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111. 发明授权

US07713698B2 Massive parallel method for decoding DNA and RNA 有权
公开(公告)号：US07713698B2
公开(公告)日：2010-05-11
申请号：US11894690
申请日：2007-08-20
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C07H19/04 , C07G3/00 , C07H21/00
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

112. 发明申请

US20090298072A1 DNA Sequencing by Nanopore Using Modified Nucleotides 有权
标题翻译：使用修饰的核苷酸的纳米孔DNA测序
公开(公告)号：US20090298072A1
公开(公告)日：2009-12-03
申请号：US12308091
申请日：2007-06-07
申请人： Jingyue Ju
发明人： Jingyue Ju
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6869 , C12Q2565/631 , C12Q2525/101 , C12Q2565/101
摘要： This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides.
摘要翻译：本发明提供了通过使用纳米孔和修饰的核苷酸对单链DNA进行测序的方法。

113. 发明申请

US20080319179A1 Massive parallel method for decoding DNA and RNA 有权
公开(公告)号：US20080319179A1
公开(公告)日：2008-12-25
申请号：US11810509
申请日：2007-06-05
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C07H21/04
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

114. 发明授权

US07345159B2 Massive parallel method for decoding DNA and RNA 有权
公开(公告)号：US07345159B2
公开(公告)日：2008-03-18
申请号：US10702203
申请日：2003-11-06
申请人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
发明人： Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

115. 发明授权

US07074597B2 Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry 有权
标题翻译：使用固相可俘获双脱氧核苷酸和质谱的多重基因分型
公开(公告)号：US07074597B2
公开(公告)日：2006-07-11
申请号：US10194882
申请日：2002-07-12
申请人： Jingyue Ju
发明人： Jingyue Ju
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , C07H19/00 , C07H21/02 , C07H21/04 , C12Q1/6872 , C12Q2535/125 , C12Q2523/319 , C12Q2563/167 , C12Q2563/131
摘要： This invention provides methods for detecting single nucleotide polymorphisms and multiplex genotyping using dideoxynucleotides and mass spectrometry.
摘要翻译：本发明提供了使用双脱氧核苷酸和质谱法检测单核苷酸多态性和多重基因分型的方法。

116. 发明授权

US07015000B2 Probes labeled with energy transfer coupled dyes 失效
公开(公告)号：US07015000B2
公开(公告)日：2006-03-21
申请号：US10288104
申请日：2002-11-04
申请人： Richard Mathies , Alexander Glazer , Jingyue Ju
发明人： Richard Mathies , Alexander Glazer , Jingyue Ju
IPC分类号： C12Q1/68
CPC分类号： G01N33/582 , C12Q1/6818 , C12Q1/6869 , G01N33/542 , C12Q2537/143 , C12Q2565/101 , C12Q2535/101 , C12Q2563/107 , C12Q2565/1015
摘要： Fluorescent labels having at least one donor and at least one acceptor fluorophore bonded to a polymeric backbone in energy transfer relationship, as well as methods for their use, are provided. Of particular interest are the subject labels wherein the polymeric backbone is a nucleic acid and the donor fluorophore is bonded to the 5′ terminus of said nucleic acid. Such labels find use as primers in applications involving nucleic acid chain extension, such as sequencing, PCR and the like.

117. 发明授权

US5853992A Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels 失效
标题翻译：在能量转移标签中具有高吸光度横截面作为供体发色团的花青染料
公开(公告)号：US5853992A
公开(公告)日：1998-12-29
申请号：US726178
申请日：1996-10-04
申请人： Alexander N. Glazer , Richard A. Mathies , Su-Chun Hung , Jingyue Ju
发明人： Alexander N. Glazer , Richard A. Mathies , Su-Chun Hung , Jingyue Ju
IPC分类号： C09B23/00 , C09K11/06 , C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译：能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。

118. 发明授权

US5728528A Universal spacer/energy transfer dyes 失效
标题翻译：通用间隔/能量转移染料
公开(公告)号：US5728528A
公开(公告)日：1998-03-17
申请号：US531132
申请日：1995-09-20
申请人： Richard A. Mathies , Alexander N. Glazer , Jingyue Ju
发明人： Richard A. Mathies , Alexander N. Glazer , Jingyue Ju
IPC分类号： C12N15/09 , C07H21/00 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C07H21/00 , C12Q1/6818 , Y10S435/81
摘要： Fluorescent labels are provided employing energy absorber/donor components and energy acceptor/fluorescer components joined together by a spacer which comprises sugar phosphate monomeric units, particularly ribosyl phosphate monomeric units, where the sugar groups are free of active hydroxyl groups. Particularly, an energy transfer component is substituted at the 5' position of the spacer chain, while the other energy transfer component is substituted at the 1' position of the 3' terminal ribosyl group of the label forming an ET cassette for linking to a nucleic acid sequence with any compositions. By employing combinations of ET components, with a common energy absorber/donor and different fluorescers, one can provide for families of labels which can be tagged to any target molecules and which can be excited at a single wavelength and fluoresce at different wavelengths with large Stokes shifts. The compositions find particular application in sequencing.
摘要翻译：提供使用能量吸收剂/供体组分和能量受体/荧光剂组分的荧光标记，其通过间隔物连接在一起，所述间隔物包含糖磷酸酯单体单元，特别是磷酸核糖基单体单元，其中糖基团不含活性羟基。特别地，能量转移组分在间隔链的5'位被取代，而另一能量转移组分在形成用于连接到核酸的ET盒的标记的3'端核糖基的1'位置被取代酸序列与任何组合物。通过使用ET组分与共同的能量吸收剂/供体和不同的荧光剂的组合，可以提供可以标记任何靶分子的标记系列，并且可以在单个波长处被激发并且在具有大的Stokes的不同波长下发出荧光转变。该组合物在测序中发现具体应用。

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