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91. 发明申请

US20110118126A1 METHODS OF CHEMOTYPE EVOLUTION 审中-公开
标题翻译：化学发光方法
公开(公告)号：US20110118126A1
公开(公告)日：2011-05-19
申请号：US12934265
申请日：2009-03-25
申请人： Stig Hansen , Daniel Erlanson
发明人： Stig Hansen , Daniel Erlanson
IPC分类号： C40B20/08 , C40B30/04 , C40B20/00
CPC分类号： G01N33/531 , C07C319/22 , C07C323/25 , C07D277/48 , C07D333/38 , C07D417/12 , C07D471/04 , C07D487/04 , C07D495/04 , C12Q1/485 , C12Y207/10001 , C12Y207/11001 , G01N33/573 , G01N2333/91205
摘要： Herein is described a method to rapidly screen a large chemical space for a compound that binds to a target protein through an iterative fragment assembly approach that can be performed at low reagent cost and without requiring purification of the assembled product. The method employs a library of test ligands each of which comprise a ‘bait’ molecule, which is known from prior art or prior screening to have some intrinsic affinity for the target protein, and a test moiety.
摘要翻译：本文描述了通过可以低试剂成本执行并且不需要净化组装产品的迭代片段装配方法来快速筛选与靶蛋白结合的化合物的大化学空间的方法。该方法使用测试配体文库，每个测试配体包含“诱饵”分子，其从现有技术已知或先前筛选以对靶蛋白具有某些固有的亲和力，以及测试部分。

92. 发明申请

US20090312188A1 SYSTEM AND METHOD FOR NUCLEIC ACIDS SEQUENCING BY PHASED SYNTHESIS 有权
标题翻译：通过PHASED合成序列测定核酸的系统和方法
公开(公告)号：US20090312188A1
公开(公告)日：2009-12-17
申请号：US12481799
申请日：2009-06-10
申请人： Reuven Duer , James Herron
发明人： Reuven Duer , James Herron
IPC分类号： C40B20/08 , C40B60/10
CPC分类号： G02B27/56 , B01J2219/00529 , B01J2219/00596 , B01J2219/00659 , B01J2219/00704 , B01J2219/00711 , B01J2219/00722 , B01L3/502715 , B01L2300/0654 , B01L2300/0819 , B82Y30/00 , C12N15/1068 , C12Q1/6869 , C12Q1/6874 , G01N21/6452 , G01N21/648 , G01N2021/6441 , G02B6/24 , C12Q2523/319 , C12Q2535/107 , C12Q2565/607
摘要： A system and methods of sequencing a nucleic acid by detecting the identity of a fluorescent nucleotide analogue incorporated at the 3′ end of a growing nucleic acid strand are provided. One method includes the steps of (a) immobilizing a plurality of complexes comprising a template nucleic acid, a primer configured to hybridize to the template and a polymerase, at a plurality of optical sensing sites of a substrate, wherein the substrate is part of a waveguide-based optical scanning system; (b) extending the primer by a single nucleotide with the polymerase and one or more fluorescent nucleotide analogues using a polymerase extension reaction, wherein each type of fluorescent nucleotide analogue comprises a unique fluorescent tag optionally configured to inhibit further primer extension and/or a blocking agent at the 3′ end and wherein incorporation of the fluorescent nucleotide analogue reversibly terminates the polymerase extension reaction; (c) detecting the unique tag of the fluorescent nucleotide analogue by optically scanning the substrate using the optical scanning system to identify the fluorescent nucleotide analogue incorporated by the polymerase reaction; (d) recording the results of the optical scanning of the substrate; (e) reversing the termination of the polymerase extension reaction by providing a photo-cleaving pulse of light to one or more of the optical sensing sites of the substrate to cleave the fluorescent tag or the blocking agent; and (f) repeating steps (b) through (e).
摘要翻译：提供了通过检测在生长的核酸链的3'末端引入的荧光核苷酸类似物的身份来测序核酸的系统和方法。一种方法包括以下步骤：（a）在衬底的多个光学感测位置处固定包含模板核酸的多个复合物，配置为与模板杂交的引物和聚合酶，其中所述底物是基于波导的光学扫描系统; （b）使用聚合酶延伸反应，使用聚合酶和一个或多个荧光核苷酸类似物将引物延伸单个核苷酸，其中每种类型的荧光核苷酸类似物包含独特的荧光标签，其可选地配置为抑制进一步的引物延伸和/或阻断 3'端的试剂，其中掺入荧光核苷酸类似物可逆地终止聚合酶延伸反应; （c）通过使用光学扫描系统光学扫描底物来检测荧光核苷酸类似物的唯一标记，以鉴定通过聚合酶反应引入的荧光核苷酸类似物; （d）记录基板的光学扫描的结果; （e）通过向衬底的一个或多个光学感测位置提供光切割脉冲以切割荧光标签或封闭剂来反转聚合酶延伸反应的终止; 和（f）重复步骤（b）至（e）。

93. 发明授权

US07566685B2 Methods and compositions for screening using diphtheria toxin constructs 有权
标题翻译：使用白喉毒素构建体进行筛选的方法和组合物
公开(公告)号：US07566685B2
公开(公告)日：2009-07-28
申请号：US11392434
申请日：2006-03-28
申请人： Todd M. Kinsella
发明人： Todd M. Kinsella
IPC分类号： C40B30/06 , C40B20/04 , C40B20/08
CPC分类号： C07K14/475 , C07K14/43595 , C07K14/70596
摘要： The invention relates to methods and compositions utilizing diphtheria toxin for screening purposes. The invention is particularly useful in screening for modulators of IgE synthesis, secretion and switch rearrangement.
摘要翻译：本发明涉及利用白喉毒素进行筛选的方法和组合物。本发明特别可用于筛选IgE合成，分泌和开关重排的调节剂。

94. 发明申请

US20080248959A1 GLYCOSYLATION OF PEPTIDES VIA O-LINKED GLYCOSYLATION SEQUENCES 审中-公开
公开(公告)号：US20080248959A1
公开(公告)日：2008-10-09
申请号：US11781896
申请日：2007-07-23
申请人： Shawn DeFrees
发明人： Shawn DeFrees
IPC分类号： C40B20/08 , C07K14/00 , C12N9/00 , C07K16/00 , A61K38/00 , C07H21/04 , C12N15/00 , C12N5/00 , C40B40/10 , C12N15/87 , C12P21/06 , C40B50/06 , C40B20/00
CPC分类号： C07K9/001 , A61K38/00 , A61K47/549 , A61K47/60 , A61K47/64 , A61K47/643 , A61K47/644 , C07K1/047 , C07K1/1077 , C12P21/005
摘要： The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.

95. 发明授权

US07214487B2 Methods for identifying compounds that modulate enzymatic activities by employing covalently bonded target-extender complexes with ligand candidates 有权
标题翻译：通过使用与配体候选物共价键合的靶向增殖物复合物鉴定调节酶活性的化合物的方法
公开(公告)号：US07214487B2
公开(公告)日：2007-05-08
申请号：US10374499
申请日：2003-02-25
申请人： Daniel A. Erlanson , Robert S. McDowell , Stig Hansen
发明人： Daniel A. Erlanson , Robert S. McDowell , Stig Hansen
IPC分类号： C12Q1/68 , C40B20/08 , C40B50/08
CPC分类号： G01N33/573 , C07K1/1072 , G01N2333/916 , G01N2500/02
摘要： The present invention relates to methods for identifying compounds that modulate enzymatic activity by contacting covalently bonded PTP-extender complexes or modified covalently bonded PTP-extender complexes with ligand candidates.
摘要翻译：本发明涉及通过将共价键合的PTP-扩增体复合物或经修饰的共价键合的PTP-扩增体复合物与配体候选物接触来鉴定调节酶活性的化合物的方法。

96. 发明授权

US6020199A Antisense modulation of PTEN expression 有权
标题翻译： PTEN表达的反义调制
公开(公告)号：US6020199A
公开(公告)日：2000-02-01
申请号：US358381
申请日：1999-07-21
申请人： Brett P. Monia , Lex M. Cowsert
发明人： Brett P. Monia , Lex M. Cowsert
IPC分类号： C12N15/09 , A61K31/711 , A61K38/00 , A61K48/00 , A61P3/10 , A61P43/00 , C12N15/113 , C12Q1/02 , C40B20/08 , C40B40/06 , C40B50/14 , C07H21/04 , C12N15/00 , C12Q1/68
CPC分类号： C40B50/14 , C12N15/1137 , C40B20/08 , C40B40/06 , A61K38/00 , C12N2310/315 , C12N2310/321 , C12N2310/3341 , C12N2310/341 , C12N2310/346 , Y02P20/582
摘要： Antisense compounds, compositions and methods are provided for modulating the expression of PTEN. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding PTEN. Methods of using these compounds for modulation of PTEN expression and for treatment of diseases associated with expression of PTEN are provided.
摘要翻译：提供反义化合物，组合物和方法用于调节PTEN的表达。组合物包含靶向编码PTEN的核酸的反义化合物，特别是反义寡核苷酸。提供了使用这些化合物调节PTEN表达和治疗与PTEN表达相关的疾病的方法。

97. 发明授权

US11421222B2 Bacterial delivery vehicles comprising tracer nucleic acid sequences 有权
公开(公告)号：US11421222B2
公开(公告)日：2022-08-23
申请号：US16905656
申请日：2020-06-18
申请人： Eligo Bioscience
发明人： Igor Stzepourginski , Daniel Garry
IPC分类号： C12N15/10 , C12N7/00 , C12Q1/70 , C40B20/08
摘要： The present disclosure relates generally to genetically tagged bacterial delivery vehicles comprising unique tracer nucleic acid sequences (herein referred to as “tracers”) for use in detecting and/or quantitating the presence of two or more different said bacterial delivery vehicles within a mixture of vehicles. The present disclosure relates to methods wherein the bacterial delivery vehicles are detected through, for example, performance of multiple cycles of amplification using primers that bind to sequences within the unique tracer. Such methods can be advantageously used in quality control to detect and quantitate mixtures of bacterial delivery vehicles within a pharmaceutical composition.

98. 发明申请

US20220170021A1 OLIGONUCLEOTIDE PROBES AND USES THEREOF 有权
公开(公告)号：US20220170021A1
公开(公告)日：2022-06-02
申请号：US17227009
申请日：2021-04-09
申请人： Caris Science, Inc.
发明人： Valeriy Domenyuk , Adam Stark , Nianqing Xiao , Zhenyu Zhong , Mark Miglarese , David Spetzler
IPC分类号： C12N15/115 , C12Q1/6886 , C40B20/08 , G01N33/50
摘要： Methods and compositions are provided for oligonucleotide probes and oligonucleotide probe libraries that recognize targets of interest. The targets include circulating biomarkers such as microvesicles, including those derived from various diseases.

99. 发明授权

US10822605B2 Method and apparatus for producing sequence verified DNA 有权
公开(公告)号：US10822605B2
公开(公告)日：2020-11-03
申请号：US14268832
申请日：2014-05-02
申请人： GENSINTA, INC.
发明人： Austen Heinz , Anselm Levskaya , John T. Mulligan
IPC分类号： C12N15/10 , C40B20/08 , C40B50/14
摘要： A method of retrieving a subset of polynucleotide molecules from a mixture of polynucleotide molecules includes receiving a mixture of nucleotide sequences comprising one or more polynucleotide molecules, synthesizing one or more identifier (ID) regions onto the one or more polynucleotide molecules, and sequencing members of the population of polynucleotide molecules to associate the sequence of one or more of the molecules (the “Polynucleotide Sequence”) with the sequence of the attached ID region (the “ID Sequence”). The method also includes generating a bead-bound library of one or more beads comprising subsets of identical polynucleotide molecules. Each bead is identified by the ID Sequence of the associated Polynucleotide Sequence. The method further includes sequencing the one or more ID regions of each bead to generate ID Sequence information for each bead, combining the Polynucleotide Sequence information, the one or more ID Sequences, and coordinates of each bead to identify the Polynucleotide Sequence on the bead, and retrieving the bead with its associated Polynucleotide Sequence from the flow cell based on the absolute coordinate position of the bead.

100. 发明申请

US20200158709A1 THERAPEUTIC DRUG FOR LIPID-PEROXIDATION-INDUCED DISEASES AND SCREENING METHOD FOR THERAPEUTIC DRUGS FOR LIPID-PEROXIDATION-INDUCED DISEASES 审中-公开
公开(公告)号：US20200158709A1
公开(公告)日：2020-05-21
申请号：US16628446
申请日：2018-07-05
申请人： Ken-ichi YAMADA , FUSO PHARMACEUTICAL INDUSTRIES, LTD.
发明人： Ken-ichi YAMADA , Saki SHINTO , Tomomi IDE , Keiichi YAMAMOTO
IPC分类号： G01N33/15 , G01N33/52 , C07D413/12 , C01G49/14 , C40B20/08 , C12N5/071 , C07C57/00
摘要： The present invention provides: an assay method that uses a compound represented by formula (I) as a fluorescent probe molecule and that is for detecting the lipid peroxidation suppression activity of a test compound; an assay kit that uses the assay method; a screening method that uses the assay method; and a pharmaceutical composition that is for the treatment, etc. of diseases (such as age-related macular degeneration) that are induced by lipid peroxidation reactions.

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