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91. 发明授权

US08147668B2 Apparatus for manipulating droplets 有权
标题翻译：用于操纵液滴的装置
公开(公告)号：US08147668B2
公开(公告)日：2012-04-03
申请号：US11585618
申请日：2006-10-23
申请人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair , Hong Ren
发明人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair , Hong Ren
IPC分类号： B03C5/02
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Apparatuses for mixing droplets, such as a binary mixing apparatus, are provided. The binary mixing apparatus includes an array of electrodes and a conducting element positioned in relation to at least one of the electrodes to enable a droplet placed in electrical communication with the at least one electrode to electrically communicate with the conducting element. The binary mixing apparatus additionally includes an electrode selector for sequentially biasing one or more selected electrodes of the array to move a droplet disposed on the array into contact with another droplet. The apparatus further includes a first droplet supply area communicating with the array and a second droplet supply area communicating with the array.
摘要翻译：提供了用于混合液滴的装置，例如二元混合装置。二进制混合装置包括电极阵列和相对于至少一个电极定位的导电元件，使得与至少一个电极电连通的液滴能够与导电元件电连通。二进制混合装置还包括用于顺序地偏置阵列的一个或多个所选择的电极的电极选择器，以移动设置在阵列上的液滴与另一液滴接触。该装置还包括与阵列连通的第一液滴供应区域和与阵列连通的第二液滴供应区域。

92. 发明申请

US20110100823A1 Droplet-Based Nucleic Acid Amplification Apparatus and System 有权
标题翻译：基于液滴的核酸扩增设备和系统
公开(公告)号：US20110100823A1
公开(公告)日：2011-05-05
申请号：US12960583
申请日：2010-12-06
申请人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
发明人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
IPC分类号： G01N21/76 , G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.
摘要翻译：本发明涉及一种基于液滴的核酸扩增装置和系统。根据一个实施例，使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器，该检测区域被光吸收，低荧光或非荧光材料涂覆。

93. 发明申请

US20100025242A1 APPARATUSES AND METHODS FOR MANIPULATING DROPLETS 有权
标题翻译：装置和方法用于操作喷雾器
公开(公告)号：US20100025242A1
公开(公告)日：2010-02-04
申请号：US12576794
申请日：2009-10-09
申请人： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
发明人： Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC分类号： B01D57/02
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
摘要翻译：公开了用于操纵液滴的装置和方法。在一个实施例中，提供了一种用于操纵液滴的装置，该装置包括一个基板，设置在该基板上的多个电极阵列，其中每个阵列中的对应电极连接到公共电信号，以及首先设置在基板上的电介质层侧表面并图案化以覆盖电极。

94. 发明授权

US07329545B2 Methods for sampling a liquid flow 有权
标题翻译：液体取样方法
公开(公告)号：US07329545B2
公开(公告)日：2008-02-12
申请号：US10253342
申请日：2002-09-24
申请人： Vamsee K. Pamula , Michael G. Pollack , Philip Y. Paik , Hong Ren , Richard B. Fair
发明人： Vamsee K. Pamula , Michael G. Pollack , Philip Y. Paik , Hong Ren , Richard B. Fair
IPC分类号： G01N35/08 , G01N35/00 , G01N1/00 , B01L3/02 , B32B5/02
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： In a method for sampling a continuous liquid flow, the liquid flow is supplied to a surface along an input flow path. The liquid flow is sampled by forming a sample droplet from a portion of the liquid flow. The sample droplet is moved along an analysis flow path to a processing area of the surface, where the sample droplet is processed. Discrete sample droplets are formed and moved using an electrowetting technique. A binary mixing apparatus and method are also provided. The apparatus comprises an array of electrodes, an electronic controller communicating with the electrodes, a sample droplet supply area communicating with the array, and an additive droplet supply area communicating with the array. The electronic controller alternately energizes and de-energizes selected electrodes to carry out droplet-to-droplet binary mixing operations to obtain one or more droplets having a target mixing ratio.
摘要翻译：在对连续液体流进行取样的方法中，液体流被输送到沿输入流路的表面。通过从液体流的一部分形成样品液滴来取样液体流。样品液滴沿着分析流动路径移动到表面的处理区域，在该处理区域处理样品液滴。使用电润湿技术形成并移动离散样品液滴。还提供了二元混合装置和方法。该装置包括电极阵列，与电极连通的电子控制器，与该阵列连通的样品液滴供应区域以及与该阵列连通的附加液滴供应区域。电子控制器交替地激励和去激活所选择的电极以进行液滴到液滴的二进制混合操作以获得具有目标混合比的一个或多个液滴。

95. 发明授权

US09243282B2 Droplet-based pyrosequencing 有权
标题翻译：基于液滴的焦磷酸测序
公开(公告)号：US09243282B2
公开(公告)日：2016-01-26
申请号：US13470919
申请日：2012-05-14
申请人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair , Peter B. Griffin
发明人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair , Peter B. Griffin
IPC分类号： C12Q1/68 , C12P19/34 , G01N21/00 , G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.
摘要翻译：本发明涉及基于液滴的焦磷酸测序，包括鉴定样品核酸中目标位置的碱基的方法。该方法包括：（a）提供包括包含固定在珠粒上的样品核酸的第一液滴的液滴微致动器; 和（b）在所述液滴微致动器上：（i）使所述第一液滴与一个或多个试剂液滴接触以产生第二液滴，其中所述一个或多个试剂液滴包括用于通过掺入来扩展样品核酸的双链部分的试剂目标位置的核苷酸; （ii）分裂第二液滴以产生包含珠粒的第三液滴和不含珠粒的第四液滴; 和（iii）测定第三液滴以确定核苷酸是否并入目标位置。

96. 发明授权

US08846410B2 Bead incubation and washing on a droplet actuator 有权
公开(公告)号：US08846410B2
公开(公告)日：2014-09-30
申请号：US14081376
申请日：2013-11-15
申请人： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
发明人： Michael G. Pollack , Vamsee K. Pamula , Ramakrishna Sista , Arjun Sudarsan
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , B01L3/00 , B03C1/02 , B01F13/00 , B01F11/00 , B01L7/00 , G01N35/10
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

97. 发明申请

US20130118901A1 Apparatuses and Methods for Manipulating Droplets 有权
公开(公告)号：US20130118901A1
公开(公告)日：2013-05-16
申请号：US13737433
申请日：2013-01-09
申请人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
发明人： Michael G. Pollack , Vamsee K. Pamula , Richard B. Fair
IPC分类号： G01N27/447
CPC分类号： G01N27/44769 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0645 , B01L2300/0663 , B01L2300/0819 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/1827 , B01L2400/0415 , B01L2400/0424 , B81B3/0021 , B81B2201/058 , B81C1/00119 , C12Q1/6846 , G01N27/447 , G01N27/44791 , G05D7/0694 , H01L21/6715 , Y10T436/2575
摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

98. 发明申请

US20130092539A1 DROPLET-BASED NUCLEIC ACID AMPLIFICATION METHOD AND APPARATUS 有权
标题翻译：基于滴液的核酸放大方法和装置
公开(公告)号：US20130092539A1
公开(公告)日：2013-04-18
申请号：US13680979
申请日：2012-11-19
申请人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
发明人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
IPC分类号： G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.
摘要翻译：本发明的实施例的方面涉及一种模拟器，其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器，以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统液滴微致动器包括包括用于进行液滴操作的电极的基板。本发明的实施例的其它方面涉及一种液滴操作故障排除装置。本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

99. 发明授权

US08313698B2 Droplet-based nucleic acid amplification apparatus and system 有权
标题翻译：基于液滴的核酸扩增仪和系统
公开(公告)号：US08313698B2
公开(公告)日：2012-11-20
申请号：US12960583
申请日：2010-12-06
申请人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
发明人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
IPC分类号： G01N21/64 , G01N21/00 , B01L3/00
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.
摘要翻译：本发明涉及一种基于液滴的核酸扩增装置和系统。根据一个实施例，使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器，该检测区域被光吸收，低荧光或非荧光材料涂覆。

100. 发明授权

US07851184B2 Droplet-based nucleic acid amplification method and apparatus 有权
标题翻译：基于液滴的核酸扩增方法和装置
公开(公告)号：US07851184B2
公开(公告)日：2010-12-14
申请号：US11639490
申请日：2006-12-15
申请人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
发明人： Michael G. Pollack , Philip Y. Paik , Vamsee K. Pamula
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification method and apparatus. According to one embodiment, a method of amplifying a nucleic acid in a biological sample is provided, wherein the method includes: (a) providing a system comprising a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator comprising: (i) a sample potentially comprising a target nucleic acid; (ii) a substrate comprising electrodes for conducting droplet operations; and (iii) one or more temperature control means arranged in proximity with one or more of the electrodes for heating a region of the droplet microactuator such that a droplet can be transported into the region for heating; (b) using droplet operations to combine on the droplet microactuator one or more amplification reagent droplets and one or more sample droplets to yield an amplification-ready droplet; and (c) thermal cycling the amplification-ready droplet sufficient to result in amplification of a target nucleic acid when present in the amplification-ready droplet.
摘要翻译：本发明涉及一种基于液滴的核酸扩增方法和装置。根据一个实施方案，提供了扩增生物样品中的核酸的方法，其中所述方法包括：（a）提供包括液滴微致动器的系统，所述液滴微致动器电子耦合到能够执行指令的处理器并由所述液体微致动器控制，所述液滴微致动器包括：（i）潜在地包含靶核酸的样品; （ii）包括用于进行液滴操作的电极的基板; 和（iii）设置在一个或多个电极附近的一个或多个温度控制装置，用于加热液滴微致动器的区域，使得液滴可以被运送到加热区域中; （b）使用液滴操作在液滴微致动器上组合一个或多个扩增试剂液滴和一个或多个样品液滴以产生扩增就绪液滴; 和（c）将放大准备好的液滴热循环到足以在存在于扩增即用液滴中时导致靶核酸的扩增。

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