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    • 94. 发明申请
    • PROCESS FOR PRODUCING L-AMINO ACID
    • 生产L-氨基酸的方法
    • US20120015409A1
    • 2012-01-19
    • US13148564
    • 2010-02-09
    • Kazuhiko TabataAkihiro Senoo
    • Kazuhiko TabataAkihiro Senoo
    • C12P13/04C12P13/08C12P13/12C12P13/22C12P13/06C12P13/14
    • C12P13/06C07K14/195C12P13/08C12P13/12C12P13/14C12P13/222
    • The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid. To be specific, the present invention provides a process for producing a L-amino acid, comprising culturing a microorganism wherein the activity of the protein described in any one of (1) to (3) below is higher than that of the parent strain in a medium to produce the L-amino acid and accumulate the L-amino acid in the medium, and then collecting the L-amino acid from the medium:(1) a protein comprising the amino acid sequence shown by any one of SEQ ID NOS:2, 4, 6 and 8 (2) a protein consisting of the amino acid sequence resulting from deletion, substitution or addition of one or more amino acids in the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity (3) a protein consisting of the amino acid sequence having 80% or more homology to the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity.
    • 本发明提供了提高L-氨基酸的发酵生产效率的方法。 具体而言,本发明提供了L-氨基酸的制造方法,其特征在于,含有下述(1)〜(3)中任一项所述的蛋白质的活性高于母体菌株的活性的微生物 产生L-氨基酸并在培养基中积累L-氨基酸的培养基,然后从培养基中收集L-氨基酸:(1)包含SEQ ID NOS中任一个所示的氨基酸序列的蛋白质 2,4,6和8(2)由SEQ ID NO:2,4,4,7所示的氨基酸序列中的一个或多个氨基酸的缺失,取代或添加得到的氨基酸序列组成的蛋白质, 6和8,并且具有L-氨基酸转运活性(3)由与SEQ ID NO:2,4,6和8中任一个所示的氨基酸序列具有80%或更高同源性的氨基酸序列组成的蛋白质 并具有L-氨基酸转运活性。
    • 96. 发明申请
    • METHOD FOR PRODUCTION OF L-GLUTAMINE
    • L-谷氨酰胺的生产方法
    • US20110124059A1
    • 2011-05-26
    • US12439300
    • 2007-08-30
    • Yugo AdachiMikiro HayashiAkihiro SenooYoshiyuki YonetaniShin-ichi Hashimoto
    • Yugo AdachiMikiro HayashiAkihiro SenooYoshiyuki YonetaniShin-ichi Hashimoto
    • C12P13/14C12N1/21
    • C12N9/1205C12N9/0008C12P13/14
    • According to the present invention, it is possible to provide a microorganism belonging to the genus Corynebacterium, wherein the activity of (1) a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, or (2) a protein having a homology of 80% or more to the amino acid sequence shown by any one of SEQ ID NO:1 to 3, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by any one of SEQ ID NO:1 to 3, has been reduced or lost, and wherein the activity of (3) a protein having the amino acid sequence shown by SEQ ID NO:4, or (4) a protein having a homology of 80% or more to the amino acid sequence shown by SEQ ID NO:4, and having substantially the same activity as the activity of a protein having the amino acid sequence shown by SEQ ID NO:4, has been reduced or lost, and a process for producing L-glutamine using the microorganism and the like.
    • 根据本发明,可以提供属于棒状杆菌属的微生物,其中(1)具有SEQ ID NO:1〜3或(2)中任一项所示的氨基酸序列的蛋白质的活性, 与SEQ ID NO:1〜3中任一项所示的氨基酸序列具有80%以上的同源性的蛋白质,并且具有与具有下述任一项所示的氨基酸序列的蛋白质的活性基本上相同的活性 SEQ ID NO:1至3已经被还原或丧失,并且其中(3)具有SEQ ID NO:4所示氨基酸序列的蛋白质的活性,或(4)具有80%同源性的蛋白质或 更多地与SEQ ID NO:4所示的氨基酸序列相比,具有与SEQ ID NO:4所示的氨基酸序列的蛋白质的活性基本上相同的活性已被减少或丧失, 使用微生物等的L-谷氨酰胺。