专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

131. 发明授权

US06485930B1 Mannosyl transfer with regeneration of GDP-mannose 失效
标题翻译：甘露糖转移与GDP-甘露糖再生
公开(公告)号：US06485930B1
公开(公告)日：2002-11-26
申请号：US08122229
申请日：1993-09-15
申请人： Chi-Huey Wong
发明人： Chi-Huey Wong
IPC分类号： C12P100
CPC分类号： C12N9/1051 , C12P19/18 , C12P19/32 , C12P21/005
摘要： A one-pot glycosylation reaction is disclosed in which a mannosyl (Man) group is enzymatically transferred to an acceptor molecule. The starting glycoside is a mannosyl 1-phosphate that is enzymatically converted to its GDP derivative via UTP and a pyrophorylase. The formed GDP derivative is used in the enzyme-catalyzed glycosyl transfer. That enzyme-catalyzed glycosyl transfer to an acceptor releases GDP that is enzymatically converted to GTP for further conversion of mannosyl 1-phosphate into its GDP derivative. Also disclosed are a recombinant &agr;1,2-mannosyltransferase that is enzymatically active, is dispersible in an aqueous reaction medium, and free of the transmembrane portion of the native enzyme, as well as DNA encoding that transferase, an expression vector containing exogenous DNA that encodes that enzyme and E. coli cells containing that vector.
摘要翻译：公开了一锅糖基化反应，其中甘露糖基（Man）基团被酶转移到受体分子。起始糖苷是通过UTP和自发磷酸酶酶转化成其GDP衍生物的甘露糖基1-磷酸酯。形成的GDP衍生物用于酶催化的糖基转移。酶催化的糖基转移到受体释放GDP，其被酶转化为GTP以进一步将1-磷酸甘露糖基转化成其GDP衍生物。还公开了一种重组α1,2-甘露糖基转移酶，其是酶促活性的，可分散在水性反应介质中，并且不含天然酶的跨膜部分，以及编码该转移酶的DNA，含有外源DNA的表达载体，其编码该酶和含有该载体的大肠杆菌细胞。

132. 发明授权

US06168934A Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
标题翻译：寡糖酶底物和抑制剂：方法和组合物
公开(公告)号：US06168934A
公开(公告)日：2001-01-02
申请号：US09072958
申请日：1998-05-05
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C12P1918
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
摘要翻译：公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。还公开了糖基化的方法。用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

133. 发明授权

US5981267A Enantioselection of amines using homocarbonates with hydrolase 失效
标题翻译：使用碳氢化合物与水解酶对映体选择胺
公开(公告)号：US5981267A
公开(公告)日：1999-11-09
申请号：US590530
申请日：1996-01-24
申请人： Chi-Huey Wong , Bernard Orsat , Wilna J. Moree , Shu Takayama
发明人： Chi-Huey Wong , Bernard Orsat , Wilna J. Moree , Shu Takayama
IPC分类号： C12P13/00 , C07C1/04
CPC分类号： C12P13/001 , C12P13/02
摘要： Racemic amines are enzymically converted by enantioselective carbamation to produce to chiral carbamates. Simple homocarbonates employed as substrates. The chiral carbamates may then be deprotected to yield the original amine in a chiral form. Alternatively, the chiral carbamates may be reduced so as to produce methylated chiral amines.
摘要翻译：外消旋胺通过对映选择性氨基甲酸酯化反应生成手性氨基甲酸酯。简单的碳酸氢盐用作底物。然后将手性氨基甲酸酯脱保护，得到手性形式的原始胺。或者，可以还原手性氨基甲酸酯以产生甲基化手性胺。

134. 发明授权

US5514784A Thio linked glycosyl compounds 失效
标题翻译：硫醇连接糖基化合物
公开(公告)号：US5514784A
公开(公告)日：1996-05-07
申请号：US36334
申请日：1993-03-19
申请人： Chi-Huey Wong , Hirosato Kondo
发明人： Chi-Huey Wong , Hirosato Kondo
IPC分类号： C07H15/203 , C07G3/00 , C07G11/00 , C07H15/00 , C07H17/00
CPC分类号： C07H15/203
摘要： Thio linked glycosyl compounds are disclosed and synthesized. The synthesis employs a blocked carbohydrate donor and a blocked glycosyl acceptor. The carbohydrate donor includes an acid labile phosphite leaving group attached to the anomeric carbon. The blocked glycosyl acceptor includes an unprotected thio group susceptible to electrophilic attack. The reaction is initiated by the addition of a Lewis acid so as to activate the acid labile phosphite leaving group on the carbohydrate donor. The substitution reaction may be conducted at -78.degree. C. in a solvent such as Et.sub.3 N which favors the formation of thio linked glycosylation products.
摘要翻译：公开并合成了Thio连接的糖基化合物。该合成使用封闭的碳水化合物供体和封闭的糖基受体。碳水化合物供体包括与异头碳连接的离去基团的酸不稳定的亚磷酸酯。封闭的糖基受体包括易受亲电发作攻击的未受保护的硫代基团。通过加入路易斯酸来引发反应，以活化碳水化合物供体上的酸不稳定亚磷酸酯离去基团。取代反应可以在-78℃下在诸如Et 3 N的溶剂中进行，这有利于硫代连接的糖基化产物的形成。

135. 发明授权

US5329025A 3-azido compound 失效
标题翻译： 3-叠氮基化合物
公开(公告)号：US5329025A
公开(公告)日：1994-07-12
申请号：US862157
申请日：1992-04-02
申请人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
发明人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
IPC分类号： C07C45/89 , C07C247/04 , C07C247/02 , C07C205/05 , C07C207/00 , C07C247/00
CPC分类号： C07C247/04 , C07C45/89
摘要： A new and practical method for synthesizing heterocyclic polyhydroxylated alkaloids using enzymatic aldol condensation and catalytic intramolecular reductive amination is disclosed.
摘要翻译：公开了一种使用酶促醛缩醇缩合和催化分子内还原胺化合成杂环多羟基化生物碱的新型实用方法。

136. 发明授权

US5229523A 2-methyl-5-hydroxymethyl- and 2,5-dimethyl-3,4-dihydroxypyrrolidines 失效
标题翻译： 2-甲基-5-羟基甲基 - 和2,5-二甲基-3,4-二羟基吡咯烷
公开(公告)号：US5229523A
公开(公告)日：1993-07-20
申请号：US835238
申请日：1992-02-13
申请人： Chi-Huey Wong , Kun-Chin Liu
发明人： Chi-Huey Wong , Kun-Chin Liu
IPC分类号： C07D207/12 , C07D207/46 , C07H7/02 , C07H11/00
CPC分类号： C07D207/46 , C07D207/12 , C07H11/00 , C07H7/02
摘要： 2-Methyl-5-hydroxymethyl- and 2,5-dimethyl-3,4-dihydroxypyrrolidines, 2,5-dimethyl-3,4-dihydroxypyrrolidines, their processes of preparation and use are disclosed. 5-Azido-5-deoxyhexulose-1-phosphate compounds and processes of making the same are also disclosed.
摘要翻译：公开了2-甲基-5-羟甲基 - 和2,5-二甲基-3,4-二羟基吡咯烷酮，2,5-二甲基-3,4-二羟基吡咯烷的制备方法和用途。还公开了5-叠氮基-5-脱氧己酮糖-1-磷酸酯化合物及其制备方法。

137. 发明授权

US5106750A Enantio- and regioselective synthesis of organic compounds using enol esters as irreversible transacylation reagents 失效
标题翻译：有机化合物的有机合成和有机合成作为不可逆的交联试剂
公开(公告)号：US5106750A
公开(公告)日：1992-04-21
申请号：US396723
申请日：1989-08-24
申请人： Chi-Huey Wong , Yi-Fong Wang , William J. Hennen , Kevin A. Babiak , John H. Dygos , John S. Ng
发明人： Chi-Huey Wong , Yi-Fong Wang , William J. Hennen , Kevin A. Babiak , John H. Dygos , John S. Ng
IPC分类号： C12P7/62 , C12P9/00 , C12P13/00 , C12P17/02 , C12P19/02 , C12P19/44 , C12P41/00
CPC分类号： C12P41/004 , C12P17/02 , C12P19/02 , C12P19/44 , C12P7/62 , C12P9/00
摘要： A process for irreversible regio- and stereoselective enzyme catalyzed acylation of alcohols using enol esters as acylating reagents is disclosed. The present invention permits the selective modification of hydroxyl group(s) of chiral and meso alcohols, including sugars, organometallics, and glycosides. The enol freed upon transesterification rapidly tautomerizes to the corresponding volatile aldehyde or ketone thereby preventing the reverse reaction from occurring.

138. 发明授权

US5075225A Process for the enzymatic synthesis of nucleosides 失效
标题翻译：核苷酸酶的合成方法
公开(公告)号：US5075225A
公开(公告)日：1991-12-24
申请号：US334699
申请日：1989-04-06
申请人： Chi-Huey Wong , William J. Hennen
发明人： Chi-Huey Wong , William J. Hennen
IPC分类号： C12P19/38
CPC分类号： C12P19/38
摘要： A process for synthesizing nucleosides is disclosed. The process includes the reaction of an alkylated nucleoside (such as 7-methylguanosine or 7-methylinosine) with a heterocyclic base (such as adenine, 3-deazaadenine or 1,2,4-triazole-3-carboxamide) in the presence of a nucleoside-forming enzyme to form a nucleoside that includes a glycosyl component, donate by the alkylated nucleoside, bonded to the heterocyclic base.

139. 发明授权

US10338069B2 Glycan arrays for high throughput screening of viruses 有权
公开(公告)号：US10338069B2
公开(公告)日：2019-07-02
申请号：US14376837
申请日：2011-04-12
申请人： Chi-Huey Wong , Chung-Yi Wu , Chi-Hui Liang , An-Suei Yang
发明人： Chi-Huey Wong , Chung-Yi Wu , Chi-Hui Liang , An-Suei Yang
IPC分类号： G01N33/569 , G01N33/53 , G01N33/543
摘要： Glycan arrays that can detect and distinguish between various sub-types and strains of influenza virus are provided. Methods for using the glycan arrays with assays using nanoparticle amplification technique are disclosed. Sandwich assays using gold nanoparticles conjugated to phage particles comprising influenza virus-specific antibodies for detecting multiple serotypes using a single reaction are provided. Plurality of glycans directed to specific target HA of influenza virus comprises the array. Detector molecules comprising noble metals conjugated to (a) phage display particles expressing antibodies against hemagglutinin and (b) neuraminidase binding agents are disclosed.

140. 发明授权

US10307470B2 Antibody-mediated anti-tumor activity induced by Reishi mushroom polysaccharides 有权
公开(公告)号：US10307470B2
公开(公告)日：2019-06-04
申请号：US14906663
申请日：2014-07-26
申请人： ACADEMIA SINICA , Chi-Huey Wong , Chung-Yi Wu , Hsien-Yeh Hsu , Shih-Fen Liao , Chi-Hui Liang
发明人： Chi-Huey Wong , Chung-Yi Wu , Hsien-Yeh Hsu , Shih-Fen Liao , Chi-Hui Liang
IPC分类号： A61K39/00 , A61K36/074
摘要： Immunogenic compositions, cancer vaccines and methods for treating cancer comprising FMS, the fucose-enriched polysaccharide fraction from Reishi F3, are provided. Compositions comprise fucose-enriched Reishi polysaccharide fraction (FMS) MW=˜35 kDa, wherein the FMS is isolated by size-exclusion chromatography from Reishi F3, and the FMS comprises polysaccharides having primarily a backbone selected from 1,4-mannan and 1,6-α-galactan, wherein the backbone is linked to a terminal fucose-containing side-chain Immunogenic compositions comprising glycolipid adjuvants are provided. Antibodies generated by immunogenic compositions disclosed herein bind cancer cells comprising antigens Globo H, Globo H, Gb3, Gb4, Gb5 (SSEA-3) and SSEA-4 on the cell surface.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式