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    • 93. 发明授权
    • Medical diagnostic method
    • 医疗诊断方法
    • US3399971A
    • 1968-09-03
    • US36725064
    • 1964-05-13
    • PFIZER & CO C
    • KITA DONALD A
    • A61B5/15G01N30/94G01N33/64G01N33/68
    • G01N33/6812A23V2002/00G01N30/94G01N33/64A23V2250/0638
    • A diagnostic test pack for determining phenylalanine in blood (e.g. for detection of phenylketonuria) comprises an absorbent carrier strip to which serum to be tested may be applied and a developer comprising 0.3-1.0% ninhydrin dissolved in the separated upper layer formed when butanol and aqueous acetic acid are mixed and allowed to stand. The carrier is preferably paper, but any bibulous material, e.g. made of cellulose, modified cellulose, polyvinyl chloride, or glass fibres may be used. In use, a serum containing a known amount of alanine is preferably spotted, with the serum to be tested, on the test strip, the sera allowed to dry, and the spots developed by ascending, descending, circular, disc or radial chromatography. After development and drying, the colour intensity of the furthest advanced spots, is compared.
    • 用于测定血液中苯丙氨酸的诊断测试包(例如用于检测苯丙酮尿症)包括吸收性载体条,待测试的血清可以被施用到该吸收性载体条带上,并且包含0.3-1.0%的茚三酮的显影剂溶于分离的上层,当丁醇和水溶液 乙酸混合并静置。 载体优选是纸,但任何吸水材料,例如, 可以使用纤维素,改性纤维素,聚氯乙烯或玻璃纤维。 在使用中,优选含有已知量的丙氨酸的血清,待测血清,试纸上的血清,允许干燥的血清,以及通过上升,下降,圆形,圆盘或径向色谱法显影的斑点。 在开发和干燥后,比较最先进的斑点的颜色强度。