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91. 发明申请

US20040018495A1 Microparticle based signal amplification for the detection of analytes 审中-公开
标题翻译：用于检测分析物的基于微粒的信号放大
公开(公告)号：US20040018495A1
公开(公告)日：2004-01-29
申请号：US10205195
申请日：2002-07-24
发明人： Xing-Xiang Li
IPC分类号： C12Q001/68
CPC分类号： C07H21/02 , C12Q1/682 , C12Q2563/149 , C12Q2563/143 , C12Q2537/125
摘要： Microparticle based amplification (MBA) for high sensitivity and high speed analyte detection is described. MBA is based on signal amplification achieved by use of a signal amplification microparticle that contains a plurality of signaling molecules attached to a plurality of positions on the surface of the microparticle, in combination with a plurality of analyte binding molecules attached to a plurality of positions on the surface. Each signaling molecule in turn has a plurality of signal emitting moieties, such as acridinium, attached thereto. This is combined with a separating microparticle such as a ferromagnetic particle, also having an analyte binding molecule attached to the surface so that a complex comprising the analyte, the signal amplification microparticle and the separating microparticle is formed. The complex emits a signal that is amplified many fold relative to the stoichemetric amount of analyte molecules in the sample. Particular embodiments include methods for detecting bacteria, antigens, antibodies and nucleic acids.
摘要翻译：描述了用于高灵敏度和高速分析物检测的基于微粒的扩增（MBA）。 MBA基于通过使用包含附着在微粒表面上的多个位置上的多个信号分子的信号放大微粒而获得的信号放大，以及附着在多个位置上的多个分析物结合分子表面。每个信号分子又具有附着于其上的多个信号发射部分，例如吖啶鎓。这与分离微粒例如铁磁颗粒结合，该分离微粒也具有附着在表面上的分析物结合分子，从而形成包含分析物，信号放大微粒和分离微粒的络合物。复合物发出相对于样品中分析物分子量的多少倍放大的信号。具体实施方案包括用于检测细菌，抗原，抗体和核酸的方法。

92. 发明申请

US20040005718A1 Method of fixing biopolymers to a substrate using magnetic beads and biopolymer measuring equipment using the method 审中-公开
标题翻译：使用磁珠将生物聚合物固定到基底上的方法和使用该方法的生物聚合物测量设备
公开(公告)号：US20040005718A1
公开(公告)日：2004-01-08
申请号：US10395192
申请日：2003-03-25
申请人： YOKOGAWA ELECTRIC CORPORATION
发明人： Kazuhisa Fukushima
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6834 , C12Q1/6832 , Y10T436/25 , C12Q2563/143
摘要： According to the present invention, biopolymers are fixed to a substrate by attaching probe DNA to magnetic beads and fixing the above magnetic beads to the substrate by attracting them using a magnetic attraction. As described above, since magnetic beads with probe DNA attached are fixed to the substrate by attracting them using a magnetic force, the above-mentioned magnetic beads can be fixed with a force stronger than the conventional bonding of probe DNA with the substrate. Accordingly, the magnetic beads are not in danger of exfoliation from the substrate or washing-away of probe DNA during washing treatment.
摘要翻译：根据本发明，通过将探针DNA附着到磁珠上并将上述磁珠通过使用磁吸引物吸引而将上述磁珠固定在基板上，将生物聚合物固定在基板上。如上所述，由于附着有探针DNA的磁珠通过使用磁力吸引而固定在基板上，所以可以用比探针DNA与基板的常规结合更强的力来固定上述磁珠。因此，在洗涤处理期间，磁珠不具有从基材剥离的危险或探针DNA的洗脱。

93. 发明申请

US20030175706A1 Nucleic acid amplification methods 审中-公开
公开(公告)号：US20030175706A1
公开(公告)日：2003-09-18
申请号：US09978261
申请日：2001-10-15
发明人： David Y. Zhang
IPC分类号： C12P019/34 , C12Q001/68
CPC分类号： C12Q1/6858 , C12Q1/682 , C12Q1/6827 , C12Q1/6834 , C12Q2600/158 , C12Q2561/125 , C12Q2525/197 , C12Q2525/161 , C12Q2537/143 , C12Q2525/307 , C12Q2565/519 , C12Q2563/143 , C12Q2531/125 , C12Q2531/119 , C12Q2531/113
摘要： The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.

94. 发明申请

US20030165935A1 Digital assay 审中-公开
标题翻译：数字测定
公开(公告)号：US20030165935A1
公开(公告)日：2003-09-04
申请号：US10302688
申请日：2002-11-21
发明人： Charles S. Vann , Kai Q. Lao
IPC分类号： C12Q001/68
CPC分类号： C12Q1/6816 , B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q2563/155 , C12Q2561/125 , C12Q2545/114 , C12Q2565/102 , C12Q2563/143
摘要： The invention relates to methods and compositions for the detection of targets in a sample.
摘要翻译：本发明涉及用于检测样品中靶标的方法和组合物。

95. 发明授权

US06569620B1 Method for the automated generation of nucleic acid ligands 有权
标题翻译：自动生成核酸配体的方法
公开(公告)号：US06569620B1
公开(公告)日：2003-05-27
申请号：US09232946
申请日：1999-01-19
申请人： Larry Gold , Dominic A. Zichi , Robert D. Jenison , Daniel J. Schneider
发明人： Larry Gold , Dominic A. Zichi , Robert D. Jenison , Daniel J. Schneider
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12N15/115 , C12N2310/16 , C12N2330/31 , C12Q2541/101 , C12Q2563/143 , C12Q2563/149
摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a cartesian robotic manipulator controlled by a computer.
摘要翻译：本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的笛卡尔机器人操纵器执行。

96. 发明申请

US20030096987A1 Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same 有权
标题翻译：核酸可键合磁性载体和使用其分离核酸的方法
公开(公告)号：US20030096987A1
公开(公告)日：2003-05-22
申请号：US10202212
申请日：2002-07-22
申请人： Toyo Boseki Kabushiki Kaisha
发明人： Hiroaki Uematsu , Katsuya Daimon , Satoko Yoshiga
IPC分类号： C12Q001/68 , C07H021/04 , C04B035/64 , C04B035/26
CPC分类号： B82Y25/00 , B01D15/00 , B01D15/1807 , B01J20/103 , B01J20/28009 , B03C1/01 , C12N15/1013 , C12Q1/6806 , C12Q1/6844 , G01N33/54326 , H01F1/0054 , H01F1/0063 , H01F1/111 , C12Q2563/155 , C12Q2563/143
摘要： A nucleic acid-bondable magnetic carrier of the present invention is a magnetic silica particle comprising a superparamagnetic metal oxide, wherein the magnetic silica particle has a specific surface of about 100 to about 800 m2/g.
摘要翻译：本发明的可与核酸结合的磁性载体是包含超顺磁金属氧化物的磁性二氧化硅颗粒，其中磁性二氧化硅颗粒具有约100至约800m 2 / g的比表面积。

97. 发明申请

US20020182598A1 Nucleic acid amplification methods 有权
标题翻译：核酸扩增方法
公开(公告)号：US20020182598A1
公开(公告)日：2002-12-05
申请号：US09728265
申请日：2000-12-01
发明人： David Y. Zhang
IPC分类号： C12Q001/68 , C12P019/34
CPC分类号： C12P19/34 , C12Q1/682 , C12Q1/6834 , C12Q1/6862 , C12Q2537/137 , C12Q2533/107 , C12Q2531/125 , C12Q2563/143
摘要： The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.
摘要翻译：本发明涉及用于进行用于快速，自动检测感染性病原体和正常和异常基因的所述测定的测定和试剂盒。本发明还涉及基因组DNA和总mRNA的一般扩增方法以及使用本文公开的扩增方法分析差异mRNA表达的方法。

98. 发明申请

US20020155458A1 Nanoparticles having oligonucleotides attached thereto and uses therefor 有权
公开(公告)号：US20020155458A1
公开(公告)日：2002-10-24
申请号：US09967409
申请日：2001-09-28
申请人： Nanosphere, Inc.
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C12Q001/68
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

99. 发明申请

US20020146720A1 Nanoparticles having oligonucleotides attached thereto and uses therefor 有权
公开(公告)号：US20020146720A1
公开(公告)日：2002-10-10
申请号：US09961949
申请日：2001-09-20
申请人： Nanosphere, Inc.
发明人： Chad A. Mirkin , Robert L. Letsinger , Robert C. Mucic , James J. Storhoff , Robert Elghanian , Thomas A. Taton
IPC分类号： C12Q001/68
CPC分类号： B82Y30/00 , B01J2219/00274 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/00603 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , B82B1/00 , B82Y15/00 , C07B2200/11 , C07H21/00 , C12Q1/6813 , C12Q1/6816 , C12Q1/6818 , C12Q1/6825 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6839 , C40B40/06 , Y02A50/57 , Y10S977/924 , C12Q2563/155 , C12Q2563/143 , C12Q2537/143 , C12Q2525/197 , C12Q2565/625 , C12Q2537/125 , C12Q2525/161
摘要： The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

100. 发明授权

US06458540B1 Methods and compositions for detection of specific nucleotide sequences 失效
公开(公告)号：US06458540B1
公开(公告)日：2002-10-01
申请号：US09633848
申请日：2000-08-07
申请人： Elliot R. Ramberg
发明人： Elliot R. Ramberg
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6813 , C12Q1/6839 , C12Q1/689 , C12Q2525/107 , C12Q2537/119 , C12Q2521/319 , C12Q2525/125 , C12Q2521/301 , C12Q2561/108 , C12Q2563/149 , C12Q2563/143
摘要： Methods and compositions are provided for the detection of specific nucleic acid sequences purified from cellular or tissue sources. More particularly, the present invention includes methods and compositions for the detection of nucleic acid sequences using a protection molecule that forms a protected nucleic acid sequence (PNAS) such as a triplex or duplex nucleic acid structure that includes the target nucleic acid sequence. An assay using the methods of the present invention may include one, two or three levels of specificity to minimize false positive signals. An assay using the methods or compositions of the present invention can be performed on large amounts of purified DNA in a single test, with high levels of sensitivity, thus eliminating the need for DNA amplification procedures.

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