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101. 发明申请

US20070254359A1 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS 有权
标题翻译：人类胚胎干细胞的分化
公开(公告)号：US20070254359A1
公开(公告)日：2007-11-01
申请号：US11736908
申请日：2007-04-18
申请人： Alireza Rezania , Jean Xu
发明人： Alireza Rezania , Jean Xu
IPC分类号： C12N5/08
CPC分类号： C12N5/0676 , A61K35/39 , C12N5/067 , C12N2501/115 , C12N2501/119 , C12N2501/16 , C12N2501/385 , C12N2501/415 , C12N2501/42 , C12N2501/70 , C12N2501/727 , C12N2506/02 , C12N2533/90
摘要： The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
摘要翻译：本发明提供促进多能干细胞分化的方法。特别地，本发明提供了形成胰腺内胚层，表达胰腺激素的细胞和分泌胰腺分泌细胞的改进方法。本发明还提供了在不使用饲养细胞层的情况下促进多能干细胞的分化的方法。

102. 发明授权

US07282366B2 Hepatocytes for therapy and drug screening made from embryonic stem cells 有权
标题翻译：用胚胎干细胞进行治疗和药物筛选的肝细胞
公开(公告)号：US07282366B2
公开(公告)日：2007-10-16
申请号：US10087142
申请日：2002-03-01
申请人： Lakshmi Rambhatla , Melissa K. Carpenter
发明人： Lakshmi Rambhatla , Melissa K. Carpenter
IPC分类号： C12N5/00 , C12N5/02 , C12N5/06 , C12N5/10 , C12N5/08
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/92
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
摘要翻译：已经发现，当在肝细胞分化剂存在下培养多能干细胞时，衍生出具有非常高比例的具有肝细胞表型特征的细胞的细胞群。在一个实例中，允许人胚胎干细胞形成胚状体，然后与分选剂正丁酸酯组合，任选地补充成熟因子。在另一个实例中，在无饲养细胞培养物中将正丁酸加入到人胚胎干细胞中。无论哪种方式，获得了显着均匀的细胞群，其由具有肝细胞形态特征的细胞占主导，表达肝细胞特征的表达标志物，并具有对肝功能重要的酶和生物合成活性。由于干细胞在培养物中容易增殖，所以该系统为各种应用提供了丰富的肝细胞谱系来源，如药物筛选，并在临床治疗的背景下补充肝功能。

103. 发明申请

US20070196345A1 Antibody recognizing proliferative human liver cells, proliferative human liver cells and functional human liver cells 审中-公开
公开(公告)号：US20070196345A1
公开(公告)日：2007-08-23
申请号：US11723529
申请日：2007-03-20
申请人： Chise Mukaidani , Katsutoshi Yoshizato , Chihiro Yamasaki
发明人： Chise Mukaidani , Katsutoshi Yoshizato , Chihiro Yamasaki
IPC分类号： A61K35/407 , C12N5/08
CPC分类号： C12N5/067 , C07K16/18 , C07K16/28 , C12N5/0697 , C12N2501/38 , C12N2510/00
摘要： The invention provides a monoclonal antibody which specifically recognizes proliferative human hepatocytes, a method for isolating proliferative human hepatocytes by using the antibody, and a method for inducing to differentiate the proliferative human hepatocytes to functional human hepatocytes. Further, the invention provides the proliferative human hepatocytes and functional human hepatocytes obtained by the methods, as well as a cell kit and a hybrid artificial liver comprising those hepatocytes.

104. 发明授权

US07256042B2 Process for making hepatocytes from pluripotent stem cells 有权
标题翻译：从多能干细胞制备肝细胞的方法
公开(公告)号：US07256042B2
公开(公告)日：2007-08-14
申请号：US10001267
申请日：2001-10-31
申请人： Lakshmi Rambhatla , Melissa K. Carpenter
发明人： Lakshmi Rambhatla , Melissa K. Carpenter
IPC分类号： C12N5/00 , C12N5/08 , C12N5/02
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/148 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture. Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
摘要翻译：已经发现，当在肝细胞分化剂存在下培养多能干细胞时，衍生出具有非常高比例的具有肝细胞表型特征的细胞的细胞群。在一个实例中，允许人胚胎干细胞形成胚状体，然后与分选剂正丁酸酯组合，任选地补充成熟因子。在另一个实例中，在无饲养细胞培养物中将正丁酸加入到人胚胎干细胞中。无论哪种方式，获得了显着均匀的细胞群，其由具有肝细胞形态特征的细胞占主导，表达肝细胞特征的表达标志物，并具有对肝功能重要的酶和生物合成活性。由于干细胞在培养物中容易增殖，所以该系统为各种应用提供了丰富的肝细胞谱系来源，如药物筛选，并在临床治疗的背景下补充肝功能。

105. 发明申请

US20070072291A1 Dedifferentiated, programmable stem cells of monocytic origin, and their production and use 审中-公开
标题翻译：单分散来源的分化型可编程干细胞及其生产和使用
公开(公告)号：US20070072291A1
公开(公告)日：2007-03-29
申请号：US11543922
申请日：2006-10-06
申请人： Bernd Kremer , Fred Fandrich , Maren Schulze
发明人： Bernd Kremer , Fred Fandrich , Maren Schulze
IPC分类号： C12N5/02 , C12N5/00
CPC分类号： A61L27/3886 , A61K35/12 , A61K2035/124 , A61L27/3804 , A61L27/3834 , A61L27/3895 , C12N5/0607 , C12N5/0618 , C12N5/0619 , C12N5/0622 , C12N5/0629 , C12N5/0647 , C12N5/0653 , C12N5/067 , C12N5/0676 , C12N5/069 , C12N2500/30 , C12N2500/44 , C12N2501/10 , C12N2501/11 , C12N2501/117 , C12N2501/119 , C12N2501/12 , C12N2501/20 , C12N2501/22 , C12N2501/23 , C12N2501/235 , C12N2501/33 , C12N2501/385 , C12N2501/39 , C12N2502/14 , C12N2506/03 , C12N2506/11 , C12N2510/00
摘要： The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for the production of target cells and target tissue.
摘要翻译：本发明涉及通过在含有M-CSF和IL-3的培养基中培养单核细胞从人单核细胞生产成人去分化的可编程干细胞。本发明还涉及含有去分化的可编程干细胞的药物制剂，以及这些干细胞用于产生靶细胞和靶组织的用途。

106. 发明授权

US07186553B1 Human derived immortalized liver cell line 有权
标题翻译：人源性永生化肝细胞系
公开(公告)号：US07186553B1
公开(公告)日：2007-03-06
申请号：US09673958
申请日：1999-04-27
申请人： Masayoshi Nanba , Kenichi Fukaya , Satoru Asahi , Sumie Yoshitomi
发明人： Masayoshi Nanba , Kenichi Fukaya , Satoru Asahi , Sumie Yoshitomi
IPC分类号： C12N5/08 , C12N5/00 , C12N5/02
CPC分类号： C12Q1/48 , C12N5/067 , C12N2503/02 , C12N2510/04 , C12Q1/26 , C12Q1/32
摘要： The present invention relates to a new immortalized hepatocyte culture of human (preferably human fetal) normal cell origin. The immortalized hepatocyte culture of human normal cell origin of the present invention is useful in, for example, screening for compounds or salts thereof having therapeutic/preventive effects on hepatic insufficiency.
摘要翻译：本发明涉及人（优选人胎儿）正常细胞来源的新的永生化肝细胞培养物。本发明的人正常细胞来源的永生化肝细胞培养物可用于例如筛选其对肝功能不全具有治疗/预防作用的化合物或其盐。

107. 发明申请

US20060166360A1 Compositions, solutions, and methods used for transplantation 审中-公开
标题翻译：用于移植的组合物，解决方案和方法
公开(公告)号：US20060166360A1
公开(公告)日：2006-07-27
申请号：US10531001
申请日：2003-10-17
申请人： Francois Berthiaume , Martin Yarmush , Yasuji Mokuno
发明人： Francois Berthiaume , Martin Yarmush , Yasuji Mokuno
IPC分类号： C12N5/08
CPC分类号： C12N5/067 , A01N1/02 , A01N1/0226 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2501/81
摘要： This invention discloses a method for reducing the intracellular lipid storage material of a cell, tissue, or organ for transplantation and features solutions, methods and kits that induce the metabolic elimination of lipid storage in a cell, tissue, or organ. In one exemplary approach, the process involves contacting a cell, tissue, or organ with a perfusate solution that include catabolic hormones and amino acids, at physiological conditions, to increase lipid export and lipid oxidation. If desired, the cell, tissue, or organ of the invention may also be heat shock preconditioned. The invention can be used to prepare, recondition, or store a cell, tissue, or organ for transplantation by increasing tolerance to ischemia-reperfusion and cold-preservation related injury.
摘要翻译：本发明公开了一种减少用于移植的细胞，组织或器官的细胞内脂质储存材料的方法，其特征在于诱导脂质储存在细胞，组织或器官中的代谢消除的溶液，方法和试剂盒。在一个示例性方法中，该方法包括在生理条件下使细胞，组织或器官与包括分解代谢激素和氨基酸的灌注液接触，以增加脂质输出和脂质氧化。如果需要，本发明的细胞，组织或器官也可以是热休克预处理的。本发明可用于通过增加对缺血再灌注和冷保存相关损伤的耐受性来制备，修复或储存用于移植的细胞，组织或器官。

108. 发明申请

US20060160216A1 Human liver cell line 有权
公开(公告)号：US20060160216A1
公开(公告)日：2006-07-20
申请号：US11335352
申请日：2006-01-19
申请人： Jetsumon Prachumsri , Nongnuch Yimamnuaychok , Walter Beerheide
发明人： Jetsumon Prachumsri , Nongnuch Yimamnuaychok , Walter Beerheide
IPC分类号： C12N5/08 , C12N1/10
CPC分类号： C12N5/067 , C12N2500/05 , C12N2500/30 , C12N2500/36 , C12N2500/38 , C12N2500/62 , C12N2501/30 , C12N2501/335 , C12N2501/375 , C12N2501/39 , C12N2503/00 , C12N2503/02 , C12N2510/02 , C12N2510/04
摘要： In this application is described the establishment and maintanence of a normal human hepatocyte cell line able to support complete development of malaria parasite development in vitro. Advantages and uses of the cell line are also described.

109. 发明申请

US20060154235A1 Human hepatocyte-like cells and uses thereof 审中-公开
标题翻译：人肝细胞样细胞及其用途
公开(公告)号：US20060154235A1
公开(公告)日：2006-07-13
申请号：US11210337
申请日：2005-08-23
申请人： Takahiro Ochiya , Takumi Teratani
发明人： Takahiro Ochiya , Takumi Teratani
IPC分类号： C12Q1/00 , C12N5/08
CPC分类号： C12N5/067 , C12N2501/39 , C12Q1/18 , G01N33/5014 , G01N33/5038 , G01N33/5067 , G01N2800/08
摘要： The present invention provides modified methods of producing human hepatocyte-like cells which exhibit phenotypes more similar to those of human hepatocytes. The present invention also provides the human hepatocyte-like cells and uses thereof. The methods of the present invention comprises a prolonged incubation period and addition of dexamethasone to the culture medium. The methods of the inventors produce human hepatocyte-like cells whose morphology is more similar to that of human hepatocytes, compared with the conventional system. The cells produced have both morphological and functional features of primary culture cells from normal human liver, including cytochrome P450 (CYP), multi-drug resistance-associated protein (MRP), and multi-drug protein (MDR). The use of human hepatocyte-like cells of the present invention enables, without using any animal model, assessment of metabolism and hepatotoxicity of test compounds, which are drug candidates, and screening for therapeutic agents for hepatic diseases, inhibitors to hepatitis virus infection, and therapeutic agents for viral hepatitis.
摘要翻译：本发明提供了生产人肝细胞样细胞的修饰方法，其表现出与人肝细胞更相似的表型。本发明还提供人肝细胞样细胞及其用途。本发明的方法包括延长的潜伏期，并向培养基中加入地塞米松。与常规系统相比，本发明人的方法产生的人类肝细胞样细胞的形态与人肝细胞的形态更相似。所产生的细胞具有来自正常人肝脏的原代培养细胞的形态和功能特征，包括细胞色素P450（CYP），多药耐药相关蛋白（MRP）和多药物蛋白（MDR）。使用本发明的人类肝细胞样细胞能够在不使用任何动物模型的情况下评估作为药物候选物的测试化合物的代谢和肝毒性，以及筛选肝脏疾病的治疗剂，肝炎病毒感染的抑制剂和病毒性肝炎治疗剂。

110. 发明申请

US20050244966A1 Insulin producing cells 审中-公开
标题翻译：胰岛素生产细胞
公开(公告)号：US20050244966A1
公开(公告)日：2005-11-03
申请号：US11174480
申请日：2005-07-06
申请人： Shimon Efrat
发明人： Shimon Efrat
IPC分类号： A61K35/12 , A61P5/48 , C12N5/071 , C12N5/08
CPC分类号： C12N5/067 , A61K35/12 , C12N5/0676 , C12N2500/90 , C12N2501/60 , C12N2502/14 , C12N2510/00 , C12N2510/04 , C12N2799/027
摘要： The present invention relates to a method for modifying liver cells such that they produce and store insulin. In addition, the invention relates to these modified cells and their use in the treatment of insulin-dependant diabetes.
摘要翻译：本发明涉及一种修饰肝细胞使其产生和储存胰岛素的方法。此外，本发明涉及这些修饰的细胞及其在治疗胰岛素依赖性糖尿病中的用途。

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