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    • 123. 发明授权
    • Cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same
    • 衍生自酵母的细胞壁锚蛋白,其基因和使用其的细胞表面表达系统
    • US07132273B1
    • 2006-11-07
    • US10333951
    • 2000-07-27
    • Eui-Sung ChoiJung-Hoon SohnSo-Young Kim
    • Eui-Sung ChoiJung-Hoon SohnSo-Young Kim
    • C12N1/20C12N15/00C12N1/14C12P21/04C12Q1/00C07K1/00C07H21/04C12Q1/68
    • C07K14/39
    • The present invention provides novel cell wall anchor proteins derived from yeast, genes thereof and the genetic method for tethering polypeptide to the yeast cell wall using the same. Particularly, the present invention provides the novel GPI (glycosyl phosphatidyl inositol)-anchor protein genes, SFD1, GAS1, TIP1 and CWP1, and their proteins, PIR2 cell wall protein gene and its protein derived from Hansenula polymorpha, and the cell surface expression system using them which immobilize foreign enzymes or polypeptides on the cell wall of a microbial cell. In addition, the present invention provides the cell surface expression system using WSC1 gene and its protein derived from yeasts, including Hansenula polymorpha and Saccharomyces cerevisiae, and STA1 gene and its protein derived from Saccharomyces diastaticus. The cell surface expression system of the present invention expects an immobilization effect as biocatalysts by adhereing a desired protein to the cell surface, and provides a means of altering target protein characteristics such as binding affinity and stability by library screening.
    • 本发明提供了源自酵母的新型细胞壁锚蛋白,其基因,以及使用该酵母细胞壁将酵母细胞壁连接多肽的遗传方法。 特别地,本发明提供新型GPI(糖基磷脂酰肌醇) - 锚定蛋白基因,SFD1,GAS1,TIP1和CWP1及其蛋白质,PIR2细胞壁蛋白基因及其衍生自多形汉逊酵母的蛋白质,以及细胞表面表达系统 使用它们将外来酶或多肽固定在微生物细胞的细胞壁上。 此外,本发明提供了使用WSC1基因及其衍生自酵母的蛋白质,包括多形汉逊酵母和酿酒酵母的细胞表面表达系统,以及来源于糖酵母的STA1基因及其蛋白质。 本发明的细胞表面表达系统期望通过将期望的蛋白质粘附到细胞表面作为生物催化剂的固定效果,并且通过文库筛选提供改变靶蛋白特征如结合亲和力和稳定性的方法。
    • 125. 发明授权
    • Apparatus and method for selecting length of variable length coding bit stream using neural network
    • 使用神经网络选择可变长度编码比特流的长度的装置和方法
    • US06885320B2
    • 2005-04-26
    • US10760400
    • 2004-01-21
    • So-young Kim
    • So-young Kim
    • H04N7/24H03M7/40H04N7/26
    • H03M7/40H04N19/436H04N19/91
    • An apparatus and method for selecting the length of a variable length code bitstream by using a neural network are provided. The apparatus for selecting the length of a variable length code bitstream includes a bitstream estimation length receiving unit which inputs a predetermined quantization DCT coefficient block to a neural network whose training is finished, and receives the estimation length of a bitstream corresponding to the quantization DCT coefficient block from the neural network; and a bitstream estimation length selection unit which receives user selection about an estimation length received by the bitstream estimation length receiving unit. According to the method and apparatus the length of a variable length code bit stream can be estimated such that a user can select a desired length of a bitstream in advance without performing variable length coding.
    • 提供了一种通过使用神经网络来选择可变长度码位流的长度的装置和方法。 用于选择可变长度代码比特流长度的装置包括:比特流估计长度接收单元,其将预定的量化DCT系数块输入到训练完成的神经网络,并且接收与量化DCT系数对应的比特流的估计长度 阻塞神经网络; 以及比特流估计长度选择单元,其接收关于由比特流估计长度接收单元接收的估计长度的用户选择。 根据该方法和装置,可以估计可变长度码位流的长度,使得用户可以预先选择比特流的期望长度而不执行可变长度编码。