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131. 发明申请

US20050100533A1 Structures useful for bone engineering and methods 审中-公开
标题翻译：骨骼工程和方法有用的结构
公开(公告)号：US20050100533A1
公开(公告)日：2005-05-12
申请号：US10966930
申请日：2004-10-15
申请人： Rajendra Bhatnagar , Jing Qian
发明人： Rajendra Bhatnagar , Jing Qian
IPC分类号： A61K35/12 , A61K38/00 , A61L27/22 , A61L27/34 , A61L27/38 , C07K14/78 , C12N5/00 , C12N5/077 , A61F2/02 , C12N5/08 , A61K48/00
CPC分类号： A61L27/3804 , A61K35/12 , A61K38/00 , A61L27/22 , A61L27/34 , A61L27/3847 , A61L27/3895 , A61L2430/02 , C07K14/78 , C12N5/0068 , C12N5/0654 , C12N2506/1307 , C12N2533/18 , C12N2533/30 , C12N2533/50 , C12N2533/54 , C08L89/06
摘要： A bone repair apparatus is provided where a biologically compatible structure has a compound carried on the structure that mimics collagen binding to cells. Living cells derived from fibroblasts are carried on the structure and display at least one morphologic change consistent with an osteogenic phenotype. The preferred method for practicing the invention includes harvesting a quantity of fibroblasts from a patient in need of a bone graft, growing the tissue under cell growth conditions, and seeding at least some cells of the cultured tissue on the biologically compatible structure with the collagen mimic thereon. The culture tissue cells are seeded on the structure and incubated under cell growth conditions, which results in the differentiation of the cells to bone-like cells and thus provides a tissue engineered apparatus ready for use as a bone graft.
摘要翻译：提供骨修复装置，其中生物相容性结构具有携带在结构上的化合物，其模拟胶原与细胞的结合。从成纤维细胞衍生的活细胞携带在结构上并显示与成骨表型一致的至少一种形态学变化。用于实施本发明的优选方法包括从需要骨移植的患者中收获一定量的成纤维细胞，在细胞生长条件下生长组织，以及将所述培养组织的至少一些细胞与所述胶原模拟物上。将培养组织细胞接种在结构上并在细胞生长条件下孵育，这导致细胞分化成骨样细胞，从而提供准备用作骨移植物的组织工程设备。

132. 发明申请

US20040023374A1 Method and apparatus for multi-layer growth of anchorage dependent cells 审中-公开
标题翻译：锚固依赖细胞多层生长的方法和装置
公开(公告)号：US20040023374A1
公开(公告)日：2004-02-05
申请号：US10416561
申请日：2003-05-12
发明人： Catherine Rappaport , Edward Trujillo , Yvonne Rensch , Masoud Abbasi , Michael Kempe , Christian Rocaboy , John Gladysz
IPC分类号： C12N005/06 , C12M001/14 , C12M003/04
CPC分类号： C12N5/0068 , C12M23/08 , C12M23/20 , C12M23/24 , C12M23/38 , C12M23/48 , C12M29/04 , C12N2533/50
摘要： Anchorage-dependent cells are grown in a novel cell culture plate and on a novel substratum which increase the oxygenation of the cells. The cell culture plate is made by enclosing a growth chamber within a shell made of a solid sterilizable. One or more culture wells are positioned within the chamber. An inlet port and outlet port are fashioned within the shell for gas exchange. The wells have a well wall which allows for the diffusion of oxygen from the chamber into the well. A perfluorocarbon is placed within the well. A perfluoro-aldehyde is mixed with the perfluorocarbon, and the perfluoro-aldehyde re-orients so that the aldehyde head groups are at the interface. An attachment factor is bound to the perfluoro-aldehyde, which is sunk into the PFC substratum. Aqueous growth media is then added to the well, and anchorage-dependent cells added and allowed to grow.
摘要翻译：锚定依赖性细胞在新型细胞培养板和增加细胞氧合作用的新颖基质上生长。细胞培养板通过将生长室包封在由固体灭菌的壳体内制成。一个或多个培养孔位于室内。入口端口和出口端口形成在壳体内用于气体交换。井具有井壁，其允许氧从室扩散到井中。将全氟化碳置于该孔内。全氟醛与全氟化碳混合，全氟醛重新定向，使醛头基团在界面处。附着因子与全氟醛结合，其被沉积到PFC底物中。然后将水生长培养基加入到孔中，并且添加锚定依赖细胞并允许其生长。

133. 发明申请

US20030100108A1 Matrix for the production of tissue engineered ligaments, tendons and other tissue 有权
标题翻译：用于生产组织工程化韧带，腱和其他组织的基质
公开(公告)号：US20030100108A1
公开(公告)日：2003-05-29
申请号：US10008924
申请日：2001-11-16
发明人： Gregory H. Altman , David L. Kaplan , Rebecca L. Horan
IPC分类号： A61K035/64 , C12N005/00
CPC分类号： A61K9/70 , A61F2/00 , A61F2/08 , A61K35/12 , A61K35/28 , A61K38/17 , A61L27/227 , A61L27/3608 , A61L27/3662 , A61L27/3804 , A61L27/3821 , A61L27/3834 , A61L27/386 , A61L27/3895 , A61L27/48 , A61L2430/10 , C07K14/43518 , C12N5/066 , C12N5/0663 , C12N2533/50 , Y10T428/249921 , Y10T428/298 , Y10T442/2566 , C08L89/00
摘要： The present invention provides a novel silk-fiber-based matrix having a wire-rope geometry for use in producing a ligament or tendon, particularly an anterior cruciate ligament, ex vivo for implantation into a recipient in need thereof. The invention further provides the novel silk-fiber-based matrix which is seeded with pluripotent cells that proliferate and differentiate on the matrix to form a ligament or tendon ex vivo. Also disclosed is a bioengineered ligament comprising the silk-fiber-based matrix seeded with pluripotent cells that proliferate and differentiate on the matrix to form the ligament or tendon. A method for producing a ligament or tendon ex vivo comprising the novel silk-fiber-based matrix is also disclosed.
摘要翻译：本发明提供了一种新颖的基于丝纤维的基质，其具有用于产生韧带或腱，特别是前交叉韧带的钢丝绳几何形状，用于植入有需要的受体中。本发明进一步提供了新颖的丝纤维基质，该基质用多能细胞接种，其在基质上增殖和分化以离体形成韧带或肌腱。还公开了一种生物工程化韧带，其包含用多能细胞接种的基于丝纤维的基质，其在基质上增殖和分化以形成韧带或腱。还公开了一种用于生产包含新型丝纤维基质的离体的韧带或肌腱的方法。

134. 发明申请

US20030077825A1 Structures useful for bone engineering and methods 审中-公开
标题翻译：骨骼工程和方法有用的结构
公开(公告)号：US20030077825A1
公开(公告)日：2003-04-24
申请号：US10133289
申请日：2002-04-25
发明人： Rajendra S. Bhatnagar , Jing Jing Qian
IPC分类号： A61F002/28
CPC分类号： A61L27/3804 , A61K35/12 , A61K38/00 , A61L27/22 , A61L27/34 , A61L27/3847 , A61L27/3895 , A61L2430/02 , C07K14/78 , C12N5/0068 , C12N5/0654 , C12N2506/1307 , C12N2533/18 , C12N2533/30 , C12N2533/50 , C12N2533/54 , C08L89/06
摘要： A bone repair apparatus is provided where a biologically compatible structure has a compound carried on the structure that mimics collagen binding to cells. Living cells derived from fibroblasts are carried on the structure and display at least one morphologic change consistent with an osteogenic phenotype. The preferred method for practicing the invention includes harvesting a quantity of fibroblasts from a patient in need of a bone graft, growing the tissue under cell growth conditions, and seeding at least some cells of the cultured tissue on the biologically compatible structure with the collagen mimic thereon. The culture tissue cells are seeded on the structure and incubated under cell growth conditions, which results in the differentiation of the cells to bone-like cells and thus provides a tissue engineered apparatus ready for use as a bone graft.
摘要翻译：提供骨修复装置，其中生物相容性结构具有携带在结构上的化合物，其模拟胶原与细胞的结合。从成纤维细胞衍生的活细胞携带在结构上并显示与成骨表型一致的至少一种形态学变化。用于实施本发明的优选方法包括从需要骨移植的患者中收获一定量的成纤维细胞，在细胞生长条件下生长组织，以及将所述培养组织的至少一些细胞与所述胶原模拟物上。将培养组织细胞接种在结构上并在细胞生长条件下孵育，这导致细胞分化成骨样细胞，从而提供准备用作骨移植物的组织工程设备。

135. 发明授权

US06440740B1 Material for activating epidermal cell multiplication 失效
标题翻译：激活表皮细胞增殖的材料
公开(公告)号：US06440740B1
公开(公告)日：2002-08-27
申请号：US09341973
申请日：1999-07-16
申请人： Kozo Tsubouchi , Hiromi Yamada , Yoko Takasu
发明人： Kozo Tsubouchi , Hiromi Yamada , Yoko Takasu
IPC分类号： C12N500
CPC分类号： A61L15/32 , C12N5/0068 , C12N5/0629 , C12N2533/50
摘要： The present invention provides a novel material for activating epidermal cell multiplication on which not only effective adhesion and multiplication of human and mammalian epidermal cells is accomplished also intercellular adhesion is promoted to form a plain cellular layer. The present invention provides a material for activating epidermal cell multiplication which contains silk fibroin of &agr;-type or &agr;-helix X-ray diffraction pattern as a principal constituent. The present material fulfills the principal requirements for activating epidermal cells of greatly improved cellular adhesion rate, multiplication, intercellular adhesion and growth of cell layers.
摘要翻译：本发明提供了一种用于激活表皮细胞增殖的新型材料，其不仅实现人和哺乳动物表皮细胞的有效粘附和增殖，而且促进细胞间粘附以形成平坦的细胞层。本发明提供一种激活表皮细胞增殖的材料，其含有α型或α-螺旋X射线衍射图的丝素蛋白作为主要成分。本发明材料满足激活表皮细胞的主要要求，大大提高细胞粘附率，增殖，细胞间粘附和细胞层生长。

136. 发明申请

US20020094573A1 Cardiovascular components for transplantation and methods of making thereof 审中-公开
标题翻译：用于移植的心血管成分及其制备方法
公开(公告)号：US20020094573A1
公开(公告)日：2002-07-18
申请号：US10024880
申请日：2001-12-19
发明人： Eugene Bell
IPC分类号： A61F002/24
CPC分类号： A61L27/3691 , A61F2/2415 , A61K35/34 , A61K35/44 , A61L27/3604 , A61L27/362 , A61L27/3687 , A61L27/507 , A61L2430/40 , C12N5/0656 , C12N2502/02 , C12N2502/14 , C12N2533/50 , C12N2533/54
摘要： Cardiovascular components such as biocompatible heart valves and annular sewing rings are disclosed, as well as, methods for making the same. The heart valves include biodegradable polymer fiber scaffolds and collagen. Also disclosed are donor aortic heart valves processed without the use of crosslinking chemicals.
摘要翻译：公开了诸如生物相容性心脏瓣膜和环形缝合环之类的心血管成分，以及制造它们的方法。心脏瓣膜包括可生物降解的聚合物纤维支架和胶原蛋白。还公开了不使用交联化学品处理的供体主动脉瓣心脏瓣膜。

137. 发明申请

US20020076816A1 Stem cells and signals developed for use in tissue and organ repair and replacement 审中-公开
标题翻译：开发的干细胞和信号用于组织和器官修复和置换
公开(公告)号：US20020076816A1
公开(公告)日：2002-06-20
申请号：US10005053
申请日：2001-12-04
发明人： Jianwu Dai , Eugene Bell
IPC分类号： C12N005/08 , C12N015/87 , C12N005/06
CPC分类号： C12N5/0629 , A61K35/12 , C12N5/0656 , C12N2502/14 , C12N2506/00 , C12N2506/1307 , C12N2533/50 , C12N2533/54
摘要： Methods and compositions for repairing tissue. Certain embodiments of the invention involve transdifferentiation of cells in a manner not heretofore provided for. One embodiment of the invention features methods for producing stem cells. These methods can involve exposing cells (e.g., human fibroblasts) to a processed or activated egg extract (e.g., activated egg extract); and culturing the cells for a period of time to become stem cells. A cell culture can be performed in two or three dimensions, so that organ tissue or whole organs may be produced, e.g., for transplantation. Another embodiment of the invention features methods for promoting wound healing by using signaling complexes.
摘要翻译：用于修复组织的方法和组合物。本发明的某些实施方案涉及以前所未有的方式对细胞进行转分化。本发明的一个实施方案的特征在于制备干细胞的方法。这些方法可以包括将细胞（例如人成纤维细胞）暴露于加工或活化的卵提取物（例如活化的卵提取物）中; 并将细胞培养一段时间以成为干细胞。可以在两个或三个维度上进行细胞培养，使得可以产生器官组织或整个器官，例如用于移植。本发明的另一个实施方案的特征在于使用信号复合物促进伤口愈合的方法

138. 发明申请

US20020068051A1 Generation and use of signal-plexes to develop specific cell types, tissues and /or organs 审中-公开
标题翻译：信号丛的生成和使用来开发特定的细胞类型，组织和/或器官
公开(公告)号：US20020068051A1
公开(公告)日：2002-06-06
申请号：US09901765
申请日：2001-07-09
发明人： Jianwu Dai , Eugene Bell
IPC分类号： A61K048/00 , A61K035/00
CPC分类号： C12N5/0656 , A61K35/12 , C12N2502/14 , C12N2506/00 , C12N2533/50 , C12N2533/54
摘要： This invention includes methods and compositions for generating signaling complexes (nullSignal-plexesnull) and the use of Signal-plexes for inducing cell divisions, differentiation and transdifferentiation of cells into specific cell, tissue or organ types which resemble the cells, tissues or organs from which the Signal-plexes were derived. Signal-plexes may be used to induce the development of any specific cells, tissues or organs of the body. Signal-plexes may be derived from any tissue of the body. Signal-plexes may be combined with stem cells or cells having stem cell properties in scaffolds for differentiation into tissue or organs in vitro and or in vivo. The applications of this invention comprise tissue repair, and tissue or organ regeneration. Signal-plexes may be used as pharmaceutical agents; they may be used in humans as well as non-humans.
摘要翻译：本发明包括用于产生信号复合物（“Signal-plex”）的方法和组合物，以及使用信号聚合酶诱导细胞分裂，细胞分化和转分化为类似于细胞，组织或器官的特定细胞，组织或器官类型信号丛从中衍生出来。信号丛可用于诱导人体任何特定细胞，组织或器官的发育。信号丛可能来源于身体的任何组织。信号丛可以与干细胞或在支架中具有干细胞特性的细胞在体外和/或体内分化成组织或器官。本发明的应用包括组织修复和组织或器官再生。信号聚苯乙烯可以用作药剂; 它们可能用于人类以及非人类。

139. 发明授权

US06251672B1 Culturing mammalian cells in contact with cell surface proteins 失效
标题翻译：培养与细胞表面蛋白质接触的哺乳动物细胞
公开(公告)号：US06251672B1
公开(公告)日：2001-06-26
申请号：US09197470
申请日：1998-11-23
申请人： Manfred Kubbies , Peter Dörmer , Petra Meissner
发明人： Manfred Kubbies , Peter Dörmer , Petra Meissner
IPC分类号： C12N500
CPC分类号： C12N5/0068 , C12N2533/50
摘要： Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.
摘要翻译：需要与细胞表面蛋白质接触以激活，分化和/或增殖的哺乳动物细胞与来自哺乳动物细胞的固定的膜囊泡或来自至少两个哺乳动物细胞群体的固定的膜囊泡接触固定的哺乳动物细胞培养。哺乳动物细胞或膜囊泡可以用生物结合对的第一个配偶体修饰，并通过生物结合对的第二个配偶体固定在固体载体上。可以用第一固定化膜囊泡培养，随后用第二固定化膜囊泡培养。膜囊泡包含哺乳动物细胞的天然表面的一部分，其包括触发表面蛋白的信号。膜泡可以通过细胞表面分子的结合配偶体固定。

140. 发明授权

US6165496A Keratin-based sheet material for biomedical applications and method of production 有权
标题翻译：用于生物医学应用的角蛋白片材和生产方法
公开(公告)号：US6165496A
公开(公告)日：2000-12-26
申请号：US365976
申请日：1999-08-02
申请人： Scott F. Timmons , Cheryl R. Blanchard , Robert A. Smith
发明人： Scott F. Timmons , Cheryl R. Blanchard , Robert A. Smith
IPC分类号： A61K9/70 , A61K35/36 , A61K38/39 , A61L15/32 , A61L26/00 , A61L27/22 , A61L27/34 , A61L27/60 , A61L31/04 , A61L31/10 , C08H1/06 , C12N5/00 , A61K9/00 , A61K38/00 , A61F13/00 , A61L15/16
CPC分类号： C12N5/0068 , A61K9/70 , A61K35/36 , A61K38/39 , A61L15/32 , A61L26/0047 , A61L26/008 , A61L27/227 , A61L27/34 , A61L27/60 , A61L31/047 , A61L31/10 , C08H1/06 , C12N2533/50 , Y10S530/842 , C08L89/04 , C08L89/00
摘要： A sheet wound dressing formed of cross linked keratin. An insoluble, largely Beta keratin fraction from human hair is acidified to a low pH, preferably less than about 3, which partially solubilizes the keratin by weakening hydrogen bonds. The suspension is added to base, such as ammonium hydroxide, forming a slurry. The slurry is cast directly onto a flat surface, allowing the re-formation of cross-links including hydrogen bonds and disulfide bonds. The resulting cross-linked keratin sheet can be used as a sheet wound dressing or as a scaffolding for growth of cells. The insoluble keratin can be derived from human hair which is washed, rinsed, dried, chopped and treated with peracetic acid to break some accessible disulfide linkages. The treated hair is filtered, rinsed, dried, and ground into a keratin powder. The keratin powder is suspended in a mixture of ammonium hydroxide and ammonium thioglycollate and heated sufficiently to dissolve the soluble keratin fraction, followed by cooling and centrifugation to concentrate the insoluble, largely Beta keratin fraction.
摘要翻译：由交联角蛋白形成的伤口敷料。将来自人类头发的不溶性大部分β角蛋白级分酸化至低pH，优选小于约3，通过削弱氢键来部分溶解角蛋白。将悬浮液加入到碱如氢氧化铵中，形成浆料。将浆料直接浇铸到平坦表面上，允许重新形成包括氢键和二硫键的交联。所得到的交联角蛋白片可用作片状伤口敷料或用作细胞生长的支架。不溶性角蛋白可以衍生自人毛，洗涤，漂洗，干燥，切碎并用过乙酸处理以破坏一些可接近的二硫键。将经处理的毛发过滤，漂洗，干燥并研磨成角蛋白粉末。将角蛋白粉末悬浮在氢氧化铵和巯基乙酸铵的混合物中并充分加热以溶解可溶性角蛋白级分，然后冷却并离心以浓缩不溶性的大部分β角蛋白级分。

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