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1. 发明授权

US08552177B2 Room temperature stable agarose solutions 有权
标题翻译：室温稳定的琼脂糖溶液
公开(公告)号：US08552177B2
公开(公告)日：2013-10-08
申请号：US13221439
申请日：2011-08-30
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： C08B37/02 , C08B37/00 , C07H1/00 , C07H3/00
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： A method for making functionalized porous crosslinked polysaccharide gel coated structures used as liquid chromatography media is provided. The method includes impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing water, 0.1% to 20% of a polysaccharide, 18% to 54% of a gel-inhibiting agent to prevent the gel from re-gelling, and 0.001% to 10% of an anionic fluorosurfactant for optimum solution coatability onto the substrate, each concentration is by total weight of the aqueous solution. Next water is evaporated from the coating, followed by exposing the dehydrated coating to a gelling agent thereby forming a porous polysaccharide gel coated substrate. Next the gel coated substrate is exposed to a crosslinking agent forming a porous crosslinked polysaccharide gel coated substrate. Next, the gel coated substrate is functionalized by contacting the porous polysaccharide gel coated substrate with sodium 3-bromopropanesulfonate, thereby attaching sulfopropyl ligands to the gel coated substrate, resulting in a functionalized porous absorptive polysaccharide gel coated chromatography structure. When the functionalized gel coated chromatography structure is used as liquid chromatography media, and a protein (e.g. lysozyme) containing solution contacts the media, the sulfopropyl ligands attached to the gel coated chromatography structure will bond to the protein, thereby removing the protein from the protein containg solution.
摘要翻译：提供了用作液相色谱介质的功能化多孔交联多糖凝胶涂层结构的方法。该方法包括用含有水，0.1％至20％的多糖，18％至54％的凝胶抑制剂的室温稳定的水性多糖溶液浸渍多孔基材以防止凝胶重新凝胶化，并且0.001％至10％的阴离子含氟表面活性剂，用于在基材上获得最佳的溶液涂布性，每种浓度均为水溶液的总重量。接下来的水从涂层中蒸发，然后将脱水涂层暴露于胶凝剂，从而形成多孔多糖凝胶涂覆的基质。接下来，将凝胶涂覆的基底暴露于形成多孔交联多糖凝胶涂覆的基底的交联剂。接下来，通过使多孔多糖凝胶涂覆的基质与3-溴丙磺酸钠接触使凝胶涂覆的底物功能化，从而将磺丙基配体连接到凝胶涂覆的基质上，得到官能化的多孔吸收多糖凝胶涂层色谱结构。当将功能化凝胶涂层色谱结构用作液相色谱介质，并且含有蛋白质（例如溶菌酶）的溶液与培养基接触时，附着在凝胶涂层色谱结构上的磺丙基配体将与蛋白质结合，从而从蛋白质中除去蛋白质包含解决方案

2. 发明授权

US09295928B2 Porous adsorptive or chromatographic media 有权
公开(公告)号：US09295928B2
公开(公告)日：2016-03-29
申请号：US12925915
申请日：2010-11-02
申请人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01J20/28 , B01D15/20 , B01J20/286 , B01J20/32 , B01J20/26 , B01J20/30 , B01D15/26 , B01D15/36
CPC分类号： B01D15/20 , B01D15/265 , B01D15/361 , B01J20/26 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28097 , B01J20/286 , B01J20/3064 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/54 , B01J2220/58 , B01J2220/62
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.

3. 发明申请

US20110311762A1 ROOM TEMPERATURE STABLE AGAROSE SOLUTIONS 有权
标题翻译：室温稳定的AGAROSE解决方案
公开(公告)号：US20110311762A1
公开(公告)日：2011-12-22
申请号：US13221439
申请日：2011-08-30
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： B32B3/12 , B32B3/26 , B32B5/02 , B05D5/00 , B05D3/10
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： The present invention provides porous functionalized crosslinked polysaccharide hydrogel coated structures and methods for making the same. The methods include impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing, water, a polysaccharide having a concentration in the aqueous solution from about 0.1% to about 20%, a gel-inhibiting agent having a concentration in the aqueous solution from about 18 to about 54%, and an anionic fluorosurfactant having a concentration in the aqueous solution from about 0.001% to about 10% by total weight of the aqueous solution. Followed by evaporating the water from the polysaccharide coating; and exposing the dehydrated polysaccharide coating to a gelling agent form a porous polysaccharide hydrogel coated substrate. The hydrogel coated substrate is exposed to a crosslinking agent to form a porous crosslinked polysaccharide hydrogel coated substrate, which is then functionalized by the attachment of a ligand.
摘要翻译：本发明提供多孔官能化交联多糖水凝胶涂层结构及其制备方法。所述方法包括用室温稳定的水性多糖溶液浸渍多孔基材，所述水性多糖溶液含有水，浓度在约0.1％至约20％的水溶液中的浓度为约0.1％至约20％的浓度的凝胶抑制剂，约18至约54％的阴离子含氟表面活性剂和在水溶液中的浓度的水溶液的总重量的约0.001％至约10％的阴离子含氟表面活性剂。随后从多糖涂层蒸发水; 并将脱水的多糖涂层暴露于胶凝剂形成多孔多糖水凝胶涂覆的基质。将水凝胶涂覆的基底暴露于交联剂以形成多孔交联的多糖水凝胶涂覆的底物，然后通过配体的附着将其官能化。

4. 发明授权

US07824548B2 Porous adsorptive or chromatographic media 有权
标题翻译：多孔吸附或色谱介质
公开(公告)号：US07824548B2
公开(公告)日：2010-11-02
申请号：US11821059
申请日：2007-06-21
申请人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01D15/08
CPC分类号： B01D15/20 , B01D15/265 , B01D15/361 , B01J20/26 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28097 , B01J20/286 , B01J20/3064 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/54 , B01J2220/58 , B01J2220/62
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
摘要翻译：能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附聚合物涂层的多孔基底，其包含基底孔隙体积的约1至约80％。得到的底物具有良好的对流和扩散流量和容量。底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

5. 发明申请

US20100196589A1 Room temperature stable agarose solutions 有权
公开(公告)号：US20100196589A1
公开(公告)日：2010-08-05
申请号：US12661979
申请日：2010-03-26
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： B05D5/00
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： Room temperature stable, non-gelling polysaccharide solutions such as agaroses, dextrans and cyclodextrans are made by the present invention. It has been found that by incorporating certain gel-inhibiting additives into an aqueous polysaccharide solution, the gel point is reduced or eliminated and the solution remains liquid at room temperature indefinitely. Additives that have been found to work include salts, such as lithium chloride and zinc chloride and bases, such as sodium hydroxide and lithium hydroxide. Mixtures of said salts and said bases can also be used with the same desired results. The composition of these solutions of the present idea can be further modified to include other additives, such as organic co-solvents or non-solvents, pH modifiers, surfactants or other polymers to customize the properties of the solution to improve the processability for the desired application and to form structures such as films, beads and coated porous substrates.

6. 发明申请

US20110049042A1 Porous adsorptive or chromatographic media 有权
标题翻译：多孔吸附或色谱介质
公开(公告)号：US20110049042A1
公开(公告)日：2011-03-03
申请号：US12925915
申请日：2010-11-02
申请人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01D15/00
CPC分类号： B01D15/20 , B01D15/265 , B01D15/361 , B01J20/26 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28097 , B01J20/286 , B01J20/3064 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/54 , B01J2220/58 , B01J2220/62
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
摘要翻译：能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附聚合物涂层的多孔基底，其包含基底孔隙体积的约1至约80％。所得到的底物具有良好的对流和扩散流量和容量。底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

7. 发明授权

US07687619B2 Room temperature stable agarose solutions 有权
标题翻译：室温稳定的琼脂糖溶液
公开(公告)号：US07687619B2
公开(公告)日：2010-03-30
申请号：US11050243
申请日：2005-02-03
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： C08B37/18 , C08B37/02 , C08B37/00 , C07H23/00 , C07H1/00
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： Room temperature stable, non-gelling polysaccharide solutions such as agaroses, dextrans and cyclodextrans are made by the present invention. It has been found that by incorporating certain gel-inhibiting additives into an aqueous polysaccharide solution, the gel point is reduced or eliminated and the solution remains liquid at room temperature indefinitely. Additives that have been found to work include salts, such as lithium chloride and zinc chloride and bases, such as sodium hydroxide and lithium hydroxide. Mixtures of said salts and said bases can also be used with the same desired results. The composition of these solutions of the present idea can be further modified to include other additives, such as organic co-solvents or non-solvents, pH modifiers, surfactants or other polymers to customize the properties of the solution to improve the processability for the desired application and to form structures such as films, beads and coated porous substrates.
摘要翻译：通过本发明制备室温稳定的非胶凝多糖溶液，如琼脂糖，葡聚糖和环糊精。已经发现，通过将某些凝胶抑制添加剂掺入多糖水溶液中，凝胶点被减少或消除，溶液在室温下保持液体无限期。已发现工作的添加剂包括盐，如氯化锂和氯化锌，以及碱，如氢氧化钠和氢氧化锂。所述盐和所述碱的混合物也可以以相同的期望结果使用。本发明的这些溶液的组成可进一步修饰，以包括其它添加剂，如有机共溶剂或非溶剂，pH调节剂，表面活性剂或其他聚合物，以定制溶液的性质，以改善所需溶剂的加工性能并且形成诸如膜，珠和涂覆的多孔基底的结构。

8. 发明授权

US07479223B2 Porous adsorptive or chromatographic media 有权
标题翻译：多孔吸附或色谱介质
公开(公告)号：US07479223B2
公开(公告)日：2009-01-20
申请号：US11050004
申请日：2005-02-03
申请人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01D15/08
CPC分类号： B01J20/286 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28085 , B01J20/3035 , B01J20/305 , B01J20/321 , B01J20/3251 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J2220/62 , B01J2220/82
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be crosslinked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
摘要翻译：能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附性涂层的多孔基材，其包含基材孔隙体积的约1至约80％。所得到的底物具有良好的对流和扩散流量和容量。底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

9. 发明授权

US07479222B2 Porous adsorptive or chromatographic media 有权
标题翻译：多孔吸附或色谱介质
公开(公告)号：US07479222B2
公开(公告)日：2009-01-20
申请号：US11049803
申请日：2005-02-03
申请人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
发明人： Anthony J. DiLeo , Justin McCue , Wilson Moya , Igor Quinones-Garcia , Neil P. Soice , Volkmar Thom , Sarah Yuan
IPC分类号： B01D15/08
CPC分类号： B01D15/20 , B01D15/265 , B01D15/361 , B01J20/26 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/28078 , B01J20/28097 , B01J20/286 , B01J20/3064 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/3272 , B01J20/3274 , B01J20/328 , B01J20/3282 , B01J20/3289 , B01J20/3293 , B01J2220/54 , B01J2220/58 , B01J2220/62
摘要： A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
摘要翻译：能够吸附过滤具有多孔自支撑基底和一种或多种多孔吸附聚合物涂层的多孔基底，其包含基底孔隙体积的约1至约80％。所得到的底物具有良好的对流和扩散流量和容量。底物可以是交联的，具有一个或多个捕获化学物质连接到其上，并且可用作用于选择性过滤所需物质（包括生物分子例如蛋白质和DNA片段）的色谱介质。

10. 发明授权

US08008476B2 Room temperature stable agarose solutions 有权
标题翻译：室温稳定的琼脂糖溶液
公开(公告)号：US08008476B2
公开(公告)日：2011-08-30
申请号：US12661979
申请日：2010-03-26
申请人： Wilson Moya , Neil P. Soice
发明人： Wilson Moya , Neil P. Soice
IPC分类号： C08B37/02 , C07H1/00
CPC分类号： C08J3/09 , C08J2305/00 , C08L5/02 , C08L5/12 , D06M11/13 , D06M11/155 , D06M11/38 , D06M15/03 , D06M23/10 , Y10T428/24149 , Y10T428/249953 , Y10T442/20
摘要： The present invention provides methods for making porous crosslinked polysaccharide hydrogel coated structures. The methods include impregnating a porous substrate with a room temperature stable aqueous polysaccharide solution containing, water, a polysaccharide having a concentration in the aqueous solution from about 0.1% to about 20%, a gel-inhibiting agent having a concentration in the aqueous solution from about 18 to about 54%, and an anionic fluorosurfactant having a concentration in the aqueous solution from about 0.001% to about 10% by total weight of the aqueous solution. Followed by evaporating the water from the polysaccharide coating; and exposing the dehydrated polysaccharide coating to a gelling agent form a porous polysaccharide hydrogel coated substrate. The hydrogel coated substrate is exposed to a crosslinking agent to form a porous crosslinked polysaccharide hydrogel coated substrate.
摘要翻译：本发明提供制备多孔交联多糖水凝胶涂层结构的方法。所述方法包括用室温稳定的水性多糖溶液浸渍多孔基材，所述水性多糖溶液含有水，浓度在约0.1％至约20％的水溶液中的浓度为约0.1％至约20％的浓度的凝胶抑制剂，约18至约54％的阴离子含氟表面活性剂和在水溶液中的浓度的水溶液的总重量的约0.001％至约10％的阴离子含氟表面活性剂。随后从多糖涂层蒸发水; 并将脱水的多糖涂层暴露于胶凝剂形成多孔多糖水凝胶涂覆的基质。将水凝胶涂覆的基底暴露于交联剂以形成多孔交联的多糖水凝胶涂覆的基底。

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