1 Method of inhibiting a farnesyl transferase enzyme 失效
标题翻译：抑制法呢基转移酶的方法
公开(公告)号：US06790633B1
公开(公告)日：2004-09-14
申请号：US09665637
申请日：2000-09-19
申请人： Michael S. Brown , Joseph L. Goldstein , Yuval Reiss
发明人： Michael S. Brown , Joseph L. Goldstein , Yuval Reiss
IPC分类号： C12Q148
CPC分类号： C07K1/1077 , A61K38/00 , C07K5/1013 , C07K5/1016 , C07K5/1019 , C07K5/1024 , C07K7/02 , C07K7/06 , C07K14/82 , C12N9/1085
摘要： Disclosed are methods and compositions for the identification, characterization and inhibition of farnesyl protein transferases, enzymes involved in the farnesylation of various cellular proteins, including cancer related ras proteins such as p21ras. One farnesyl protein transferase which is disclosed herein exhibits a molecular weight of between about 70,000 and about 100,000 upon gel exclusion chromatography. The enzyme appears to comprise one or two subunits of approximately 50 kDa each. Methods are disclosed for assay and purification of the enzyme, as well as procedures for using the purified enzyme in screening protocols for the identification of possible anticancer agents which inhibit the enzyme and thereby prevent expression of proteins such as p21ras. Also disclosed is a families of compounds which act either as false substrates for the enzyme or as pure inhibitors and can therefore be employed for inhibition of the enzyme. The most potent inhibitors are ones in which phenylalanine occurs at the third position of a tetrapeptide whose amino terminus is cysteine.
摘要翻译：公开了用于鉴定，表征和抑制法呢基蛋白转移酶的方法和组合物，涉及各种细胞蛋白的法呢基化的酶，包括癌相关的ras蛋白如p21 。本文公开的一种法呢基蛋白转移酶在凝胶排阻色谱上显示约70,000至约100,000的分子量。该酶似乎包含一个或两个约50kDa的亚单位。公开了用于酶的测定和纯化的方法，以及在筛选方案中使用纯化酶鉴定可能的抗癌剂的方法，所述抗癌剂抑制酶，从而阻止蛋白质如p21 的表达。还公开了作为酶的假底物或纯抑制剂的化合物家族，因此可用于抑制酶。最有效的抑制剂是其氨基末端是半胱氨酸的四肽的第三位发生苯丙氨酸的抑制剂。

150. 发明申请

US20040161819A1 Isoprenoid synthases 有权
标题翻译：异戊二烯合酶
公开(公告)号：US20040161819A1
公开(公告)日：2004-08-19
申请号：US10635223
申请日：2003-08-05
申请人： PLANT RESEARCH INTERNATIONAL B.V.
发明人： Asaph Aharoni , Maarten Anthonie Jongsma , Henricus Andrianus Verhoeven , Hendrik Jan Bouwmeester
IPC分类号： C07H021/04 , C12N009/10
CPC分类号： C12N15/8282 , C12N9/1085 , C12N15/8243 , C12N15/8286 , Y02A40/162
摘要： The invention relates to the field of genetic engineering of flavor, fragrance and bio-control agent development. More specifically it relates to a process for production of natural flavors, fragrances or bio-control agents by the control of one or more genes implicated in that process. The invention provides an isolated or recombinant nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of flavor, fragrance and/or bio-control agent synthesis when provided with a suitable substrate under appropriate reaction conditions. The invention further provides a nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of synthesizing at least a monoterpene alcohol linalool when contacted with geranyl diphosphate (GPP) and/or at least a sesquiterpene alcohol nerolidol when contacted with farnesyl diphosphate (FPP) under appropriate reaction conditions.
摘要翻译：本发明涉及风味，香精和生物防治剂开发基因工程领域。更具体地说，本发明涉及通过控制与该方法相关的一种或多种基因来生产天然香料，香料或生物控制剂的方法。本发明提供了一种分离或重组的核酸或其功能片段，其在适当的反应条件下提供合适的底物时，基本上能够进行风味，香料和/或生物控制剂合成的蛋白质分子。本发明进一步提供编码蛋白质分子的核酸或功能片段，当与二磷酸法呢酯（FPP）接触时，当与二香草素二磷酸酯（GPP）和/或至少一种倍半萜烯醇橙花叔醇接触时，该蛋白质分子基本上能够合成至少一种单萜醇里哪醇在适当的反应条件下。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式