专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

101. 发明授权

US06921818B2 Purification of oligomers 有权
标题翻译：低聚物的纯化
公开(公告)号：US06921818B2
公开(公告)日：2005-07-26
申请号：US10627934
申请日：2003-07-25
申请人： Brian S. Sproat
发明人： Brian S. Sproat
IPC分类号： C07H23/00 , C07F7/08 , C07F7/18 , C07F9/24 , C07H19/06 , C07H21/00 , C07H19/04 , C12Q1/68 , C01B33/08
CPC分类号： C07H19/06 , C07F7/1804 , C07H21/00
摘要： Compositions and methods are disclosed which facilitate purification of oligomers and other compounds. The disclosed compositions are silyl compositions that can be directly coupled, or coupled through a linking group, to a compound of interest, preferably to an oligomer at the end of oligomer synthesis. The silicon atom includes between one and three sidechains that function as capture tags. In one embodiment, the capture tags are lipophilic, which allows a derivatized oligomer to be separated from failure sequences by reverse phase chromatography. In another embodiment, the capture tags are compounds with a known affinity for other compounds, which other compounds are preferably associated with a solid support to allow chromatographic separation. Examples include haptens, antibodies, and ligands. Biotin, which can bind to or interact with a streptavidin-bound solid support, is a preferred capture tag of this type.
摘要翻译：公开了有助于纯化低聚物和其它化合物的组合物和方法。所公开的组合物是甲硅烷基组合物，其可以在低聚物合成的末端直接偶联或通过连接基团偶联到感兴趣的化合物，优选至低聚物。硅原子包括用作捕获标签的一个和三个侧链之间。在一个实施方案中，捕获标签是亲脂性的，其允许衍生的寡聚体通过反相色谱与失效序列分离。在另一个实施方案中，捕获标签是对其他化合物具有已知亲和力的化合物，其它化合物优选与固体支持物相关联以允许色谱分离。实例包括半抗原，抗体和配体。生物素可以与链亲和素结合的固体支持物结合或相互作用，是这种类型的优选捕获标签。

102. 发明授权

US06900180B1 Pharmaceutical compositions for alleviating discomfort 有权
标题翻译：减轻不适的药物组合物
公开(公告)号：US06900180B1
公开(公告)日：2005-05-31
申请号：US09889793
申请日：2000-01-20
申请人： Robert Johan Joseph Hageman , Jacob Geert Bindels
发明人： Robert Johan Joseph Hageman , Jacob Geert Bindels
IPC分类号： C07D475/04 , A23L1/29 , A23L1/302 , A23L1/304 , A23L1/305 , A61K31/14 , A61K31/198 , A61K31/255 , A61K31/405 , A61K31/409 , A61K31/44 , A61K31/4415 , A61K31/505 , A61K31/51 , A61K31/519 , A61K31/7076 , A61K31/714 , A61K33/06 , A61K33/30 , A61K38/41 , A61P3/02 , A61P25/00 , A61P25/22 , C07D209/20 , C07D213/67 , C07D417/06 , C07H19/167 , C07H23/00 , A61K31/70 , A61K31/195 , A61K31/50
CPC分类号： A61K38/02 , A23L33/15 , A23L33/155 , A23L33/16 , A23L33/175 , A23L33/19 , A23L33/40 , A23V2002/00 , A61K31/4415 , A61K31/519 , A61K31/714 , A61K33/30 , Y10S426/801 , A23V2250/54246 , A23V2250/54252 , A23V2250/065 , A23V2250/0616 , A23V2250/7052 , A23V2250/706 , A23V2250/1614 , A23V2250/16 , A23V2250/1582 , A23V2250/1578 , A23V2250/1618 , A23V2250/161 , A23V2250/1592 , A23V2250/156 , A23V2250/1872 , A23V2250/1874 , A23V2250/708 , A23V2250/702 , A23V2250/71 , A23V2250/712 , A23V2250/704 , A23V2250/705 , A23V2250/70 , A23V2250/612 , A23V2250/186 , A23V2250/5114 , A23V2250/0606 , A23V2250/211 , A23V2250/0644 , A61K2300/00 , A23V2200/322 , A23V2200/31 , A23V2250/7056 , A23V2250/54 , A23V2250/1868 , A23V2250/60
摘要： The invention relates to products for complete nutrition of infants or diseased or elderly persons. The products are characterized by increased levels of folic acid, vitamin B6 and vitamin B12 or their functional equivalents. These products improve feelings of well-being of infants, especially those of young age, and are useful in the treatment and prevention of diseases that are associated with disorders of serotonin and melatonin metabolism.
摘要翻译：本发明涉及婴幼儿或患病或老年人完全营养的产品。产品的特点是叶酸，维生素B6和维生素B12的水平升高或其功能等同物。这些产品改善了婴儿，特别是年轻人的幸福感，并且可用于治疗和预防与5-羟色胺和褪黑素代谢紊乱相关的疾病。

103. 发明授权

US6114513A Thiol-derivatized oligonucleotides 失效
标题翻译：硫醇衍生的寡核苷酸
公开(公告)号：US6114513A
公开(公告)日：2000-09-05
申请号：US924326
申请日：1997-09-05
申请人： Phillip Dan Cook , Muthiah Manoharan
发明人： Phillip Dan Cook , Muthiah Manoharan
IPC分类号： A61K38/00 , A61K48/00 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , C07J43/00 , C07J51/00 , C12N9/02 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C07J51/00 , C12N15/1137 , C12N9/0069 , C12Q1/68 , C12Y113/11012 , A61K38/00 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Nucleosides and linked nucleosides functionalized to include alkylthiol chemical functionality at ribofuranosyl positions, nucleosidic base positions, or on internucleoside linkages. In certain embodiments, the compounds of the invention further include steroids, reporter molecules, reporter enzymes, lipophilic molecules, peptides or proteins attached to the nucleosides through the alkylthio group.
摘要翻译：核苷和连接的核苷被功能化以包括在呋喃核糖基位置，核苷碱基位置或核苷间键上的烷硫醇化学官能度。在某些实施方案中，本发明的化合物还包括通过烷硫基连接到核苷上的类固醇，报道分子，报道酶，亲脂性分子，肽或蛋白质。

104. 发明授权

US6087100A Nucleic acid mediated electron transfer 失效
公开(公告)号：US6087100A
公开(公告)日：2000-07-11
申请号：US946679
申请日：1997-10-08
申请人： Thomas J. Meade , Jon Faiz Kayyem , Scott E. Fraser
发明人： Thomas J. Meade , Jon Faiz Kayyem , Scott E. Fraser
IPC分类号： C12N15/09 , C07H21/00 , C07H23/00 , C12Q1/68 , G01N33/566 , C07H21/04
CPC分类号： C07H23/00 , C07H21/00 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.

105. 发明授权

US6071699A Nucleic acid mediated electron transfer 失效
公开(公告)号：US6071699A
公开(公告)日：2000-06-06
申请号：US100507
申请日：1998-06-19
申请人： Thomas J. Meade , Jon Faiz Kayyem , Scott E. Fraser
发明人： Thomas J. Meade , Jon Faiz Kayyem , Scott E. Fraser
IPC分类号： C07H21/00 , C07H23/00 , C12Q1/68 , C07H21/04 , G01N27/00 , G01N33/483
CPC分类号： C12Q1/6825 , C07H21/00 , C07H23/00 , C12Q1/6818
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.

106. 发明授权

US6048695A Chemically modified nucleic acids and methods for coupling nucleic acids to solid support 失效
标题翻译：化学修饰的核酸和将核酸耦合到固体支持物的方法
公开(公告)号：US6048695A
公开(公告)日：2000-04-11
申请号：US71876
申请日：1998-05-04
申请人： Allan Bradley , Wei Wen Cai
发明人： Allan Bradley , Wei Wen Cai
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07H23/00 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N37/00
CPC分类号： C12Q1/6806 , C07H21/00 , C07B2200/11 , C12Q1/6837 , C12Q2525/197 , C40B40/00
摘要： The invention relates to novel chemically modified nucleic acids with enhanced lability towards solid supports, such as glass. These modified nucleic acids can be readily affixed to solid supports, for instance, a glass surface, without first derivatizing the glass surface. High-density microarrays based on these modified nucleic acids as well as methods for preparing these microarrays are also useful.
摘要翻译：本发明涉及对固体支持物例如玻璃具有增强的不稳定性的新化学修饰的核酸。这些修饰的核酸可以容易地固定到固体支持物，例如玻璃表面，而不首先使玻璃表面衍生化。基于这些修饰的核酸的高密度微阵列以及制备这些微阵列的方法也是有用的。

107. 发明授权

US5998135A Energy transfer hybridization assay using intercalators and lanthanide metals 失效
标题翻译：能量转移杂交测定使用嵌入剂和镧系金属
公开(公告)号：US5998135A
公开(公告)日：1999-12-07
申请号：US486053
申请日：1995-06-07
申请人： Elazar Rabbani , Ian Hurley
发明人： Elazar Rabbani , Ian Hurley
IPC分类号： C07H21/00 , C07H23/00 , C12Q1/68 , C07H21/04
CPC分类号： C07H23/00 , C07H21/00 , C12Q1/6818 , C12Q1/6825
摘要： Disclosed is a nucleic acid hybridization assay composition for detecting the presence of absence of a target oligo- or polynucleotide in a sample. The composition comprises: a solid matrix having at least one surface which is substituted with a first intercalator capable of binding dsDNA dsRNA, or DAN-RNA hybrids; a second intercalator, which may or may not comprise at least one fluorophore, said intercalator or said fluorophore each acting as either an energy donor or an energy acceptor; and an oligo- or polynucleotide probe which is specifically hybridizable with the target oligo- or polynucleotide and has directly or indirectly bound thereto, at least one lanthanide metal chelate or at least one fluorophore, each acting as either an energy donor or an energy acceptor. Also disclosed are a method and kit for its use.
摘要翻译：公开了用于检测样品中目标寡聚物或多核苷酸不存在的核酸杂交测定组合物。所述组合物包含：固体基质，其具有被能够结合dsDNA dsRNA的第一嵌入剂或DAN-RNA杂交体取代的至少一个表面; 第二嵌入剂，其可以或可以不包含至少一个荧光团，所述嵌入剂或所述荧光团各自用作能量供体或能量受体; 和与目标寡聚物或多核苷酸特异性杂交并与其直接或间接结合的寡核苷酸或多核苷酸探针，至少一种镧系元素金属螯合物或至少一种荧光团，其各自作为能量供体或能量受体。还公开了其使用的方法和试剂盒。

108. 发明授权

US5952478A Intermediates for dinucleotide and oligonucleotide analogues 失效
公开(公告)号：US5952478A
公开(公告)日：1999-09-14
申请号：US793725
申请日：1997-04-14
申请人： Anthony David Baxter , Roger John Taylor , Stephen Paul Collingwood
发明人： Anthony David Baxter , Roger John Taylor , Stephen Paul Collingwood
IPC分类号： A61K31/70 , A61K31/7024 , A61K31/7042 , A61K31/7048 , A61K31/7052 , A61K31/7064 , A61K31/7068 , A61K31/7072 , A61K31/7076 , A61K31/7135 , A61P31/12 , A61P35/00 , A61P43/00 , C07D405/04 , C07F9/32 , C07F19/00 , C07H9/04 , C07H13/02 , C07H13/04 , C07H13/06 , C07H13/08 , C07H19/01 , C07H19/04 , C07H19/06 , C07H19/16 , C07H21/00 , C07H23/00 , C07H19/00 , A01N43/04 , C07H21/02 , C07H21/04
CPC分类号： C07H9/04 , C07H13/04 , C07H13/08 , C07H19/04 , C07H21/00 , Y02P20/55
摘要： A compound of formula (I) or salts thereof, where R.sup.0 is hydrogen or together with R.sup.7 O denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, R.sup.1 is hydrogen, R.sup.1.sub.a or a group of formula (II), R.sup.1.sub.a is R.sup.1.sub.b or a protecting group Q, R.sup.1.sub.b is C.sub.1 -C.sub.20 alkyl, C.sub.2 -C.sub.20 alkenyl, C.sub.3 -C.sub.10 cycloalkyl, C.sub.6 -C.sub.15 aryl, C.sub.7 -C.sub.16 aralkyl or a 5- or 6-membered heterocyclic group attached by a carbon atom in the heterocyclic group to the indicated phosphorus atom, R.sup.2 is R.sup.2.sub.a or --OR.sup.15 or together with R.sup.5 denotes an oxy group --O--, provided that R.sup.2 is --OR.sup.15 when R.sup.1 is a group of formula (II), R.sup.2.sub.a is a C.sub.1 -C.sub.20 aliphatic group, a C.sub.3 -C.sub.10 cycloaliphatic group, a C.sub.6 -C.sub.15 aromatic group, a C.sub.7 -C.sub.16 araliphatic group, or a 5- or 6-membered heterocyclic group attached by a carbon atom in the heterocyclic group to the indicated phosphorus atom, R.sup.3 is R.sup.3.sub.a or Z, R.sup.3.sub.a is hydrogen, halogen, hydroxy, R.sup.16, --OR.sup.16, OCOR.sup.16, --OSO.sub.2 R.sup.16, or tri(C.sub.1 -C.sub.15 hydrocarbyl)silyloxy, R.sup.4 is hydrogen, halogen or R.sup.17, R.sup.5 is R.sup.5.sub.a or Z or together with R.sup.2 denotes an oxy group --O--, R.sup.5.sub.a is hydrogen, halogen, hydroxy, R.sup.18, --OR.sup.18, --OCOR.sup.18, --OSO.sub.2 R.sup.18, or tri(C.sub.1 -C.sub.15 hydrocarbyl)silyloxy, or together with R.sup.6 denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, R.sup.6 is halogen, hydroxy, --OR.sup.19, --OCOR.sup.19, --OSO.sub.2 R.sup.19 or B.sup.1, or together with R.sup.5 denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, provided that R.sup.6 is not B.sup.1 when R.sup.1 is a group of formula (II) in which R.sup.14 is B.sup.2, R.sup.7 is hydrogen or R.sup.7.sub.a, R.sup.7.sub.a is R.sup.20, --COR.sup.20, --SO.sub.2 R.sup.20 or tri(C.sub.1 -C.sub.15 hydrocarbyl)silyl, or together with the attached oxygen atom and R.sup.0 denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, R.sup.8 is hydrogen, halogen, hydroxy, R.sup.21, --OR.sup.21, --OCOR.sup.21, --OSO.sub.2 R.sup.21 or together with R.sup.10 denotes a valence bond, R.sup.9 is hydrogen, halogen or R.sup.22, R.sup.10 is hydrogen, halogen, hydroxy, R.sup.23, --OR.sup.23, --OCOR.sup.23 or --OSO.sub.2 R.sup.23, or together with R.sup.8 denotes a valence bond, R.sup.11 is hydrogen, halogen or R.sup.24, R.sup.12 is hydrogen or R.sup.12.sub.a, or R.sup.12 O-- together with R.sup.13 denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, R.sup.12.sub.a is R.sup.25, --COR.sup.25, --SO.sub.2 R.sup.25 or tri(C.sub.1 -C.sub.15 hydrocarbyl)silyl, R.sup.13 is R.sup.13.sub.a or Z, R.sup.13.sub.a is hydrogen, halogen, hydroxy, R.sup.26, --OR.sup.26, --OCOR.sup.26, --OSO.sub.2 R.sup.26 or tri(C.sub.1 -C.sub.15 hydrocarbyl)silyloxy, or together with R.sup.14 denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, or together with R.sup.12 O-- denotes a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, R.sup.14 is halogen, hydroxy, --OR.sup.27, --OCOR.sup.27, --OSO.sub.2 R.sup.27, or B.sup.2 or together with R.sup.13 denoted a C.sub.1 -C.sub.15 hydrocarbylidenedioxy group, provided that R.sup.14 is not B.sup.2 when R.sup.6 is B.sup.1, R.sup.15 is hydrogen or R.sup.15.sub.a, R.sup.15.sub.a is a C.sub.1 to C.sub.10 aliphatic group, a C.sub.3 to C.sub.8 cycloaliphatic group, a C.sub.6 to C.sub.15 aromatic group or a C.sub.7 to C.sub.16 araliphatic group, R.sup.16, R.sup.17, R.sup.18, R.sup.19, R.sup.20, R.sup.21, R.sup.22, R.sup.23, R.sup.24, R.sup.25, R.sup.26 and R.sup.27 are independently a C.sub.1 to C.sub.10 aliphatic group, a C.sub.3 to C.sub.10 cycloaliphatic group, a C.sub.6 to C.sub.15 aromatic group or a C.sub.7 to C.sub.30 araliphatic group, B.sup.1 and B.sup.2 are independently each a monovalent nucleoside base radical, and Z is substituted or unsubstituted C.sub.6 to C.sub.10 aryloxythiocarbonyloxy. ##STR1##

109. 发明授权

US5856515A Electron-deficient porphyrins and processes and intermediates for preparing same 失效
公开(公告)号：US5856515A
公开(公告)日：1999-01-05
申请号：US763766
申请日：1996-12-11
申请人： Michael J. Therien , Stephen DiMagno
发明人： Michael J. Therien , Stephen DiMagno
IPC分类号： A61K31/409 , A61K31/555 , A61K41/00 , A61K47/48 , A61K49/00 , A61K51/04 , A61L2/08 , A61L2/18 , A61P31/04 , B01J27/10 , B01J27/12 , B01J31/02 , B01J31/18 , B01J31/22 , B01J31/24 , C07C29/50 , C07D207/32 , C07D207/33 , C07D207/333 , C07D207/335 , C07D487/22 , C07D513/22 , C07D519/00 , C07F3/00 , C07F7/08 , C07F7/10 , C07H23/00 , C08F8/42 , C08F30/04 , C08F38/00 , C08G61/12 , C08G73/06 , C08G79/00 , C08G83/00 , C09B47/00 , C09B47/08 , C09B69/10 , G01N27/12 , G01N27/40 , G03G5/06 , G03G5/07 , C07D209/00 , C07D207/00
CPC分类号： A61K47/48069 , A61K31/555 , A61K41/0071 , A61K47/48023 , A61K51/0485 , B01J31/0244 , B01J31/1805 , B01J31/1815 , B01J31/2409 , C07C29/50 , C07D207/33 , C07D207/333 , C07D207/335 , C07D487/22 , C07F7/082 , C07F7/0832 , C08F30/04 , C08G73/0611 , C08G79/00 , C09B47/00 , C09B69/10 , C09B69/108 , G01N27/126 , G03G5/0696 , G03G5/076 , B01J2231/42 , B01J2231/70 , B01J2231/72 , B01J2531/025 , B01J2531/26 , B01J2531/824 , B01J2531/922 , B01J2531/96
摘要： Electron-deficient porphyrins are provided, as well as processes and intermediates for their preparation. In preferred embodiments, the electron-deficient porphyrins are prepared by condensing pyrrole derivatives and removing water thus formed from the resulting reaction mixture.

110. 发明授权

US5856466A Process for the preparation of 2'-O-alkyl purine phosphoramidites 失效
公开(公告)号：US5856466A
公开(公告)日：1999-01-05
申请号：US888880
申请日：1997-07-07
申请人： Phillip Dan Cook , Daniel Peter Claude McGee , Charles John Guinosso
发明人： Phillip Dan Cook , Daniel Peter Claude McGee , Charles John Guinosso
IPC分类号： A61K38/00 , A61K48/00 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/00 , C12N9/02 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/1137 , C12N9/0069 , C12Q1/68 , C12Y113/11012 , A61K38/00 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Processes for preparing 2'-O-alkylated guanosine, uridine, cytidine, and 2,6-diaminopurine 3'-O-phosphoramidites include the steps of alkylating nucleoside precursors, adding suitable blocking groups and phosphitylating. For the guanosine 2'-O-alkylated 3'-O-phosphoramidites, alkylation is effected on 2,6-diamino-9-(.beta.-D-ribofuranosyl)purine followed by deamination. For uridine 2'-O-alkylated 3'-O-phosphoramidites, alkylation is effect on a dialkyl stannylene derivative of uridine. For cytidine 2'-O-alkylated 3'-O-phosphoramidites, alkylation is effected directly on cytidine. Alkylation is effected directly upon 2,6-diaminopurine.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式